References of "De Pauw, Edwin"
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See detailComprehensive Analysis of PBDEs, PCDD/Fs and PCBs
PIRARD, Catherine ULg; De Pauw, Edwin ULg; Focant, Jean-François ULg

Conference (2003, August)

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See detailDioxin accumulation in residents around incinerators
Fierens, S.; Mairesse, H.; Hermans, C. et al

in Journal of Toxicology and Environmental Health. Part A (2003), 66(14), 1287-1293

To evaluate the human exposure impact of municipal waste incinerators, dioxin and coplanar polychlorinated biphenyl (PCB) concentrations were determined in blood of 84 subjects who resided approximately ... [more ▼]

To evaluate the human exposure impact of municipal waste incinerators, dioxin and coplanar polychlorinated biphenyl (PCB) concentrations were determined in blood of 84 subjects who resided approximately 18 yr in the vicinity of two old incinerators, one located in a rural area (n = 51) and the other in an industrial area (n = 33). These subjects were compared with 63 controls from an unpolluted area. While no change was found in contaminant levels in residents living around the incinerator in the industrial area, subjects residing around the incinerator in the rural area possessed significantly higher serum levels of dioxins (38 vs. 24 pg TEQ/g fat) and coplanar PCBs (10 vs. 7 pg TEQ/g fat) than controls. These results were confirmed by multiple-regression analysis, showing that residence around the incinerator in the rural area (partial r(2) = .18) was the major contributor to dioxin accumulation followed by age (partial r(2) = .07). A two-way analysis of variance (ANOVA) on age-adjusted dioxin levels revealed a significant interaction between residence around incinerators and the consumption of fat from local origin, especially bovine and poultry products. Although age-adjusted dioxin levels in controls did not vary with local animal fat consumption, concentrations of dioxins in subjects living around the incinerators increased proportionally to their intake of local animal fat, with almost a doubling in subjects with a fat intake higher than 150 gfat/wk. Extrapolation from these data suggests that a significant increase of dioxin body burden is likely to occur only when dioxin emissions exceed 5 ng TEQ/Nm(3), a threshold considerably above most emissions standards currently in force. [less ▲]

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See detailSurvey of commercial pasteurised cows' milk in Wallonia (Belgium) for the occurrence of polychlorinated dibenzo-p-dioxins, dibenzofurans and coplanar polychlorinated biphenyls
Focant, Jean-François ULg; Pirard, Catherine; Massart, Anne-Cécile ULg et al

in Chemosphere (2003), 52(4), 725-733

Congener-specific analyses of 7 polychlorinated dibenzo-p-dioxins (PCDDs), 10 polychlorinated dibenzofurans (PCDFs) and 4 non-ortho (coplanar) polychlorinated biphenyls (cPCBs) were performed on 35 ... [more ▼]

Congener-specific analyses of 7 polychlorinated dibenzo-p-dioxins (PCDDs), 10 polychlorinated dibenzofurans (PCDFs) and 4 non-ortho (coplanar) polychlorinated biphenyls (cPCBs) were performed on 35 samples of commercial long-life pasteurised cows' milk issued from eight different brands available in Walloon supermarkets (Belgium). The observed congener profile was characteristic of milk samples issued from industrialised countries with good inter and intra-brand reproducibility's. The PCDDs to PCDFs ratio was equal to 1.8 in concentration. The toxic equivalent (TEQ based on WHO-TEF) value for PCDD/Fs in all analysed milks was 1.09+/-0.30 pg TEQ/g fat (range 0.86-1.59), which is below the recommended EU non-commercialisation threshold value of 3 pg TEQ PCDD/Fs/g of milk fat. The mean TEQ value including cPCBs was 2.23+/-0.55 pg TEQ/g fat. These PCBs actually contributed for 49+/-8.6% of the total TEQ. Among PCDD/Fs and cPCBs, tetrachloro dibenzo-p-dioxin (TCDD), pentachloro dibenzo-p-dioxin (PeCDD), pentachloro dibenzofurans (PeCDFs) and 3,3',4,4',5-pentachloro biphenyl (PCB-126) were the most important contributors to the TEQ. Estimated daily intake (EDI) due to consumption of such milks was 0.34 pg TEQ/kg of body weight/day for PCDD/Fs and 0.69 pg TEQ/kg of body weight/day when cPCBs were included. [less ▲]

