Altered alpha-defensin 5 expression in cervical squamocolumnar junction: implication in the formation of a viral/tumour-permissive microenvironment.
Hubert, Pascale ; ; et al
in The Journal of pathology (2014)
Human papillomavirus (HPV) infection, particularly type 16, is causally associated with cancer of the uterine cervix, which mainly develops at the squamocolumnar (SC) junction. The progression of cervical ... [more ▼]
Human papillomavirus (HPV) infection, particularly type 16, is causally associated with cancer of the uterine cervix, which mainly develops at the squamocolumnar (SC) junction. The progression of cervical HPV infections into (pre)neoplastic lesions suggests that viral antigens are not adequately recognized by innate immunity or presented to the adaptive immune system. Members of the defensin family have recently been found to inhibit viral and bacterial pathogens, to stimulate the migration of immune cells and to play a role in anticancer responses. In the present study, we focused on the poorly characterized human alpha-defensin 5 (HD-5) and its possible role in these processes. We showed that HD-5 was able to prevent HPV virion entry into cervical keratinocytes and to influence adaptive immunity. Indeed, this peptide specifically induced the chemoattraction and proliferation of both activated T lymphocytes and immature dendritic cells in a CCR2/CCR6-dependent manner and stimulated the infiltration of these professional antigen-presenting cells in a (pre)neoplastic epithelium transplanted in vivo in immunodeficient mice. No chemotactic effect was observed with plasmacytoid dendritic cells, macrophages or natural killer cells. Proliferative and angiogenic effects of HD-5 were also assessed in vitro and in vivo. However there was a striking regional disparity in expression of HD-5, being prominent in ectocervical, vaginal and vulvar neoplasia, while absent, or nearly so, in the cervical SC junction. Taken together, these results suggest one possible explanation for why the SC junction is uniquely vulnerable to both high-risk HPV infection (via reduced HD-5 expression and viral entry) and progression of neoplasia (via altered cell-mediated immune responses and altered microenvironment). Copyright (c) 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. [less ▲]Detailed reference viewed: 15 (3 ULg)
Cyanoacrylate skin surface strippings and the 3S-Bioket advent in tropical dermatology. A look from Liège.
PIERARD, Gérald ; Franchimont, Claudine ; PAQUET, Philippe et al
in Scientific World Journal (The) (2014), 2014
In the dermatopathology field, some simple available laboratory tests require minimum equipment for establishing a diagnosis. Among them, the cyanoacrylate skin surface stripping (CSSS), formerly named ... [more ▼]
In the dermatopathology field, some simple available laboratory tests require minimum equipment for establishing a diagnosis. Among them, the cyanoacrylate skin surface stripping (CSSS), formerly named skin surface biopsy or follicular biopsy, represents a convenient low cost procedure. It is a minimally invasive method collecting a continuous sheet of stratum corneum and horny follicular casts. In the vast majority of cases, it is painless and is unassociated with adverse events. CSSS can be performed in subjects of any age. The method has a number of applications in diagnostic dermatopathology and cosmetology, as well as in experimental dermatology settings. A series of derived analytic procedures include xerosis grading, comedometry, corneofungimetry, corneodynamics of stratum corneum renewal, corneomelametry, corneosurfametry, and corneoxenometry. [less ▲]Detailed reference viewed: 10 (2 ULg)
Tissue Proteomics for the Next Decade? Towards a Molecular Dimension in Histology
Longuespée, Rémi ; ; et al
in OMICS : A Journal of Integrative Biology (2014)Detailed reference viewed: 14 (3 ULg)
La dermatoporose, un "vintage" de l'atrophodermie et de la "peau transparente".
FRANCHIMONT, Claudine ; ; Hermanns-Lê, Trinh et al
in Revue Médicale de Liège (2014), 69
Le vieillissement de la peau se marque à terme par une atrophodermie, parfois sévère. La corticothérapie, qu’elle soit systémique ou topique, exerce un effet atrophiant qui copie l’effet du grand âge. Cet ... [more ▼]
Le vieillissement de la peau se marque à terme par une atrophodermie, parfois sévère. La corticothérapie, qu’elle soit systémique ou topique, exerce un effet atrophiant qui copie l’effet du grand âge. Cet état d’atrophodermie a été décrit comme la «peau transparente» il y a une quarantaine d’années, et il a reçu plus récemment la nouvelle dénomination de dermatoporose. Il est connu de longue date que cet état entraîne des conséquences cliniques parfois majeures qui conduisent à des lésions diverses sous forme de purpura de Bateman, de cicatrices stellaires, et de plaies faisant suite à des traumatismes mineurs. [less ▲]Detailed reference viewed: 14 (0 ULg)
Gynecologic and obstetric impact of the Ehlers-Danlos syndrome: clues from scrutinizing dermal ultrastructural alterations.
