Involvement of Epithelial-to-Mesenchymal Transitions on the interactions between Circulating Tumor Cells and the coagulation system: implication for the metastatic spread.
Bourcy, Morgane ; Suarez-Carmona, Meggy ; Francart, Marie-Emilie et al
Poster (2015, February)Detailed reference viewed: 15 (3 ULg)
Myoferlin: an indispensable component in VEGFA secretion by pancreas cancer cells
Fahmy, Karim ; Peulen, Olivier ; Castronovo, Vincenzo et al
Poster (2015, January 31)
In this poster, our laboratory showed the importance of myoferlin, a biomarker of pancreas cancer, in the controle of VEGF-A mediated angiogenesis. Our laboratory showed that silencing myoferlin in ... [more ▼]
In this poster, our laboratory showed the importance of myoferlin, a biomarker of pancreas cancer, in the controle of VEGF-A mediated angiogenesis. Our laboratory showed that silencing myoferlin in pancreas cancer cells, BxPC-3, provoques a decrease in cell prolifération in vitro and a decrease in tumor volumes in animal model. Myoferlin silencing also provokes a decrease in VEGF-A secretion in the conditioned medium and that decrease was abserved in the animal model as a decrease in microvessels dencity. It appeared that this decrease in secretion is due to a a blockage in the exocytosis. Our data also showed a significate correlation between myoferlin expression and microvessels density in patients section. [less ▲]Detailed reference viewed: 50 (10 ULg)
Clinical significance of MT4-MMP and EGFR expression in Breast Cancer
Yip, Cassandre ; PAYE, Alexandra ; Truong, Alice et al
Scientific conference (2015, January 31)Detailed reference viewed: 19 (7 ULg)
Grille de correction: déclinaisons de la perception et des réalisations
Bonnet, Pierre ; Delvenne, Philippe ; Defaweux, Valérie et al
Conference (2015, January 29)
Un outil disponible on-line pour la correction des questions ouvertes à réponse courte ou longue (QROC et QROL) est utilisé depuis plusieurs années au sein de l’ULg. Les copies réponse des étudiants sont ... [more ▼]
Un outil disponible on-line pour la correction des questions ouvertes à réponse courte ou longue (QROC et QROL) est utilisé depuis plusieurs années au sein de l’ULg. Les copies réponse des étudiants sont scannées et corrigées sur un écran d’ordinateur. L’enseignant fournit l’énoncé de la question et une grille de correction suivant un canevas prédéfini. La correction évalue une série de critères regroupés par 4 à l’écran. Chaque critère défini est évalué au moyen d’une échelle horizontale allant de 0 à 100% suivant que le critère n’est pas rencontré (0%) ou qu’il est totalement développé (100%) par l’étudiant. L’enseignant peut graduer cette échelle en y indiquant des repères (indices). La pondération des différents critères est définie à priori ou à posteriori. Sur cette base de travail commune, nous analysons la façon dont cette grille est utilisée pour corriger des questions relatives à des matières médicales différentes enseignées par différents professeurs : histologie, anatomie, physiologie et anatomopathologie. L’analyse fait ressortir que malgré un canevas commun, les modalités de correction sont variables ce qui témoigne de la différence existant entre les matières, les compétences évaluées et, probablement, la sensibilité des enseignants. A l’inverse, ceci démontre la souplesse de l’outil adaptable à des types d’évaluations différents. [less ▲]Detailed reference viewed: 62 (21 ULg)
EXPEL: A Novel Non-Destructive Method for Mining Soluble Tumor Biomarkers
Costanza, Brunella ; ; Bellahcene, Akeila et al
Poster (2015, January)Detailed reference viewed: 23 (6 ULg)
Cervical (pre)neoplastic microenvironment promotes the emergence of tolerogenic dendritic cells via RANKL secretion
Demoulin, Stéphanie ; SOMJA, Joan ; et al
in OncoImmunology (2015), 6Detailed reference viewed: 52 (22 ULg)
Tumeurs rhabdoïdes néonatales : à propos de deux cas.
; FORGET, Patricia ; Piette, Caroline et al
in Tijdschrift van de Belgische Kinderarts = Journal du Pédiatre Belge (2015), 17(1),Detailed reference viewed: 20 (2 ULg)
Soluble factors regulated by epithelial-mesenchymal transition mediate tumour angiogenesis and myeloid cell recruitment.
