References of "Colinet, Frédéric"
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See detailEstimation des effets du gène culard sur les caractères de production laitière au sein du rameau Blanc Bleu Belge de type mixte
Colinet, Frédéric ULg; Gengler, Nicolas ULg

in 16ièmes Rencontres Recherches Ruminants, Paris, les 2 et 3 Décembre 2009 (2009, December)

Le rameau Blanc Bleu Belge de type mixte (BBm) est apprécié pour sa rusticité, sa longévité, une bonne santé ainsi que pour ses bonnes performances de reproduction. Une délétion de 11 paires de base ... [more ▼]

Le rameau Blanc Bleu Belge de type mixte (BBm) est apprécié pour sa rusticité, sa longévité, une bonne santé ainsi que pour ses bonnes performances de reproduction. Une délétion de 11 paires de base (allèle mh) au sein du gène de la myostatine engendre le syndrome d’hypertrophie musculaire (plus connu sous le nom de type culard ; d’ailleurs, cet allèle mh est souvent appelé le gène culard). Bien que cet allèle mh soit très présent au sein du rameau Blanc Bleu Belge de type viandeux, seulement 41 et 39 % des animaux BBm sont respectivement mh/mh et mh/+. Etant donné l’importance des effets du gène culard sur la conformation viandeuse des animaux et de son rôle potentiel indirect en production laitière, il nous a semblé intéressant de tenir compte de sa présence lors de l’évaluation génétique des animaux BBm. Néanmoins, il n’est pas envisageable et concevable de génotyper tous les individus de la population BBm. Dès lors, il a fallu modifier légèrement le modèle mixte utilisé pour pouvoir y incorporer ce génotype connu pour certains animaux. La méthodologie ici présentée permet l’estimation simultanée des effets polygéniques (plus connus sous le terme « valeur d’élevage », VE) et de l’effet du gène culard. [less ▲]

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See detailGenetic evaluation considering phenotypic data and limited molecular information using a novel equivalent model: Case study using effect of the mh locus on milk production in the dual-purpose Belgian Blue breed
Colinet, Frédéric ULg; Gengler, Nicolas ULg

Conference (2009, August)

The introduction of molecular information into genetic evaluation systems is currently under research. Based on an equivalent method, we developed from existing theory a new alternative strategy for the ... [more ▼]

The introduction of molecular information into genetic evaluation systems is currently under research. Based on an equivalent method, we developed from existing theory a new alternative strategy for the prediction of gene effects and especially their smooth integration into genetic evaluations. Underlying hypothesis were based on the idea that knowledge of genotypes will not affect overall additive genetic variance but only change expected values of genetic effects for animals with known genotypes. However, all animals could not be genotyped. Thus, the developed equations were modified to allow the integration of the known genotype for a portion of the population. This strategy was tested for the mh locus (responsible for the double-muscling phenotype) in dual-purpose Belgian Blue cattle. The genotype was determined for 123 bulls and 1940 cows (+/+ 19.5, mh/+ 39.3 and mh/mh 41.2%). These animals had 11,150 daughters with test-day (TD) records. The genotypes were incorporated into a modified genetic evaluation based on the current routine multi-trait multibreed test-day model used in the Walloon Region (Belgium). Data used included 12,829,309 TD records for 689,057 dairy cows in production. The pedigree file contained 1,606,024 animals (cows with TD records and ancestors). Computation of the modified mixed model equations was done solving iteratively two systems of equations, one for the polygenic effects and one for the gene effect until the relative differences in the gene solutions were below 10-5. A linear extrapolation was also used to speed up the convergence of gene effects. As expected, the mh locus exerts negative effects on milk production traits. For the first three lactations, the average estimated allelic substitution effects were -158.7 kg milk, -8.93 kg fat and -5.64 kg protein per lactation (305 days). [less ▲]

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See detailGenetic evaluation considering phenotypic data and limited molecular information using a novel equivalent model: Case study using effect of the mh locus on milk production in the dual-purpose Belgian Blue breed
Colinet, Frédéric ULg; Gengler, Nicolas ULg

in Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production (2009, August)

