References of "Clercx, Cécile"
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See detailWhole body barometric plethysmography measurements in dogs with brachycephalic airway obstruction syndrome : preliminary results
Bernaerts, Frederique ULg; Talavera, J.; Le Garrérès, A. et al

in 15th ESVIM Meeting - Glasgow - Ecosse - Septembre 2005 (2005, September)

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See detailHistochemical and immunohistochemical characterization of canine nasopharyngeal lymphoid tissue
Billen, Frédéric ULg; Peeters, Dominique ULg; Dehard, Sandrine ULg et al

in 15th ESVIM Meeting - Glasgow - Ecosse - Septembre 2005 (2005, September)

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See detailComparison of bronchoalveolar lavage cytospins ans smears in small animals
Dehard, Sandrine ULg; Bernaerts, Frederique ULg; Peeters, Dominique ULg et al

in 15th ESVIM Meeting - Glasgow - UK - 2005 (2005, September)

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See detailWhole body barometric plethysmography measurements in dogs with brachycephalic airway obstructive syndrome
Bernaerts, Frederique ULg; Talavera, J.; Le Garrérès, Alain et al

Poster (2005, September)

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See detailWhole-body barometric plethysmography in healthy dogs : influence of jet nebulizer and bias flow rate on the results of a bronchoprovocative test
Talavera, J.; Bernaerts, Frederique ULg; Schuller, S. et al

in ACVIM Meeting - Baltimore (USA) - juin 2005 (2005, June)

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See detailSurgical treatment of canine nasal aspergillosis by rhynotomy combined with enilconazole infusion: preliminary results
Claeys, Stéphanie ULg; Lefebvre, Jean-Benoît; Schuller, Simone et al

in Veterinary Surgery : The Official Journal of the American College of Veterinary Surgeons (2005, May), 34(3), 14

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See detailAn immunohistochemical study of canine nasal aspergillosis
Peeters, Dominique ULg; Day, M. J.; Clercx, Cécile ULg

in Journal of Comparative Pathology (2005), 132(4), 283-288

In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most ... [more ▼]

In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most consistent histopathological finding was a severe, predominantly lymphoplasmacytic, inflammatory infiltration of the lamina propria. Fungal hyphae were not observed to invade the mucosa but were found at the mucosal surface and within material collected from the nasal cavity. The main immunohistochemical findings were (1) a predominance of IgG(+) plasma cells over IgA(+) and IgM(+) plasma cells, (2) significant numbers of macrophages and dendritic cells expressing MHC class 11 molecules, (3) macrophages and neutrophils expressing L1 antigen and (4) a mixture of CD4(+) and CD8(+) T cells. These findings are consistent with a dominant Th1-regulated cell-mediated immune response. The nature of the inflammatory infiltrate and the lack of invasiveness of the mucosa by the fungus, together with the clinical course of the disease and the apparent immunocompetence of the affected dogs, suggest that canine nasal aspergillosis resembles the chronic erosive non-invasive fungal sinusitis described in human patients. © 2004 Elsevier Ltd. All rights reserved. [less ▲]

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See detailBronchodilators in bronchoscopy-induced airflow limitation in allergen-sensitized cats.
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, François et al

in Journal of Veterinary Internal Medicine (2005), 19(2), 161-167

This study investigated the effect of bronchoscopy and bronchoalveolar lavage (BAL) on respiratory function, determined by barometric whole-body plethysmography (BWBP), of healthy and allergen-sensitized ... [more ▼]

