References of "Daube, Georges"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailShellfish, a dangerous food?
China, Bernard; de Schaetzen, Marie-Athénaïs ULg; Daube, Georges ULg

in Annales de Médecine Vétérinaire (2003), 147(6, DEC-JAN), 413-422

Sea products are consumed worldwide. Seafood, especially shellfish, is consumed crude or not well cooked therefore they are risky for the development of foodborne diseases. Moreover, these animals filter ... [more ▼]

Sea products are consumed worldwide. Seafood, especially shellfish, is consumed crude or not well cooked therefore they are risky for the development of foodborne diseases. Moreover, these animals filter water and concentrate the microorganisms and the toxins. Risks are multiple : bacteria (Clostridium, Vibrio), viruses (norovirus, hepatitis A) and the biotoxins (paralytic, neurotoxic, diarrhoeic and amnesic) produced by phytoplankton. The European regulation 91/492/EC indicates the microbiological and toxicological criteria for the sale of the shellfish. Nevertheless, in the absence of good diagnosis methods, the viruses were omitted in this regulation. But it is a major cause of toxi-infection due to shellfish consumption. The large number of outbreaks worldwide due to the ingestion of seafood indicates that these ones must be carefully controlled. Therefore, we need powerful diagnosis tools. The aim of this review is to describe the major biological risks (bacteria, viruses, biotoxins) for the human health linked to the consumption of sea food. [less ▲]

Detailed reference viewed: 127 (8 ULg)
See detailMicrobiologische risico's in verbans met voeding
Daube, Georges ULg

in Institut Danone (Ed.) Boesdselveliligheid : een wetenschapelijke benadering (2003)

Detailed reference viewed: 26 (0 ULg)
See detailLe risque microbiologique lié à l'ailimentation
Daube, Georges ULg

in Institut Danone (Ed.) La sécurité alimentaire : une approche scientifique (2003)

Detailed reference viewed: 34 (2 ULg)
Full Text
Peer Reviewed
See detailInfluence of a water purification unit on the contamination level of salmonella in outcoming water and sludge
Jacob, Benoit; Korsak Koulagenko, Nicolas ULg; Grooven, Bénédicte et al

in Annales de Médecine Vétérinaire (2002), 146(5), 303-310

Foodborne pathogens occasionally harboured in the gastro-intestinal tract of some domestic animals may be retrieved in slaughterhouses waste water and in sludge of water purification units. Salmonella ... [more ▼]

Foodborne pathogens occasionally harboured in the gastro-intestinal tract of some domestic animals may be retrieved in slaughterhouses waste water and in sludge of water purification units. Salmonella, athogen common to man and Animals, is often used as a biological risk indicator. The aim of the present study was to assess effectiveness of a recent water purification unit by rapid and semi-quantitative detection of this micro-organism. The water purification unit collects waste water of seven food-processing industries, including a pig slaughterhouse. This latest was the main source of Salmonella contamination with a level of more 103 colony forming unit (CFU) per ml, that was very close to the average level of contamination of total incoming waste water. The unit, whose principle of action is based on biological purification, has permitted a 4 log10 reduction of Salmonella contamination, with a final contamination level of less than 1 CFU/ml. It was observed only a little decrease of contamination levels during the biological treatment steps, in contrast with the one observed during the clarification step. This was due to adsorption of bacteria by material in suspension. Fresh sludge were harboured an average of 102 CFU of Salmonella per gram. In the beginning of December 1999, two sludge piles were let on the ground along a field and sampled microbiologically every month. Seven month later, no Salmonella were recovered from the piles. 309 [less ▲]

Detailed reference viewed: 95 (10 ULg)
Full Text
Peer Reviewed
See detailEcology of Salmonella in slaughter pigs digestive tract and study of the contamination of carcasses
Boudry, Christelle ULg; Korsak Koulagenko, Nicolas ULg; Jacob, Benoit et al

in Annales de Médecine Vétérinaire (2002), 146(6, DEC-JAN), 353-360

This article presents a study on the Salmonella asymptomatic carriage of finishing pigs and its consequences on the contamination of carcasses. Twenty finishing pigs were followed to determine the faecal ... [more ▼]

