Pathological investigations on sperm whales stranded on Belgian and Dutch coasts

in Sperm whale deaths in the North Sea Science and management (1995)

Causes de mortalité et teneur en métaux lourds de Guillemots de Troïl Uria aalge échoués le long du littoral belge.

in Bulletin de la Société Royale des Sciences de Liège (1994), 63(1-2), 211-217

Oiled seabirds at the belgian coast

in annual proceeding (1993)

Tuberculosis in dogs : a case report and review of the literature

in Journal of the American Animal Hospital Association (1992), 28

Infestation de chiots par Neospora Canis en Belgique et en France

in Annales de Médecine Vétérinaire (1990), (134), 167-171

Experimental Infection of Bulls with a Genital Isolate of Bovine Herpesvirus-4 and Reactivation of Latent Virus with Dexamethasone

in Veterinary Microbiology (1989), 21(2), 97-114

Five 13- to 18-month old Belgian Blue bulls were used in this experiment. Four bulls (Nos. 2, 3, 4 and 5) were inoculated intratesticularly with 10(5) plaque-forming units of bovine herpesvirus-4 (BHV-4 ... [more ▼]

Five 13- to 18-month old Belgian Blue bulls were used in this experiment. Four bulls (Nos. 2, 3, 4 and 5) were inoculated intratesticularly with 10(5) plaque-forming units of bovine herpesvirus-4 (BHV-4) in each testicle (Day 0). The challenge BHV-4 strain was previously isolated from testicle cells of a bull exhibiting orchitis and azoospermia. The fifth bull (No. 1) was used as a control and received the same volume of uninfected cell culture supernatant. For 5 days, beginning on Day 51 post-infection, two bulls (Nos. 4 and 5) and the control bull (No. 1) received 0.1 mg kg-1 of dexamethasone. Unilateral castrations were then performed at regular intervals for viral examination. Treatment with dexamethasone reactivated latent BHV-4, but no clinical signs were observed in treated bulls until the end of the experiment (Day 93). Only Bull 3 showed conjunctivitis and temporary azoospermia. The virus was recovered from various samples showing that: (i) BHV-4 can be present in a latent state in the testicles and mononuclear blood cells; (ii) dexamethasone reactivates the virus; (iii) the virus is excreted by nasal and ocular routes. Each infected bull seroconverted and a booster antibody response appeared after dexamethasone treatment as shown by immunofluorescence. Neutralizing antibodies were detected in each bull by complement-dependent neutralization test with titres higher than those obtained by a classical neutralization test. No booster response of neutralizing antibodies was observed after dexamethasone treatment. The antigenically relevant envelope BHV-4 proteins were identified by Western blotting using sera samples from the animals. DNA restriction endonuclease profiles of viruses reisolated after primary infection and reactivation showed only small differences. [less ▲]

Effect of trenbolone acetate associated with 17ß-oestradiol on reproductive performances of young growing fattening bulls

in Journal of Animal Physiology & Animal Nutrition (1988), 59

Bulls were implanted with an ear pellet of trenbolone acetate and oestradiol in association. Some treated bulls produced a sperm without spermatozoa but there were no significant differences in ... [more ▼]

Bulls were implanted with an ear pellet of trenbolone acetate and oestradiol in association. Some treated bulls produced a sperm without spermatozoa but there were no significant differences in spermatozoa concentration and sperm quality between treated animals which produced a sperm with spermatozoa and control bulls. The scrotal circumference of the treated animals did not change over the experimental period while that of control bulls regularly increased. After a dynamic stimuation with luteinzing hormone releasing hormone, the luteinizing hormone concentrations did not change in the treated animals and the testosterone kinetic profiles were characterized by a low basal concentration and small peak heights. The microscopic evaluation of the testicles indicated a reduction in the percentage of seminiferous tubules with mature spermatozoa and a reduction in the number of Leydig cells in the islets. [less ▲]