This study investigated the effect of bronchoscopy and bronchoalveolar lavage (BAL) on respiratory function, determined by barometric whole-body plethysmography (BWBP), of healthy and allergen-sensitized cats. Furthermore, the efficacy of inhaled bronchodilators in preventing changes in respiratory function was determined. For test 1, 18 healthy experimental cats were investigated on day 1 by BWBP. On day 2, the cats underwent BWBP after sedation (medetomidine), after anesthesia induction (propofol), and after bronchoscopy and BAL. Enhanced pause (Penh) was significantly increased after bronchoscopy and BAL (1.64 +/- 0.17 versus 1.23 +/- 0.07, P < .05). For test 2, 6 cats were sensitized to ovalbumin (OVA), 6 cats were sensitized to Ascaris suum (AS), and 6 cats served as controls. On day 0, OVA- and AS-sensitized cats underwent an inhaled allergen challenge, whereas controls were exposed to saline. On days 1 and 2, the same protocol as described for test 1 was repeated. Post-BAL Penh of the AS-sensitized cats was significantly higher than at test 1 (2.28 +/- 0.22 versus 1.69 +/- 0.33, P < .05) and was correlated with BAL fluid neutrophil count (r = 0.55, P < .05). During tests 3, 4, and 5, the same protocol as used for test 2 was applied to each cat group, with the animals being randomly treated before sedation with inhaled salbutamol (200 microg), ipratropium bromide (40 microg), or a combination of both (200 + 40 microg). Post-BAL Penh of the AS-sensitized group was significantly decreased after the salbutamol + ipratropium bromide treatment (1.56 +/- 0.18 versus 2.28 +/- 0.22, P < .05). This study suggests that bronchoscopy and BAL induce airflow limitation in cats, which is more severe in the presence of lower airway inflammation. Inhaled salbutamol + ipratropium bromide reduce BAL-induced bronchoconstriction in AS-challenged cats and might be recommended as preventive treatment of asthmatic cats undergoing bronchoscopy [less ▲]

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See detailCollection of exhaled breath condensate and analysis of hydrogen peroxide as a potential marker of lower airway inflammation in cats
Kirschvink, N.; Marlin, D.; Castermans, Françoise ULg et al

in Veterinary Journal (2005), 169(3), 385-396

The objective of this study was to describe a standardised and non-invasive method for exhaled breath condensate (EBC) collection in cats and to test whether determination of hydrogen peroxide (H2O2) in ... [more ▼]

The objective of this study was to describe a standardised and non-invasive method for exhaled breath condensate (EBC) collection in cats and to test whether determination of hydrogen peroxide (H2O2) in EBC might be used as marker of lower airway inflammation. The technique of barometric whole body plethysmography for cats was combined with a system to condense the effluent air from the plethysmograph, allowing simultaneous EBC collection and respiratory pattern measurement. H2O2 was determined spectrophotometrically. Eighteen experimental cats were used to investigate the impact on EBC volume and EBC H2O2 of plethysmograph ventilation rate, collection duration, sample stability, within-day and day-to-day variability. After determination of a standardised EBC collection procedure, correlation analyses between EBC H2O2 and bronchoalveolar lavage (BAL) cytology of healthy and allergen-challenged Ascaris suum (AS)-sensitised cats were performed. A significant and positive correlation between EBC H2O2 and bronchoalveolar lavage (BAL) neutrophil% was found in healthy cats (P < 0.001, r = 0.55), whereas in AS-sensitised cats, correlation with BAL eosinophil% was significant (P < 0.005, r = 0.61). H2O2 was increased after an allergen challenge in AS-sensitised cats (n = 6, 0.56 ± 0.12 versus 1.08 ± 0.35 μ mol/L, P < 0.05). This study proposes a non-invasive, well tolerated and repeatable method of EBC collection for cats and suggests that EBC H2O2 might be used as non-invasive biomarker for monitoring lower airway inflammation. [less ▲]

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See detailQuantification of mRNA encoding cytokines, chemokines and CCR3 in bronchial biopsies from dogs with eosinophilic bronchopneumopathy.
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Proceedings of the 15th Annual Congress of the ECVIM-CA (2005)

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See detailQuantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis.
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Proceedings of the 15th Annual Congress of the ECVIM-CA (2005)