This article presents a study on the Salmonella asymptomatic carriage of finishing pigs and its consequences on the contamination of carcasses. Twenty finishing pigs were followed to determine the faecal shedding of Salmonella. No sample was positive but at the slaughterhouse, the analysis of tonsils and certain parts of the digestive tract (mesenterial lymph nodes, ileum and large intestine content) revealed that 70% of the pigs were positive. The strains isolated from the faecal samples of the large intestine and from tonsils were identical, which might indicate that there has been an oral contamination from pig to pig due to the shedding of Salmonella or a carriage of different strains in different organs. The mesenterial lymph nodes (40 % positive) were contaminated by strains which were different from those from the faecal samples and tonsils. Salmonella strains were only found on the swabs of one carcass. A complementary assay has shown the negative role played by the scalding step. This latter may contaminate the oral and rectal cavities of slaughtered animals. This entire study reveals the difficulty to predict the contamination risk at the slaughterhouse from the analysis of finishing pigs faecal samples. Therefore, a better knowledge of the carriage and the shedding of Salmonella during the finishing period and during the slaughter is necessary to minimise meat contamination. [less ▲]

Detailed reference viewed: 193 (51 ULg)
Full Text
Peer Reviewed
See detailQuantitative and qualitative estimation of bacteria in foodstuffs by genetic amplification
China, Bernard; Ghafir, Yasmine; Daube, Georges ULg

in Annales de Médecine Vétérinaire (2002), 146(2, APR-MAY),

Food quality is a major concern of our modern society. The sudden awareness that food is not sterile underlines the need of a rigorous microbiological analysis of foodstuffs. The techniques developed ... [more ▼]

Food quality is a major concern of our modern society. The sudden awareness that food is not sterile underlines the need of a rigorous microbiological analysis of foodstuffs. The techniques developed until now require the growth of the microorganism on a selective medium allowing to differentiate it from other bacteria. This procedure could be long and difficult for the analysis. To improve these classical methods, molecular biology techniques based on the specific amplification of a DNA sequence are in development. Moreover, the apparition of real time PCR allows new future prospects since it allows to quantify the number of bacteria present in a sample. This quantitative aspect is very important in food microbiology. In this way, the molecular biology tools should allow to develop fast, sensitive, specific and quantitative diagnosis tests to confirm the results obtained by classical methods but also to allow the diagnosis of fastidious bacteria. [less ▲]

Detailed reference viewed: 112 (1 ULg)
Full Text
Peer Reviewed
See detailProposal for a new standard of faecal contamination indicator: Bifidobacterium
Delcenserie, Véronique ULg; China, Bernard; Gavini, Françoise et al

in Annales de Médecine Vétérinaire (2002), 146(5, OCT-NOV), 279-293

Micro-organisms of the genus Bifidobacterium are some of the most common micro-organisms in the human and animal intestinal tract. Bifidobacterium species are well known for their beneficial effects on ... [more ▼]

Micro-organisms of the genus Bifidobacterium are some of the most common micro-organisms in the human and animal intestinal tract. Bifidobacterium species are well known for their beneficial effects on the microflora. In this article, the interest for this microorganism is other. If this microorganism is found at different stages of the processing line, it indicates often a contamination of this processing line. An other characteristic is that Bifidobacterium species are different according to the host. It could be possible to determine the contamination origin (human or animal). That is an advantage over other bacteria such as the actual faecal contamination indicator, Escherichia coli. The aim of this project is to develop a test, using molecular genetic tools, to rapidly detect the most important Bifidobacterium species in food. If we know the contamination sources, the critical points will be pointed out and the control measures will be better. [less ▲]

Detailed reference viewed: 123 (20 ULg)
See detailPrevalence of Salmonella along a meat pork production system.
Korsak Koulagenko, Nicolas ULg; Daube, Georges ULg

in Korsak Koulagenko, Nicolas; Groven, B.; Jacob, B. (Eds.) et al Food safety assurance and veterinary public health. Food safety assurance in the pre-harvest phase. (2002)

Detailed reference viewed: 75 (2 ULg)
Full Text
Peer Reviewed
See detailA role for the Clostridium perfringens beta 2 toxin in bovine enterotoxaemia?
Manteca, Christophe; Daube, Georges ULg; Jauniaux, Thierry ULg et al

in Veterinary Microbiology (2002), 86(3), 191-202

Non-enterotoxigenic type A Clostridium perfringens are associated with bovine enterotoxaemia, but the alpha toxin is not regarded as responsible for the production of typical lesions of necrotic and ... [more ▼]

