NF-kappaB-induced KIAA1199 promotes survival through EGFR signalling.
Shostak, Kateryna ; ; Hubert, Pascale et al
in Nature communications (2014), 5
Constitutive activation of EGFR- and NF-kappaB-dependent pathways is a hallmark of cancer, yet signalling proteins that connect both oncogenic cascades are poorly characterized. Here we define KIAA1199 as ... [more ▼]
Constitutive activation of EGFR- and NF-kappaB-dependent pathways is a hallmark of cancer, yet signalling proteins that connect both oncogenic cascades are poorly characterized. Here we define KIAA1199 as a BCL-3- and p65-dependent gene in transformed keratinocytes. KIAA1199 expression is enhanced on human papillomavirus (HPV) infection and is aberrantly expressed in clinical cases of cervical (pre)neoplastic lesions. Mechanistically, KIAA1199 binds Plexin A2 and protects from Semaphorin 3A-mediated cell death by promoting EGFR stability and signalling. Moreover, KIAA1199 is an EGFR-binding protein and KIAA1199 deficiency impairs EGF-dependent Src, MEK1 and ERK1/2 phosphorylations. Therefore, EGFR stability and signalling to downstream kinases requires KIAA1199. As such, KIAA1199 promotes EGF-mediated epithelial-mesenchymal transition (EMT). Taken together, our data define KIAA1199 as an oncogenic protein induced by HPV infection and constitutive NF-kappaB activity that transmits pro-survival and invasive signals through EGFR signalling. [less ▲]Detailed reference viewed: 34 (4 ULg)
Downscalling tissue proteomics: analyses of limited amounts of FFPE tissue samples. Application to cervix cancer biomarker markers.
Longuespée, Rémi ; ; Smargiasso, Nicolas et al
Poster (2014)Detailed reference viewed: 25 (7 ULg)
Comparison of two FFPE preparation methods using label-free shotgun proteomics: Application to tissues of diverticulitis patients.
; Bertrand, Virginie ; Longuespée, Rémi et al
in Journal of proteomics (2014), 112C
Formalin-fixed paraffin-embedded (FFPE) specimens of patients are useful sources of materials for clinical research and have recently gained interest for use in the discovery of clinical proteomic ... [more ▼]
Formalin-fixed paraffin-embedded (FFPE) specimens of patients are useful sources of materials for clinical research and have recently gained interest for use in the discovery of clinical proteomic biomarkers. However, the critical step in this field is the ability to obtain an efficient and repeatable extraction using the limited quantities of material available for research in hospital biobanks. This work describes the evaluation of the peptide/protein extraction using FFPE sections treated by the following two methods before shotgun proteomic analysis: a commercial solution (FFPE-FASP) (filter aided sample preparation) and an antigen retrieval-derived protocol (On Slice AR). Their efficiencies and repeatabilities are compared using data-independent differential quantitative label-free analysis. FFPE-FASP was shown to be globally better both qualitatively and quantitatively than On Slice AR. FFPE-FASP was tested on several samples, and differential analysis was used to compare the tissues of diverticulitis patients (healthy and inflammatory tissues). In this differential proteomic analysis using retrospective clinical FFPE material, FFPE-FASP was reproducible and provided a high number of confident protein identifications, highlighting potential protein biomarkers. BIOLOGICAL SIGNIFICANCE: In clinical proteomics, FFPE is an important resource for retrospective analysis and for the discovery of biomarkers. The challenge for FFPE shotgun proteomic analysis is preparation by an efficient and reproducible protocol, which includes protein extraction and digestion. In this study, we analyzed two different methods and evaluated their repeatabilities and efficiencies. We illustrated the reproducibility of the most efficient method, FFPE-FASP, by a pilot study on diverticulitis tissue and on FFPE samples amount accessible in hospital biobanks. These data showed that FFPE is suitable for use in clinical proteomics, especially when the FFPE-FASP method is combined with label-free shotgun proteomics as described in the workflow presented in this work. [less ▲]Detailed reference viewed: 62 (16 ULg)
Cyanoacrylate skin surface strippings and the 3S-Bioket advent in tropical dermatology. A look from Liège.
