References of "Collignon, Marie-Laure"
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See detailEstimation of the turbulent kinetic energy dissipation in a culture cell stirred tank bioreactor
Delafosse, Angélique ULg; Collignon, Marie-Laure ULg; Dossin, Denis et al

in Chemical Engineering Science (2011), 66(8), 1728-1737

The turbulent dissipation rate is a key parameter in stirred tanks and its local values may have a strong influence on the performance of many processes. However, the local dissipation rate estimation is ... [more ▼]

The turbulent dissipation rate is a key parameter in stirred tanks and its local values may have a strong influence on the performance of many processes. However, the local dissipation rate estimation is far from easy in a stirred tank, especially near the impeller discharge where maximum values are encountered. The aim of this work is to estimate the dissipation rate in a vessel used for animal-cell cultures and stirred with a down-pumping axial impeller (Mixel TTP) from velocity fields measured by 2D-PIV. Special attention is paid to the assumptions necessary to estimate the dissipation rate from 2D measurements and to the influence of measurement spatial resolution on the estimated values. The analysis of isotropy ratios measured on vertical, horizontal and tangential planes shows that the turbulence in the impeller discharge is far from isotropic. Isotropy assumptions classically used to estimate the dissipation rate from 2D measurements may thus lead to erroneous values. Based on the measured isotropy ratios, a new relationship is proposed to estimate the dissipation rate in the impeller discharge. This relationship is then used to estimate the dissipation rate on a vertical plane located in the impeller discharge zone. In order to analyze the influence of the measurement spatial resolution on the estimated values of the dissipation, a total of 12 spatial resolutions are tested. Results show that if the spatial resolution is divided by a factor 2, the dissipation rate increases by 220%. For the smallest spatial resolution value used, the maximum dissipation rate estimated is 50 times higher than the mean overall dissipation rate and the corresponding minimum value of the Kolmogorov scale is nearly 3 times smaller than the Kolmogorov scale computed from the mean overall dissipation rate. [less ▲]

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See detailAXIAL IMPELLER SELECTION FOR ANCHORAGE DEPENDENT ANIMAL CELL CULTURE IN STIRRED BIOREACTORS: METHODOLOGY BASED ON THE IMPELLER COMPARISON AT JUST-SUSPENDED SPEED OF ROTATION
Collignon, Marie-Laure ULg; Delafosse, Angélique ULg; Crine, Michel ULg et al

in Chemical Engineering Science (2010), 65(22), 5929-5941

Animal cells, which are nowadays essential for the industrial production of proteinic compounds, are commonly cultivated inside stirred tank bioreactors. In case of anchorage dependent cells, they are ... [more ▼]

Animal cells, which are nowadays essential for the industrial production of proteinic compounds, are commonly cultivated inside stirred tank bioreactors. In case of anchorage dependent cells, they are usually fixed on microcarriers. The choice of agitation conditions (impeller type, rotational speed…) in this type of process is not an easy task as it has to fulfil three potentially conflicting goals: (1) maintaining microcarriers in complete suspension, (2) homogenizing the culture medium, and (3) limiting mechanical constraints generated by the hydrodynamics on the cells. The aim of this study is to present an original methodology to select the most appropriate axial impeller for this specific application. Seven propellers are preselected on basis of their characteristics available in the literature. Instead of comparing impellers at a given rotational speed or a given power input, they are compared at their respective minimum impeller rotational speed that leads to a complete microcarrier suspension, i.e. at their respective just-suspended speed Njs. They are then compared at higher rotational speeds N, expressed as multiples of Njs. The impeller classification is based on the intensity of mechanical constraints they produced, evaluated from: (1) the macro-shear rate quantified by the spatial derivative of time average velocity fields measured by P.I.V, (2) the micro-shear rate characterized by the ratio between the microcarrier diameter to the average Kolmogorov scale computed from power input measurements, and (3) the impact of microcarrier collisions on cells described via the Turbulent Collision Severity index also computed from power input measurements. Results show that the 125 mm diameter TTP impeller (Mixel) and the 150 mm diameter Elephant Ear impeller (Applikon) produce the smallest mechanical constraints at their just-suspended speed (50 rpm and 20 rpm, respectively). Moreover, the mechanical constraints they produce increase more slowly with the N/Njs ratio than the mechanical constraints produced by other impellers. These propellers are thus even more advantageous if rotational speeds higher than the just-suspended speed have to be used. [less ▲]

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See detailDevelopment of a compartment model based on CFD simulations for mixing description in bioreactors
Delafosse, Angélique ULg; Delvigne, Frank ULg; Collignon, Marie-Laure ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(2), 517-522

Understanding and modelling the complex interactions between biological reaction and hydrodynamics is a key problem when dealing with bioprocesses. It is fundamental to be able to accurately predict the ... [more ▼]

