References of "Daube, Georges"
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See detailImpact de l’inuline sur l’activité des disaccharidases intestinales : une nouvelle cible contribuant à l’effet hypoglycémiant des prébiotiques
Neyrinck, Audrey M.; Theulier, Pauline; Jouret, Alexandra et al

Conference (2014, December)

Introduction et but de l’étude: Il est établi que l’administration de prébiotiques de type fructanes permet de diminuer la glycémie chez l’homme –un effet ayant abouti à une allégation par l’EFSA- et dans ... [more ▼]

Introduction et but de l’étude: Il est établi que l’administration de prébiotiques de type fructanes permet de diminuer la glycémie chez l’homme –un effet ayant abouti à une allégation par l’EFSA- et dans différents modèles murins d’obésité. Les mécanismes moléculaires sous-jacents restent à démontrer, et jusqu'à présent, sont évoqués une modulation de l'expression de gènes clés régulateurs du métabolisme énergétique, une modulation de la fonction endocrine de l'intestin et un changement de la sensibilité à l'insuline. Nous avons testé l’hypothèse selon laquelle l’inuline issue de la racine de chicorée change la digestibilité du saccharose, et avons mis en relation cet effet avec la modulation du microbiote intestinal. Matériel et méthodes: Un test de tolérance orale au saccharose (TTOS) a été réalisé sur des souris nourries avec un régime standard. Après 6h de jeune, les souris ont été gavées soit avec une solution de saccharose (3 g.kg-1) supplémentée avec de l’inuline (Fibruline® 5%), soit avec du saccharose seul. La glycémie et l’insulinémie ont été suivies durant 2h. Un TTOS a été appliqué également chez des souris préalablement nourries durant 20 jours avec l’inuline (5% dans un régime standard). L’activité des disaccharidases de la bordure en brosse ainsi que la composition du microbiote intestinal par pyroséquençage et par PCR quantitative de l’ADNr16S caecal ont été évaluées. L’activité de l’a-glucosidase a été évaluée in vitro en présence ou non d’inuline. Résultats et Analyse statistique: La glycémie et la réponse insulinémique sont significativement plus faibles 2h après le challenge avec la solution de saccharose lorsque l’inuline est ajoutée à la solution de gavage. L’analyse in vitro ne permet pas de mettre en évidence un effet inhibiteur direct de l’inuline sur l’a-glucosidase. Après 20 jours de supplémentation en inuline, l’aire sous la courbe (AUC) pour la glycémie lors de l’OTTS diminue significativement chez les souris ayant reçu l’inuline alors que les taux d’insuline sont inchangés. Par ailleurs, l’activité des disaccharidases (maltase, lactase, sucrase) est plus faible après la supplémentation chronique en inuline. En parallèle, une analyse de l’ARNr 16S révèle des changements bactériens importants au niveau du contenu caecal un mois après l’administration d’inuline, en ce compris une augmentation du nombre de Bifidobactéries. Conclusion: L’inuline est capable de diminuer la digestibilité de glucides indépendamment d’un changement de la composition du microbiote. L’arrivée de ces glucides au niveau du (caeco-)colon pourrait participer aux changement bactériens observés après une supplémentation chronique en inuline. En outre, l’administration d’inuline exerce un effet hypoglycémiant qui ne s’accompagne pas d’un changement des taux d’insuline, suggérant une amélioration de la sensibilité à l’insuline; cet effet pourrait s’expliquer en partie la baisse d’activité des disaccharidases. Ces résultats renforcent l’intérêt de l’ingestion de prébiotique de type inuline dans le contexte des désordres glucidiques associés ou non à l’obésité (diabète). [less ▲]

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See detailP180: Intérêt de la modulation du microbiote intestinal par les oligosaccharides non digestibles dans le contrôle de la leucémie et de la cachexie cancéreuse
Bindels, L.B.; Neyrinck, A.M.; Salazar, N. et al

in Nutrition Clinique et Metabolisme (2014, December), 28(S1), 162

Il est à présent clairement établi que l’ensemble des bactéries présentes dans l’intestin (le microbiote intestinal) est capable d’influencer l’homéostasie énergétique et immunitaire de son hôte. Nous ... [more ▼]