Suarez-Carmona, Meggy ; Bourcy, Morgane ; et al
in Journal of Pathology (The) (2015), 236(4), 491-504
Epithelial-to-mesenchymal transition (EMT) programs provide cancer cells with invasive and survival capacities that might favor metastatic dissemination. Whilst signaling cascades triggering EMT have been ... [more ▼]
Epithelial-to-mesenchymal transition (EMT) programs provide cancer cells with invasive and survival capacities that might favor metastatic dissemination. Whilst signaling cascades triggering EMT have been extensively studied, the impact of EMT on the crosstalk between tumor cells and the tumor microenvironment remains elusive. We aimed to identify EMT-regulated soluble factors that facilitate the recruitment of host cells in the tumor. Our findings indicate that EMT phenotypes relate to the induction of a panel of secreted mediators, namely IL-8, IL-6, sICAM-1, PAI-1 and GM-CSF, and implicate the EMT-transcription factor Snail as a regulator of this process. We further show that EMT-derived soluble factors are pro-angiogenic in vivo (in the mouse ear sponge assay), ex vivo (in the rat aortic ring assay) and in vitro (in a chemotaxis assay). Additionally, conditioned medium from EMT-positive cells stimulates the recruitment of myeloid cells. In a bank of 40 triple-negative breast cancers, tumors presenting features of EMT were significantly more angiogenic and infiltrated by a higher quantity of myeloid cells compared to tumors with little or no EMT. Taken together, our results show that EMT programs trigger the expression of soluble mediators in cancer cells that stimulate angiogenesis and recruit myeloid cells in vivo, which might in turn favor cancer spread. [less ▲]Detailed reference viewed: 105 (26 ULg)
Development of anti-E6 pegylated lipoplexes for mucosal application in the context of cervical preneoplastic lesions
Lechanteur, Anna ; Furst, Tania ; Evrard, Brigitte et al
in International Journal of Pharmaceutics (2015)Detailed reference viewed: 107 (37 ULg)
Asporin Is a Fibroblast-Derived TGF-beta1 Inhibitor and a Tumor Suppressor Associated with Good Prognosis in Breast Cancer.
; ; et al
in PLoS medicine (2015), 12(9), 1001871
BACKGROUND: Breast cancer is a leading malignancy affecting the female population worldwide. Most morbidity is caused by metastases that remain incurable to date. TGF-beta1 has been identified as a key ... [more ▼]
BACKGROUND: Breast cancer is a leading malignancy affecting the female population worldwide. Most morbidity is caused by metastases that remain incurable to date. TGF-beta1 has been identified as a key driving force behind metastatic breast cancer, with promising therapeutic implications. METHODS AND FINDINGS: Employing immunohistochemistry (IHC) analysis, we report, to our knowledge for the first time, that asporin is overexpressed in the stroma of most human breast cancers and is not expressed in normal breast tissue. In vitro, asporin is secreted by breast fibroblasts upon exposure to conditioned medium from some but not all human breast cancer cells. While hormone receptor (HR) positive cells cause strong asporin expression, triple-negative breast cancer (TNBC) cells suppress it. Further, our findings show that soluble IL-1beta, secreted by TNBC cells, is responsible for inhibiting asporin in normal and cancer-associated fibroblasts. Using recombinant protein, as well as a synthetic peptide fragment, we demonstrate the ability of asporin to inhibit TGF-beta1-mediated SMAD2 phosphorylation, epithelial to mesenchymal transition, and stemness in breast cancer cells. In two in vivo murine models of TNBC, we observed that tumors expressing asporin exhibit significantly reduced growth (2-fold; p = 0.01) and metastatic properties (3-fold; p = 0.045). A retrospective IHC study performed on human breast carcinoma (n = 180) demonstrates that asporin expression is lowest in TNBC and HER2+ tumors, while HR+ tumors have significantly higher asporin expression (4-fold; p = 0.001). Assessment of asporin expression and patient outcome (n = 60; 10-y follow-up) shows that low protein levels in the primary breast lesion significantly delineate patients with bad outcome regardless of the tumor HR status (area under the curve = 0.87; 95% CI 0.78-0.96; p = 0.0001). Survival analysis, based on gene expression (n = 375; 25-y follow-up), confirmed that low asporin levels are associated with a reduced likelihood of survival (hazard ratio = 0.58; 95% CI 0.37-0.91; p = 0.017). Although these data highlight the potential of asporin to serve as a prognostic marker, confirmation of the clinical value would require a prospective study on a much larger patient cohort. CONCLUSIONS: Our data show that asporin is a stroma-derived inhibitor of TGF-beta1 and a tumor suppressor in breast cancer. High asporin expression is significantly associated with less aggressive tumors, stratifying patients according to the clinical outcome. Future pre-clinical studies should consider options for increasing asporin expression in TNBC as a promising strategy for targeted therapy. [less ▲]Detailed reference viewed: 74 (11 ULg)
Intérêts et limites de l'examen extemporané en pathologie thyroïdienne : Revue systématique de la littérature et évaluation fondée sur les épreuves
; ; THIRY, Albert et al
in Revue Médicale de Liège (2015), 70(12), 638-643Detailed reference viewed: 32 (3 ULg)
Belgian registery for gestationnal trophoblastic diseases: impact of central pathology review. A study of the BGOG in 237 patients.
