References of "Clercx, Cécile"
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See detailWhole Blood and Tissue Fungal DNA Quantification in the Diagnosis of Canine Sino-Nasal Aspergillosis
Peeters, Dominique ULg; Peters, I. R.; Helps, C. R. et al

in Veterinary Microbiology (2008), 128(1-2), 194-203

Various combinations of tests are used to confirm the diagnosis of canine sino-nasal aspergillosis (SNA) because false-positive and false-negative results can occur with each test. Therefore, the aim of ... [more ▼]

Various combinations of tests are used to confirm the diagnosis of canine sino-nasal aspergillosis (SNA) because false-positive and false-negative results can occur with each test. Therefore, the aim of this study was to evaluate whether detection of fungal DNA in blood and nasal tissue samples was of value in the clinical diagnosis of this disease. Four groups were included in the study (dogs with SNA, lymphoplasmacytic rhinitis or nasal neoplasia, and control animals). Real-time PCR assays detecting DNA from all Penicillium and Aspergillus species (PenAsp assay) or species-specific DNA from A. fumigatus, A. terreus, A. flavus and A. niger were applied to whole blood and nasal tissue samples. Results obtained by PCR were compared between the groups. Sensitivity, specificity, positive and negative predictive values (PPV and NPV) for fungal DNA detection were compared with those for alternative diagnostic procedures including histopathology, serology and fungal culture. Significantly more fungal DNA was detected by the PenAsp assay in tissue biopsies from dogs with SNA than in the three other groups. Sensitivity, specificity, PPV and NPV for this method were 1.00, 0.06, 0.32 and 1.00. A. fumigatus DNA was detected in seven tissue biopsies from dogs with SNA and in one biopsy from a dog with a nasal tumour. Sensitivity, specificity, PPV and NPV for this diagnostic test were 0.50, 0.97, 0.87 and 0.82. No significant difference was found between the groups with respect to the amount of DNA detected in blood by the PenAsp assay. Sensitivity, specificity, PPV and NPV for this method were 0.71, 0.24, 0.31 and 0.64. A. fumigatus DNA was detected in the blood of three dogs with SNA and sixteen dogs without SNA. Sensitivity, specificity, PPV and NPV for this diagnostic tool were 0.21, 0.45, 0.15 and 0.54. Detection of A. fumigatus DNA in nasal tissue had the highest specificity, PPV and NPV but sensitivity of this method was low. Detection of fungal DNA in whole blood was of no value in the diagnosis of SNA. [less ▲]

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See detailEffect of fish oil supplementation in a model of a feline asthma
Kirschvink, N.; Leemans, Jérôme ULg; Delvaux, F. et al

in Acta Physiologica (2008), 192(662 Suppl), 10

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See detailReverse sneezing / rhinitis, bacterial / rhinitis, lymphoplasmacytic / sinusitis and other sinus disorders
Clercx, Cécile ULg

in Sellon, Rance (Ed.) The Veterinary Clinical Advisor (2008)

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See detailClinical and functional responses to inhaled albuterol, ipratropium bromide and combination of both in ascaris suum-sensitized cats with allergen-induced bronchospasm
Leemans, Jérôme ULg; Kirschvink, N.; Cambier, Carole ULg et al

in Proceedings: 26th Annual Symposium of the Veterinary and Comparative Respiratory Society (2008)

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See detailOral and inhaled corticosteroïds decreased eosinophilic airway inflammation and bronchial reactivity in ascaris suum-sensitized and challenged cats
Leemans, Jérôme ULg; Kirschvink, N.; Cambier, Carole ULg et al

in Proceedings: 26th Annual Symposium of the Veterinary and Comparative Respiratory Society (2008)

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See detailDietary supplementation with an omega-3 polyunsaturated fatty acids and luteolin combination in cats
Leemans, Jérôme ULg; Cambier, Carole ULg; Chandler, T. et al

in Proceedings: 26th Annual Symposium of the Veterinary and Comparative Respiratory Society (2008)

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See detailImmune-mediated respiratory and cardiac disease
Clercx, Cécile ULg

in Clinical Immunology of the Dog and Cat”, 2d edition (2008)

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See detailLe point sur le diagnostic de l’aspergillose naso-sinusale chez le chien.
Billen, Frédéric ULg; Clercx, Cécile ULg; Peeters, Dominique ULg

in Annales de Médecine Vétérinaire (2008), 152(2)

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See detailTherapeutic response in dogs with lymphoplasmacytic rhinitis: a retrospective study in 12 dogs
Manens, Jefferson ULg; Bolognin, M.; Mercier, Elise ULg et al

in 25th Symposium of the Veterinary Comarative Respiratory Society - Purdue, USA 10-13 octobre 2007 (2007, October 10)