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See detailNew strategy for comprehensive analysis of polybrominated diphenyl ethers, polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and polychlorinated biphenyls by gas chromatography coupled with mass spectrometry
Pirard, Catherine; De Pauw, Edwin ULg; Focant, Jean-François ULg

in Journal of Chromatography. A (2003), 998(1-2), 169-181

A strategy for determination of polybrominated diphenyl ethers, polychlorinated dibenzodioxins, polychlorinated dibenzofurans and polychlorinated biphenyls on fatty matrices has been established. After ... [more ▼]

A strategy for determination of polybrominated diphenyl ethers, polychlorinated dibenzodioxins, polychlorinated dibenzofurans and polychlorinated biphenyls on fatty matrices has been established. After extraction, the proposed method allows the purification and the fractionation of all target groups of compounds in a simple multi-step automated clean-up. Furthermore, their subsequent analysis is carried out using a single benchtop mass spectrometer, in four separate injections. Required sensitivity considering levels found in the environment is attained using electron impact ionisation followed by tandem in time mass spectrometry. The whole method has been evaluated on standard solution and quality control samples consisting of fortified beef fat. Sensitivity, selectivity, accuracy and repeatability were tested with satisfactory results. (C) 2003 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailMeasurable impact of an old MSWI on the level of dioxins in free-range chickens and eggs grown in its vicinity
Pirard, C.; Focant, Jean-François ULg; Massart, A. C. et al

in Organohalogen Compounds (2003), 60-65

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See detailLevels of selected PBDEs and PCBs in Belgian human milk
Pirard, C.; De Pauw, Edwin ULg; Focant, Jean-François ULg

in Organohalogen Compounds (2003), 61

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See detailIncreased dioxin/PCB body burden in diabetics: findings in a population-based study in Belgium
Fierens, S.; Mairesse, H.; Heilier, H. et al

in Organohalogen Compounds (2003), 60-65

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See detailLevels of PCDDs, PCDFs and PCBs in International Fast Food Samples
Focant, Jean-François ULg; Pirard, Catherine; De Pauw, Edwin ULg

in Organohalogen Compounds (2003), 60-65

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See detailInteractions of cryptolepine and neocryptolepine with unusual DNA structures
Guittat, Lionel; Alberti, Patrizia; Rosu, Frédéric ULg et al

in Biochimie (2003), 85(5), 535-547

Cryptolepine, the main alkaloid present in the roots of Cryptolepis sanguinolenta, presents a large spectrum of biological properties. It has been reported to behave like a DNA intercalator with a ... [more ▼]

Cryptolepine, the main alkaloid present in the roots of Cryptolepis sanguinolenta, presents a large spectrum of biological properties. It has been reported to behave like a DNA intercalator with a preference for GC-rich sequences. In this study, dialysis competition assay and mass spectrometry experiments were used to determine the affinity of cryptolepine and neocryptolepine for DNA structures among duplexes, triplexes, quadruplexes and single strands. Our data confirm that cryptolepine and neocryptolepine prefer GC over AT-rich duplex sequences, but also recognize triplex and quadruplex structures. These compounds are weak telomerase inhibitors and exhibit a significant preference for triplexes over quadruplexes or duplexes. (C) 2003 Editions scientifiques et medicales Elsevier SAS and Societe francaise de biochimie et biologic moleculaire. All rights reserved. [less ▲]

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See detailInfluence of response factors on determining equilibrium association constants of non-covalent complexes by electrospray ionization mass spectrometry
Gabelica, Valérie ULg; Galic, Nives; Rosu, Frédéric ULg et al

in Journal of Mass Spectrometry [=JMS] (2003), 38(5), 491-501

A method for determining the equilibrium association constant of a complexation reaction A + B <=> AB by electrospray ionization mass spectrometry is described. The method consists in measuring the ... [more ▼]