Non-enterotoxigenic type A Clostridium perfringens are associated with bovine enterotoxaemia, but the alpha toxin is not regarded as responsible for the production of typical lesions of necrotic and haemorrhagic enteritis. The purpose of this study was to investigate the putative role of the more recently described beta2 toxin. Seven hundred and fourteen non-enterotoxigenic type A C. perfringens isolated from 133 calves with lesions of enterotoxaemia and high clostridial cell counts (study population) and 386 isolated from a control population of 87 calves were tested by a colony hybridisation assay for the beta2 toxin. Two hundred and eighteen (31%) C perfringens isolated from 83 calves (62%) of the study population and 113 (29%) C. perfringens isolated from 51 calves (59%) of the control population tested positive with the beta2 probe. Pure and mixed cultures of four C perfringens (one alpha+beta2+, one alpha+enterotoxin-1 and two alpha+) were tested in the ligated loop assay in one calf. Macroscopic haemorrhages of the intestinal wall, necrosis and haemorrhages of the intestinal content, and microscopic lesions of necrosis and polymorphonuclear and mononuclear cell infiltration of the intestinal villi were more pronounced in loops inoculated with the a and beta2-toxigenic C. perfringens isolate. These results suggest in vivo synergistic role of the alpha and beta2 toxins in the production of necrotic and haemorrhagic lesions of the small intestine in cases of bovine enterotoxaemia. However, isolation of beta2-toxigenic C. perfringens does not confirm the clinical diagnosis of bovine enterotoxaemia and a clostridial cell counts must still be performed. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

Detailed reference viewed: 132 (25 ULg)
Full Text
Peer Reviewed
See detailIsolation of Clostridium perfringens from three neonatal calves with haemorrhagic abomasitis.
Manteca, C.; Jauniaux, Thierry ULg; Daube, Georges ULg et al

in Revue de Médecine Vétérinaire (2001), 152

Braxy-like disease with sudden death and acute haemorhagic abomasitis was diagnosed in three Belgian Blue calves : one two-day-old and one one month-old calves, in good condition with no clinical signs ... [more ▼]

Braxy-like disease with sudden death and acute haemorhagic abomasitis was diagnosed in three Belgian Blue calves : one two-day-old and one one month-old calves, in good condition with no clinical signs noted a few hours prior to death, and another two day-old calf, which had shown problems of abomasal dilatation and regurgitation prior to death. Histologically, the abomasal wall were oedematous and emphysematous. A pure and abundant growth of Clostridium perfringens was obtained in anaerobic conditions from the abomasal wall of the three Belgian Blue calves. No bacterial growth was obtained in aerobic conditions. The calf with digestive disorders was also positive for BVD virus by immunofluorescence in the abomasal wall and in the spleen. [less ▲]

Detailed reference viewed: 110 (3 ULg)
Full Text
See detailHuman viral infections derived from food
Scipioni, Alexandra ULg; Daube, Georges ULg; Thiry, Etienne ULg

in Point Vétérinaire (2001), 32(218, AUG-SEP),

Several viruses may be transmitted to humans via food including hepatitis A and E virus (VHA and VHE), Norwalk type calicivirus, rotavirus and astrovirus. Enterovirus and enteric adenovirus may also be ... [more ▼]

Several viruses may be transmitted to humans via food including hepatitis A and E virus (VHA and VHE), Norwalk type calicivirus, rotavirus and astrovirus. Enterovirus and enteric adenovirus may also be transmitted. Sick people or healthy carriers excrete these viruses in their stools. Shellfish and water are the most frequently contaminated foodstuffs. Another route of infection is during handling of food. Detection of these viruses in food is complex for several reasons: the interaction of virus and food makes viral concentration and purification difficult, in vitro culture of these viruses is difficult if not impossible, and the quantity of virus present in the sample is low. Molecular technology (PCR) is the most suitable method of detection. Another method is to choose an indicator of viral faecal contamination. [less ▲]

