References of "Clercx, Cécile"
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See detailDiagnosis of pharyngeal disorders in dogs: a retrospective study of 67 cases
Billen, Frédéric ULg; Day, M. J.; Clercx, Cécile ULg

in Journal of Small Animal Practice (2006), 47(3), 122-129

OBJECTIVES: To assess the frequency distribution of pharyngeal disorders and to compare clinical signs in diseases of the different pharyngeal areas. To review the investigation methods. METHODS: Medical ... [more ▼]

OBJECTIVES: To assess the frequency distribution of pharyngeal disorders and to compare clinical signs in diseases of the different pharyngeal areas. To review the investigation methods. METHODS: Medical records of 67 dogs presenting with abnormalities of the pharyngeal area were retrospectively reviewed and classified according to the anatomic area involved, i.e. the nasopharyngeal, oropharyngeal and laryngopharyngeal areas. Clinical signs, endoscopic findings and results of additional sampling were reviewed. RESULTS: Nasopharyngeal disorders were most frequently encountered (49 per cent), with choanal masses being the most frequent diagnosis (24 of 33), followed by laryngopharyngeal disorders (37.5 per cent) and oropharyngeal disorders (10.5 per cent). A rare condition, stenosis of the intrapharyngeal opening, was classified separately (3 per cent). An overall good correlation between matched cytology and histopathology samples was found. In all categories of diseases, clinical signs related to both the upper respiratory and digestive tracts were reported. CLINICAL SIGNIFICANCE: Pharyngeal disorders are more frequently localised in the nasopharyngeal area and include essentially choanal masses. The use of a flexible endoscope for retrograde rhinoscopy is essential for adequate investigation of the proximal nasopharyngeal area. Clinical signs do not allow differentiation of the pharyngeal disorder within the different pharyngeal areas. [less ▲]

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See detailRespiratory diseases
Clercx, Cécile ULg

in Suter, P. F.; Kohn, B. (Eds.) Praktikum der Hundklinik, 10th Edition (2006)

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See detailInhaled fluticasone reduces bronchial responsiveness and airway inflammation in cats with mild chronic bronchitis
Kirschvink, N; Leemans, Jérôme ULg; Delvaux, François et al

in Journal of Feline Medicine and Surgery (2006), 8(1), 45-54

This study investigated the effect of inhaled fluticasone on lower airway inflammation and bronchial responsiveness (BR) to inhaled carbachol in cats with very mild, chronic bronchitis (n = 5) that were ... [more ▼]

This study investigated the effect of inhaled fluticasone on lower airway inflammation and bronchial responsiveness (BR) to inhaled carbachol in cats with very mild, chronic bronchitis (n = 5) that were compared with healthy cats serving as controls (n = 6). Chest radiographs, BR tests performed non-invasively by barometric whole body plethysmography (BWBP) and bronchoalveolar lavage (BAL) were performed before and after treatment. BR was quantified by calculating the concentration of carbachol inducing bronchoconstriction (C-Penh300%), defined as a 300% increase of baseline Penh, an index of bronchoconstriction obtained by BWBP. BAL fluid was analyzed cytologically and the oxidant marker 8-iso-PGF2α was determined. At test 1, healthy cats and cats with bronchitis were untreated, whereas for test 2 inhalant fluticasone (250 μg once daily) was administrated for 2 consecutive weeks to cats with bronchitis. Control cats remained untreated. Inhaled fluticasone induced a significant increase in C-Penh300% and a significant decrease of BAL fluid total cells, macrophages, neutrophils and 8-iso-PGF2α in cats with bronchitis, whilst untreated control cats did not show significant changes over time. This study shows that a 2-week fluticasone treatment significantly reduced lower airway inflammation in very mild bronchitis. BR could be successfully monitored in cats using BWPB and decreased significantly in response to inhaled fluticasone. 8-Iso-PGF2α in BAL fluid was responsive to treatment and appeared as a sensitive biomarker of lower airway inflammation in cats. [less ▲]