Hermanns-Lê, Trinh ; PIERARD, Gérald ; FRANCHIMONT, Claudine et al
in Gynecology (2014), 2(1),
Background: The Ehlers-Danlos syndrome (EDS) represents a heterogenous group of heritable connective tissue disorders chiefly characterized by joint laxity, increased skin distensibility and connective ... [more ▼]
Background: The Ehlers-Danlos syndrome (EDS) represents a heterogenous group of heritable connective tissue disorders chiefly characterized by joint laxity, increased skin distensibility and connective tissue fragility. Each EDS type corresponds to a multisystemic disorder with widespread implications. Physicians evoke the EDS diagnosis in presence of some typical cutaneous signs, such as atrophic scars and frequent hematomas. Methods: To revisit the transmission electron microscopy support from skin biopsies when screening EDS in gynecology-obstetrics. Results: EDS women commonly suffer from a series of gynecologic and obstetric disorders. Dermal ultrastructural changes are keynote features suggesting some EDS types. Conclusion: Gene mutations are mostly identified in the classic and vascular EDS types, but only in sporadic cases of the hypermobile EDS type which apparently represents the most frequent type. Dermal ultrastructural changes represent relevant diagnostic clues for the practitioner. Preconception counseling about possible complications is welcome. Management requires precise diagnosis of the EDS type. [less ▲]Detailed reference viewed: 28 (2 ULg)
Effect of N-acetylcysteine combined with infliximab on toxic epidermal necrolysis. A proof-of-concept study.
PAQUET, Philippe ; ; ROUSSEAU, Anne-Françoise et al
in Burns : Journal of the International Society for Burn Injuries (2014)Detailed reference viewed: 19 (3 ULg)
Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction.
Herfs, Michael ; SOMJA, Joan ; et al
in International journal of cancer. Journal international du cancer (2014)
Recent studies have identified a putative cell of origin for cervical intraepithelial neoplasia (CIN) and cervical cancer at the squamo-columnar junction (SCJ) and suggest that these cells may not ... [more ▼]
Recent studies have identified a putative cell of origin for cervical intraepithelial neoplasia (CIN) and cervical cancer at the squamo-columnar junction (SCJ) and suggest that these cells may not regenerate following excision (LEEP). This study addressed the impact of SCJ excision on the temporal dynamics, histologic and viral (HPV) characteristics of recurrent CIN. One hundred thirty one consecutive patients treated by excision and attending follow-up visits were enrolled. We compared recurrent and initial CIN with attention to excision margins, timing of recurrence, CIN grade, HPV types, p16 immunophenotype, and SCJ immunophenotype. During the follow-up period (up to four years), sixteen (12.2%) recurrences were identified. Four (25%) were identified at the first follow-up visit, closely resembled the initial CIN 2/3 in grade and HPV type, and were typically SCJ marker positive [SCJ(+)], suggesting non-excised (residual) disease. Twelve (75%) manifested after the first postoperative visit and all were in the ectocervix or in mature metaplastic epithelium. All of the 12 delayed recurrences were classified as CIN 1 and were SCJ (-). Nine of 11 SCJ (-) recurrences (82%) followed regressed spontaneously. Taken together, these results show that new lesions developing from any HPV infection are delayed and occur within the ectocervix or metaplastic epithelium. This dramatically lower risk of CIN 2/3 following successful SCJ excision suggests that removal of the SCJ could be a critical variable in reducing the risk of subsequent CIN 2/3 and cervical cancer. (c) 2014 Wiley Periodicals, Inc. [less ▲]Detailed reference viewed: 26 (1 ULg)
Le point de rosée en berne et la couche cornée hivernale.
