References of "Colige, Alain"
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See detailTranscriptional Profiling after Lipid Raft Disruption in Keratinocytes Identifies Critical Mediators of Atopic Dermatitis Pathways
Mathay, Conny; Pierre, Michael; Depiereux, Eric et al

in Journal of Investigative Dermatology (2011), 131

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See detailEvaluation of the use of VEGF111 for the treatment of tendon lesions.
Janssen, Lauriane ULg; Kaux, Jean-François ULg; Drion, Pierre ULg et al

Poster (2011, May 20)

Alterations of tendons are common pathologies resulting from repetitive or abnormal mechanical sollicitations. Very frequently lesions become chronic and may even lead to rupture. As there is no current ... [more ▼]

Alterations of tendons are common pathologies resulting from repetitive or abnormal mechanical sollicitations. Very frequently lesions become chronic and may even lead to rupture. As there is no current efficient treatment for curing this type of diseases, new therapeutic approaches are being tested and developed. Injection of platelet-rich plasma (PRP) seems to be a promising treatment by local release of growth factors. Among these factors, VEGF-A is known to induce positive effects on vascular functions and angiogenesis, and could be implicated in the healing process of tendons. Several isoforms of VEGF-A have been described in literature, including VEGF165 and 121. VEGF111 is encoded by exons 1-4 and 8a. The lack of exon 5 enables VEGF111 to resist to proteolytic degradation and the absence of exons 6 and 7 reduces its affinity for several macromolecules present on the cell surface and in the extracellular matrix. In vivo, it has been shown to be highly proangiogenic and diffusible. A 5mm defect was surgically performed in the Achilles tendon of 60 rats. Two hours after closure of the fascia and the skin, an injection within the wound was performed with PBS alone (n=30) or with PBS containing 100 ng of VEGF111 (n=30). 10 rats of each group were sacrificed at days 5, 15 and 30. The operated tendon was then carefully removed and collected for either immunohistochemical analyses or mechanical testing. At each time point, the section and the overall appearance of the repairing tendons were similar for PBS and VEGF111-injected tendon. As compared to controls, injection of VEGF111 seemed to promote a faster angiogenesis, although the number of samples was at this stage too low for performing reliable statistical analysis. Mechanical resistance to rupture of the repairing tendons was also measured. No difference between the two groups was observed after 5 or 15 days. By contrast, increased tensile strength was clearly evidenced in the VEGF-treated group after 30 days. These preliminary data seem to indicate a positive effect of a single VEGF111 injection for restoring the mechanical properties of tendons after their section. Additional experiments are planned for confirmation purposes and for further characterizing the model. It includes a “dose- response” analysis, the use of VEGF165 as an additional control and a study evaluating the effect of several injections. [less ▲]

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See detailChitosan-based nanofibers for wound dressing
Aqil, Abdelhafid ULg; Tchemtchoua Tateu, Victor ULg; Colige, Alain ULg et al

Poster (2011, May 12)

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See detailPlatelet-rich plasma and tendons healing: rat model
Kaux, Jean-François ULg; Drion, Pierre ULg; Colige, Alain ULg et al

in Biomedica Life Science Summit (2011, April 07)

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See detailInfluence sur le tissu tendino(-musculaire) du mode de contraction en entraînement : modèle animal
Kaux, Jean-François ULg; Drion, Pierre ULg; Croisier, Jean-Louis ULg et al

in Julia, Marc; Hirt, Daniel; Croisier, Jean-Louis (Eds.) et al Tendon et jonction tendino-musculaire - De la biomécanique aux applications thérapeutiques (2011)

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See detailmicroRNA-21 Exhibits Anti-Angiogenic Function by Targeting RhoB Expression in Endothelial Cells
Sabatel, Céline; Malvaux, Ludovic; Bovy, Nicolas ULg et al

Poster (2011, February)

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See detailPlatelet-rich plasma (PRP) and tendon healing: animal model
Kaux, Jean-François ULg; Drion, Pierre ULg; Renouf, Julien et al

in British Journal of Sports Medicine (2011, February), 45(2), 1

Introduction: The tendon is a tissue which does not heal easily. Recently, several studies have demonstrated the positive effects of platelets on the healing process of tendons. A local injection of ... [more ▼]

