References of "De Pauw, Edwin"
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See detailLevels of PCDDs, PCDFs and PCBs in Belgian and international fast food samples
Focant, Jean-François ULg; Pirard, Catherine; De Pauw, Edwin ULg

in Chemosphere (2004), 54(1), 137-142

Congener-specific analyses of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) were performed on twenty-eight non-pooled fast food ... [more ▼]

Congener-specific analyses of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) were performed on twenty-eight non-pooled fast food samples collected in Belgium, Switzerland, Czech Republic, United States of America and Australia. PCDD/F and PCB concentrations for the four investigated types of meals were very low. PCDD/F values ranged from non-detected to 1.40 pg WHO-TEQ/g fat and from 0.79 to 2.08 pg WHO-TEQ/g fat for lower and upper bound, respectively. Major contributors to the PCDD/F TEQ were 1,2,3,4,7,8-HxCDD, 1,2,3,6,7,8-HxCDD, 2,3,7,8-TCDF and 2,3,4,7,8-PeCDF. The relative contribution of PCBs to the total TEQ was 68%. For adults, an average estimated intake was 6.7 pg WHO-TEQ/kg bw/month, including consumption of all types of analyzed meals, representing 9.5% of the PTMI. For child, a value of 14.5 pg WHO-TEQ/kg bw/month was obtained, representing 20.6% of the PTMI. (C) 2003 Elsevier Ltd. All rights reserved. [less ▲]

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See detailIdentification of specific biomarkers related to the effects of pollutants on the immune system of marine mammals
Brenez, Cécile; Gerkens, Pascal; Jauniaux, Thierry ULg et al

Poster (2004)

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See detailHigh-throughput analysis of human serum for selected polychlorinated biphenyls (PCBs) by gas chromatography-isotope dilution time-of-flight mass spectrometry (GC-IDTOFMS)
Focant, Jean-François ULg; Cochran, Jack W.; Dimandja, Jean-Marie D. et al

in Analyst (2004), 129(4), 331-336

A method for the high-throughput analysis of human serum for the 38 most prevalent polychlorinated biphenyls (PCBs) based on the use of fast gas chromatography-isotopic dilution time-of-flight mass ... [more ▼]

A method for the high-throughput analysis of human serum for the 38 most prevalent polychlorinated biphenyls (PCBs) based on the use of fast gas chromatography-isotopic dilution time-of-flight mass spectrometry (GC-IDTOFMS) is presented. The chromatographic separation time was 8 min. The separation of the congeners was carried out either chromatographically or analytically using the mass spectral deconvolution capability of the TOFMS. The instrument and the method limits of detection (LODs) were 0.5 pg muL(-1) and 20 pg muL(-1), respectively, which is not as good as the one achieved using high resolution mass spectrometry (HRMS) but allows the detection and quantification of the prevalent PCBs present in real human serum samples. The dynamic range covered 3 orders of magnitude. The comparison with the high resolution mass spectrometry (HRMS) reference method (28 min) was good and some separation improvements have been observed. This method allows the analysis of 100 samples per day per instrument. [less ▲]

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See detailContribution of tobacco smoking to dioxin accumulation: opposite effects according to gender
Fierens, Sébastian; Eppe, Gauthier ULg; Focant, Jean-François ULg et al

in Organohalogen Compounds (2004), 66

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See detailMeasurement of dioxins and WHO PCBs in foodstuffs using GCxGC-IDTOFMs
Focant, Jean-François ULg; Pirard, Catherine; Massart, Anne-Cécile ULg et al

in Organohalogen Compounds (2004), 66

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See detailAssessment of the impact of an old MSWI. Part1 : Level of PCDD/Fs and PCBs in surrounding soils and eggs
Pirard, Catherine; Focant, Jean-François ULg; Massart, Anne-Cécile ULg et al

in Organohalogen Compounds (2004), 66

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See detailDR-CALUX((R)) screening of food samples: evaluation of the quantitative approach to measure dioxin, furans and dioxin-like PCBs
Scippo, Marie-Louise ULg; Eppe, Gauthier ULg; De Pauw, Edwin ULg et al

in Talanta (2004), 63(5), 1193-1202

European legislation laid down maximum tolerable levels of dioxin in feed and food as well as analytical method requirements. In order to face with large monitoring programs, it was foreseen in the EU ... [more ▼]