Il est à présent clairement établi que l’ensemble des bactéries présentes dans l’intestin (le microbiote intestinal) est capable d’influencer l’homéostasie énergétique et immunitaire de son hôte. Nous avons testé l’hypothèse selon laquelle une modulation du microbiote intestinal par des oligosaccharides issus de la pectine (POS) ou de l’inuline (INU) permet d’interférer avec la progression de la leucémie et des désordres métaboliques associés. [less ▲]

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See detailComparison of gut microbiota highfat induced modifications in young and old mice models
Taminiau, Bernard ULg; Neyrinck, A.; Delhalle, Laurent et al

Poster (2014, October 17)

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See detailHepatoprotective effects of crude rhubarbe extract extend to the modulation of gut microbiota in a murine acute alcohol-induced steato-hepatitis model
Taminiau, Bernard ULg; Neyrinck, A.M.; Nezer, Carine et al

Conference (2014, October 17)

Binge drinking is emerging and alarming social and health problem, leading to alcoholic liver disease (ALD). It is characterized by hepatic steatosis coupled with inflammation. A way to limit the ... [more ▼]

Binge drinking is emerging and alarming social and health problem, leading to alcoholic liver disease (ALD). It is characterized by hepatic steatosis coupled with inflammation. A way to limit the evolution of ALD would be the use of bioactive compounds to reduce fat accumulation and inflammation. A specific crude rhubarb extract has been tested in a murine model of acute alcohol-induced steato-hepatitis. 18 male C57BL/6J mice has been fed with a standard diet, enriched with (OH-EV1 group) or without the rhubarb extract (CT and OH groups) for 17 days. Groups OH and OH-EV1 were then submitted to binge drinking and euthanazed. Physiological parameters have been measured. Moreover, bacterial ceacal contents were analyzed by pyrosequencing and 16S rDNA v1-V3 targeted metagenomic analysis. Inflammatory markers levels in liver and total hepatic cholesterol level show that the standardized extract limits inflammation and lipid accumulation. Moreover, metagenomic analysis revealed the composition of major bacterial populations in caecum. Several populations were statistically influenced by the rhubarb enriched diet compared normal diet as shown after binge drinking. Among these populations, few were strongly correlated with the levels of inflammatory markers in the liver. We targeted 3 populations for further analysis : two Alistipes populations and Parabacteroides goldsteinii. The Alistipes belong to yet undescribed species. Based upon the only nucleic trace from pyrosequecing, 3 quantitative RT-PCR tests were created to target these populations in gut samples. Quantitative determination of these populations confirmed the statistical correlation with inflammatory markers and results from metagenomic study. [less ▲]

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See detailDiversity of Clostridium difficile isolates from humans and animals
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; Korsak Koulagenko, Nicolas ULg et al

Scientific conference (2014, October 17)

Clostridium difficile is an important cause of infectious diarrhea in hospitals. The major risk factors for the development of nosocomial C. difficile associated disease include antibiotic therapy and ... [more ▼]

Clostridium difficile is an important cause of infectious diarrhea in hospitals. The major risk factors for the development of nosocomial C. difficile associated disease include antibiotic therapy and increasing age. In animals, as pigs, calves and horses, C. difficile also seems to be an important cause of enteric disease. The main objective of this study was to characterize and compare animal and human C. difficile strains with respect to the PCR-ribotype and the antibiotic resistance. Multilocus sequence typing (MLST) and multiple-Locus Variable number tandem repeat analysis (MLVA) were performed in order to study clonal relationships of the isolates. Human C. difficile isolates were obtained from care home residents and hospitalized patients. Animal isolates were collected from stool samples and carcasses of pigs and cattle at slaughter. An identification of the strains was performed by PCR-ribotyping. Further characterization was performed by antibiotic resistance, MLST and MLVA analysis. A neighbourd-joining phylogenetic three was constructed in order to determine the correlation between human and food isolates. A great variety of PCR ribotypes was found among the animal isolates, including PCR ribotypes 078 and 014. The most prevalent PCR-ribotypes in the nursing home were PCR-ribotypes 027 and 020. A high resistance to moxifloxacin, erythromycin, gentamicin and clindamycin was detected for some of the strains. Phylogenetic analysis showed that human and animal isolates with the same PCR-ribotype cluster in the same lineage, suggesting a potential risk of interspecies transmission. [less ▲]