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See detailEffect of sampling method and culture conditions on the result of fungal culture in the diagnosis of canine sino-nasal aspergillosis.
Legarrérès, A.; Clercx, Cécile ULg; Mignon, Bernard ULg et al

in Proceedings of the 24th VCRS meeting (2006)

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See detailEvaluation of respiratory function by barometric whole-body plethysmography in healthy dogs
Talavera, J.; Kirschvink, N.; Schuller, S. et al

in Veterinary Journal (2006), 172(1), 67-77

The objective of the present study was to assess the validity of barometric whole-body plethysmography (BWBP), to establish reference values, and to standardise a bronchoprovocative test to investigate ... [more ▼]

The objective of the present study was to assess the validity of barometric whole-body plethysmography (BWBP), to establish reference values, and to standardise a bronchoprovocative test to investigate airway responsiveness using BWBP in healthy dogs. BWBP measurements were obtained from six healthy beagle dogs using different protocols: (1) during three consecutive periods (3.5 min each) in two morning and two evening sessions; (2) before and after administration of two protocols of sedation; (3) before and after nebulisation of saline and increasing concentrations of carbachol and histamine both in conscious dogs and in dogs under both protocols of sedation. Enhanced pause (PENH) was used as index of bronchoconstriction. Basal BWBP measurements were also obtained in 22 healthy dogs of different breeds, age and weight. No significant influence of either time spent in the chamber or daytime was found for most respiratory variables but a significant dog effect was detected for most variables. A significant body weight effect was found on tidal volume and peak flow values (P < 0.05). Response to carbachol was not reproducible and always associated with side effects. Nebulisation of histamine induced a significant increase in respiratory rate, peak expiratory flow, peak expiratory flow/peak inspiratory flow ratio and PENH (P < 0.05). The response was reproduced in each dog at different concentrations of histamine. Sedation with acepromazine + buprenorphine had little influence on basal measurements and did not change the results of histamine challenge. It was concluded that BWBP is a safe, non invasive and reliable technique of investigation of lung function in dogs which provides new opportunities to characterise respiratory status, to evaluate airway hyperresponsiveness and to assess therapeutic interventions. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailNon-invasive assessment of growth, gender and time of day related changes of respiratory pattern in healthy cats by use of barometric whole body plethysmography.
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, François ULg et al

in Veterinary Journal (2006), 172(3), 446-454

The objective of this study was to establish a reference base for respiratory variables (respiratory rate [R(R)], inspiratory and expiratory time [T(i) and T(e)], peak inspiratory and expiratory ... [more ▼]

The objective of this study was to establish a reference base for respiratory variables (respiratory rate [R(R)], inspiratory and expiratory time [T(i) and T(e)], peak inspiratory and expiratory pseudoflow [PIF and PEF], tidal volume [V(T)], minute ventilation [V(E)] and enhanced pause [Penh]) of healthy cats by use of barometric whole body plethysmography (BWBP). Eighteen healthy European cats (10 male, 8 female) were studied from the age of 3 to 13 months in order to assess growth- and gender-related changes of BWBP variables. Chest radiographs and bronchoalveolar lavage cytology were performed to confirm pulmonary health status. Diurnal changes were investigated every 2 h over a period of 24 h when the cats were adult. V(T), V(E), PIF and PEF significantly increased during somatic growth and were higher in males than in females, whereas R(R), T(i), T(e), T(e)/T(i) ratio, PEF/PIF ratio and Penh remained unchanged and were not affected by gender. When measured over 24 h, Penh, T(e) and T(i) were significantly increased in the early morning hours (04:00 h), whereas R(R), PIF and PEF were decreased at that time. This study provides reference values of BWBP variables for healthy male and female cats and indicates when circadian changes might be observed [less ▲]

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See detailImmune-mediated respiratory and cardiac disease
Clercx, Cécile ULg; Day, M. J.

in Clinical Immunology of the Dog and Cat (2006)

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See detailImmune-mediated respiratory and cardiac disease
Clercx, Cécile ULg

in Day, M. J. (Ed.) Clinical Immunology of the Dog and Cat”, 1st edition (2006)

