References of "Dandrifosse, Guy"
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See detailSpermine-induced modifications of intestinal urea cycle
Peulen, Olivier ULg; Gharbi, Myriam ULg; Powroznik, Brigitte et al

in Pflügers Archiv : European Journal of Physiology (2004)

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See detailShort-term effects of spermine ingestion on the small intestine: a comparison of suckling and weaned rats
Peulen, Olivier ULg; Deloyer, Patricia; Dandrifosse, Guy ULg

in Reproduction Nutrition Development (2004), 44(4, Jul-Aug), 353-364

We have previously shown that spermine, shortly after its ingestion, can induce the alteration of the morphology of the small intestine of suckling rats. It was proposed that this alteration is due to ... [more ▼]

We have previously shown that spermine, shortly after its ingestion, can induce the alteration of the morphology of the small intestine of suckling rats. It was proposed that this alteration is due to polyamine accumulation inside the epithelial cells. This could also be related to the fact that the intestine of the suckling rat is in an immature state. To shed light on this issue, disaccharidase and alkaline phosphatase activity assays, protein, DNA and RNA content measurements and polyamine concentration analysis were performed on the small intestine of suckling and weaned Wistar rats treated with spermine. Spermine did not induce the same intestinal alterations in weaned rats compared to suckling animals. Indeed, in sucklings, spermine administration induced a decrease of the protein, DNA, putrescine and spermidine intestinal content, suggesting a cell loss. The cell loss impaired the activity of intestinal enzymes: lactase, maltase and alkaline phosphatase. In weaned rats, the same treatment did not alter these parameters. Exogenous spermine by itself is not sufficient to induce the alterations described here and previously. The maturity degree of the small intestine could be the basis of this process. [less ▲]

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See detailEnhancement of lysozyme stability and activity by polyamines
Powroznik, Brigitte; Gharbi, Myriam ULg; Dandrifosse, Guy ULg et al

in Biochimie (2004), 86(9-10, Sep-Oct), 651-656

Spermine, a low molecular weight polyamine, administered orally to suckling rats induces the maturation of the small intestine. In this organ, lysozyme is an important component of the innate immunity. In ... [more ▼]

Spermine, a low molecular weight polyamine, administered orally to suckling rats induces the maturation of the small intestine. In this organ, lysozyme is an important component of the innate immunity. In this report, we analysed the binding of spermine to lysozyme and its effect on thermal inactivation of the protein by spectroscopy techniques. The activity of the enzyme was analysed in presence of spermine by lysoplate technique. We studied the effects of spermine ingestion by suckling rats on intestinal lysozyme activity and gene expression. We reported that spermine binds to lysozyme and increases in vitro the thermal stability and the activity of the protein. When administered orally to suckling rats, spermine increases the lysozyme activity in jejunum, but not in ileum. This increase is not due to a modification of the gene expression. The observed effects lead us to postulate that spermine could be used in some mammals as a promoter of the innate immunity. (C) 2004 Elsevier SAS. All rights reserved. [less ▲]

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See detailStudy of the interaction of polycations with human red blood cells
Grandfils, Christian ULg; Barakat, I; Verraes, S et al

Conference (2003, October 23)

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See detailStudy of the interaction of polycations with human red blood cells
Grandfils, Christian ULg; Barakat, I; Verraes, S et al

Conference (2003, May 25)

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See detailSpermine induces precocious development of the spleen in mice
Jolois, Olivier ULg; Peulen, Olivier ULg; Collin, Serge ULg et al

in Experimental Physiology (2002), 87(1), 69-75

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats, it induces maturation of the intestinal tract (liver ... [more ▼]

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats, it induces maturation of the intestinal tract (liver, pancreas and small intestine). Here we show that this polyamine is able to induce precocious intestinal and splenic development in suckling mice. In fact, in 15-day-old mice which had received spermine orally twice daily for 3 days we observed an increase in the ratio of white pulp surface to total spleen surface in comparison with untreated mice. The two macrophage subsets of the marginal zone and the B-cell population were more developed and reached the development level of 5- or 10-week-old mice. The proliferation rate of B-cells was increased by spermine administration to pups. These observations suggest that spermine might play a role in immune system development; further investigation of its effects are intended, namely the evaluation of its capacity to enhance defence during the neonatal period. [less ▲]