Understanding and modelling the complex interactions between biological reaction and hydrodynamics is a key problem when dealing with bioprocesses. It is fundamental to be able to accurately predict the hydrodynamics behaviour of bioreactors of different size and its interaction with the biological reaction. CFD can provide detailed modelling about hydrodynamics and mixing. However, it is computationally intensive, especially when reactions are taken into account. Another way to predict hydrodynamics is the use of "Compartment" or "Multi-zone" model which are much less demanding in computation time than CFD. However, compartments and fluxes between them are often defined by considering global quantities not representative of the flow. To overcome the limitations of these two methods, a solution is to combine compartment modelling and CFD simulations. Therefore, the aim of this study is to develop a methodology in order to propose a compartment model based on CFD simulations of a bioreactor. The flow rate between two compartments can be easily computed from the velocity fields obtained by CFD. The difficulty lies in the definition of the zones in such a way they can be considered as perfectly mixed. The creation of the model compartments from CFD cells can be achieved manually or automatically. The manual zoning consists in aggregating CFD cells according to the user's wish. The automatic zoning defines compartments as regions within which the value of one or several properties are uniform with respect to a given tolerance. Both manual and automatic zoning methods have been developed and compared by simulating the mixing of an inert scalar. For the automatic zoning, several algorithms and different flow properties have been tested as criteria for the compartment creation. [less ▲]

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See detailQuality of mixing in a stirred bioreactor used for animal cells culture: heterogeneities in a lab scale bioreactor and evolution of mixing time with scale up
Collignon, Marie-Laure ULg; Dossin, Denis; Delafosse, Angélique ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14

Animal cells are industrially cultivated inside stirred bioreactors to produce proteinic compounds. Due to the use of mild agitation conditions in order to limit mechanical constraints, the homogeneity of ... [more ▼]

Animal cells are industrially cultivated inside stirred bioreactors to produce proteinic compounds. Due to the use of mild agitation conditions in order to limit mechanical constraints, the homogeneity of the culture medium can be far from perfect. This study has therefore two objectives. 1) the global characterization of the mixing via the mixing time 2) the local description of concentration fields. The mixing time is measured by conductimetry inside 20 L, 80 L, 600L tanks. The Grenville correlation is adjusted on these experimental measurements to improve the prediction of the mixing time during the scale-up of the process. The concentration fields are visualized by the Planar Laser Induced Fluorescence (P.L.I.F) technique in the 20 L tank. This part of the study is focused on the time evolution of the maximum value of the tracer concentration inside measurement planes and of the numerical distribution of theses concentration fields. [less ▲]

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See detailEffet de la viscosité sur la structure de l'écoulement et sur le mélange dans un bioréacteur à cuve agitée utilisé en culture de cellules animales
Collignon, Marie-Laure ULg; Crine, Michel ULg; Delafosse, Angélique ULg et al

in Récents Progrès en Génie des procédés - A la croisée des sciences et des cultures, pour relever les défis industriels du XXIème siècle (2009)

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See detailEtude du mélange par PIV et PLIF dans un bioréacteur de culture cellulaire
Collignon, Marie-Laure ULg

Master's dissertation (2007)

Ce mémoire consiste en l’étude expérimentale de l’hydrodynamique et du mélange dans un bioréacteur de 20 litres de culture de cellules animales sur microporteurs. Les résultats obtenus serviront aux ... [more ▼]

Ce mémoire consiste en l’étude expérimentale de l’hydrodynamique et du mélange dans un bioréacteur de 20 litres de culture de cellules animales sur microporteurs. Les résultats obtenus serviront aux chercheurs de la société GlascoSmithKline Biologicals qui procèdent actuellement à la montée en échelle à partir d’un bioréacteur de 20 litres pour atteindre une échelle industrielle de plusieurs mètres cubes. Dès lors un grand nombre de paramètres ont été imposés tels que la géométrie du système et la gamme des vitesses de rotation du mobile et le type de celui-ci (hélice TT). Les techniques expérimentales utilisées sont la P.I.V. (Particle Image Velocimetry) et la P.L.I.F. (Planar Laser Induced Fluorescence) qui permettent d’accéder respectivement au champ de vitesses et champ de concentrations dans un plan vertical éclairé par le laser. Ce travail consiste en une étude paramétrique au cours de laquelle nous avons fait varier les différents constituants du nombre de Reynolds dans une cuve agitée : la vitesse de rotation N, la taille du mobile d et la viscosité du fluide µ. Nous avons déterminé expérimentalement les grandeurs globales suivantes : le débit et nombre de pompage, le débit et nombre de circulation, la puissance dissipée globalement et le nombre de puissance y correspondant, la hauteur théorique équivalente d’une pompe et le temps de mélange. Nous avons également caractérisé l’hydrodynamique et le mélange de manière locale : en décrivant le champ de vitesses dans le plan éclairé par le laser, en déterminant la répartition du cisaillement et de la dissipation de l’énergie dans ce même plan et en représentant le mélange par un modèle compartimenté constitué d’un réseau de cuves parfaitement mélangées mises en série. L’évolution de ces grandeurs globales et locales en fonction des paramètres du nombre de Reynolds a été analysée. Les résultats obtenus donneront des informations importantes qui aideront les chercheurs de G.S.K. BIO. à affiner leur stratégie de scale-up. Enfin, un des points cruciaux de l’opération de mélange est le maintien en suspension complète des petites billes sur lesquelles se développent les cellules qui sont appelées les microporteurs. La vitesse de rotation qui permet le maintien de cette suspension complète a été caractérisée expérimentalement et à l’aide d’un modèle qui pourra être aisément utilisé lors du processus de scale-up. [less ▲]

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