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See detailDiagnosis of canine sino-nasal aspergillosis: is quantification of Aspergillus DNA a useful technique?
Peeters, Dominique ULg; Peters, I. R.; Helps et al

in Proceedings of the 24th VCRS meeting (2006)

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See detailCytokine and chemokine expression in canine sino-nasal aspergillosis and idiopathic lymphoplasmacytic rhinosinusitis.
Peeters, Dominique ULg; Peters, I. R.; Helps, C. et al

in Proceedings of the 16th Annual Congress of the ECVIM-CA (2006)

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See detailDistribution of leucocyte subsets in the canine pharyngeal tonsil
Billen, Frédéric ULg; Peeters, Dominique ULg; Dehard, Sandrine ULg et al

in Journal of Comparative Pathology (2006), 135(2-3, Aug-Oct), 63-73

This report describes the distribution and nature of lymphoid tissue in the nasopharyngeal mucosa of six puppies (mean age +/- SD, 0.3 +/- 0.25 years) and eight adult dogs (mean age +/- SD, 8.8 +/- 2.67 ... [more ▼]

This report describes the distribution and nature of lymphoid tissue in the nasopharyngeal mucosa of six puppies (mean age +/- SD, 0.3 +/- 0.25 years) and eight adult dogs (mean age +/- SD, 8.8 +/- 2.67 years) without respiratory disease. A non-encapsulated area of organized mucosa-associated lymphoid tissue was observed in the caudal part of the posterior wall of the nasopharynx, distal to the openings of the auditory tubes. This structure was consistent with the pharyngeal tonsil and was microscopically more extensive in puppies than in adult dogs. Histochemistry and immunohistochemistry were used to characterize and enumerate the leucocyte subsets in this part of the nasopharynx. Mast cells were found immediately beneath the respiratory epithelium but were also scattered in the glandular and muscular tissue. IgA(+) plasma cells outnumbered IgG(+) and IgM(+) plasma cells, especially in the glandular tissue. All classes of plasma cells were present in significantly greater numbers in adults than in puppies. MHC class II+ cells were mainly observed in areas containing diffuse and follicular aggregates of lymphoid cells. Both MHC class II+ cells and CD1c(+) cells with a dendritic morphology were predominantly found immediately beneath or within the epithelium, and cells expressing these markers were more abundant in puppies than in adult dogs. The anti-L1 marker labelled low numbers of cells with a neutrophilic morphology, which were significantly more abundant in puppies than in adult dogs. The majority of lymphoid cells were CD3(+) T lymphocytes and these were particularly abundant in areas containing aggregates of lymphold cells; CD4(+), CD8(+) and TCR alpha beta(+) cells had the same distribution as the CD3(+) cells. CD4(+) cells were more numerous than CD8(+) cells. The quantitative and qualitative data obtained will enable comparisons to be made with similar studies in dogs suffering from nasopharyngeal diseases, or when the local immune system needs to be investigated. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailQuantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Veterinary Microbiology (2006), 114(3-4), 318-326

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We ... [more ▼]

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We investigated the nature of the local immune response mounted in canine sino-nasal aspergillosis. Quantitative RT-PCR was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify mRNA encoding a panel of cytokines and chemokines. Canine sino-nasal aspergillosis was associated with significantly increased expression of mRNA encoding MCP-1, -2, -3 and -4, IL-8, IL-10, EL-18 and TNF-alpha relative to controls (P < 0.01) but there was no difference between groups with respect to IL-4, IL-5, IL-6, IL-12, TGF-beta, and eotaxin-2 and -3. The up-regulation of proinflammatory cytokines and chemokines related to the influx of phagocytic cells might account for the localisation of this infection to the upper respiratory tract. The up-regulation of the expression of the immunomodulatory cytokine IL-10 in nasal tissue from affected dogs might be important in limiting the extent of local tissue destruction, but might also account for the fact that infected dogs are generally unable to clear this infection spontaneously. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailReal-time RT-PCR quantification of mRNA encoding cytokines, CC chemokines and CCR3 in bronchial biopsies from dogs with eosinophilic bronchopneurnopathy
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Veterinary Immunology and Immunopathology (2006), 110(1-2), 65-77

