References of "De Pauw, Edwin"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailCovalent binding of antitumor benzoacronycines to double-stranded DNA induces helix opening and the formation of single-stranded DNA: Unique consequences of a novel DNA-bonding mechanism
David-Cordonnier, MH; Laine, W; Lansiaux, A et al

in Molecular Cancer Therapeutics (2005), 4(1), 71-80

The majority of DNA-binding small molecules known thus far stabilize duplex DNA against heat denaturation. A high, drug-induced increase in the melting temperature (T-m) of DNA is generally viewed as a ... [more ▼]

The majority of DNA-binding small molecules known thus far stabilize duplex DNA against heat denaturation. A high, drug-induced increase in the melting temperature (T-m) of DNA is generally viewed as a good criterion to select DNA ligands and is a common feature of several anticancer drugs such as intercalators (e.g., anthracyclines) and alkylators (e.g., ecteinascidin 743). The reverse situation (destabilization of DNA to facilitate its denaturation) may be an attractive option for the identification of therapeutic agents acting on the DNA structure. We have identified the tumor-active benzoacronycine derivative S23906-1 [(+/-)-cis-1, 2-diacetoxy-6-methoxy-3,3,14-trimethyl 1,2,3,14-tetrahydro-7H-benzo[b]pyrano[3,2]acridin-7- one] as a potent DNA alkylating agent endowed with a helicase-like activity. Using complementary molecular approaches, we show that covalent binding to DNA of the diacetate compound S23906-1 and its monoacetate analogue S28687-1 induces a marked destabilization of the double helix with the formation of alkylated ssDNA. The DNA-bonding properties and effects on DNA structure of a series of benzoacronycine derivatives, including the dicarbamate analogue S29385-1, were studied using complementary biochemical (electromobility shift assay, nuclease S1 mapping) and spectroscopic (fluorescence and T-m measurements) approaches. Alkylation of guanines in DNA by S28687-1 leads to a local denaturation of DNA, which becomes susceptible to cleavage by nuclease S1 and significantly decreases the T-m of DNA. The drug also directly alkylates single-strand DNA, but mass spectrometry experiments indicate that guanines in duplexes are largely preferred over single-stranded structures. This molecular study expands the repertoire of DNA-binding mechanisms and provides a new dimension for DNA recognition by small molecules. [less ▲]

Detailed reference viewed: 41 (13 ULg)
Full Text
Peer Reviewed
See detailAphid adaptation to plant defence system
Francis, Frédéric ULg; Harmel, Nicolas ULg; Gerkens, Pascal et al

in Abstract book (2005)

Detailed reference viewed: 26 (3 ULg)
Full Text
Peer Reviewed
See detailAscididemin and meridine stabilise G-quadruplexes and inhibit telomerase in vitro
Guittat, Lionel; De Cian, Anne; Rosu, Frédéric ULg et al

in Biochimica et Biophysica Acta - General Subjects (2005), 1724(3), 375-384

Ascididemin and Meridine are two marine compounds with pyridoacridine skeletons known to exhibit interesting antitumour activities. These molecules have been reported to behave like DNA intercalators. In ... [more ▼]

Ascididemin and Meridine are two marine compounds with pyridoacridine skeletons known to exhibit interesting antitumour activities. These molecules have been reported to behave like DNA intercalators. In this study, dialysis competition assay and mass spectrometry experiments were used to determine the affinity of ascididemin and meridine for DNA structures among duplexes, triplexes, quadruplexes and single-strands. Our data confirm that ascididemin and meridine interact with DNA but also recognize triplex and quadruplex structures. These molecules exhibit a significant preference for quadruplexes over duplexes or single-strands. Meridine is a stronger quadruplex ligand and therefore a stronger telomerase inhibitor than ascididemin (IC50= 11 and > 80 mu M, respectively in a standard TRAP assay). (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 77 (2 ULg)
Full Text
Peer Reviewed
See detailProteomic study of plant-aphid interactions: between defense and adaptations.
Francis, Frédéric ULg; Gerkens, P.; De Pauw, Edwin ULg et al

in Comparative Biochemistry and Physiology. Part A, Physiology (2005), 141

Detailed reference viewed: 25 (6 ULg)
Full Text
Peer Reviewed
See detailIdentification of Potential Biomarkers of Human Peripheral Blood Mononuclear Cell intoxication by dioxins
Brenez, Cécile; Cellier, Nicolas; Gerkens, Pascal et al

in Organohalogen Compounds (2005), 67

Detailed reference viewed: 16 (1 ULg)
Full Text
Peer Reviewed
See detailRemoval of PCDD/Fs and DL-PCBs from fish oil by activated carbon: Compliance with European Legislation
Eppe, Gauthier ULg; Carbonnelle, Sophie; Hellebosch, Laeticia et al

in Organohalogen Compounds (2005), 67

Detailed reference viewed: 14 (1 ULg)
Full Text
Peer Reviewed
See detailDecrease of serum concentrations of dioxins and PCBs in Belgium between 2000 and 2003
Fierens, Sébastien; Heilier, Jean-François; Eppe, Gauthier ULg et al

in Organohalogen Compounds (2005), 67

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailFast gas-phase hydrogen/deuterium exchange observed for a DNA G-quadruplex
Gabelica, Valérie ULg; Rosu, Frédéric ULg; Witt, Matthias et al

in Rapid Communications in Mass Spectrometry : RCM (2005), 19(2), 201-208

The gas-phase hydrogen/deuterium (H/D) exchange kinetics of DNA G-quadruplexes has been investigated using Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS). The quadruplex [(TGGGGT)(4 ... [more ▼]

