References of "Daube, Georges"
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See detailTraçabilité dans la filière viande. I. La traçabilité administrative.
Evrard, V.; China, B.; Noirfalise, R. et al

in Annales de Médecine Vétérinaire (2001), 145

Le secteur de la viande a été secoué ces dernières années par quelques scandales, tels ceux des hormones et de la dioxine, avec pour conséquences une perte de confiance de la part du consommateur et une ... [more ▼]

Le secteur de la viande a été secoué ces dernières années par quelques scandales, tels ceux des hormones et de la dioxine, avec pour conséquences une perte de confiance de la part du consommateur et une perturbation du marché de la viande. Pour redresser l’image des produits carnés belges, il est important de pouvoir en déterminer et en garantir l’origine. En Belgique, il existe divers systèmes de traçabilité administrative dont le principal est le système SANITEL qui comprend un système automatisé de traitement de données relatives à l’identification et l’enregistrement des animaux. Au-delà de l’aspect légal et réglementaire, différentes initiatives, visant une amélioration de la qualité, fleurissent : "les labels". Ceux-ci intègrent fréquemment la traçabilité dans leur cahier des charges. La traçabilité administrative n’est pas infaillible, la perte de documents et les fraudes peuvent ternir l’image de celle-ci. C’est pourquoi le système documentaire a été associé aux empreintes génétiques des animaux. 5 [less ▲]

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See detailL'entérotoxémie bovine en Belgique. II. Epizootiologie élémentaire et pathologie descriptive.
Manteca, Christophe; Daube, Georges ULg; Jauniaux, Thierry ULg et al

in Annales de Médecine Vétérinaire (2000), 145

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See detailLa contamination de l'eau et des aliments par les virus pathogènes pour l'homme
Scipioni, Alexandra ULg; Daube, Georges ULg; Thiry, Etienne ULg

in Annales de Médecine Vétérinaire (2000), 144

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See detailLa maîtrise des risques microbiologiques liés à la viande fraîche en Belgique
Daube, Georges ULg

Article for general public (2000)

Nous connaissons de mieux en mieux les risques liés à la consommation de viande fraîche. Certains risques biologiques diminuent comme la yersiniose ou la brucellose ; certains semblent augmenter, telles ... [more ▼]

Nous connaissons de mieux en mieux les risques liés à la consommation de viande fraîche. Certains risques biologiques diminuent comme la yersiniose ou la brucellose ; certains semblent augmenter, telles la salmonellose ou la campylobactériose ; enfin, certains émergent, comme les syndromes hémolytiques et urémiques dus à E. coli, ou ont été récemment identifiés comme transmissibles par les aliments, comme la cryptosporidiose et la listériose. Ce tableau un peu sombre et les solutions qui semblent bien complexes à mettre en œuvre ne doit pas nous faire oublier que pratiquement tous ces agents microbiens sont détruits par une cuisson à cœur de quelques minutes au delà de 70°C, barème qui correspond à l’arrêt d’écoulement de liquide sanguinolent. Il faut, en outre, éviter les contaminations croisées entre les viandes crues et les autres aliments via les mains ou les équipements de préparation des aliments. De bonnes pratiques d’hygiène culinaire au stade du consommateur peuvent donc réduire le risque biologique à un niveau très bas. Cette alternative devrait donner le temps à nos autorités et à nos entreprises de réduire drastiquement les risques jugés les moins tolérables par des actions ciblées tout au long de la filière de production des viandes. Cependant, il faudra toujours considérer la consommation de viande crue, comme un comportement à risque. Le tout est de le maintenir à un niveau compatible avec les possibilités de notre société et d'informer les populations les plus à risque. La nouvelle agence fédérale pour la sécurité de la chaîne alimentaire aura cette mission à remplir. [less ▲]

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See detailAnalysis of foodborne disease in Belgium in 1997.
Van Loock, F.; Ducoffre, G.; Dumont, J.-M. et al

in Acta Clinica Belgica (2000), 55

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See detailEtude du rôle de Clostridium perfringens dans l'entérotoxémie bovine
Manteca, Christophe; Daube, Georges ULg; Mainil, Jacques ULg

Conference (1999)

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See detailEtude du role de Clostridium perfringens dans l'entérotoxémie bovine
Manteca, Christophe; Daube, Georges ULg; Mainil, Jacques ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1999), 154(6, Pt 2),

Bovine enterotoxaemia is an acute to peracute syndrome occurring mainly in calves and characterized by the sudden or very rapid death of the calf, with colics, convulsions and nervous disorders as ... [more ▼]