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See detailEffect of dietary polyamines and amino acids on the polyamine content of rat milk
Peulen, Olivier ULg; Dandrifosse, Guy ULg

in Morgan, D.; Hirvi, T.; Dandrifosse, Guy (Eds.) et al COST 917 Biologically active amines in food. Volume VI: Biologically active amines: metabolism and physiology and Biologically active amines in food processing and production of biologically active amines by bacteria (2002)

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See detailPolyamines as essential substances in metabolism
Dandrifosse, Anne-catherine; Deloyer, Patricia; Loret, Suzanne et al

in Trends in Comparative Biochemistry and Physiology (2002), 9

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See detailSpermine-Induced Alteration of Small Intestine in Suckling Rat: Involvement of Apoptosis or Zn2+ Enzymes?
Peulen, Olivier ULg; Denis, Ghislaine; Defresne, Marie-Paule ULg et al

in Digestive Diseases & Sciences (2001), 46(11), 2490-8

Polyamines are of great importance in several physiological processes, such as cell proliferation and differentiation. The ingestion of spermine by suckling rats induces precocious maturation of their ... [more ▼]

Polyamines are of great importance in several physiological processes, such as cell proliferation and differentiation. The ingestion of spermine by suckling rats induces precocious maturation of their small intestine. Shortly after ingestion, spermine produces cell elimination at the villous top. The origin of this exfoliation was investigated to determine whether it was due to apoptosis. Wistar rats were orally treated with spermine. Apoptosis was analyzed in their small intestine by Tdt-mediated dUTP-fluorescein nick-end labeling reaction, caspase-3-like analysis, and DNA laddering. Polyamine content was measured by HPLC. The intestinal transitory alteration appeared as soon as 2 hr after spermine administration. Apoptosis events increased strongly at the same moment in the small intestine. They were evidenced by Tdt-mediated dUTP-fluorescein nick-end labeling analysis, DNA laddering, and caspase-3-like activity. Changes observed are consistent with apoptosis, but caspase inhibitor did not reduce intestinal alteration, as did Zn2+ chelator. [less ▲]

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See detailUse of fluorescent polyethylene glycols (PEGs) to assess intestinal permeability in vitro
Loret, S; Pirson, L; Nollevaux, G et al

in Pflügers Archiv : European Journal of Physiology (2001, September)

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See detailDietary Polyamines and Non-Neoplastic Growth and Disease
Deloyer, Patricia; Peulen, Olivier ULg; Dandrifosse, Guy ULg

in European Journal of Gastroenterology & Hepatology (2001), 13(9), 1027-32

This review presents the data that are now available concerning the effects of dietary polyamines at either postnatal or adult stages in non-neoplastic growth and disease. Polyamines provided by food have ... [more ▼]

This review presents the data that are now available concerning the effects of dietary polyamines at either postnatal or adult stages in non-neoplastic growth and disease. Polyamines provided by food have a potential role in growth and development of the digestive system in neonatal mammals (and fishes). In humans, this property could be of importance in preventing the appearance of food allergies. Dietary polyamines also seem necessary for the maintenance of normal growth and general properties of adult digestive tract. Their possible therapeutic effects have been investigated in gastric, intestinal, and, more recently, whole-body healing. [less ▲]

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See detailUse of fluorescent polyethylene glycols (PEGs) to assess intestinal permeability in vitro
Loret, S; Pirson, L; Nollevaux, G et al

in Pflügers Archiv : European Journal of Physiology (2001), 442(5), 187

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See detailSpermine induces precocious development of the spleen in Wistar rat
Peulen, Olivier ULg; Jolois, Olivier ULg; Galopin, Catherine et al

in Trends in Comparative Biochemistry and Physiology (2001), 8

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See detailUrinary excretion of high Mr PEG (4000 Da) in rats
Nollevaux, G; Loret, S; Barakat, I et al

Conference (2000, November 18)

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See detailUrinary excretion of high Mr PEG (>4000 Da) in rats
Nollevaux, G; Loret, S; Barakat, I et al

Poster (2000, November 18)

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See detailMaturation of the small intestine is induced by spermine but not by other similar amines
Peulen, Olivier ULg; Grandfils, Christian ULg; Dandrifosse, Guy ULg

in Pflügers Archiv : European Journal of Physiology (2000, September 19), 440

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See detailBiocompatibility of circulating polyethylene glycols (PEG): an approach using bovine serum albumin (BSA) as a protein model
Loret, S; Gilson, Ph; Barakat, I et al

Poster (2000, September 19)

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