The introduction of molecular information into genetic evaluation systems is currently under research. Based on an equivalent method, we developed from existing theory a new alternative strategy for the ... [more ▼]

The introduction of molecular information into genetic evaluation systems is currently under research. Based on an equivalent method, we developed from existing theory a new alternative strategy for the prediction of gene effects and especially their smooth integration into genetic evaluations. Underlying hypothesis were based on the idea that knowledge of genotypes will not affect overall additive genetic variance but only change expected values of genetic effects for animals with known genotypes. However, all animals could not be genotyped. Thus, the developed equations were modified to allow the integration of the known genotype for a portion of the population. This strategy was tested for the mh locus (responsible for the double-muscling phenotype) in dual-purpose Belgian Blue cattle. The genotype was determined for 123 bulls and 1940 cows (+/+ 19.5, mh/+ 39.3 and mh/mh 41.2%). These animals had 11,150 daughters with test-day (TD) records. The genotypes were incorporated into a modified genetic evaluation based on the current routine multi-trait multibreed test-day model used in the Walloon Region (Belgium). Data used included 12,829,309 TD records for 689,057 dairy cows in production. The pedigree file contained 1,606,024 animals (cows with TD records and ancestors). Computation of the modified mixed model equations was done solving iteratively two systems of equations, one for the polygenic effects and one for the gene effect until the relative differences in the gene solutions were below 10-5. A linear extrapolation was also used to speed up the convergence of gene effects. As expected, the mh locus exerts negative effects on milk production traits. For the first three lactations, the average estimated allelic substitution effects were -158.7 kg milk, -8.93 kg fat and -5.64 kg protein per lactation (305 days). [less ▲]

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See detailBLUESEL - an INTERREG France-Wallonie-Vlaanderen project aiming at the conservation and the use of the genetic heritage of the dual-purpose Blue Breeds in Belgium and Northern France
Colinet, Frédéric ULg

Poster (2009, August)

Dual-purpose Belgian Blue and North Blue cattle are mainly located on both sides of the border between France and Belgium. Even if these Belgian and French Blue breeds are related because of their common ... [more ▼]

Dual-purpose Belgian Blue and North Blue cattle are mainly located on both sides of the border between France and Belgium. Even if these Belgian and French Blue breeds are related because of their common ancestors in the former Mid and High Belgium cattle, these breeds diverged slightly under differentiated selection objectives in both countries. Within the BLUESEL project, a first aim consists to create a working group cross-border which will develop common guidelines for selection of bull dams and elite-matings for this dual-purpose Blue Breeds. This working group will create and help to conserve a common pool of bulls available for breeding in both countries. The project will also develop tools to harmonize the collection of phenotypic data (milk production and morphology). A joint genetic evaluation for production traits will be developed, adapted to the specifities of these breeds and integrating data provided by both countries. Others objectives of BLUESEL are the implementation of a technical and economical guidance of these farms and the improvement of profitability of farms through advice on management and on improvement of livestock. The valorisation of these breeds through the development of new and specific products (e.g. cheese products) is another objective. In summary, the whole project should contribute maintaining biodiversity in this cross-border region through conservation and use of animals naturally adapted. The BLUESEL project was launched in July 2008. It is conceived for four years and is supported by the European Union, the Walloon Region, the Nord-Pas-de Calais Region and the General Council of Department of Nord. [less ▲]

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See detailBLUESEL - an INTERREG France-Wallonie-Vlaanderen project aiming at the conservation and the use of the genetic heritage of the dual-purpose Blue Breeds in Belgium and Northern France
Colinet, Frédéric ULg

in Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production (2009, August)

Dual-purpose Belgian Blue and North Blue cattle are mainly located on both sides of the border between France and Belgium. Even if these Belgian and French Blue breeds are related because of their common ... [more ▼]