PIERARD, Gérald ; Franchimont, Claudine ; PAQUET, Philippe et al
in Scientific World Journal (The) (2014), 2014
In the dermatopathology field, some simple available laboratory tests require minimum equipment for establishing a diagnosis. Among them, the cyanoacrylate skin surface stripping (CSSS), formerly named ... [more ▼]
In the dermatopathology field, some simple available laboratory tests require minimum equipment for establishing a diagnosis. Among them, the cyanoacrylate skin surface stripping (CSSS), formerly named skin surface biopsy or follicular biopsy, represents a convenient low cost procedure. It is a minimally invasive method collecting a continuous sheet of stratum corneum and horny follicular casts. In the vast majority of cases, it is painless and is unassociated with adverse events. CSSS can be performed in subjects of any age. The method has a number of applications in diagnostic dermatopathology and cosmetology, as well as in experimental dermatology settings. A series of derived analytic procedures include xerosis grading, comedometry, corneofungimetry, corneodynamics of stratum corneum renewal, corneomelametry, corneosurfametry, and corneoxenometry. [less ▲]Detailed reference viewed: 13 (5 ULg)
Tissue Proteomics for the Next Decade? Towards a Molecular Dimension in Histology
Longuespée, Rémi ; ; et al
in OMICS : A Journal of Integrative Biology (2014)Detailed reference viewed: 21 (3 ULg)
La dermatoporose, un "vintage" de l'atrophodermie et de la "peau transparente".
FRANCHIMONT, Claudine ; ; Hermanns-Lê, Trinh et al
in Revue Médicale de Liège (2014), 69
Le vieillissement de la peau se marque à terme par une atrophodermie, parfois sévère. La corticothérapie, qu’elle soit systémique ou topique, exerce un effet atrophiant qui copie l’effet du grand âge. Cet ... [more ▼]
Le vieillissement de la peau se marque à terme par une atrophodermie, parfois sévère. La corticothérapie, qu’elle soit systémique ou topique, exerce un effet atrophiant qui copie l’effet du grand âge. Cet état d’atrophodermie a été décrit comme la «peau transparente» il y a une quarantaine d’années, et il a reçu plus récemment la nouvelle dénomination de dermatoporose. Il est connu de longue date que cet état entraîne des conséquences cliniques parfois majeures qui conduisent à des lésions diverses sous forme de purpura de Bateman, de cicatrices stellaires, et de plaies faisant suite à des traumatismes mineurs. [less ▲]Detailed reference viewed: 26 (0 ULg)
Gynecologic and obstetric impact of the Ehlers-Danlos syndrome: clues from scrutinizing dermal ultrastructural alterations.
Hermanns-Lê, Trinh ; PIERARD, Gérald ; FRANCHIMONT, Claudine et al
in Gynecology (2014), 2(1),
Background: The Ehlers-Danlos syndrome (EDS) represents a heterogenous group of heritable connective tissue disorders chiefly characterized by joint laxity, increased skin distensibility and connective ... [more ▼]
Background: The Ehlers-Danlos syndrome (EDS) represents a heterogenous group of heritable connective tissue disorders chiefly characterized by joint laxity, increased skin distensibility and connective tissue fragility. Each EDS type corresponds to a multisystemic disorder with widespread implications. Physicians evoke the EDS diagnosis in presence of some typical cutaneous signs, such as atrophic scars and frequent hematomas. Methods: To revisit the transmission electron microscopy support from skin biopsies when screening EDS in gynecology-obstetrics. Results: EDS women commonly suffer from a series of gynecologic and obstetric disorders. Dermal ultrastructural changes are keynote features suggesting some EDS types. Conclusion: Gene mutations are mostly identified in the classic and vascular EDS types, but only in sporadic cases of the hypermobile EDS type which apparently represents the most frequent type. Dermal ultrastructural changes represent relevant diagnostic clues for the practitioner. Preconception counseling about possible complications is welcome. Management requires precise diagnosis of the EDS type. [less ▲]Detailed reference viewed: 33 (4 ULg)
Effect of N-acetylcysteine combined with infliximab on toxic epidermal necrolysis. A proof-of-concept study.
