The urinary levels of type II collagen peptide COLL 2-1 and its nitrated form (COLL 2-1 NO2) are correlated with the clinical severity of knee osteoarthritis

in Osteoporosis International (2002, November), 13(Suppl.3), 54

Development of new immunoassays for the quantification of inflammatory related cartilage degradation

in Osteoporosis International (2002, November), 13(Suppl. 3), 54

Avocado/soybean unsaponifiables increase aggrecan synthesis and reduce catabolic and pro-inflammatory mediators production by human osteoarthritic chondrocytes

in Osteoporosis International (2002, November), 13(Suppl.3), 53

Characterization of the Metabolism of Osteoarthritic Chondrocytes Cultured in Alginate Beads

in Osteoarthritis and Cartilage (2002), 10(SA), 34

Avocado/soybean unsaponifiables increase aggrecan synthesis and reduce catabolic and pro-inflammatory mediators production by human osteoarthritic chondrocytes

in Osteoarthritis and Cartilage (2002), 10(Suppl A), 34

Diacerein by its Metabolite Rhein, Down-Regulates Stromelysins and Nitric Oxide Productions and Up-Regulates Aggrecan Production by Human Chondrocytes in Alginate Beads

in Tissue Engineering (2001), 7(5), 648

Long-term Effects of Nonsteroidal Antiinflammatory Drugs on Human Chondrocytes in Alginate Beads

in Annals of the Rheumatic Diseases (2001), 60(suppl1), 284

Long-term Effects of Nonsteroidal Antiinflammatory Drugs on Human Chondrocytes in Alginate Beads

in Osteoarthritis and Cartilage (2001), 9(supplB), 32

Diacerein by its Metabolite Rhein, Down-Regulates Stromelysins and Nitric Oxide Productions and Up-Regulates Aggrecan Production by Human Chondrocytes in Alginate Beads

in Clinical Rheumatology (2001), 20(5), 414

Highly Specific Radioimmunoassay for Human Insulin Based on Immune Exclusion of All Insulin Precursors

in Clinical Chemistry (1998), 44(7), 1504-13

We describe a rapid and simple insulin RIA in which proinsulin and conversion intermediates do not interfere. Three monoclonal antibodies (S1, S2, and S53) were selected for their specificity (directed ... [more ▼]

We describe a rapid and simple insulin RIA in which proinsulin and conversion intermediates do not interfere. Three monoclonal antibodies (S1, S2, and S53) were selected for their specificity (directed, respectively, against the B10 region, the junction between A chain and C-peptide, and the junction between B chain and C-peptide), their affinity constant (approximately 10(10) L/mol), and their interactive properties in mixture. S2 and S53 were able to bind simultaneously to the same proinsulin molecule, whereas neither could bind simultaneously with S1. Preincubation of serum samples with an excess of S2 resulted in capture of proinsulin and conversion intermediates modified at the junction between B chain and C-peptide into immune complexes that no longer reacted with S1. Similarly, preincubation with S53 prevented proinsulin and conversion intermediates modified at the junction between A chain and C-peptide from reacting with S1. Preincubation with an excess of both S2 and S53 left insulin as the sole reactant with S1. Thus, separation of insulin precursors from insulin by mutually exclusive antibodies is feasible, and on the basis of this new principle, a highly specific RIA for insulin was designed. The detection limit was 11 pmol/L, and the inter- and intraassay coefficients of variation were 11% and 5%, respectively. The potential of the assay for use in clinical studies was verified by application to serum samples from control subjects and patients with diabetes or insulinoma. [less ▲]