References of "Clercx, Cécile"
     in
Bookmark and Share    
Peer Reviewed
See detailCytokine and chemokine expression in canine sino-nasal aspergillosis and idiopathic lymphoplasmacytic rhinosinusitis.
Peeters, Dominique ULg; Peters, I. R.; Helps, C. et al

in Proceedings of the 16th Annual Congress of the ECVIM-CA (2006)

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailDistribution of leucocyte subsets in the canine pharyngeal tonsil
Billen, Frédéric ULg; Peeters, Dominique ULg; Dehard, Sandrine ULg et al

in Journal of Comparative Pathology (2006), 135(2-3, Aug-Oct), 63-73

This report describes the distribution and nature of lymphoid tissue in the nasopharyngeal mucosa of six puppies (mean age +/- SD, 0.3 +/- 0.25 years) and eight adult dogs (mean age +/- SD, 8.8 +/- 2.67 ... [more ▼]

This report describes the distribution and nature of lymphoid tissue in the nasopharyngeal mucosa of six puppies (mean age +/- SD, 0.3 +/- 0.25 years) and eight adult dogs (mean age +/- SD, 8.8 +/- 2.67 years) without respiratory disease. A non-encapsulated area of organized mucosa-associated lymphoid tissue was observed in the caudal part of the posterior wall of the nasopharynx, distal to the openings of the auditory tubes. This structure was consistent with the pharyngeal tonsil and was microscopically more extensive in puppies than in adult dogs. Histochemistry and immunohistochemistry were used to characterize and enumerate the leucocyte subsets in this part of the nasopharynx. Mast cells were found immediately beneath the respiratory epithelium but were also scattered in the glandular and muscular tissue. IgA(+) plasma cells outnumbered IgG(+) and IgM(+) plasma cells, especially in the glandular tissue. All classes of plasma cells were present in significantly greater numbers in adults than in puppies. MHC class II+ cells were mainly observed in areas containing diffuse and follicular aggregates of lymphoid cells. Both MHC class II+ cells and CD1c(+) cells with a dendritic morphology were predominantly found immediately beneath or within the epithelium, and cells expressing these markers were more abundant in puppies than in adult dogs. The anti-L1 marker labelled low numbers of cells with a neutrophilic morphology, which were significantly more abundant in puppies than in adult dogs. The majority of lymphoid cells were CD3(+) T lymphocytes and these were particularly abundant in areas containing aggregates of lymphold cells; CD4(+), CD8(+) and TCR alpha beta(+) cells had the same distribution as the CD3(+) cells. CD4(+) cells were more numerous than CD8(+) cells. The quantitative and qualitative data obtained will enable comparisons to be made with similar studies in dogs suffering from nasopharyngeal diseases, or when the local immune system needs to be investigated. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 30 (3 ULg)
Full Text
Peer Reviewed
See detailQuantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Veterinary Microbiology (2006), 114(3-4), 318-326

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We ... [more ▼]

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We investigated the nature of the local immune response mounted in canine sino-nasal aspergillosis. Quantitative RT-PCR was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify mRNA encoding a panel of cytokines and chemokines. Canine sino-nasal aspergillosis was associated with significantly increased expression of mRNA encoding MCP-1, -2, -3 and -4, IL-8, IL-10, EL-18 and TNF-alpha relative to controls (P < 0.01) but there was no difference between groups with respect to IL-4, IL-5, IL-6, IL-12, TGF-beta, and eotaxin-2 and -3. The up-regulation of proinflammatory cytokines and chemokines related to the influx of phagocytic cells might account for the localisation of this infection to the upper respiratory tract. The up-regulation of the expression of the immunomodulatory cytokine IL-10 in nasal tissue from affected dogs might be important in limiting the extent of local tissue destruction, but might also account for the fact that infected dogs are generally unable to clear this infection spontaneously. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 20 (0 ULg)
Full Text
Peer Reviewed
See detailReal-time RT-PCR quantification of mRNA encoding cytokines, CC chemokines and CCR3 in bronchial biopsies from dogs with eosinophilic bronchopneurnopathy
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Veterinary Immunology and Immunopathology (2006), 110(1-2), 65-77

