Showing results 241 to 260 of 293
 Evaluation of some parameters influencing the drug delivery from a dry powder inhalation device using an in vitro model of the horse airways; Chiap, Patrice  ; Crommen, Jacques et alin Veterinary Research (1997), 28(6), 557-564 The aim of this study was to determine the effect of breathing pattern, air humidity and position of the device on the delivery of an aerosol generated by a dry powder inhalation (DPI) device (Inhalator M ... [more ▼] The aim of this study was to determine the effect of breathing pattern, air humidity and position of the device on the delivery of an aerosol generated by a dry powder inhalation (DPI) device (Inhalator M). The in vitro inhalation study was performed using the cascade impaction method (Andersen Sampler) adapted to imitate nasal breathing. The amount of ipratropium found in the device, the artificial upper airways and the six stages of the Andersen Sampler was measured using high precision liquid chromatography. Stage 1 of the Andersen Sampler was considered to be the respirable fraction and stages 2 to 6 to be the non-respirable fraction. It was concluded that the theoretical respirable fraction of ipratropium obtained after DPI through Inhalator M was influenced by relative air humidity, air flow and the position of the device, whereas the number of successive inspirations and the duration of inspiration did not affect this fraction of the drug. [less ▲] Detailed reference viewed: 1 (0 ULg)Coupled-column LC analysis of mephobarbital enantiomers in plasma using restricted access packing material in the precolumnCeccato, Attilio ; Chiap, Patrice ; Hubert, Philippe et alConference (1997) Detailed reference viewed: 7 (0 ULg)Automated liquid chromatographic determination of atenolol in plasma using dialysis and trace enrichment on a cation exchange precolumn for sample pre-treatementChiap, Patrice ; ; Ceccato, Attilio et alPoster (1997) Detailed reference viewed: 9 (0 ULg)Méthodes chromatographiques de dosage dans les milieux biologiques : Stratégie de validation; Boulanger, Bruno ; et alConference (1997) Detailed reference viewed: 55 (0 ULg)Fully automated systems for the liquid chromatographic determination of drug enantiomers in biological fluidsCrommen, Jacques ; Ceccato, Attilio ; et alConference (1997) Detailed reference viewed: 3 (0 ULg)Selecting Derringer’s desirability functions that guarantee Pareto optimality : an application to the optimization of a liquid chromatography separationBoulanger, Bruno ; Ceccato, Attilio ; Chiap, Patrice et alPoster (1997) Detailed reference viewed: 21 (0 ULg)LC determination of mephobarbital enantiomers and phenobarbital in plasma using a precolumn with restricted access sorbent for sample clean-upCeccato, Attilio ; Boulanger, Bruno ; Chiap, Patrice et alPoster (1997) Detailed reference viewed: 4 (0 ULg)Automated LC determination of molsidomine and two of its metabolites in plasma using solid phase extraction as sample pretreatmentChiap, Patrice ; Hubert, Philippe ; Ceccato, Attilio et alPoster (1997) Detailed reference viewed: 3 (0 ULg)On-line combination of dialysis with trace enrichment on a cation-exchange precolumn for the automated LC determination of sotalol in plasmaChiap, Patrice ; Ceccato, Attilio ; et alPoster (1997) Detailed reference viewed: 3 (0 ULg) Méthodes chromatographiques de dosage dans les milieux biologiques : stratégie de validation - Rapport d'une commission SFSTP; ; et al in STP Pharma Pratiques (1997), 7(3), 169-194 Detailed reference viewed: 92 (13 ULg)Automated Determination of Verapamil and Norverapamil in Human Plasma with on-Line Coupling of Dialysis to High-Performance Liquid Chromatography and Fluorometric DetectionCeccato, Attilio ; Chiap, Patrice ; Hubert, Philippe et alin Journal of Chromatography. A (1996), 750(1-2), 351-60 A fully automated method for the simultaneous determination of verapamil and its main metabolite norverapamil in human plasma is