Detailed reference viewed: 93 (6 ULg)
See detailDifferentiation of Bifidobacterium species by PCR-RFLP
Delcenserie, Véronique ULg; china, bernard; Daube, Georges ULg

Poster (2001)

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailInfections virales humaines d'origine alimentaire
Scipioni, Alexandra ULg; Daube, Georges ULg; Thiry, Etienne ULg

in Point Vétérinaire (2001), 32

Detailed reference viewed: 37 (1 ULg)
Full Text
Peer Reviewed
See detailBacterial Intestinal Flora Associated with Enterotoxaemia in Belgian Blue Calves
Manteca, Christophe; Daube, Georges ULg; Pirson, Vincianne et al

in Veterinary Microbiology (2001), 81(1), 1-12

The enterotoxaemia syndrome in Belgian Blue calves is characterised by a high case fatality rate, sudden death, lesions of haemorrhagic enteritis of the small intestine and, quite often an absence of ... [more ▼]

The enterotoxaemia syndrome in Belgian Blue calves is characterised by a high case fatality rate, sudden death, lesions of haemorrhagic enteritis of the small intestine and, quite often an absence of other clinical signs but its cause has not been yet identified. As a first step in this identification, the aerobic and anaerobic intestinal flora of a population of 78 calves, originating from farms located in southern Belgium and that died in circumstances defined as "calf enterotoxaemia" (study population) and of 64 calves that died in other circumstances (control population) were studied qualitatively and quantitatively. The colonies were identified after subcultures with appropriate API sugar sets. Anaerobically Clostridium perfringens was isolated in higher numbers (mean values of 10(7)-10(7.5) colony forming units (CFU) versus 10(4)-10(5) CFU per ml of intestinal content) and from more animals (79 versus 19%) in the study population than in the control population, although individual results from both populations could overlap. Other clostridial species, i.e. mainly urease-negative C. sordellii and C. bifermentans, were isolated in high numbers (>10(6) CFU per ml of intestinal content) from a few animals in the study population only. All but one of the 705 C. perfringens isolates from both populations belonged to the A toxin type and none of the urease-negative C. sordellii was toxigenic. Gram-negative anaerobes were not isolated in high numbers from any of the samples. Aerobically beta-haemolytic E. coli were significantly more frequent among the study population, but were isolated from only 25% of the animals. Salmonella Typhimurium was isolated from only two animals in the study population. Less than 1% of the E. coli isolated were verotoxigenic and one-third were necrotoxigenic. At this stage only non-enterotoxigenic type A C. perfringens are thus statistically associated with the enterotoxaemia syndrome in Belgian Blue calves and fulfil the first of the Koch's postulates. [less ▲]

Detailed reference viewed: 98 (2 ULg)
Peer Reviewed
See detailMicro-organismes pathogènes et viande : la traçabilité alliée de la sécurité.
Daube, Georges ULg

in Extrait du Bulletin de la Société Royale des Sciences de Liège (2001), 1

Detailed reference viewed: 22 (0 ULg)
Full Text
Peer Reviewed
See detailAntimicrobial susceptilities of Campylobacter strains isolated from food animals in Belgium.
Van Looveren, M.; Daube, Georges ULg; De Zutter, L. et al

in Journal of Antimicrobial Chemotherapy (2001), 48

Afin d’assurer une transparence tout le long de la chaîne de transformation de la viande, il faut disposer d’un système de traçabilité performant. La traçabilité administative montre des limites que ... [more ▼]