The gas-phase hydrogen/deuterium (H/D) exchange kinetics of DNA G-quadruplexes has been investigated using Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS). The quadruplex [(TGGGGT)(4).3NH(4)(+)] undergoes very fast H/D exchange, in both the positive and in the negative ion modes, compared to DNA duplexes and other quadruplexes tested, and compared to the corresponding single-stranded TGGGGT. Substitution of NH4+ for K+ did not alter this fast H/D exchange, indicating that the hydrogens of the ammonium ions are not those exchanged. However, stripping of the interior cations of the quadruplex by source collision-induced dissociation (CID) in the positive ion mode showed that the presence of the inner cations is essential for the fast exchange to be possible. Molecular dynamics simulations show that the G-quadruplex is very rigid in the gas phase with NH4+ ions inside the tetrads. We suggest that the fast H/D exchange is favored by this rigid quadruplex conformation. This example illustrates that the concept that compact DNA structures exchange H for D slower than unfolded ones is a misconception. Copyright (C) 2004 John Wiley Sons, Ltd. [less ▲]

Detailed reference viewed: 42 (3 ULg)
Full Text
Peer Reviewed
See detailInternal energy and fragmentation of ions produced in electrospray sources
Gabelica, Valérie ULg; De Pauw, Edwin ULg

in Mass Spectrometry Reviews (2005), 24(4), 566-587

This review addresses the determination of the internal energy of ions produced by electrospray ionization (ESI) sources, and the influence of the internal energy on analyte fragmentation. A control of ... [more ▼]

This review addresses the determination of the internal energy of ions produced by electrospray ionization (ESI) sources, and the influence of the internal energy on analyte fragmentation. A control of the analyte internal energy is crucial for several applications of electrospray mass spectrometry, like structural studies, construction of reproducible and exportable spectral libraries, analysis of non-covalent complexes. Sections II and III summarize the Electrospray mechanisms and source design considerations which are relevant to the problem of internal energy, and Section IV gives an overview of the interrelationships between ion internal energy, reaction time scale, and analyte fragmentation. In these three sections we tried to make the most important theoretical elements understandable by all ESI users, and their understanding requires a minimal background in physical chemistry. We then present the different approaches used to experimentally determine the ion internal energy, as well as various attempts in modeling the internal energy uptake in electrospray sources. Finally, a tentative comparison between electrospray and other ionization sources is made. As the reader will see, although many reports appeared on the subject, the knowledge in the field of internal energy of ions produced by soft ionization sources is still scarce, because of the complexity of the system, and this is what makes this area of research so interesting. The last section presents some perspectives for future research. [less ▲]

Detailed reference viewed: 69 (14 ULg)
Full Text
Peer Reviewed
See detailSteatosis-induced proteomic changes in liver mitochondria evidenced by two-dimensional differential in-gel electrophoresis
Douette, Pierre ULg; Navet, Rachel ULg; Gerkens, Pascal et al

in Journal of Proteome Research (2005), 4(6), 2024-2031

Steatosis encompasses the accumulation of droplets of fats into hepatocytes. In this work, we performed a comparative analysis of mitochondrial protein patterns found in wild-type and steatosis-affected ... [more ▼]

Steatosis encompasses the accumulation of droplets of fats into hepatocytes. In this work, we performed a comparative analysis of mitochondrial protein patterns found in wild-type and steatosis-affected liver using the novel technique two-dimensional differential in-gel electrophoresis (2D-DIGE). A total of 56 proteins exhibiting significant difference in their abundances were unambiguously identified. Interestingly, major proteins that regulate generation and consumption of the acetyl-CoA pool were dramatically changed during steatosis. Many proteins involved in the response to oxidative stress were also affected. [less ▲]

Detailed reference viewed: 34 (6 ULg)
Full Text
Peer Reviewed
See detailProteomic approach to investigate plant – aphid interactions
Francis, Frédéric ULg; Gerkens, Pascal; De Pauw, Edwin ULg et al

Conference (2005)

Detailed reference viewed: 13 (5 ULg)
Full Text
Peer Reviewed
See detailFactors influencing the PCDD/F levels in plasma of Belgian blood donors
Debacker, Neomi; Van Wouwe, Nathalie; Sasse, André et al

in Organohalogen Compounds (2005)

Detailed reference viewed: 16 (0 ULg)