Idiopathic canine eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the pulmonary interstitium and bronchial mucosa, a cause for which has not yet been ... [more ▼]

Idiopathic canine eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the pulmonary interstitium and bronchial mucosa, a cause for which has not yet been discovered. A recent study, examining the relative proportion of various lymphocyte cell subsets within bronchoalveolar lavage fluid from dogs with EBP, has shown a selective increase in CD4(+) T-cells and a selective decrease in CD8(+) T-cells, suggesting that a similar Th2 immune response might occur in EBP. The aim of the present study was to determine the profile of cytokine, chemokine and CC chemokine receptor 3 (CCR3) messenger RNA (mRNA) expression in bronchial tissue from dogs with EBP. Real-time RT-PCR assays were used for the quantification of mRNA encoding for a panel of cytokines, CC chemokines and CCR3 in perendoscopic bronchial biopsies from eight dogs with EBP and seven age-matched control dogs. Messenger RNA transcribed from the housekeeping gene glyceraldehyde-3 -phosphate dehydrogenase was used for normalisation of the threshold cycle in order to determine the relative copy numbers of the transcripts. No significant difference in the expression of any cytokine, MCP-1, -2, -4 and CCR3 was found between control and EBP dogs. The expression of transcript for MCP-3, eotaxin-2 and -3 was significantly greater in bronchial biopsies from dogs with EBP than in samples from control dogs while there was significantly less mRNA encoding RANTES in the mucosa of dogs with EBP. In conclusion, the cytokine mRNA expression profile in perendoscopic bronchial biopsies is similar in dogs with EBP and dogs without respiratory disease. Further studies on the quantification of mRNA encoding cytokines in isolated T lymphocytes from bronchoalveolar lavage fluid or bronchial biopsies are needed before any conclusion on the cytokine profile in canine EBP can be drawn. Eotaxin-2, -3 and MCP-3 appear to be implicated in the pathogenesis of the disease. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 35 (3 ULg)
Peer Reviewed
See detailOmega 3 fatty acid complementation (fish oil) in an experimental model of feline asthma : effect on bronchial reactivity, airway inflammation and oxidative stress markers
Kirschvinck, N.; Leemans, Jérôme ULg; Delvaux, F. et al

in Proceedings: 24th Symposium of the Veterinary Comparative Respiratory Society,”Respiratory infections and zoonoses (2006)

Detailed reference viewed: 15 (1 ULg)
Peer Reviewed
See detailDiagnostic impact of thoracic radiography in an experimental model of feline asthma
Kirschvinck, N.; Kersnak, E.; Leemans, Jérôme ULg et al

in Proceedings: 24th Symposium of the Veterinary Comparative Respiratory Society,”Respiratory infections and zoonoses" (2006)

Detailed reference viewed: 16 (0 ULg)
Peer Reviewed
See detailEffect of omega-3 fatty acid supplementation (fish oil) on bronchial reactivity, airway inflammation and oxidative stress markers in a feline model of asthma
Kirschvinck, N.; Leemans, Jérôme ULg; Delvaux, F. et al

in Proceedings: 16th Congress European College of Veterinary Internal Medicine – companion animals (2006)

Detailed reference viewed: 20 (1 ULg)
Peer Reviewed
See detailBronchoprotective effect of inhaled salmeterol, salbutamol and ipratropium bromide using different devices on muscarinic bronchoconstriction in healthy cats
Leemans, Jérôme ULg; Kirschvinck, N.; Bernaerts, F. et al

in Proceedings: 16th Congress European College of Veterinary Internal Medicine – companion animals (2006)

Detailed reference viewed: 53 (2 ULg)
Full Text
Peer Reviewed
See detailSuspected drug-induced destructive cholangitis in a young dog.
Gabriel, Alexandra; van den Ingh, Ted; Clercx, Cécile ULg et al

in Journal of Small Animal Practice (2006), 47(6)

Detailed reference viewed: 34 (0 ULg)
Full Text
Peer Reviewed
See detailLaryngeal paralysis-polyneuropathy complex in young related Pyrenean Mountain dogs.
Gabriel, Alexandra; Poncelet, Luc; Van Ham, Luc et al

in Journal of Small Animal Practice (2006), 47(3)