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See detailInhaled and systemic corticosteroids decrease Matrix Metalloproteinase-9 in bronchoalveolar lavage fluid of asthmatic cats
Leemans, Jérôme ULg; Kirschvink, Nathalie; Delvaux, François ULg et al

in In Proceedings:15th Congress European College of Veterinary Internal Medicine – companion animals (2005)

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See detailEffect of oral and inhaled corticosteroids on bronchial reactivity and airway inflammation in a feline model of asthma
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, François ULg et al

in In proceedings: 15th Congress European College of Veterinary Internal Medicine – companion animals (2005)

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See detailPrednisolone reduces 8-Iso-PGF2alpha in a feline model of asthma
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, F. et al

in European Respiratory Journal (2005), 137s

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See detailReal-time RT-PCR quantification of mRNA encoding cytokines and chemokines in histologically normal canine nasal, bronchial and pulmonary tissue
Peeters, Dominique ULg; Peters, I. R.; Farnir, Frédéric ULg et al

in Veterinary Immunology and Immunopathology (2005), 104(3-4), 195-204

Cytokines and chemokines are likely to be involved in the pathogenesis of inflammatory diseases of the canine respiratory tract. The roles and relative amounts of these molecules have not yet been defined ... [more ▼]

Cytokines and chemokines are likely to be involved in the pathogenesis of inflammatory diseases of the canine respiratory tract. The roles and relative amounts of these molecules have not yet been defined in the respiratory mucosa of normal dogs or dogs with naturally acquired respiratory inflammation. In the present study, real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays were employed to quantify messenger RNA (mRNA) encoding the chemokines monocyte chernotactic protein (MCP)-2, eotaxin-2 and eotaxin-3, and the cytokines interleukin-4 (IL-4), IL-5, IL-6, IL-10, IL-12p40, IL-18, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) in normal nasal, bronchial and pulmonary tissues from puppies (n = 4) and from adult dogs (n = 7). There was no significant difference in the expression of any transcript between puppies and adult dogs at any of the anatomical sites examined. The expression of mRNA encoding eotaxin-2 and eotaxin-3 increased significantly with progression from the nasal mucosa to pulmonary parenchyma but expression of MCP-2 mRNA did not show this trend. At all levels of the respiratory mucosa, the most abundant transcripts were those encoding IL-18 and TGF-beta. Transcripts encoding IL-6, IL-10, IL-12 and TNF-alpha were approximately ten-fold less abundant, and IL-4, IL-5 and IFN-gamma were the least abundant templates. There was significantly different amount of mRNA encoding IL-5, IL- 18 and TNF-alpha between particular anatomical levels of the respiratory mucosa while the mRNA expression of the other cytokines was similar at all anatomical sites. The results of the present study will enable comparisons to be made with results obtained from similar samples obtained from dogs with nasal, bronchial or pulmonary diseases. (c) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailDistribution of leucocyte subsets in the canine respiratory tract
Peeters, Dominique ULg; Day, M. J.; Farnir, Frédéric ULg et al

in Journal of Comparative Pathology (2005), 132(4), 261-272

Histochemistry and immunohistochemistry were used to characterize leucocyte subsets in the respiratory tract of 15 outbred dogs (five aged <6 months and 10 aged >1 year) that had no evidence of ... [more ▼]