Bovine enterotoxaemia is an acute to peracute syndrome occurring mainly in calves and characterized by the sudden or very rapid death of the calf, with colics, convulsions and nervous disorders as clinical signs, if any. The most pronounced lesion is a necrohaemorrhagic enteritis of the jejunum, the ileum, and sometimes the colon. Suckling beef calves are the most frequently affected ones. In 67% of the 78 field cases investigated, some kind of stress was observed 24 to 36 hours prior to the death: change in diet or pasture, vaccination... The most frequently isolated bacteria, and the one isolated in highest numbers, was non-sporulated non-enterotoxigenic toxinotype A Clostridium perfringens. Reproduction of the lesions was successful in a ligated intestinal loop assay in one calf with a few of these strains, more especially with one of them, which was shown later to produce another recently described toxin, the beta 2 toxin. A role for this beta 2 toxin in bovine enterotoxaemia is thus speculated for future research. [less ▲]

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See detailVirulence Plasmids of Enterotoxigenic Escherichia Coli Isolates from Piglets
Mainil, Jacques ULg; Daube, Georges ULg; Jacquemin, Etienne et al

in Veterinary Microbiology (1998), 62(4), 291-301

Virulence plasmids of 68 ETEC isolates from piglets belonging to different pathotypes and six ETEC isolates from calves with pathotypes typical of porcine ETEC were identified with seven virulence probes ... [more ▼]

Virulence plasmids of 68 ETEC isolates from piglets belonging to different pathotypes and six ETEC isolates from calves with pathotypes typical of porcine ETEC were identified with seven virulence probes for the heat-stable (STa and STb) and heat-labile (LT) enterotoxins, for the F4, F5, F6, and F41 fimbrial adhesion subunit, and also with five Rep probes for the RepFIA and RepFIB basic replicons, and the RepFIC family of basic replicons. With the exception of the F41 probe, the other virulence probes hybridized with at least one plasmid band of a size range from 65 to more than 100 Mda. Common associations of virulence factor-encoding genes on plasmid bands were: STb/LT, STa/F5, STa/F6, STa/STb. Other associations, STa/F4, STa/F4/F6, and STa/STb/LT/F6, were rarer. On the other hand the F4 adhesin-encoding genes were isolated on one plasmid band in all but three F4+ isolates. All but one of the 92 virulence plasmids which were studied have Rep probe hybridization profiles and replicon types typical of the uni- or multireplicon plasmids belonging to the various incompatibility groups of the F incompatibility complex. [less ▲]

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See detailAn Efficient Sampling Technique Used to Detect Four Foodborne Pathogens on Pork and Beef Carcasses in Nine Belgian Abattoirs
Korsak Koulagenko, Nicolas ULg; Daube, Georges ULg; Ghafir, Yasmine et al

in Journal of Food Protection (1998), 61(5), 535-541

The method presented in this paper should prove useful in assessing the effectiveness of HACCP plans developed in slaughterhouses. Samples were collected by swabbing well-defined areas of pork and beef ... [more ▼]

The method presented in this paper should prove useful in assessing the effectiveness of HACCP plans developed in slaughterhouses. Samples were collected by swabbing well-defined areas of pork and beef carcasses with sterile gauze. Between 160 and 420 half-carcasses were swabbed in each of nine pork or beef slaughterhouses. Swabs from five carcasses were placed in the same sterile Stomacher bag, constituting a single composite sample. Standard or validated analytical methods were used to isolate and characterize four foodborne pathogens. Salmonella spp., Listeria monocytogenes, Campylobacter spp., and verocytotoxin-producing E. coli were detected, respectively, in 27, 2, 2, and 14% of the pork samples and 0, 22, 10, and 5% of the beef samples. Of the 10 samples positive for E. coli O157, only one yielded an isolate confirmed to be enterohemorrhagic. Since Salmonella spp. appear as the main contaminant port (27%) and L. monocytogenes as the main containment of beef (22%), any slaughterhouse sampling plan should include testing for the former in the case of pork carcasses and for the latter in the case of beef carcasses. One should also test regularly for the presence of E. coli O157 and Campylobacter spp. in pork and beef abattoirs. The method presented here is an easy way to assess the contamination rate of carcasses at the end of the slaughtering process. [less ▲]

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See detailClostridium perfringens urease genes are plasmid-borne.
Dupuy, B.; Daube, Georges ULg; Popoff, M. R. et al

in Infection and Immunity (1997), 65

Although many bacteria are ureolytic, and in some cases urease acts as a virulence factor, the urease phenotype has not been analyzed in the anaerobic pathogen Clostridium perfringens. In this study, ;2 ... [more ▼]