Detailed reference viewed: 14 (1 ULg)
Peer Reviewed
See detailProcollagen type-III aminoterminal peptide in serum and bronchoalveolar lavage fluid in dogs with chronic bronchopneumopathies – preliminary results
Schuller, S.; Talavera, J.; Jespers, P. et al

in 15th ESVIM Meeting - Glasgow - Ecosse - Septembre 2005 (2005, September)

Detailed reference viewed: 7 (0 ULg)
Peer Reviewed
See detailWhole body barometric plethysmography measurements in dogs with brachycephalic airway obstruction syndrome : preliminary results
Bernaerts, Frederique ULg; Talavera, J.; Le Garrérès, A. et al

in 15th ESVIM Meeting - Glasgow - Ecosse - Septembre 2005 (2005, September)

Detailed reference viewed: 13 (1 ULg)
Peer Reviewed
See detailHistochemical and immunohistochemical characterization of canine nasopharyngeal lymphoid tissue
Billen, Frédéric ULg; Peeters, Dominique ULg; Dehard, Sandrine ULg et al

in 15th ESVIM Meeting - Glasgow - Ecosse - Septembre 2005 (2005, September)

Detailed reference viewed: 2 (0 ULg)
Peer Reviewed
See detailComparison of bronchoalveolar lavage cytospins ans smears in small animals
Dehard, Sandrine ULg; Bernaerts, Frederique ULg; Peeters, Dominique ULg et al

in 15th ESVIM Meeting - Glasgow - UK - 2005 (2005, September)

Detailed reference viewed: 20 (3 ULg)
Peer Reviewed
See detailWhole body barometric plethysmography measurements in dogs with brachycephalic airway obstructive syndrome
Bernaerts, Frederique ULg; Talavera, J.; Le Garrérès, Alain et al

Poster (2005, September)

Detailed reference viewed: 29 (4 ULg)
Peer Reviewed
See detailWhole-body barometric plethysmography in healthy dogs : influence of jet nebulizer and bias flow rate on the results of a bronchoprovocative test
Talavera, J.; Bernaerts, Frederique ULg; Schuller, S. et al

in ACVIM Meeting - Baltimore (USA) - juin 2005 (2005, June)

Detailed reference viewed: 17 (1 ULg)
Full Text
Peer Reviewed
See detailSurgical treatment of canine nasal aspergillosis by rhynotomy combined with enilconazole infusion: preliminary results
Claeys, Stéphanie ULg; Lefebvre, Jean-Benoît; Schuller, Simone et al

in Veterinary Surgery : The Official Journal of the American College of Veterinary Surgeons (2005, May), 34(3), 14

Detailed reference viewed: 46 (10 ULg)
Full Text
Peer Reviewed
See detailAn immunohistochemical study of canine nasal aspergillosis
Peeters, Dominique ULg; Day, M. J.; Clercx, Cécile ULg

in Journal of Comparative Pathology (2005), 132(4), 283-288

In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most ... [more ▼]

In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most consistent histopathological finding was a severe, predominantly lymphoplasmacytic, inflammatory infiltration of the lamina propria. Fungal hyphae were not observed to invade the mucosa but were found at the mucosal surface and within material collected from the nasal cavity. The main immunohistochemical findings were (1) a predominance of IgG(+) plasma cells over IgA(+) and IgM(+) plasma cells, (2) significant numbers of macrophages and dendritic cells expressing MHC class 11 molecules, (3) macrophages and neutrophils expressing L1 antigen and (4) a mixture of CD4(+) and CD8(+) T cells. These findings are consistent with a dominant Th1-regulated cell-mediated immune response. The nature of the inflammatory infiltrate and the lack of invasiveness of the mucosa by the fungus, together with the clinical course of the disease and the apparent immunocompetence of the affected dogs, suggest that canine nasal aspergillosis resembles the chronic erosive non-invasive fungal sinusitis described in human patients. © 2004 Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 18 (1 ULg)
Full Text
Peer Reviewed
See detailBronchodilators in bronchoscopy-induced airflow limitation in allergen-sensitized cats.
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, François et al

in Journal of Veterinary Internal Medicine (2005), 19(2), 161-167

This study investigated the effect of bronchoscopy and bronchoalveolar lavage (BAL) on respiratory function, determined by barometric whole-body plethysmography (BWBP), of healthy and allergen-sensitized ... [more ▼]