Histochemistry and immunohistochemistry were used to characterize leucocyte subsets in the respiratory tract of 15 outbred dogs (five aged <6 months and 10 aged >1 year) that had no evidence of respiratory disease. No organized nose- or bronchus-associated lymphoid tissue was observed in any of the sections examined. IgA(+) plasma cells predominated in nasal mucosa and in all parts of the bronchial tree, with fewer IgG(+) and IgM(+) plasma cells. The numbers of IgA(+) and IgM(+) cells were significantly greater in the nasal mucosa than in any other part of the respiratory mucosa. There were significantly fewer IgA(+), IgG(+) and IgM(+) cells in all parts of the respiratory tract in the puppies than in the adults. The number and distribution of mast cells and cells expressing MHC class II, L1 or CD1c were recorded. Mast cells were mainly found in the subepithelial lamina propria of nasal and bronchial mucosa and in the alveolar interstitium, and cells expressing IgE had a similar distribution. Mast cells were also present within muscle layers of the bronchial tree. The numbers of mast cells and MHC class II(+) cells were significantly greater in the nasal mucosa than in any other part of the respiratory mucosa. In the nose, carina and primary and secondary bronchus, there were significantly more mast cells and MHC class II(+) cells in puppies than in adult dogs, whereas the numbers of L1(+) cells and CD1c(+) cells in most sites were significantly greater in older dogs. There were significantly more CD3(+) and CD8(+) cells in the nasal mucosa than in any part of the bronchial mucosa. In most parts of the respiratory mucosa, CD4(+), CD8(+) and TCR alphabeta(+) cells were present in significantly greater numbers in adults than in puppies. All parts of the respiratory tract had similar numbers of mucosal CD4(+) and CD8(+) T lymphocytes. TCR gammadelta(+) cells were absent or sparse in all samples. These data, obtained from dogs without respiratory disease, will enable comparisons to be made with dogs suffering from infectious or inflammatory nasal, bronchial and pulmonary diseases. [less ▲]

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See detailDistribution of leucocyte subsets in bronchial mucosa from dogs with eosinophilic bronchopneumopathy
Peeters, Dominique ULg; Day, M. J.; Clercx, Cécile ULg

in Journal of Comparative Pathology (2005), 133(2-3, Aug-Oct), 128-135

Immunohistochemistry was used to characterize the distribution of leucocyte subsets in the bronchial mucosa of 11 dogs with idiopathic eosinophilic bronchopneumopathy (EBP). Formalin-fixed tissues from ... [more ▼]

Immunohistochemistry was used to characterize the distribution of leucocyte subsets in the bronchial mucosa of 11 dogs with idiopathic eosinophilic bronchopneumopathy (EBP). Formalin-fixed tissues from all dogs were included in the study, but frozen tissue from only one dog was available. MHC class II(+) cells were found in moderate numbers in the lamina propria (LP). These cells were morphologically either dendritic-like cells or macrophages, but many macrophages did not express MHC class II. Such molecules were expressed by occasional fibroblasts. L1(+) cells, which formed a relatively small component of the LP inflammatory infiltrate, were morphologically either macrophages or polymorphonuclear cells (probably eosinophils). IgA(+) plasma cells were found in varying numbers in the LP, mostly in association with glandular tissue. IgG(+) plasma cells were less common, and IgM(+) plasma cells were present in low numbers. Many CD3(+) cells were present in the LP. In the single case from which frozen tissue was available, most of the lymphocytes were labelled with CD4 marker, while smaller numbers were CD8(+) T cells. Most of the lymphocytes in this case were positively labelled with T-cell receptor (TCR)-alphabeta marker. TCR-gammadelta(+) cells, although less common, were present in significant numbers throughout the LP. CDlc(+) dendritic cells were numerous in the epithelium and in the LP, immediately beneath the basement membrane. These findings, which were similar to those described in human asthma, are suggestive of a Th2 dominant immune response in canine EBP. As in human asthma, this provides a possible basis for new forms of treatment in canine EBP. [less ▲]

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See detailEvaluation of the bronchoprotective effects of inhaled salmeterol, fenoterol and oxitropium in healthy cats
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, F. et al

in Fundamental & Clinical Pharmacology (2005), 19

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See detailMMP-9 activity and 8-ISO-PGF2alphaare increased in bronchoalveolar lavage fluid in a model of feline asthma
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, F. et al

in Proceedings of the American College of Veterinary Internal Medicine, Baltimore, Maryland (2005)

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See detailRepeatability of allergen challenges in a feline model of asthma : effect on airway reactivity and airway inflammation
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, F. et al

in Proceedings of the American Thoracic Society, San Diego, California (2005)

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