PIERARD, Gérald ; FRANCHIMONT, Claudine ; Delvenne, Philippe
in Revue Médicale de Liège (2014), 69(2), 68-71
The frontier between the human body and the environment is largely represented by the stratum corneum. It is obvious that the skin aspect on some body sites appears dry and rough in some predisposed ... [more ▼]
The frontier between the human body and the environment is largely represented by the stratum corneum. It is obvious that the skin aspect on some body sites appears dry and rough in some predisposed individuals during winter season. The reductions in the outdoor temperature, ambient moisture and dew point are the main physical factors involved in this process. We present the aspects seen under ultraviolet light and scanning electron microscopy. Keywords : Stratum corneum - Dew point [less ▲]Detailed reference viewed: 13 (3 ULg)
Identification of a microRNA landscape targeting the PI3K/Akt signaling pathway in inflammation-induced colorectal carcinogenesis
JOSSE, Claire ; Bouznad, Nassim ; Geurts, Pierre et al
in American Journal of Physiology - Gastrointestinal and Liver Physiology (2014), 306
Inflammation can contribute to tumor formation; however, markers that predict progression are still lacking. In the present study, the well-established azoxymethane (AOM)/dextran sulfate sodium (DSS ... [more ▼]
Inflammation can contribute to tumor formation; however, markers that predict progression are still lacking. In the present study, the well-established azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced mouse model of colitis-associated cancer was used to analyze microRNA (miRNA) modulation accompanying inflammation-induced tumor development and to determine whether inflammation-triggered miRNA alterations affect the expression of genes or pathways involved in cancer. A miRNA microarray experiment was performed to establish miRNA expression profiles in mouse colon at early and late time points during inflammation and/or tumor growth. Chronic inflammation and carcinogenesis were associated with distinct changes in miRNA expression. Nevertheless, prediction algorithms of miRNA-mRNA interactions and computational analyses based on ranked miRNA lists consistently identified putative target genes that play essential roles in tumor growth or that belong to key carcinogenesis-related signaling pathways. We identified PI3K/Akt and the insulin growth factor-1 (IGF-1) as major pathways being affected in the AOM/DSS model. DSS-induced chronic inflammation downregulates miR-133a and miR-143/145, which is reportedly associated with human colorectal cancer and PI3K/Akt activation. Accordingly, conditioned medium from inflammatory cells decreases the expression of these miRNA in colorectal adenocarcinoma Caco-2 cells. Overexpression of miR-223, one of the main miRNA showing strong upregulation during AOM/DSS tumor growth, inhibited Akt phosphorylation and IGF-1R expression in these cells. Cell sorting from mouse colons delineated distinct miRNA expression patterns in epithelial and myeloid cells during the periods preceding and spanning tumor growth. Hence, cell-type-specific miRNA dysregulation and subsequent PI3K/Akt activation may be involved in the transition from intestinal inflammation to cancer. [less ▲]Detailed reference viewed: 44 (9 ULg)
Cervical cancer and HPV: Awareness and vaccine acceptability among parents in Morocco.
; ; et al
in Vaccine (2014), 32(3), 409-16
Cervical cancer is a major public health concern in Morocco where it represents the second most common and lethal cancer in women. Human papillomavirus (HPV) vaccines have been licensed in Morocco since ... [more ▼]
Cervical cancer is a major public health concern in Morocco where it represents the second most common and lethal cancer in women. Human papillomavirus (HPV) vaccines have been licensed in Morocco since 2008 but there are no available data on their acceptability. This study aimed to assess awareness of HPV and the vaccine, and to identify factors associated with acceptability of the vaccine among parents in Morocco. We carried out a questionnaire-based survey using face-to-face interviews in a sample of 852 parents (670 mothers and 182 fathers) with at least one unmarried daughter </=26 years. We collected data within public and private health centres and clinics in four regions in Morocco between July and August 2012. The main outcome measure was parental acceptability of the HPV vaccine for their daughter(s). Responses revealed very low awareness of HPV infection (4.7%) and the HPV vaccine (14.3%). None of the participants had vaccinated their daughter(s) against HPV and vaccine acceptability was low among mothers (32%) and fathers (45%). Higher education and income, previous awareness of the HPV vaccine and endorsement of the belief that a recommendation from the Ministry of Health or a doctor to have the vaccine would be encouraging, were associated with mothers' HPV vaccine acceptability. Non-acceptability among mothers was associated with having more than two daughters, believing the vaccine was expensive, lack of information and believing that whatever happens to an individual's health is God's will. The only factor associated with the fathers' acceptability of the vaccine was the cost of the vaccine. Increasing HPV and HPV vaccine awareness through educational campaigns, along with active recommendation by physicians and a publically funded vaccination programme could increase parental acceptability of the HPV vaccine in Morocco. [less ▲]Detailed reference viewed: 32 (2 ULg)
A Clinical and Pathological Overview of Vulvar Condyloma Acuminatum, Intraepithelial Neoplasia and Squamous Cell Carcinoma
LEONARD, Boris ; Kridelka, Frédéric ; DELBECQUE, Katty et al
in BioMed Research International (2014)Detailed reference viewed: 34 (11 ULg)
Hedgehog- and mTOR-targeted therapies for advanced basal cell carcinomas.