TIMMERMANS, Marie ; DELCOMINETTE, Sarah ; et al
Poster (2015)Detailed reference viewed: 23 (1 ULg)
Belgian registery for gestational trophoblastic diseases: how does hysterectomy impact the management of GTD/GTN. A retrospective analyses of 11 cases.
DELCOMINETTE, Sarah ; ; DELBECQUE, Katty et al
Poster (2015)Detailed reference viewed: 9 (1 ULg)
18F-FDG PET/CT in the Management of Aortitis.
; Courtois, Audrey ; Nusgens-Richelle, Betty et al
in Clinical nuclear medicine (2015)
BACKGROUND: Aortitis is a generic term defined as an inflammatory condition involving the aortic wall, of infectious or noninfectious origin. This inflammatory process may deteriorate the aortic wall ... [more ▼]
BACKGROUND: Aortitis is a generic term defined as an inflammatory condition involving the aortic wall, of infectious or noninfectious origin. This inflammatory process may deteriorate the aortic wall, resulting in potentially life-threatening vascular complications. Therefore, it is important to establish a diagnosis as early as possible. PATIENTS AND METHODS: During a 4-year period, 428 consecutive patients referred to our department for aortic diseases underwent FDG PET/CT examinations. Among these, 18 patients (4.2%) were suspected to have aortitis. All of them had an initial positive FDG PET/CT uptake occurring in the aorta and major branches as evaluated by visual analysis of images and assessed with the final diagnosis of aortitis. During follow-up, after surgery and/or upon immunosuppressive treatment, each of these patients underwent a second PET/CT that was compared with the initial evaluation. In all cases, normalization of FDG uptake was correlated with clinical improvement. CONCLUSIONS: Our study aimed to illustrate the potential clinical value of functional monitoring with PET/CT in the management of aortitis. FDG PET/CT constitutes a valuable imaging modality to establish an early diagnosis, monitor disease progression and treatment, and evaluate vascular complication and relapse. We highlight the importance of an early detection of inflammatory large-vessel pathology, which may represent a major threat. [less ▲]Detailed reference viewed: 113 (21 ULg)
Hedgehog- and mTOR-targeted therapies for advanced basal cell carcinomas.
FRANCHIMONT, Claudine ; Hermanns-Lê, Trinh ; PAQUET, Philippe et al
in Future Oncology (2015), 11
Basal cell carcinomas (BCCs) are the most frequent human cancer. Over 90% of all BCCs have a mutation in patched homologue 1 (PTCH 1) or smoothened (SMO), two conducting proteins of the Hedgehog (Hh ... [more ▼]
Basal cell carcinomas (BCCs) are the most frequent human cancer. Over 90% of all BCCs have a mutation in patched homologue 1 (PTCH 1) or smoothened (SMO), two conducting proteins of the Hedgehog (Hh) pathway. They rarely progress deeply and metastasize; however, if they do, these advanced BCC become amenable to treatment by inhibiting the Hedgehog and the P13K–mTOR pathways. Such innovative drugs include vismodegib, cyclopamine, itraconazole, everolimus and a few other agents that are in early clinical development. [less ▲]Detailed reference viewed: 122 (26 ULg)
HMGB1 secretion during cervical carcinogenesis promotes the acquisition of a tolerogenic functionality by plasmacytoid dendritic cells
Demoulin, Stéphanie ; Herfs, Michael ; SOMJA, Joan et al
in International Journal of Cancer = Journal International du Cancer (2015), 137Detailed reference viewed: 56 (22 ULg)
Defensins: « simple » antimicrobial peptides or broad-spectrum molecules ?
