Showing results 61 to 79 of 79
  Tolerance to Osmotic Shocks in Rats Kidney Cortex and MedullaGilles, Raymond  ; Compère, Philippe ; et alin Tissue & Cell (1995), 27(6), 667-77 Kidney medulla cells of mammals have to cope with large changes in environmental osmolarity, a challenge most other mammalian cells never have to experience. In these last cells, application of osmotic ... [more ▼] Kidney medulla cells of mammals have to cope with large changes in environmental osmolarity, a challenge most other mammalian cells never have to experience. In these last cells, application of osmotic shocks induces dramatic modifications in chromatin organization. The present paper reports on the changes of medulla cell chromatin in situ, in rat kidney slices submitted to osmotic challenges and in vitro, on preparations of extracted chromatin submitted to changes in environmental ion concentrations. Our results show that the chromatin of kidney medulla cells: (1) does not behave differently from the other mammalian chromatins when submitted in situ or in vitro to osmotic challenges; (2) presents in vitro physico-chemical characteristics similar to those of the other mammalian chromatins; and (3) is protected in vitro, as the other mammalian chromatins, from the disrupting effects of increases in inorganic ion concentrations by different compensatory organic solutes. The ability of kidney medulla cells to adapt to large increases in osmolarity could thus be related to a rapid control of the level of such compounds rather than to some rather specific, intrinsic molecular adaptations of macromolecules. [less ▲] Detailed reference viewed: 8 (1 ULg)Immunogold Labelling of Fatty Acyl ChainsCompère, Philippe ; ; Goffinet, Gerhard et alin Journal of Immunological Methods (1995), 181(2), 201-9 For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either ... [more ▼] For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either directly in transmission electron microscopy or in light microscopy after silver enhancement. Good recognition of the fatty acyl chain was obtained even after treatment of the antigen coat with various cross-linking fixatives used for electron microscopy, i.e. formaldehyde, glutaraldehyde and osmium tetroxide. [less ▲] Detailed reference viewed: 4 (2 ULg)Spawning stimuli and paternity analysis in the European barbels.Poncin, Pascal ; Compère, Philippe ; et alPoster (1995) Cytochemical demonstration of acid mucopolysaccharides in the epicuticular surface coat of the crab Carcinus maenas (Crustacea, Decapoda)Compère, Philippe ; Goffinet, Gerhard in Belgian Journal of Zoology (1995), 125 Detailed reference viewed: 6 (1 ULg)Fine structure and morphogenesis of the sclerite epicuticle in the Atlantic shore crab Carcinus maenasCompère, Philippe in Tissue & Cell (1995), 27 Detailed reference viewed: 1 (1 ULg)Fine structure and morphogenesis of the sclerite epicuticle in the Atlantic shore crab Carcinus maenasCompère, Philippe in Tissue & Cell (1995), 27 Detailed reference viewed: 2 (1 ULg)Effect of high osmolarity acclimation on tolerance to hyperosmotic shocks in L929 cultured cells.Gilles, Raymond ; ; Compère, Philippe et alin Tissue & Cell (1995), 27(6), 679-687 Application of abrupt, large hyperosmotic shocks induces in L929 cultured cells changes similar to those previously described in other cell types, notably a hypercondensation of the nuclear chromatin ... [more ▼] Application of abrupt, large hyperosmotic shocks induces in L929 cultured cells changes similar to those previously described in other cell types, notably a hypercondensation of the nuclear chromatin. This paper shows that; 1) this phenomenon is concomitant with a complete disappearance of deoxyribonucleic acid, as visualized by immunogold labelling, from the nucleoplasmic spaces; 2) acclimation to high osmolarities (600 mOsm) by addition to the culture medium of NaCl, sorbitol or proline protects the cells from these effects, which appear to be largely attenuated-acclimated cells also survive much better to the osmotic shock than do control cells and; 3) the best protection seems to be provided by sorbitol and NaCl. Proline acclimation is less effective. These effects are discussed in terms of increased tolerance to NaCl load induced at the level of different macromolecules by so-called 'compensatory' organic compounds. [less ▲] Detailed reference viewed: 28 (2 ULg)Utrastructural organisation of epidermal glands in the integument of the pycnogonids Nymphon gracile Leach 1814 and Achelia longipes Hodges 1864 and Pycnogonum littorale Ström 1762 (Chelicerata, Pycnogonida); Compère, Philippe ; Bussers, Jean-Claude et alin Belgian Journal of Zoology (1995), 125 Detailed reference viewed: 6 (3 ULg)Immunogold labelling of fatty acyl chainsCompère, Philippe ; ; Goffinet, Gerhard et alin Journal of Immunological Methods (1995), 181 Detailed reference viewed: 3 (1 ULg)A screening technique to isolate bacterial strains producing high molecular weight bacteriocins; Destain, Jean-Pierre ; Compère, Philippe et alin Biotechnology Techniques (1994), 8 Detailed reference viewed: 11 (3 ULg)Ultrastructural location of calcium and magnesium during mineralization of the shore crab cuticle, as determined by the K-pyroantimonate method and X-ray microanalysisCompère, Philippe ; ; Goffinet, Gerhard in Cell & Tissue Research (1993), 274 Detailed reference viewed: 8 (1 ULg)Ultrastructural changes in the gill epithelium of the green crab Carcinus maenas in relation to the external salinityCompère, Philippe ; Wanson, Sonia ; Pequeux, André et alin Tissue & Cell (1989), 21 Detailed reference viewed: 20 (6 ULg)Ultrastructural shape and three-dimensional organization of the intracuticular canal systems in the mineralized cuticule of the green crab Carcinus maenasCompère, Philippe ; Goffinet, Gerhard in Tissue & Cell (1987), 21 Detailed reference viewed: 7 (2 ULg)Aspects ultrastructuraux et fonctionnels de diverses régions cuticulaires non minéralisées d'un crustacé décapode, Carcinus maenasCompère, Philippe ; Goffinet, Gerhard in Annales de la Société Royale Zoologique de Belgique (1987), 117 Detailed reference viewed: 7 (2 ULg)Elaboration and ultrastructural changes of the pore canal system in the mineralized cuticle of Carcinus maenas during the molting cycleCompère, Philippe ; Goffinet, Gerhard in Tissue & Cell (1987), 19 Detailed reference viewed: 6 (1 ULg)Structure, synthèse et dégradation des chitinoprotéines de la cuticule des Crustacés décapodes; Compère, Philippe ; Goffinet, Gerhard in Bollettino Zoologica (1986), 53 Detailed reference viewed: 9 (1 ULg)Experimental evidence of the role of thiamine in nerve conduction as determined by HPLC using pre and post column derivatization techniquesBettendorff, Lucien ; ; et alPoster (1984, February 25) Detailed reference viewed: 10 (0 ULg)Experimental evidence of the role of thiamine in nerve conduction as determined by HPLC using pre- and post-column derivatization techniquesBettendorff, Lucien ; ; et alin Archives Internationales de Physiologie et de Biochimie (1984), 92 Detailed reference viewed: 10 (0 ULg) |
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