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See detailActivity of metalloproteinases mmp2 and mmp9 in matched serum and bronchoalveolar lavage fluidsamples from dogs with chronic bronchitis cvresus recurrent infectious bronchopneumonia
Bolognin, M.; Kirschvink, N.; Wiggers, L. et al

in 17th ECVIM Meeting - Budapest - Hongrie - Septembre 2007 (2007, September 13)

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See detailPrimary ciliary dyskinesia in a family of Old English Sheepdogs
Billen, Frédéric ULg; Binst, Dominique; Geyskens, Valerie et al

in Proceedings of the 17th ECVIM-CA congress (2007, September)

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See detailUpdate on Canine Sinonasal Aspergillosis
Peeters, Dominique ULg; Clercx, Cécile ULg

in Veterinary Clinics of North America. Small Animal Practice (2007)

Sinonasal aspergillosis is a frequent cause of nasal discharge that occurs in otherwise healthy, young to middle-aged dogs. A local immune dysfunction is suspected in affected animals, and the role of ... [more ▼]

Sinonasal aspergillosis is a frequent cause of nasal discharge that occurs in otherwise healthy, young to middle-aged dogs. A local immune dysfunction is suspected in affected animals, and the role of increased interleukin-10 mRNA expression in the nasal mucosa of affected dogs is currently under investigation. Despite recent advances in imaging techniques, the "gold standard" for diagnosing the disease is direct visualization of fungal plaques during endoscopy or observation of fungal elements on cytology or histopathologic examination. Treatment can be challenging; however, the use of topical enilconazole or clotrimazole through noninvasive techniques has increased the success of treatment and decreased the morbidity and duration of hospitalization. [less ▲]

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See detailCanine eosinophilic bronchopneumopathy
Clercx, Cécile ULg; Peeters, Dominique ULg

in Veterinary Clinics of North America. Small Animal Practice (2007)

Eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the lung and bronchial mucosa, as demonstrated by examination of bronchoalveolar lavage fluid cytologic ... [more ▼]

Eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the lung and bronchial mucosa, as demonstrated by examination of bronchoalveolar lavage fluid cytologic preparations or histologic examination of the bronchial mucosa. Although the precise cause of EBP is unknown, a hypersensitivity to aeroallergens is suspected. The diagnosis relies on typical history and clinical signs, demonstration of bronchopulmonary eosinophilia by cytology or histopathologic examination, and exclusion of known causes of lower airway eosinophilia. Most dogs display an excellent response to oral corticosteroid therapy; however, side effects of this treatment can be limiting. New therapeutic approaches are being studied, including the use of aerosol therapy, cyclosporine, or drugs interfering with T helper 2 immune response. [less ▲]

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See detailInfluence of age on bronchoscopical findings in healthy beagle dogs
Mercier, Elise ULg; Bolognin, Myriam; Delvaux, François ULg et al

in Proceedings of the 17th Annual Congress of the ECVIM-CA (2007)

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See detailNon-invasive assessment of airway responsiveness in healthy and allergen-sensitised cats by use of barometric whole body plethysmography
Kirschvink, N.; Leemans, Jérôme ULg; Delvaux, François et al

in Veterinary Journal (2007), 173(2), 343-352

This study aimed at determining whether airway responsiveness (AR) tests performed by use of barometric whole body plethysmography (BWBP) were repeatable in cats and to what extent AR was affected by the ... [more ▼]

This study aimed at determining whether airway responsiveness (AR) tests performed by use of barometric whole body plethysmography (BWBP) were repeatable in cats and to what extent AR was affected by the nebulization protocol used, the age of the animals, the inflammatory status of the airways and prior bronchodilator treatment. Repeatability of AR was tested on two occasions in 30 healthy cats. The concentration of carbachol inducing a 300% increase of the enhanced pause (Penh) - an estimator of airflow limitation - was calculated (C-Penh300) and did not differ significantly between the two tests (0.035 +/- 0.017% compared to 0.034 +/- 0.016%) and was significantly and positively correlated. The comparison between rapidly and slowly increasing carbachol concentrations was performed in ten healthy cats and showed a significantly lower C-Penh300 (%) when slowly increasing concentrations were used (0.037 +/- 0.013% compared to 0.039 +/- 10.015%, P < 0.05). A significant age-related increase of C-Penh300 was evidenced by performing AR tests in 15 healthy cats at 12, 18, 24 and 30 months (12 months: 0.026 +/- 0.008%, 18 months: 0.031 +/- 0.009%, 24 months: 0.038 +/- 0.01%, 30 months: 0.043 +/- 0.014%, P < 0.05). C-Penh300 significantly decreased in 12 Ascaris suum-sensitised cats after allergen exposure (0.026 +/- 0.016% compared to 0.033 +/- 0.016%, P < 0.05) and was negatively correlated with the granulocyte percentage of bronchoalveolar lavage fluid (r = -0.36, P < 0.01). Compared with a placebo inhalation, pretreatment with inhaled salbutamol significantly increased C-Penh300 in four healthy cats (0.093 +/- 0.021% compared to 0.036 +/- 0.004%, P < 0.05). This study provides evidence that AR determination by use of BWBP is promising as non-invasive indicator of lower airway inflammation or for monitoring response to bronchodilator treatment. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailEffects of age and allergen-induced airway inflammation in cats: Radiographic and cytologic correlation
Kirschvink, Nathalie; Kersnak, Emilie; Leemans, Jérôme ULg et al