This study investigated the effect of bronchoscopy and bronchoalveolar lavage (BAL) on respiratory function, determined by barometric whole-body plethysmography (BWBP), of healthy and allergen-sensitized cats. Furthermore, the efficacy of inhaled bronchodilators in preventing changes in respiratory function was determined. For test 1, 18 healthy experimental cats were investigated on day 1 by BWBP. On day 2, the cats underwent BWBP after sedation (medetomidine), after anesthesia induction (propofol), and after bronchoscopy and BAL. Enhanced pause (Penh) was significantly increased after bronchoscopy and BAL (1.64 +/- 0.17 versus 1.23 +/- 0.07, P < .05). For test 2, 6 cats were sensitized to ovalbumin (OVA), 6 cats were sensitized to Ascaris suum (AS), and 6 cats served as controls. On day 0, OVA- and AS-sensitized cats underwent an inhaled allergen challenge, whereas controls were exposed to saline. On days 1 and 2, the same protocol as described for test 1 was repeated. Post-BAL Penh of the AS-sensitized cats was significantly higher than at test 1 (2.28 +/- 0.22 versus 1.69 +/- 0.33, P < .05) and was correlated with BAL fluid neutrophil count (r = 0.55, P < .05). During tests 3, 4, and 5, the same protocol as used for test 2 was applied to each cat group, with the animals being randomly treated before sedation with inhaled salbutamol (200 microg), ipratropium bromide (40 microg), or a combination of both (200 + 40 microg). Post-BAL Penh of the AS-sensitized group was significantly decreased after the salbutamol + ipratropium bromide treatment (1.56 +/- 0.18 versus 2.28 +/- 0.22, P < .05). This study suggests that bronchoscopy and BAL induce airflow limitation in cats, which is more severe in the presence of lower airway inflammation. Inhaled salbutamol + ipratropium bromide reduce BAL-induced bronchoconstriction in AS-challenged cats and might be recommended as preventive treatment of asthmatic cats undergoing bronchoscopy [less ▲]

Detailed reference viewed: 27 (3 ULg)
Full Text
Peer Reviewed
See detailCollection of exhaled breath condensate and analysis of hydrogen peroxide as a potential marker of lower airway inflammation in cats
Kirschvink, N.; Marlin, D.; Castermans, Françoise ULg et al

in Veterinary Journal (2005), 169(3), 385-396

The objective of this study was to describe a standardised and non-invasive method for exhaled breath condensate (EBC) collection in cats and to test whether determination of hydrogen peroxide (H2O2) in ... [more ▼]

The objective of this study was to describe a standardised and non-invasive method for exhaled breath condensate (EBC) collection in cats and to test whether determination of hydrogen peroxide (H2O2) in EBC might be used as marker of lower airway inflammation. The technique of barometric whole body plethysmography for cats was combined with a system to condense the effluent air from the plethysmograph, allowing simultaneous EBC collection and respiratory pattern measurement. H2O2 was determined spectrophotometrically. Eighteen experimental cats were used to investigate the impact on EBC volume and EBC H2O2 of plethysmograph ventilation rate, collection duration, sample stability, within-day and day-to-day variability. After determination of a standardised EBC collection procedure, correlation analyses between EBC H2O2 and bronchoalveolar lavage (BAL) cytology of healthy and allergen-challenged Ascaris suum (AS)-sensitised cats were performed. A significant and positive correlation between EBC H2O2 and bronchoalveolar lavage (BAL) neutrophil% was found in healthy cats (P < 0.001, r = 0.55), whereas in AS-sensitised cats, correlation with BAL eosinophil% was significant (P < 0.005, r = 0.61). H2O2 was increased after an allergen challenge in AS-sensitised cats (n = 6, 0.56 ± 0.12 versus 1.08 ± 0.35 μ mol/L, P < 0.05). This study proposes a non-invasive, well tolerated and repeatable method of EBC collection for cats and suggests that EBC H2O2 might be used as non-invasive biomarker for monitoring lower airway inflammation. [less ▲]

Detailed reference viewed: 24 (3 ULg)