European legislation laid down maximum tolerable levels of dioxin in feed and food as well as analytical method requirements. In order to face with large monitoring programs, it was foreseen in the EU strategy to integrate screening methods, using either a qualitative (screening) approach, or a quantitative approach. In this study, dioxin results obtained using the Dioxin Responsive Chemical-Activated LUciferase gene eXpression (DR-CALUX(R)) cell-based assay (quantitative approach), were compared with gas chromatography-high resolution mass spectrometry (GC-HRMS) analyses data. Instead of using World Health Organization-toxic equivalent factor (WHO-TEF), the comparison was based on the assessment of relative effective potencies (REPs) for each congener of the 17 toxic 2,3,7,8-polychlorodibenzo-p-dioxins/furans (PCDD/Fs) and 12 dioxin-like polychlorobiphenyls (DL-PCBs). According to published data, DR-CALUX(R)-REP evaluated here appear similar to WHO-TEF for PCDD/Fs while lower values were observed for DL-PCBs. We analyzed two "home made" contaminated fat samples, displaying both the same WHO-toxic equivalent quantities (WHO-TEQ) concentration (12 pg WHO-TEQ g(-1)). They were spiked with either a low or a high amount of DL-PCBs. In both cases, the DR-CALUX(R) measured concentration (picogram 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) eq. g(-1)) corresponded to the PCDD/Fs WHO-TEQ concentration only. A good agreement was nevertheless found between the DR-CALUX(R) measurements and the recalculated DR-CALUX(R)-TEQ contents (using DR-CALUX(R)-REP instead of WHO-TEF), demonstrating that the observed response was due, in both cases, to the addition of the responses of the standards added to the fat. By contrast, in real contaminated samples (feed or cod liver samples), DR-CALUX(R) measured concentrations were similar to WHO-TEQ GC-HRMS measured concentrations. But, depending on the PCDD/Fs and DL-PCBs congener content, the DR-CALUX(R) measured concentrations were either lower or higher than calculated DR-CALUX(R)-TEQ contents, demonstrating that possible co-extracted contaminants contributed to the CALUX response. Owing to these divergences, the quantitative determination of dioxin-like content in food and feed using CALUX as screening method is questionable, except for samples displaying constant congener patterns, in which cases, correction factors could be applied. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailInvestigation of non-covalent interactions between paramagnetic complexes and human serum albumin by electrospray mass spectrometry
Henrotte, Virginie; Laurent, Sophie ULg; Gabelica, Valérie ULg et al

in Rapid Communications in Mass Spectrometry : RCM (2004), 18(17), 1919-1924

Stable gadolinium(III) chelates are nowadays routinely used as contrast agents for magnetic resonance imaging (MRI). Their non-covalent binding to human serum albumin (HSA) has shown to improve their ... [more ▼]

Stable gadolinium(III) chelates are nowadays routinely used as contrast agents for magnetic resonance imaging (MRI). Their non-covalent binding to human serum albumin (HSA) has shown to improve their efficacy. Non-covalent interactions lead to complex formation that can be quantified by several techniques that are mostly tedious and time-consuming. In this study, electrospray ionization mass spectrometry (ESI-MS) was used to investigate the interaction between HSA and several gadolinium(III) complexes. The results were compared with those obtained in the liquid phase. Four gadolinium complexes were investigated: Gd-DTPA 1, Gd-C4Me-DTPA 2, Gd-EOB-DTPA 3, and MP-2269 4. Relaxometry studies show that complexes 1 and 2 have no significant affinity for HSA, while complexes 3 and 4 have increasing affinities for the protein. 1:1 and 1:2 complexes between HSA and MP-2269 were detected by ESI-MS for a twofold excess of the contrast agent, whereas a ligand/protein molar ratio of 4:1 was necessary to observe a 1:1 stoichiometry for Gd-EOB-DTPA, an observation that is in good agreement with the known weaker affinity of the contrast agent for the protein. At a fourfold molar excess, no supramolecular complex was observed for Gd-DTPA I and Gd-C4Me-DTPA 2; a tenfold molar excess was necessary to detect a 1:1 complex, confirming the very weak affinity of these contrast agents for HSA. [less ▲]