Although many bacteria are ureolytic, and in some cases urease acts as a virulence factor, the urease phenotype has not been analyzed in the anaerobic pathogen Clostridium perfringens. In this study, ;2% of C. perfringens strains, representing the principal biotypes, were found to harbor the urease structural genes, ureABC, and these were localized on large plasmids that often encode, in addition, the lethal « or i toxins or the enterotoxin. This represents the first report of a plasmid-encoded urease in a gram-positive bacterium. The C. perfringens enzyme was highly similar to the ureases of other bacteria and cross-reacted with antibodies raised against the urease purified from Helicobacter pylori. Urease production was inhibited by urea and induced under growth conditions where the availability of nitrogen sources was limiting. To date, this form of regulation has been observed only for chromosomal ureABC genes. [less ▲]

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See detailSurveillance of foodborne illnesses in Belgium.
Daube, Georges ULg; Van Look, F.

in Archives of Public Health (1997), 55

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See detailIsolation, identification and serotyping of Clostridium perfringens from goats.
Phukan, A.; Dutta, G. N.; Daube, Georges ULg et al

in Indian Journal of Animal Sciences (The) (1997), 67

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See detailIsolation of O157 : H7 and other enterohaemorrhagic Escherichia coli from foodstuffs.
Daube, Georges ULg; Chahed, Amina

in Factors affecting the microbial quality of meat, 4. Microbial methods for the meat industry. Concerted Action CT94-1456 (1997)

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See detailCharacterization of Clostridium perfringens isolated from necrotic enteritis of fowls.
Das, B. C.; Dutta, G. N.; Daube, Georges ULg et al

in Indian Journal of Animal Sciences (The) (1997), 67

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See detailCharacterization of Clostridium perfringens isolates from goats.
Phukan, A.; Dutta, G.-N.; Daube, Georges ULg et al

in Indian Veterinary Journal (The) (1997), 74

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See detailClostridium spiroforme toxin genes are related to C. perfringens iota toxin genes but have a different genomic localization.
Gilbert, M.; Perelle, S.; Daube, Georges ULg et al

in Systematic & Applied Microbiology (1997), 20

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See detailL'entérotoxémie bovine: rôle de Clostridium perfringens
Daube, Georges ULg; Ginter, A; Manteca, C et al

Book published by Ministère de l'Agriculture et des Classes Moyennes (1996)

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See detailHybridization of 2,659 Clostridium Perfringens Isolates with Gene Probes for Seven Toxins (Alpha, Beta, Epsilon, Iota, Theta, Mu, and Enterotoxin) and for Sialidase
Daube, Georges ULg; Simon, Patricia ULg; Limbourg, Bernard et al

in American Journal of Veterinary Research (1996), 57(4), 496-501

OBJECTIVE--To genetically characterize Clostridium perfringens isolates for association of pathologic type with various diseases. DESIGN--Prospective study. SAMPLE POPULATION--2,659 C perfringens isolates ... [more ▼]

OBJECTIVE--To genetically characterize Clostridium perfringens isolates for association of pathologic type with various diseases. DESIGN--Prospective study. SAMPLE POPULATION--2,659 C perfringens isolates from various nonhuman animals species, human beings, and foods. PROCEDURE--Colony hybridization with DNA probes for 7 toxin (alpha, beta, epsilon, iota (subunits a and b), theta, mu, and enterotoxin) genes and 1 sialidase gene were performed to group the isolates by pathologic type. RESULTS--Enterotoxin-negative type-A isolates were the most common (2,575/2,659), were isolated from all sources, and were separated into 5 pathologic types. In cattle and horses with enterotoxemia, essentially only these pathologic types were identified. The enterotoxin-negative isolates of types C or D each had a single pathologic type. Type-C isolates were isolated only from swine with necrotic enteritis and type-D isolates from small ruminants with enterotoxemia, except that 1 type-D isolate was also found from a healthy fish. Type-B or -E isolates were not found. Among the 47 enterotoxin-positive isolates, 5 isolates from sheep or deer were type D and the other 42 were type A. These 42 isolates were grouped into 3 pathologic types: 1 type was isolated from samples of almost all origins, but the other 2 types were found in only 5 fish, 4 human beings, and 1 dog. CONCLUSIONS AND CLINICAL RELEVANCE--Genetic characterization of these isolates allowed identification of 11 different pathologic types. This approach may be useful in molecular diagnosis and prophylaxis of clostridial disease. [less ▲]

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See detailVers une assurance qualité intégrée de la chaîne agro-alimentaire.
Vindevogel, Henri ULg; Daube, Georges ULg; Hans, J. C. et al

in Annales de Médecine Vétérinaire (1996), 140

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See detailDéveloppement actuel du concept de l'hygiène en agro-alimentaire et des exigences des normes européennes.
Vindevogel, Henri ULg; Daube, Georges ULg; Hans, J. C. et al

in Annales de Médecine Vétérinaire (1996), 140

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