in Veterinary Journal (2007), 174(3), 644-651

Thoracic radiography is an important diagnostic tool for feline respiratory medicine. The aim of this study was (1) to assess agerelated changes of thoracic radiographic views in healthy young cats and (2 ... [more ▼]

Thoracic radiography is an important diagnostic tool for feline respiratory medicine. The aim of this study was (1) to assess agerelated changes of thoracic radiographic views in healthy young cats and (2) to test if experimentally-induced bronchial inflammation by inhaling Ascaris suum (AS) allergens leads to radiographic changes after single or repeated exposures. Healthy cats (n = 15-30) aged between 6 and 30 months were evaluated. Eight healthy cats and eight AS-sensitised cats, respectively, inhaled sterile saline or allergen. Radiographs were taken 24 h before, and 6, 24 and 48 h after the challenge. Bronchoalveolar lavage (BAL) was performed after the last radiographic examination. AS-sensitised cats underwent three further allergen challenges at 3-month intervals. The radiographic evaluation was based on a scoring system considering bronchial, interstitial and alveolar patterns. A significant age-related increase in interstitial and total radiographic score was detected in healthy cats older than 18 months and in healthy cats older than 24 months. Whilst saline inhalation did not affect radiographic scores, a single AS challenge induced significant changes of all scores within 6-24 h. A significant positive correlation between radiographic scores and BAL neutrophils and eosinophils was found. Repeated AS challenges did not induce irreversible changes in radiographic scores. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailFunctional, inflammatory and morphological characterisation of a cat model of allergic airway inflammation
Kirschvink, N.; Leemans, Jérôme ULg; Delvaux, François et al

in Veterinary Journal (2007), 174(3), 541-553

The aims of this study were to characterise a model of feline allergic airway inflammation and to test through a longitudinal investigation whether five repeated allergen exposures would lead to signs of ... [more ▼]

The aims of this study were to characterise a model of feline allergic airway inflammation and to test through a longitudinal investigation whether five repeated allergen exposures would lead to signs of airway remodelling that would be detectable in vivo. Eight healthy control cats and eight cats sensitised with Ascaris suum allergens were investigated. Barometric whole body plethysmography (BWBP) was used for the assessment of respiratory variables and airway responsiveness (AR). Bronchoalveolar lavage fluid (BALF) was sampled for cytology and determination of F-2-isoprostane concentration and matrix metalloproteinase type 9 (MMP-9) activity. Thoracic radiography and bronchoscopy scores were also established. Cats were investigated prior to sensitisation and after inhalation of placebo or allergen challenge 1. BWBP measurements revealed a significant increase of enhanced pause (Penh), an index of bronchoconstriction, and AR in sensitised cats in response to allergen challenge 1. A significant increase in BALF neutrophil and eosinophil %, F-2-isoprostane concentration and MMP-9 activity, and increased radiography and bronchoscopy scores were recorded. After a recovery period of 6 weeks, all variables except BALF MMP-9 returned to baseline values. Four further allergen challenges induced similar changes to those seen in challenge I and no signs of persistent changes suggestive of bronchial remodelling were detectable. The model provides an in vivo approach to functional, inflammatory and morphological changes occurring in response to single and repeated allergen exposure. [less ▲]

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See detailLong-term outcome in dogs with sinonasal aspergillosis treated with intranasal infusions of enilconazole
Schuller, S.; Clercx, Cécile ULg

in Journal of the American Animal Hospital Association (2007)

Long-term outcomes (mean 38+/-17 months) were evaluated in 27 dogs with sinonasal aspergillosis after successful medical treatment using intranasal infusions of 1% or 2% enilconazole (1%, n=15; 2%, n=12 ... [more ▼]

Long-term outcomes (mean 38+/-17 months) were evaluated in 27 dogs with sinonasal aspergillosis after successful medical treatment using intranasal infusions of 1% or 2% enilconazole (1%, n=15; 2%, n=12). Long-term outcomes with both treatment protocols were good, with half of the dogs being asymptomatic throughout the follow-up period. The remaining dogs showed mild clinical signs compatible with chronic rhinitis/sinusitis. These clinical signs were interpreted as chronic lymphoplasmacytic rhinitis/sinusitis and episodes of bacterial rather than fungal infection. Three dogs had confirmed reinfection or relapse 2 to 36 months after clinical resolution [less ▲]

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