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See detailInfluence of the capillary temperature and the source pressure on the internal energy distribution of electrosprayed ions
Gabelica, Valérie ULg; De Pauw, Edwin ULg; Karas, Michael

in International Journal of Mass Spectrometry (2004), 231(2-3), 189-195

The internal energy distributions in a heated capillary nano-electrospray source have been determined using the "survival yield" method. At low capillary temperatures, the internal energy distribution is ... [more ▼]

The internal energy distributions in a heated capillary nano-electrospray source have been determined using the "survival yield" method. At low capillary temperatures, the internal energy distribution is characterized by a low-energy tail, which can be attributed to a fraction of ions not fully desolvated in the heated capillary. This low-energy tais is shown to disappear when the source pressure is increased. This explains why increased source pressure is favorable in the case of highly hydrophilic compounds or non-covalent complexes in order to achieve sufficient desolvation without fragmentation. It is also shown that "high temperature-low voltage" are not equivalent to "low temperature-high voltage" source conditions. These observations are important for fundamental issues as well as for source-CID mass spectral library searching applications. [less ▲]

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See detailComparison of the rat and mouse cell lines commercially available for CALUX bioassays
Goeyens, Leo; Windal, Isabelle; Scippo, Marie-Louise ULg et al

in Organohalogen compounds (2004), 66

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See detailLevels of contamination for various pollutants present in Belgian human plasma
Van Wouwe, Nathalie; Covaci, Adrian; Kannan, Kurunthachalam et al

in Organohalogen Compounds (2004)

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See detailDIOXIN BODY BURDEN AMONG BLOOD DONORS BEFORE AND AFTER THE BELGIAN DIOXIN/PCB INCIDENT
Debacker, Noemi; Sasse, André; Van Wouwe, Nathalie et al

in Organohalogen Compounds (2004)

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See detailApplication of the CALUX bioassay for epidemiological study: analyses of Belgian human plasma
Van Wouwe, Nathalie; Debacker, Noemi; Sasse, André et al

in Organohalogen Compounds (2004)

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See detailIdentification of specific biomarkers related to the effects of pollutants on the immune system of marine mammals
Brenez, Cécile; Gerkens, Pascal; Jauniaux, Thierry ULg et al

Poster (2003, December)

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See detailDioxin/polychlorinated biphenyl body burden, diabetes and endometriosis: findings in a population-based study in Belgium
Fierens, S.; Mairesse, H.; Heilier, J. F. et al

in Biomarkers : Biochemical Indicators of Exposure, Response, & Susceptibility to Chemicals (2003), 8(6), 529-534

Dioxins and polychlorinated biphenyls (PCBs) are persistent organic pollutants widely distributed in the food chain, which is the main source of human exposure. Their effects on human health at background ... [more ▼]

Dioxins and polychlorinated biphenyls (PCBs) are persistent organic pollutants widely distributed in the food chain, which is the main source of human exposure. Their effects on human health at background exposure levels are still poorly understood. Recent epidemiological evidence suggests a possible association between these pollutants and diabetes. We report here the results of a population-based study in Belgium on 257 (142 women and 115 men) environmentally exposed subjects, including 10 cases of endometriosis and nine cases of diabetes. Seventeen 2,3,7,8-polychlorinated dibenzodioxins/dibenzofurans (PCDD/Fs or dioxins), four coplanar PCBs (International Union of Pure and Applied Chemistry [IUPAC] nos 77, 81, 126 and 169) and 12 PCB markers ( IUPAC nos 3, 8, 28, 52, 101, 118, 138, 153, 180, 194, 206 and 209) were quantified in serum fat from fasting blood samples in order to estimate the body burden of these pollutants. Whilst no difference was found between women with endometriosis and their controls, diabetic patients had significantly increased serum levels of dioxins, coplanar PCBs and the 12 PCB markers. After adjustment for age and other covariates, serum total toxic equivalent activity (sum of PCDD/Fs and coplanar PCBs) and 12 PCB marker concentrations in diabetics were 62% (p=0.0005) and 39% (p=0.0067) higher, respectively, than in controls. The risk of diabetes was significantly increased in subjects in the top decile for adjusted concentrations of dioxins (odds ratio 5.1, 95% confidence interval [CI] 1.18-21.7), coplanar PCBs (odds ratio 13.3, 95% CI 3.31-53.2) or 12 PCB markers (odds ratio 7.6, 95% CI 1.58-36.3). These findings warrant further studies to assess the significance of the associations between diabetes and environmental exposure to polychlorinated pollutants. [less ▲]

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