Afin d’assurer une transparence tout le long de la chaîne de transformation de la viande, il faut disposer d’un système de traçabilité performant. La traçabilité administative montre des limites que l’utilisation de marqueurs génétiques pourrait surmonter. Le génome de chaque individu possède des différences de séquences, à la base du polymorphisme génétique, dont les marqueurs génétiques sont les témoins. Parmi ceux-ci, deux classes semblent s’imposer en matière de traçabilité : les microsatellites et les polymorphismes simple nucléotide. Les microsatellites sont caractérisés par un degré de polymorphisme important avec de nombreux allèles pour un même locus. De plus, leur détection se fait directement par amplification itérative. Techniquement, le problème se pose lors de la détection simultanée de plusieurs microsatellites où le patron obtenu est difficile à interpréter. Les polymorphismes simple nucléotide sont répresentés par des mutations ponctuelles dans la séquence nucléotidique. Ils sont fréquents, stables, répartis de façon aléatoire et généralement bialléliques. La détection se fait soit par hybridation sur des biopuces, soit par spectrométrie de masse ou par d’autres techniques facilement automatisables. Cette automatisation est nécessaire pour pouvoir tester un grand nombre de polymorphismes simultanément sur un grand nombre d’échantillons, permettant ainsi de diminuer les coûts. L’avantage majeur des polymophismes simple nucléotide est le signal binaire obtenu, son désavantage est le caractère population- spécifique de ce type de marqueur. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Peer Reviewed
See detailAntimicrobial susceptilities of Salmonella strains isolated from food animals.
Van Looveren, M.; Daube, Georges ULg; De Zutter, L. et al

in Antimicrobial Agents and Chemotherapy (2001), 48

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailTraçabilité dans la filière viande. 2. Les marqueurs génétiques.
China, B.; Evrard, V.; Noirfalise, R. et al

in Annales de Médecine Vétérinaire (2001), 145

Afin d’assurer une transparence tout le long de la chaîne de transformation de la viande, il faut disposer d’un système de traçabilité performant. La traçabilité administative montre des limites que ... [more ▼]

Afin d’assurer une transparence tout le long de la chaîne de transformation de la viande, il faut disposer d’un système de traçabilité performant. La traçabilité administative montre des limites que l’utilisation de marqueurs génétiques pourrait surmonter. Le génome de chaque individu possède des différences de séquences, à la base du polymorphisme génétique, dont les marqueurs génétiques sont les témoins. Parmi ceux-ci, deux classes semblent s’imposer en matière de traçabilité : les microsatellites et les polymorphismes simple nucléotide. Les microsatellites sont caractérisés par un degré de polymorphisme important avec de nombreux allèles pour un même locus. De plus, leur détection se fait directement par amplification itérative. Techniquement, le problème se pose lors de la détection simultanée de plusieurs microsatellites où le patron obtenu est difficile à interpréter. Les polymorphismes simple nucléotide sont répresentés par des mutations ponctuelles dans la séquence nucléotidique. Ils sont fréquents, stables, répartis de façon aléatoire et généralement bialléliques. La détection se fait soit par hybridation sur des biopuces, soit par spectrométrie de masse ou par d’autres techniques facilement automatisables. Cette automatisation est nécessaire pour pouvoir tester un grand nombre de polymorphismes simultanément sur un grand nombre d’échantillons, permettant ainsi de diminuer les coûts. L’avantage majeur des polymophismes simple nucléotide est le signal binaire obtenu, son désavantage est le caractère population- spécifique de ce type de marqueur. [less ▲]

Detailed reference viewed: 81 (4 ULg)
Full Text
Peer Reviewed
See detailTraçabilité dans la filière viande. I. La traçabilité administrative.
Evrard, V.; China, B.; Noirfalise, R. et al

in Annales de Médecine Vétérinaire (2001), 145

Le secteur de la viande a été secoué ces dernières années par quelques scandales, tels ceux des hormones et de la dioxine, avec pour conséquences une perte de confiance de la part du consommateur et une ... [more ▼]

Le secteur de la viande a été secoué ces dernières années par quelques scandales, tels ceux des hormones et de la dioxine, avec pour conséquences une perte de confiance de la part du consommateur et une perturbation du marché de la viande. Pour redresser l’image des produits carnés belges, il est important de pouvoir en déterminer et en garantir l’origine. En Belgique, il existe divers systèmes de traçabilité administrative dont le principal est le système SANITEL qui comprend un système automatisé de traitement de données relatives à l’identification et l’enregistrement des animaux. Au-delà de l’aspect légal et réglementaire, différentes initiatives, visant une amélioration de la qualité, fleurissent : "les labels". Ceux-ci intègrent fréquemment la traçabilité dans leur cahier des charges. La traçabilité administrative n’est pas infaillible, la perte de documents et les fraudes peuvent ternir l’image de celle-ci. C’est pourquoi le système documentaire a été associé aux empreintes génétiques des animaux. 5 [less ▲]

Detailed reference viewed: 115 (3 ULg)