A method for determining the equilibrium association constant of a complexation reaction A + B <=> AB by electrospray ionization mass spectrometry is described. The method consists in measuring the relative intensities of the peaks corresponding to A and to AB in equimolar A–B solutions at different concentrations C0. The results are fitted by a non-linear least-squares procedure, with the two variable parameters being the equilibrium association constant Ka and a factor R, defined by I(AB)/I.A/ = R× [AB]/[A]. The factor R is the ratio between the response factors of AB and A, and corrects for the relative electrospray responses of the complex and the free substrate A, mass discrimination of instrumental origin and/or moderate in-source dissociation. The method is illustrated with the following two systems: complexes between a double-stranded 12-base pair oligonucleotide and minor groove binders, and cyclodextrin complexeswith a,!-dicarboxylic acids. For the oligonucleotide complexes, it is found that the response of the complex is not dramatically different to the response of the free oligonucleotide duplex, as the double helix conformation is disturbed by the drug only to a minor extent. In the case of cyclodextrin complexes, these complexes were found to have a much higher response than free cyclodextrin. This may be due to the fact that cyclodextrin is neutral in solution, whereas the complex is charged, but it can also stem from the fact that a significant proportion of the complex is in a non-inclusion geometry. The present method requires the exact determination of the concentrations of the reactants and is applicable to 1 : 1 complexes. [less ▲]

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See detailValidation and discussion of CALUX analysis for marine samples
Windal, Isabelle; Van Wouwe, Nathalie; Carbonnelle, Sophie et al

in Organohalogen Compounds (2003)

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See detailSelective adsorption of dioxins and PCBs from marine oils on activated carbon
De Meulenaer, Bruno; Maes, Jeroen; Van Heerswynghels, Peter et al

in Organohalogen Compounds (2003)

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See detailAnalysis of PCDD/Fs in human blood plasma using CALUX bioassay and GC-HRMS: a comparison
Van Wouwe, Nathalie; Eppe, Gauthier ULg; Xhrouet, Céline ULg et al

in Organohalogen Compounds (2003)

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See detailDioxins, furans and dioxin-like PCBs in juvenile harbour porpoises (Phocoena Phocoena) from the North sea
Beans, Cristina; Debacker, Virginie ULg; Jauniaux, Thierry ULg et al

in Organohalogen Compounds (2003)

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See detailThermometer ions for matrix-enhanced laser desorption/ionization internal energy calibration
Greisch, Jean-François ULg; Gabelica, Valérie ULg; Remacle, Françoise ULg et al

in Rapid Communications in Mass Spectrometry : RCM (2003), 17(16), 1847-1854

This work describes a method to use relative fragmentation yields to characterize the internal energy distribution of ions produced by matrix-enhanced laser desorption/ionization mass spectrometry (MELDI ... [more ▼]

This work describes a method to use relative fragmentation yields to characterize the internal energy distribution of ions produced by matrix-enhanced laser desorption/ionization mass spectrometry (MELDI-MS, see: Wright LG, Cooks RG, Wood KL. Biomed. Mass Spectrom. 1985; 12:153162). Assuming that the fragmentation proceeds statistically and that the collisions in the source lead to a Boltzmann-like distribution of the internal energy, a characteristic parameter, the effective temperature, is introduced to describe the internal energy distribution of the ions observed. The hypotheses, advantages and drawbacks of the implementation of the method that uses substituted benzylpyridinium salts as thermometer ions are discussed. Use is made of two matrices that produce no matrix cations in MELDI and are suitable for small cationic salts. The actual value of this effective temperature significantly depends on an accurate determination of the threshold dissociation energies and on the time spent in the source, in addition to the statistical hypothesis itself. The method could be applied to normalize spectra in order to compare results issued from different instruments. [less ▲]

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