FRANCHIMONT, Claudine ; Hermanns-Lê, Trinh ; PAQUET, Philippe et al
in Current approaches to basal cell carcinoma (2014)
Basal cell carcinomas (BCCs) are the most frequent human cancer. Over 90% of all BCCs have a mutation in patched homologue 1 (PTCH 1) or smoothened (SMO), two conducting proteins of the Hedgehog (Hh ... [more ▼]
Basal cell carcinomas (BCCs) are the most frequent human cancer. Over 90% of all BCCs have a mutation in patched homologue 1 (PTCH 1) or smoothened (SMO), two conducting proteins of the Hedgehog (Hh) pathway. They rarely progress deeply and metastasize; however, if they do, these advanced BCC become amenable to treatment by inhibiting the Hedgehog and the P13K–mTOR pathways. Such innovative drugs include vismodegib, cyclopamine, itraconazole, everolimus and a few other agents that are in early clinical development. [less ▲]Detailed reference viewed: 53 (8 ULg)
Cyanoacrylate skin surface / follicular stripping.
PIERARD, Gérald ; Franchimont, Claudine ; Delvenne, Philippe et al
in Berardesca, Enzo; Wilhelm, KP; Maibach, H.I. (Eds.) Non invasive diagnostic techniques in clinical dermatology. (2014)Detailed reference viewed: 25 (10 ULg)
Development of anti-HPV lipoplexes formulations for the treatment of cervical cancer
Lechanteur, Anna ; Furst, Tania ; Evrard, Brigitte et al
Poster (2013, December 05)Detailed reference viewed: 15 (7 ULg)
Development of anti-HPV lipoplexes for the treatment of cervical cancer
Lechanteur, Anna ; Furst, Tania ; Evrard, Brigitte et al
Conference (2013, December 03)Detailed reference viewed: 13 (5 ULg)
Implication of plasmacytoid dendritic cells in the malignant transformation of cervical epithelial metaplasia
Demoulin, Stéphanie ; Somja, Joan ; Herfs, Michael et al
Poster (2013, December)Detailed reference viewed: 19 (2 ULg)
La cascade des MAP kinases : traitements ciblés en cancérologie cutanée.
PIERARD, Gérald ; Franchimont, Claudine ; LEONARD, Boris et al
in Revue Médicale de Liège (2013), 68(12), 650-654
Résumé : Les «Mitogen-Activated Protein Kinases» (MAPK) forment un ensemble coordonné de protéines cellulaires impliquées dans la prolifération, la différenciation, la migration et l’apoptose des cellules ... [more ▼]
Résumé : Les «Mitogen-Activated Protein Kinases» (MAPK) forment un ensemble coordonné de protéines cellulaires impliquées dans la prolifération, la différenciation, la migration et l’apoptose des cellules. Elles sont particulièrement activées, par certaines cytokines, hormones, le stress environnemental, ainsi que dans des cancers cutanés. Des agents pharmacologiques ciblés contre la cascade MAPK révolutionnent actuellement la cancérologie cutanée, du moins dans une phase transitoire du processus métastatique. Des traitements combinés sont susceptibles d’améliorer la survie médiane. [less ▲]Detailed reference viewed: 8 (4 ULg)