Dual-purpose Belgian Blue and North Blue cattle are mainly located on both sides of the border between France and Belgium. Even if these Belgian and French Blue breeds are related because of their common ancestors in the former Mid and High Belgium cattle, these breeds diverged slightly under differentiated selection objectives in both countries. Within the BLUESEL project, a first aim consists to create a working group cross-border which will develop common guidelines for selection of bull dams and elite-matings for this dual-purpose Blue Breeds. This working group will create and help to conserve a common pool of bulls available for breeding in both countries. The project will also develop tools to harmonize the collection of phenotypic data (milk production and morphology). A joint genetic evaluation for production traits will be developed, adapted to the specifities of these breeds and integrating data provided by both countries. Others objectives of BLUESEL are the implementation of a technical and economical guidance of these farms and the improvement of profitability of farms through advice on management and on improvement of livestock. The valorisation of these breeds through the development of new and specific products (e.g. cheese products) is another objective. In summary, the whole project should contribute maintaining biodiversity in this cross-border region through conservation and use of animals naturally adapted. The BLUESEL project was launched in July 2008. It is conceived for four years and is supported by the European Union, the Walloon Region, the Nord-Pas-de Calais Region and the General Council of Department of Nord. [less ▲]

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See detailEquivalent mixed model for joint genetic evaluation considering molecular and phenotypic information
Gengler, Nicolas ULg; Colinet, Frédéric ULg

Conference (2009, July)

Currently efforts are underway to introduce molecular information into genetic evaluation systems. A particular situation is genomic selection however simpler cases exists where major genes are known and ... [more ▼]

Currently efforts are underway to introduce molecular information into genetic evaluation systems. A particular situation is genomic selection however simpler cases exists where major genes are known and used by breeders. A new alternative strategy for the prediction of gene effects and especially their smooth integration into genetic evaluations based on an equivalent method was developed from existing theory. Underlying hypothesis were based on the idea that knowledge of genotypes will not affect overall additive genetic variance but only change expected values of genetic effects for animals with known genotypes. The developed equations were modified to allow that not all animals were genotyped. As the underlying mixed model is open a very large range of models can be used in situations including random regression models, multipletrait, maternal effects and multiple-across-country-evaluation models. Computations involved successive solving of two mixed models, with the use of an linear extrapolation to speed up convergence of gene effects. The method was tested for several known major genes and QTL, e.g. for the mh gene in the dual-purpose Belgian Blue population in Belgium. Modifications of the method could also be developed to be useful in the context of genomic selection. [less ▲]

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See detailEquivalent mixed model for joint genetic evaluation considering molecular and phenotypic information
Gengler, Nicolas ULg; Colinet, Frédéric ULg

in Journal of Dairy Science (2009), 92(E-suppl.1), 315

Currently efforts are underway to introduce molecular information into genetic evaluation systems. A particular situation is genomic selection however simpler cases exists where major genes are known and ... [more ▼]

Currently efforts are underway to introduce molecular information into genetic evaluation systems. A particular situation is genomic selection however simpler cases exists where major genes are known and used by breeders. A new alternative strategy for the prediction of gene effects and especially their smooth integration into genetic evaluations based on an equivalent method was developed from existing theory. Underlying hypothesis were based on the idea that knowledge of genotypes will not affect overall additive genetic variance but only change expected values of genetic effects for animals with known genotypes. The developed equations were modified to allow that not all animals were genotyped. As the underlying mixed model is open a very large range of models can be used in situations including random regression models, multipletrait, maternal effects and multiple-across-country-evaluation models. Computations involved successive solving of two mixed models, with the use of an linear extrapolation to speed up convergence of gene effects. The method was tested for several known major genes and QTL, e.g. for the mh gene in the dual-purpose Belgian Blue population in Belgium. Modifications of the method could also be developed to be useful in the context of genomic selection. [less ▲]