Introduction: The tendon is a tissue which does not heal easily. Recently, several studies have demonstrated the positive effects of platelets on the healing process of tendons. A local injection of platelet–rich plasma (PRP), which releases in situ many growth factors, has the potentiality to enhance the tendon healing process. The aim of our experiment was to ascertain by an original mechanical measure whether the use of PRP was of interest for accelerating the healing process of rats’ Achilles tendons after surgical induced lesion. Methods: A 5mm defect was surgically induced in 90 rats’ Achilles tendon. Rats were divided into 2 groups of 45: (A) control (no treatment) and (B) PRP treatment. Rats of group B received a PRP injection in situ after the surgery. Afterwards, rats of both groups were placed in their cages without immobilization. After 5, 15 and 30 days, 10 traumatized Achilles tendons of each group were dissected and removed. Immediately after sampling, tendons were submitted to a biomechanical tensile test up to rupture, using a “Cryo-jaw”. After that, transcriptomic analyses were made on the tendon samples, to study the expression of type III collagen, matrix metalloproteases and tenomodulin. A hydroxyproline dosage was done to quantify the collagen in the tendon during its healing process. Tendons of the 15 remaining rats of each group were subjected to a histological study, respectively at day 5, 15 and 30 (5 rats for each time). Results: We demonstrated that the force necessary to induce tendon rupture during biomechanical tensile test study was greater for tendons which had been submitted to an injection of PRP compared to the control group: +19% (day 5), +30% (day 15) and +43% (day 30). Histological study showed that PRP could enhance cells proliferation, angiogenesis and collagen organisation. Our biochemical analyses did not explain beneficial effects of PRP. Indeed, there was no significant difference neither between the expression of different studied genes, nor in the quantity of hydroxyproline between both groups. Conclusion: This experimentation has shown that a PRP injection could accelerate the tendons healing process and improve its quality. [less ▲]

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See detailRhoGDI alpha-dependent balance between RhoA and RhoC is a key regulator of cancer cell tumorigenesis
Ho, Thi Thanh Giang ULg; Stultiens, Audrey; Dubail, Johanne ULg et al

in Molecular Biology of the Cell (2011), 22(17), 3263-75

RhoGTPases are key signaling molecules regulating main cellular functions such as migration, proliferation, survival, and gene expression through interactions with various effectors. Within the RhoA ... [more ▼]

RhoGTPases are key signaling molecules regulating main cellular functions such as migration, proliferation, survival, and gene expression through interactions with various effectors. Within the RhoA-related subclass, RhoA and RhoC contribute to several steps of tumor growth, and the regulation of their expression affects cancer progression. Our aim is to investigate their respective contributions to the acquisition of an invasive phenotype by using models of reduced or forced expression. The silencing of RhoC, but not of RhoA, increased the expression of genes encoding tumor suppressors, such as nonsteroidal anti-inflammatory drug-activated gene 1 (NAG-1), and decreased migration and the anchorage-independent growth in vitro. In vivo, RhoC small interfering RNA (siRhoC) impaired tumor growth. Of interest, the simultaneous knockdown of RhoC and NAG-1 repressed most of the siRhoC-related effects, demonstrating the central role of NAG-1. In addition of being induced by RhoC silencing, NAG-1 was also largely up-regulated in cells overexpressing RhoA. The silencing of RhoGDP dissociation inhibitor alpha (RhoGDIalpha) and the overexpression of a RhoA mutant unable to bind RhoGDIalpha suggested that the effect of RhoC silencing is indirect and results from the up-regulation of the RhoA level through competition for RhoGDIalpha. This study demonstrates the dynamic balance inside the RhoGTPase network and illustrates its biological relevance in cancer progression. [less ▲]

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See detailDevelopment of a Chitosan Nanofibrillar Scaffold for Skin Repair and Regeneration.
Tchemtchoua Tateu, Victor ULg; Atanasova, G.; Aqil, Abdelhafid ULg et al

in Biomacromolecules (2011), 12

The final goal of the present study was the development of a 3-D chitosan dressing that would shorten the healing time of skin wounds by stimulating migration, invasion, and proliferation of the relevant ... [more ▼]

The final goal of the present study was the development of a 3-D chitosan dressing that would shorten the healing time of skin wounds by stimulating migration, invasion, and proliferation of the relevant cutaneous resident cells. Three-dimensional chitosan nanofibrillar scaffolds produced by electrospinning were compared with evaporated films and freeze-dried sponges for their biological properties. The nanofibrillar structure strongly improved cell adhesion and proliferation in vitro. When implanted in mice, the nanofibrillar scaffold was colonized by mesenchymal cells and blood vessels. Accumulation of collagen fibrils was also observed. In contrast, sponges induced a foreign body granuloma. When used as a dressing covering full-thickness skin wounds in mice, chitosan nanofibrils induced a faster regeneration of both the epidermis and dermis compartments. Altogether our data illustrate the critical importance of the nanofibrillar structure of chitosan devices for their full biocompatibility and demonstrate the significant beneficial effect of chitosan as a wound-healing biomaterial. [less ▲]

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