PAQUET, Philippe ; ; ROUSSEAU, Anne-Françoise et al
in Burns : Journal of the International Society for Burn Injuries (2014)Detailed reference viewed: 23 (4 ULg)
Le point de rosée en berne et la couche cornée hivernale.
PIERARD, Gérald ; FRANCHIMONT, Claudine ; Delvenne, Philippe
in Revue Médicale de Liège (2014), 69(2), 68-71
The frontier between the human body and the environment is largely represented by the stratum corneum. It is obvious that the skin aspect on some body sites appears dry and rough in some predisposed ... [more ▼]
The frontier between the human body and the environment is largely represented by the stratum corneum. It is obvious that the skin aspect on some body sites appears dry and rough in some predisposed individuals during winter season. The reductions in the outdoor temperature, ambient moisture and dew point are the main physical factors involved in this process. We present the aspects seen under ultraviolet light and scanning electron microscopy. Keywords : Stratum corneum - Dew point [less ▲]Detailed reference viewed: 13 (3 ULg)
Identification of a microRNA landscape targeting the PI3K/Akt signaling pathway in inflammation-induced colorectal carcinogenesis
JOSSE, Claire ; Bouznad, Nassim ; Geurts, Pierre et al
in American Journal of Physiology - Gastrointestinal and Liver Physiology (2014), 306
Inflammation can contribute to tumor formation; however, markers that predict progression are still lacking. In the present study, the well-established azoxymethane (AOM)/dextran sulfate sodium (DSS ... [more ▼]
Inflammation can contribute to tumor formation; however, markers that predict progression are still lacking. In the present study, the well-established azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced mouse model of colitis-associated cancer was used to analyze microRNA (miRNA) modulation accompanying inflammation-induced tumor development and to determine whether inflammation-triggered miRNA alterations affect the expression of genes or pathways involved in cancer. A miRNA microarray experiment was performed to establish miRNA expression profiles in mouse colon at early and late time points during inflammation and/or tumor growth. Chronic inflammation and carcinogenesis were associated with distinct changes in miRNA expression. Nevertheless, prediction algorithms of miRNA-mRNA interactions and computational analyses based on ranked miRNA lists consistently identified putative target genes that play essential roles in tumor growth or that belong to key carcinogenesis-related signaling pathways. We identified PI3K/Akt and the insulin growth factor-1 (IGF-1) as major pathways being affected in the AOM/DSS model. DSS-induced chronic inflammation downregulates miR-133a and miR-143/145, which is reportedly associated with human colorectal cancer and PI3K/Akt activation. Accordingly, conditioned medium from inflammatory cells decreases the expression of these miRNA in colorectal adenocarcinoma Caco-2 cells. Overexpression of miR-223, one of the main miRNA showing strong upregulation during AOM/DSS tumor growth, inhibited Akt phosphorylation and IGF-1R expression in these cells. Cell sorting from mouse colons delineated distinct miRNA expression patterns in epithelial and myeloid cells during the periods preceding and spanning tumor growth. Hence, cell-type-specific miRNA dysregulation and subsequent PI3K/Akt activation may be involved in the transition from intestinal inflammation to cancer. [less ▲]Detailed reference viewed: 75 (12 ULg)
Cervical cancer and HPV: Awareness and vaccine acceptability among parents in Morocco.