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See detailGenomic location of the bovine growth hormone secretagogue receptor (Ghsr) gene and investigation of genetic polymorphism
Colinet, Frédéric ULg; Vanderick, Sylvie ULg; Charloteaux, Benoît ULg et al

in Animal Biotechnology (2009), 20(1), 28-33

The growth hormone secretagogue receptor (GHSR) is involved in the regulation of energetic homeostasis and GH secretion. In this study, the bovine GHSR gene was mapped to BTA1 between BL26 and BMS4004 ... [more ▼]

The growth hormone secretagogue receptor (GHSR) is involved in the regulation of energetic homeostasis and GH secretion. In this study, the bovine GHSR gene was mapped to BTA1 between BL26 and BMS4004. Two different bovine GHSR CDS (GHSR1a and GHSR1b) were sequenced. Six polymorphisms (five SNPs and one 3-bp indel) were also identified, three of them leading to amino acid variations L24V, D194N, and Del R242. These variations are located in the extracellular N-terminal end, the exoloop 2, and the cytoloop 3 of the receptor, respectively. [less ▲]

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See detailGenetic analysis of lactoferrin content in bovine milk
Arnould, Valérie ULg; Soyeurt, Hélène ULg; Gengler, Nicolas ULg et al

in Journal of Dairy Science (2009), 92(5), 2151-2158

Bovine lactoferrin (LF) is mainly present in milk and shows important physiological and biological functions. The aim of this study was to estimate the heritability and correlation values of LF content in ... [more ▼]

Bovine lactoferrin (LF) is mainly present in milk and shows important physiological and biological functions. The aim of this study was to estimate the heritability and correlation values of LF content in bovine milk with different economic traits as milk yield (MY), fat and protein percentages, and somatic cell score (SCS). Variance components of the studied traits were estimated by REML using a multiple-trait mixed model. The obtained heritability (0.22) for LF content predicted using mid-infrared spectrometry (pLF) suggested the possibility of animal selection based on the increase of LF content in milk. The phenotypic and genetic correlation values calculated between pLF and SCS were moderate (0.31 and 0.24, respectively). Furthermore, a preliminary study of bovine LF gene polymorphism effects was performed on the same production traits. By PCR, all exons of the LF gene were amplified and then sequenced. Three new polymorphisms were detected in exon 2, exon 11, and intron 8. We examined the effects of LF gene polymorphisms of exons 2, 4, 9, 11, and 15, and intron 8 on pLF, MY, fat and protein percentages, and SCS. The different observed effects did not reach a significant level probably because of the characteristics of the studied population. However, the results were promising, and LF may be a potential indicator of mastitis. Further studies are necessary to evaluate the effect of genetic selection based on LF content on the improvement of mastitis resistance. [less ▲]

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See detailMolecular characterization of the bovine GHRL gene
Colinet, Frédéric ULg; Portetelle, Daniel ULg; Renaville, Robert ULg

in Archiv für Tierzucht = Archives Animal Breeding (2009), 52(1), 79-84

Bovine ghrelin, a 27 amino acid pepticle, has been identified in oxyntic glands of the abomasum. It is an endogenous ligand for growth hormone secretagogue receptor and stimulates food intake and growth ... [more ▼]

Bovine ghrelin, a 27 amino acid pepticle, has been identified in oxyntic glands of the abomasum. It is an endogenous ligand for growth hormone secretagogue receptor and stimulates food intake and growth hormone secretion. The bovine GHRL gene was completely sequenced and consists of five exons and four introns. Like mouse and human GHRL genes, we found that the bovine GHRL gene also contains a first non-coding exon of 21 bp. The bovine GHRL gene codes for 116 amino acid pepticle named preproghrelin which contains the ghrelin pepticle and another pepticle similar to obestatin. Sequence analysis revealed eight polymorphisms, which are located in the non-coding sequence of the gene. [less ▲]

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See detailEtudes génétiques et immunomodulatoires de la ghréline sur les traits de production et de conformation en races bovines ainsi que sur la croissance chez le rat
Colinet, Frédéric ULg

Doctoral thesis (2008)