described. This method is based on on-line sample preparation using ... [more ▼] A fully automated method for the simultaneous determination of verapamil and its main metabolite norverapamil in human plasma is described. This method is based on on-line sample preparation using dialysis followed by clean-up and enrichment of the dialysate on a precolumn and subsequent HPLC analysis with fluorometric detection. All sample handling operations were performed automatically by a sample processor equipped with a robotic arm (ASTED system). The plasma samples were dialysed on a cellulose acetate membrane (cut-off: 15 kD) and the dialysate was purified and enriched on a short pre-column filled with cyanopropyl silica. Before starting dialysis, this trace enrichment column (TEC) was first conditioned with the HPLC mobile phase and then with pH 3.0 acetate buffer. 370 microliters of plasma sample spiked with the internal standard (gallopamil) were dialysed in the static-pulsed mode. The solution at the donor side was pH 3.0 acetate buffer containing Triton X-100 while the acceptor solution was made of the same acetate buffer. When dialysis was discontinued, the analytes were desorbed from the TEC by the HPLC mobile phase and transferred to the C18 analytical column by means of a switching valve. This mobile phase consisted of a mixture of acetonitrile, pH 3.0 acetate buffer and 2-aminoheptane. The influence of different parameters of the dialysis process on the recovery of verapamil and norverapamil has been studied. The effect of the volume, the aspirating and dispensing flow-rates of the dialysis solution has been investigated. The recoveries of verapamil and norverapamil in plasma were close to 75% and the limits of quantification were 5 ng/ml for both analytes. The method was found to be linear in the concentration range from 5 to 500 ng/ml (r2: 0.9996 for both analytes). The intra-day and inter-day reproducibilities at a concentration of 100 ng/ml were 2.3% and 5.6% for verapamil and 1.7% and 5.1% for norverapamil, respectively. [less ▲] Detailed reference viewed: 9 (0 ULg)Method validation in a European context, Chromatografische informatie in cyberspace en in andere moderne mediaHubert, Philippe ; Chiap, Patrice ; Crommen, Jacques Conference (1996) Detailed reference viewed: 7 (2 ULg)On-line dialysis coupled to liquid chromatography for the automated determination of verapamil and its demethylated metabolite in plasmaCeccato, Attilio ; Hubert, Philippe ; Chiap, Patrice et alConference (1996) Detailed reference viewed: 2 (1 ULg)Analytical technologies and tolls for drug purity testingCrommen, Jacques ; Chiap, Patrice ; Ceccato, Attilio et alConference (1996) Detailed reference viewed: 2 (0 ULg)Strategy for bioanalytical method validation : approach of SFSTP commissionHubert, Philippe ; Chiap, Patrice ; Crommen, Jacques Conference (1996) Detailed reference viewed: 6 (0 ULg)Méthodes chromatographiques de dosage dans les milieux biologiques : Stratégie de validation; Boulanger, Bruno ; et alConference (1996) Detailed reference viewed: 6 (2 ULg)LC determination of oxprenolol enantiomers in human plasma using dialysis as on-line sample preparation techniqueCeccato, Attilio ; ; Chiap, Patrice et alin Journal de Pharmacie de Belgique (1996), 51 Detailed reference viewed: 2 (1 ULg)On-line dialysis coupled to liquid chromatography for the automated determination of verapamil and its demethylated metabolite in plasma, chapitre "biomedical analysis with hyphenated chromatography"Ceccato, Attilio ; Hubert, Philippe ; Chiap, Patrice et alin Hyphenated techniques in chromatography and hyphenated chromatographic analysers (1996) Detailed reference viewed: 11 (6 ULg)On-line dialysis coupled to liquid chromatography for the automated determination of oxprenolol enantiomers in human plasmaCeccato, Attilio ; ; et alPoster (1996) Detailed reference viewed: 4 (1 ULg) |
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