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See detailEvaluation of morphological and functional characteristics of Carnobacterium maltaromaticum isolated from vacuum-packaged beef with long shelf life
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Ndedi Ekolo, François ULg et al

Poster (2014, October 17)

Carnobacterium maltaromaticum is a lactic acid bacterium, and many lactic acid bacteria associated with meat are known for their bactericidal or bacteriostatic activity against other strains, species or ... [more ▼]

Carnobacterium maltaromaticum is a lactic acid bacterium, and many lactic acid bacteria associated with meat are known for their bactericidal or bacteriostatic activity against other strains, species or genera of bacteria. The presence of certain lactic acid bacteria adapted to a low temperature in fresh meat could extend the shelf life and improve the microbial stability and safety of this product. The aim of this study was to perform a morphological and functional characterization of a C. maltaromaticum strain with a potential bioprotective effect isolated from vacuum packaged beef with very long shelf life. The morphological, biochemical and enzymatic profiles, the influence of different temperatures and atmospheres, and the microbial stability of fresh beef inoculated with the C. maltaromaticum strain were evaluated. The isolated C. maltaromaticum strain presented similar morphological, biochemical and enzymatic profiles as those of two reference strains (LMG 11393 and LMG 22902). Among the studied conditions, a temperature of +12 °C and an atmosphere poor in oxygen were optimal for the growth of C. maltaromaticum. Vacuum packing is therefore suitable for this bacterium. An antimicrobial effect against Enterobacteriaceae was highlighted on inoculated fresh meat stored under N2. The functional characterization of this isolate will be further pursued by a genotypic characterization. Special attention will be taken to study its bioprotective properties. [less ▲]

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See detailNon digestible oligosaccharides modulate the gut microbiota to control the development of leukemia and associated cachexia
Bindels, L.B.; Neyrinck, A.; Salazar, N. et al

Conference (2014, October)

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See detailPhylogenomic comparison of 18 clinical Escherichia coli strains belonging to serogroups O5 and O118 isolated from bovine and human
Taminiau, Bernard ULg; Ogura, Y.; Hayashi, T. et al

Conference (2014, October)

In developed countries enterohaemorrhagic Escherichia coli (EHEC) are responsible for small- or large-scale outbreaks of uncomplicated diarrhoea, haemorrhagic colitis and/or haemolytic–uraemic syndrome ... [more ▼]

In developed countries enterohaemorrhagic Escherichia coli (EHEC) are responsible for small- or large-scale outbreaks of uncomplicated diarrhoea, haemorrhagic colitis and/or haemolytic–uraemic syndrome (HUS) in human and cattle. If the two major EHEC serotypes in Europe well studied and characherized, several subdominant serotypes such as O5 and O118 are present in cattle and can act as a reservoir for potential outbreaks. This study present the phylogenomic comparison of 10 O118 and 8 O5 clinical E. coli strains that were isolated from cattle and human diarrhea. Genomic DNA was isolated from an active growing colony and sequenced on a MySeq apparatus. The raw sequences from the 18 genomes were assembled into scaffolds using an in-house pipeline and subjected to a quick automated annotation pipeline. The metabolic models of the strains were elaborated and compared. We used the contigs data to determine the MLST sequence types and virulotypes. A scheme of 20 houskeeping and virulence determinents were concatenated and used to select the most ancestral strains for both serotypes. This ancestral strain was selected for another run of sequencing and used as the anchor for a phylogenomic analysis of the 18 strains. The phylogenomic tree allowed us to analyse if the source parameter (human or bovine) could be used to cluster strains. This study shows that genomic sequencing has become a quick and efficient tool that can be used to replace numerous existing typing analysis methods by an unique analysis scheme. [less ▲]

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See detailRelatedness of human, animal and food Clostridium difficile strains
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; Avesani, Véronique et al

Poster (2014, September 18)

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See detailMicrobial diversity and dynamics during Kombucha fermentation
Coton, M.; Pawtowski, A.; Taminiau, Bernard ULg et al

Poster (2014, September 01)

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