Idiopathic canine eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the pulmonary interstitium and bronchial mucosa, a cause for which has not yet been ... [more ▼]

Idiopathic canine eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the pulmonary interstitium and bronchial mucosa, a cause for which has not yet been discovered. A recent study, examining the relative proportion of various lymphocyte cell subsets within bronchoalveolar lavage fluid from dogs with EBP, has shown a selective increase in CD4(+) T-cells and a selective decrease in CD8(+) T-cells, suggesting that a similar Th2 immune response might occur in EBP. The aim of the present study was to determine the profile of cytokine, chemokine and CC chemokine receptor 3 (CCR3) messenger RNA (mRNA) expression in bronchial tissue from dogs with EBP. Real-time RT-PCR assays were used for the quantification of mRNA encoding for a panel of cytokines, CC chemokines and CCR3 in perendoscopic bronchial biopsies from eight dogs with EBP and seven age-matched control dogs. Messenger RNA transcribed from the housekeeping gene glyceraldehyde-3 -phosphate dehydrogenase was used for normalisation of the threshold cycle in order to determine the relative copy numbers of the transcripts. No significant difference in the expression of any cytokine, MCP-1, -2, -4 and CCR3 was found between control and EBP dogs. The expression of transcript for MCP-3, eotaxin-2 and -3 was significantly greater in bronchial biopsies from dogs with EBP than in samples from control dogs while there was significantly less mRNA encoding RANTES in the mucosa of dogs with EBP. In conclusion, the cytokine mRNA expression profile in perendoscopic bronchial biopsies is similar in dogs with EBP and dogs without respiratory disease. Further studies on the quantification of mRNA encoding cytokines in isolated T lymphocytes from bronchoalveolar lavage fluid or bronchial biopsies are needed before any conclusion on the cytokine profile in canine EBP can be drawn. Eotaxin-2, -3 and MCP-3 appear to be implicated in the pathogenesis of the disease. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailOmega 3 fatty acid complementation (fish oil) in an experimental model of feline asthma : effect on bronchial reactivity, airway inflammation and oxidative stress markers
Kirschvinck, N.; Leemans, Jérôme ULg; Delvaux, F. et al

in Proceedings: 24th Symposium of the Veterinary Comparative Respiratory Society,”Respiratory infections and zoonoses (2006)

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See detailDiagnostic impact of thoracic radiography in an experimental model of feline asthma
Kirschvinck, N.; Kersnak, E.; Leemans, Jérôme ULg et al

in Proceedings: 24th Symposium of the Veterinary Comparative Respiratory Society,”Respiratory infections and zoonoses" (2006)

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See detailEffect of omega-3 fatty acid supplementation (fish oil) on bronchial reactivity, airway inflammation and oxidative stress markers in a feline model of asthma
Kirschvinck, N.; Leemans, Jérôme ULg; Delvaux, F. et al

in Proceedings: 16th Congress European College of Veterinary Internal Medicine – companion animals (2006)

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See detailBronchoprotective effect of inhaled salmeterol, salbutamol and ipratropium bromide using different devices on muscarinic bronchoconstriction in healthy cats
Leemans, Jérôme ULg; Kirschvinck, N.; Bernaerts, F. et al

in Proceedings: 16th Congress European College of Veterinary Internal Medicine – companion animals (2006)

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See detailSuspected drug-induced destructive cholangitis in a young dog.
Gabriel, Alexandra; van den Ingh, Ted; Clercx, Cécile ULg et al

in Journal of Small Animal Practice (2006), 47(6)

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See detailLaryngeal paralysis-polyneuropathy complex in young related Pyrenean Mountain dogs.
Gabriel, Alexandra; Poncelet, Luc; Van Ham, Luc et al

in Journal of Small Animal Practice (2006), 47(3)

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See detailProcollagen type-III aminoterminal peptide in serum and bronchoalveolar lavage fluid in dogs with chronic bronchopneumopathies – preliminary results
Schuller, S.; Talavera, J.; Jespers, P. et al

in 15th ESVIM Meeting - Glasgow - Ecosse - Septembre 2005 (2005, September)

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