En production animale, notamment dans les filières bovines, il est d’un intérêt économique d’augmenter la quantité d’hormone de croissance dans la circulation sanguine. La ghréline est un peptide ... [more ▼]

En production animale, notamment dans les filières bovines, il est d’un intérêt économique d’augmenter la quantité d’hormone de croissance dans la circulation sanguine. La ghréline est un peptide principalement produit au niveau de la paroi stomacale. Ce ligand endogène au GHSR stimule la sécrétion hypophysaire de l’hormone de croissance. Peptide orexigène, la ghréline est impliquée dans les mécanismes relatifs au maintien de l’homéostasie énergétique. Dans l’optique d’améliorer les performances animales, deux approches de la ghréline ont été effectuées. La première approche consiste en l’étude des gènes bovins codant pour la ghréline (bGHRL) et son récepteur (bGHSR). Ces deux gènes ont été respectivement localisés sur BTA 22 et BTA 1. Quatorze polymorphismes ont été détectés sur ces deux gènes et trois d’entre eux affectent la structure primaire du GHSR bovin. Des associations, à différents niveaux de signification, entre certains de ces 14 sites polymorphiques et des traits de production et de conformation ont été mis en évidence au sein d’un groupe de 127 taureaux Holstein sur base de leurs descendances directes présentes en Région Wallonne. La seconde approche aborde les effets d’une immunisation passive contre la ghréline sur des rats mâles en croissance en comparaison à celles contre la leptine et la cholécystokinine. Lors d’une alimentation équilibrée, le traitement envers la ghréline sur ces rats n’a pas influencé la croissance et l’ingestion par rapport aux animaux témoins. Des effets ont été observés entre les différentes immunomodulations au niveau des paramètres de croissance, d’ingestion et endocrinologiques. Les présents résultats invitent à de nouvelles investigations des gènes bGHRL et bGHSR sur des données relatives à d’autres populations/races bovines et de l’immunomodulation de la ghréline lors de conditions d’expérimentation différentes (alimentation déséquilibrée, stade physiologique, espèce, etc.). Ces investigations pourraient être valorisées en sélection et production animale mais également en médecine tant humaine que vétérinaire. [less ▲]

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See detailVariabilité génétique des composés 'Santé' du lait prédits par la spectrométrie du moyen-infrarouge
Soyeurt, Hélène ULg; Dehareng, Frédéric; Dardenne, Pierre et al

in 13ième Carrefour des Productions Animales: L'Elevage des ruminants en question: vérités et contre-vérités (2008)

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See detailGenetic variability of lactoferrin content estimated by mid-infrared spectrometry in bovine milk
Soyeurt, Hélène ULg; Colinet, Frédéric ULg; Arnould, Valérie ULg et al

in Journal of Dairy Science (2007), 90(9), 4443-4450

The effects of lactoferrin (LF) on the immune system have already been shown by many studies. Unfortunately, the current methods used to measure LF levels in milk do not permit the study of the genetic ... [more ▼]

The effects of lactoferrin (LF) on the immune system have already been shown by many studies. Unfortunately, the current methods used to measure LF levels in milk do not permit the study of the genetic variability of lactoferrin or the performance of routine genetic evaluations. The first aim of this research was to derive a calibration equation permitting the prediction of LF in milk by mid-infrared spectrometry (MIR). The calibration with partial least squares on 69 samples showed a ratio of standard error of cross-validation to standard deviation equal to 1.98. Based on this value, the calibration equation was used to establish an LF indicator trait (predicted LF; pLF) on a large number of milk samples (n = 7,690). A subsequent study of its variability was conducted, which confirmed that stage of lactation and lactation number influence the overall pLF level. Small differences in mean pLF among 7 dairy breeds were also observed. The pLF content of Jersey milk was significantly higher than that in Holstein milk. Therefore, the choice of breed could change the expected LF level. Heritability estimated for pLF was 19.7%. The genetic and phenotypic correlations between somatic cell score and pLF were 0.04 and 0.26, respectively. As somatic cell score increases in presence of mastitis, this observation seems to indicate that pLF, or a function of observed pLF, compared with expected LF might have potential as an indicator of mastitis. The negative genetic correlation (−0.36) between milk yield and pLF could indicate an undesirable effect of selection for high milk production on the overall LF level. [less ▲]