; ; et al
in Vaccine (2014), 32(3), 409-16
Cervical cancer is a major public health concern in Morocco where it represents the second most common and lethal cancer in women. Human papillomavirus (HPV) vaccines have been licensed in Morocco since ... [more ▼]
Cervical cancer is a major public health concern in Morocco where it represents the second most common and lethal cancer in women. Human papillomavirus (HPV) vaccines have been licensed in Morocco since 2008 but there are no available data on their acceptability. This study aimed to assess awareness of HPV and the vaccine, and to identify factors associated with acceptability of the vaccine among parents in Morocco. We carried out a questionnaire-based survey using face-to-face interviews in a sample of 852 parents (670 mothers and 182 fathers) with at least one unmarried daughter </=26 years. We collected data within public and private health centres and clinics in four regions in Morocco between July and August 2012. The main outcome measure was parental acceptability of the HPV vaccine for their daughter(s). Responses revealed very low awareness of HPV infection (4.7%) and the HPV vaccine (14.3%). None of the participants had vaccinated their daughter(s) against HPV and vaccine acceptability was low among mothers (32%) and fathers (45%). Higher education and income, previous awareness of the HPV vaccine and endorsement of the belief that a recommendation from the Ministry of Health or a doctor to have the vaccine would be encouraging, were associated with mothers' HPV vaccine acceptability. Non-acceptability among mothers was associated with having more than two daughters, believing the vaccine was expensive, lack of information and believing that whatever happens to an individual's health is God's will. The only factor associated with the fathers' acceptability of the vaccine was the cost of the vaccine. Increasing HPV and HPV vaccine awareness through educational campaigns, along with active recommendation by physicians and a publically funded vaccination programme could increase parental acceptability of the HPV vaccine in Morocco. [less ▲]Detailed reference viewed: 52 (2 ULg)
A Clinical and Pathological Overview of Vulvar Condyloma Acuminatum, Intraepithelial Neoplasia and Squamous Cell Carcinoma
LEONARD, Boris ; Kridelka, Frédéric ; DELBECQUE, Katty et al
in BioMed Research International (2014)Detailed reference viewed: 49 (19 ULg)
Hedgehog- and mTOR-targeted therapies for advanced basal cell carcinomas.
FRANCHIMONT, Claudine ; Hermanns-Lê, Trinh ; PAQUET, Philippe et al
in Current approaches to basal cell carcinoma (2014)
Basal cell carcinomas (BCCs) are the most frequent human cancer. Over 90% of all BCCs have a mutation in patched homologue 1 (PTCH 1) or smoothened (SMO), two conducting proteins of the Hedgehog (Hh ... [more ▼]
Basal cell carcinomas (BCCs) are the most frequent human cancer. Over 90% of all BCCs have a mutation in patched homologue 1 (PTCH 1) or smoothened (SMO), two conducting proteins of the Hedgehog (Hh) pathway. They rarely progress deeply and metastasize; however, if they do, these advanced BCC become amenable to treatment by inhibiting the Hedgehog and the P13K–mTOR pathways. Such innovative drugs include vismodegib, cyclopamine, itraconazole, everolimus and a few other agents that are in early clinical development. [less ▲]Detailed reference viewed: 82 (9 ULg)
Cyanoacrylate skin surface / follicular stripping.
PIERARD, Gérald ; Franchimont, Claudine ; Delvenne, Philippe et al
in Berardesca, Enzo; Wilhelm, KP; Maibach, H.I. (Eds.) Non invasive diagnostic techniques in clinical dermatology. (2014)Detailed reference viewed: 30 (14 ULg)
Development of anti-HPV lipoplexes formulations for the treatment of cervical cancer
Lechanteur, Anna ; Furst, Tania ; Evrard, Brigitte et al
Poster (2013, December 05)Detailed reference viewed: 15 (7 ULg)
Development of anti-HPV lipoplexes for the treatment of cervical cancer
Lechanteur, Anna ; Furst, Tania ; Evrard, Brigitte et al
Conference (2013, December 03)Detailed reference viewed: 13 (5 ULg)
Implication of plasmacytoid dendritic cells in the malignant transformation of cervical epithelial metaplasia
Demoulin, Stéphanie ; Somja, Joan ; Herfs, Michael et al
Poster (2013, December)Detailed reference viewed: 19 (2 ULg)