Suarez-Carmona, Meggy ; Hubert, Pascale ; Delvenne, Philippe et al
in Cytokine & Growth Factor Reviews (2015), 26Detailed reference viewed: 32 (8 ULg)
Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction.
Herfs, Michael ; SOMJA, Joan ; et al
in International journal of cancer. Journal international du cancer (2015), 136
Recent studies have identified a putative cell of origin for cervical intraepithelial neoplasia (CIN) and cervical cancer at the squamo-columnar junction (SCJ) and suggest that these cells may not ... [more ▼]
Recent studies have identified a putative cell of origin for cervical intraepithelial neoplasia (CIN) and cervical cancer at the squamo-columnar junction (SCJ) and suggest that these cells may not regenerate following excision (LEEP). This study addressed the impact of SCJ excision on the temporal dynamics, histologic and viral (HPV) characteristics of recurrent CIN. One hundred thirty one consecutive patients treated by excision and attending follow-up visits were enrolled. We compared recurrent and initial CIN with attention to excision margins, timing of recurrence, CIN grade, HPV types, p16 immunophenotype, and SCJ immunophenotype. During the follow-up period (up to four years), sixteen (12.2%) recurrences were identified. Four (25%) were identified at the first follow-up visit, closely resembled the initial CIN 2/3 in grade and HPV type, and were typically SCJ marker positive [SCJ(+)], suggesting non-excised (residual) disease. Twelve (75%) manifested after the first postoperative visit and all were in the ectocervix or in mature metaplastic epithelium. All of the 12 delayed recurrences were classified as CIN 1 and were SCJ (-). Nine of 11 SCJ (-) recurrences (82%) followed regressed spontaneously. Taken together, these results show that new lesions developing from any HPV infection are delayed and occur within the ectocervix or metaplastic epithelium. This dramatically lower risk of CIN 2/3 following successful SCJ excision suggests that removal of the SCJ could be a critical variable in reducing the risk of subsequent CIN 2/3 and cervical cancer. (c) 2014 Wiley Periodicals, Inc. [less ▲]Detailed reference viewed: 61 (7 ULg)
Simulants of malignant melanoma
Pierard, Gérald ; Pierard-Franchimont, Claudine ; Delvenne, Philippe
in Oncology Reviews (2015), 9
During the recent period, dermoscopy has yielded improvement in the early disclosure of various atypical melanocytic neoplasms (AMN) of the skin. Beyond this clinical procedure, AMN histopathology remains ... [more ▼]
During the recent period, dermoscopy has yielded improvement in the early disclosure of various atypical melanocytic neoplasms (AMN) of the skin. Beyond this clinical procedure, AMN histopathology remains mandatory for establishing their precise diagnosis. Of note, panels of experts in AMN merely report moderate agreement in various puzzling cases. Divergences in opinion and misdiagnosis are likely increased when histopathological criteria are not fine-tuned and when facing a diversity of AMN types. Furthermore, some AMN have been differently named in the literature including atypical Spitz tumor, metastasizing Spitz tumor, borderline and intermediate melanocytic tumor, malignant Spitz nevus, pigmented epithelioid melanocytoma or animal-type melanoma. Some acronyms have been further suggested such as MELTUMP (after melanocytic tumor of uncertain malignant potential) and STUMP (after Spitzoid melanocytic tumor of uncertain malignant potential). In this review, such AMN at the exclusion of cutaneous malignant melanoma (MM) variants, are grouped under the tentative broad heading skin melanocytoma. Such set of AMN frequently follows an indolent course, although they exhibit atypical and sometimes worrisome patterns or cytological atypia. Rare cases of skin melanocytomas progress to loco regional clusters of lesions (agminate melanocytomas), and even to regional lymph nodes. At times, the distinction between a skin melanocytoma and MM remains puzzling. However, multipronged immunohistochemistry and emerging molecular biology help profiling any malignancy risk if present. [less ▲]Detailed reference viewed: 16 (3 ULg)
The importance of the tumor microenvironment in the therapeutic management of cancer
Pottier, Charles ; ; RONCARATI, Patrick et al
in Expert Review of Anticancer Therapy (2015), 15Detailed reference viewed: 64 (25 ULg)