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See detailVariation of lactoferrin content predicted by mid-infrared spectrometry (MIR)
Soyeurt, Hélène ULg; Colinet, Frédéric ULg; Arnould, Valérie ULg et al

in Book of Abstracts of the 58th Annual Meeting of the European Association for Animal Production (2007)

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See detailMapping of the bovine growth hormone secretagogue receptor (GHS-R) and polymorphism study in cattle
Colinet, Frédéric ULg; Eggen, André; Gengler, Nicolas ULg et al

Poster (2006, December)

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and ... [more ▼]

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and its subtype are abundantly located in the hypothalamus-pituitary unit, but are also distributed in other central areas and peripheral tissues. The GHS-R belongs to the G-protein coupled receptor family with seven transmembrane domain architecture. In order to determine the GHS-R gene sequence, total mRNA was extracted from abomasum and two types of GHS-R cDNA were identified. These two types are transcript variants (1a and 1b) of the same GHS-R gene. The gene encompasses two exons and a single intron. Using a 3000 Rad hybrid panel, the GHS-R gene was mapped to Bos taurus autosome 1 (BTA 1). This localization on BTA 1 agrees totally with comparative data between cattle and human since BTA 1 corresponds to part of human chromosome 3 where human GHSR is also mapped. By two-point analysis, most significantly linked marker are BL26 and BMS4031 (both LOD score : 5,66). Some studies detected different QTLs near these markers like for growth rate, carcass yield, milk portein and milk yield. In the cattle industry, it is of economical importance to increase plasma GH secretion because it is associated with faster growth, less fat stores and improved milk production. Being of economical importance and the detected QTLs near the GHS-R gene, it would be interesting to study the polymorphism on the bovine GHS-R gene. Screening for polymorphisms in the two exons on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of four single nucleotide polymorphisms (SNPs): three SNPs are in the first exon and one SNP in the second exon. In order to evaluate if GHS-R could be involved in genetic variation for growth rate, carcass yield, milk portein and milk yield, an association study between SNPs on GHS-R gene and these traits could be performed in a major cattle population. [less ▲]

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See detailIsolation of insecticidal proteins within the pea seeds (Pisum sativum L.)
Cuartero Diaz, Gaëtan; Francis, Frédéric ULg; Portetelle, Daniel ULg et al

Poster (2006, October)

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and ... [more ▼]

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and moreover those products are not competing enough. In this context, the aim of this research is to set up a biological insecticide, which is economic, with vegetal proteins resulting from alimentary industry, here the pea, Pisum sativum L.. A group of proteins, which is quite easy to highlight, is present in relatively important proportions (2%) in pea seeds, it’s the lectins class. Insecticidal effects of lectins from different organisms have already been proved. Indeed, by binding to membrane glycosyl groups of digestive tract cells, lectins can be very toxic for a lot of insects. Thus initially we focus our investigations on Pisum sativum lectins (PSL). First, PSL have been localised within the industrial process among different extraction juices. Then, a chromatography has been performed on the selected juice with FPLC technology. Although the matrix used for this chromatography, sephadex G75, is a banal bed for gel filtration, it is in this case a real combination between classical gel filtration and affinity chromatography. Indeed due the particular properties of lectins, they fixed carbonyl group of the bed and have to be eluted after the filtration part with a solution of glucose. Then the collected fractions corresponding to UV-peaks on the chromatogram were separated by electrophoresis 2D and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Secondly bioassays using artificial diets have been developed on Myzus persicae in the aim to study the aphicid effects of theses fractions with rm and LC50.These estimators show significant mortality rates but also change in the fecundity and in the development of nymph. [less ▲]

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