References of "Chapelle, Jean-Paul"
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See detailRace cycling: biological evolution
LE GOFF, Caroline ULg; Kaux, Jean-François ULg; Goffaux, Sébastien et al

in Meeusen, R; Duchateau, J; Roelands, B (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

Introduction: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and ... [more ▼]

Introduction: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and metabolic) released during an international cycling race. Materials and methods: Venous blood samples of 15 young men (25.1 ± 6.4 y.o.) were collected just before (T1), just after (T2), 3 hours (T3) after an international cycling race of 179.6 kilometers in Belgium for the determination of cardiac and metabolic biomarkers: red blood cell (RBC), haemoglobin (HgB), creatinin (Cr), highly sensitive troponin T (hsTnT), myoglobin (MYO) and NT-proBNP. All automated assays were performed according to the manufacter’s specifications. For the statistical analysis, an Anova calculated with the Statistica Software version 9.1 was used. Results and discussions: RBC and HgB levels varied significantly between T0 and T3 (respectively p=0.0026, and p=0.002). Cr concentration also varied significantly between all times (T0-T1:p<0.0001, T1-T3:p=0.0326 and T0-T3 p=0.0001). These changes might be related to renal flow depletion during exercice. MYO increased significantly between T0 and T1 (p<0.0001), but quickly decreased between T1 and T3, however the T3 level stay higher than T0 (p=0.014). The stress delivered from the physical activity performed during the race induced a significant variation of hsTnT which increased significantly between T0 and T1 (p<0.0001) and stayed higher 3 hours after the end of the exercise (T0-T3: p<0.0001). The intense exercise delivery by the race induced a significant variation of NT-proBNP, that followed the same kinetic of hsTnT but in smaller proportion. We noticed variations statistically significant between T0 and T1 and between T0 and T3 for NT-proBNP. These increases of cardiac biomarkers were significant but reasonable and could not allow us to talk about cellular necrosis or irreversible injury. Conclusion: Our results show that stress generated by a cycling race could be the cause for the different metabolic variations observed. Troponin T stays without a doubt the most specific marker for stress related to myocardial tissue. Its increase can then be considered as being of interest. [less ▲]

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See detailImpact of strenous exercise on the release of cardiac biomarkers
LE GOFF, Caroline ULg; MELON, Pierre ULg; Kaux, Jean-François ULg et al

in Meeusen, R; Duchateau, J; Roelands, B (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured ... [more ▼]

Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured by new commercially available high-sensitive methods in subjects undergoing the Maasmarathon. Our aim was to compare cTnT and NT-proBNP levels in sportive subjects before and after a strenuous exercise. Materials and Methods: Twenty eight subjects (26 ♂, 42.5±11yo) underwent a race of 42.195 kilometers between Visé (Belgium) and Maastricht (The Netherlands). We drowned blood samples before (T0), just after (T1) and three hours after the race (T3). For all patients, cTnT concentrations were measured by high sensitive methods (hsTnT, Roche Diagnostics) on heparin plasma. The NT-proBNP was also determined with the kit Roche on heparin plasma. The protocol was approved by the ethics committee of the University of Liège (Belgium). All subjects gave their informed consent. All statistical analyses were performed using Medcalc version 8.1 for Windows. p-value <0.01 was regarded as statistically significant. Results and discussion: A significant difference between hsTnT concentrations at T0 and T1 (p<0.0001) was measured as well as between T0 and T3 (p<0.001) for NT-proBNP, but not between T1 and T3. This observation appeared only after a strenuous exercise but today this type of exercise is not reproduce easier in a laboratory of sport. Moreover, at this moment, nobody knows if these observations would have cardiac consequences at long terms. Conclusions: Measurement of cardiac troponins by high sensitive methods allows detecting significant release of biomarkers from the heart during exercise. The levels of NT-proBNP were found significantly increased but in less extent than TnThs. We think that the TnThs could be an interesting marker in the future to help sport medicine to detect risk of developing a cardiac problem. [less ▲]

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See detailSeasonal variations in vitamin D levels in melanoma patients: a single-centre prospective pilot comparative study
FAILLA, Valérie ULg; CAVALIER, Etienne ULg; EL HAYDERI, Lara ULg et al

in Journal of the European Academy of Dermatology & Venereology (2012), 26(5), 651-3

BACKGROUND: More than 90% of vitamin D synthesis is dependent on UV exposure. Photosensitive disorders such as lupus erythematosus, protoporphyria and xeroderma require strict sun avoidance, and vitamin D ... [more ▼]

BACKGROUND: More than 90% of vitamin D synthesis is dependent on UV exposure. Photosensitive disorders such as lupus erythematosus, protoporphyria and xeroderma require strict sun avoidance, and vitamin D deficiency has been demonstrated in these patients. Melanoma patients are also instructed to avoid sun exposure and may hence be expected to be vitamin D deficient. MATERIALS AND METHODS: Winter and summer vitamin D levels were compared in a group of melanoma patients (n =61) and age- and phototype-matched controls (n = 53) without photosensitive disorders. RESULTS: Oral supplementary vitamin D intake was reported in 32.7% of the melanoma patients and in 15.1% in the control group. Despite oral supplementation, only 25% of the melanoma patients and the controls presented with vitamin D levels of 30 ng/mL or higher. In non-supplemented subjects in the melanoma and control groups, respectively, mean winter vitamin D levels were below the recommended threshold at 12.6 ng/mL vs. 13.2 ng/mL, respectively, but not statistically different. These values increased significantly in both groups during the summer to 24.6 and 23.8 ng/mL respectively. CONCLUSION: Unexpected, significant increases in vitamin D levels were seen in melanoma patients during summer, suggesting non-adherence with photoprotective measures and reflecting a heliophilic behaviour. Vitamin D supplementation is recommended in melanoma patients during both winter and summer. [less ▲]

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See detailBiological variations during a race cycling
LE GOFF, Caroline ULg; Kaux, Jean-François ULg; Goffaux, Sébastien et al

in Biomedica 2012 (2012, April)

Background: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and ... [more ▼]

Background: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and metabolic) released during an international cycling race. Materials and Methods: Venous blood samples of 15 young men (25.1 ± 6.4 y.o.) were collected just before (T1), just after (T2), 3 hours (T3) after an international cycling race of 176 kilometers in Belgium for the determination of cardiac and metabolic biomarkers: red blood cells (RBC) haemoglobin (HgB) creatinin (Cr) highly sensitive troponin T (hsTnT) myoglobin (MYO) NT-proBNP All automated assays were performed according to the manufacter’s specifications. For the statistical analysis, an Anova calculated with the Statistica Software version 9.1 was used. Results: •RBC and HgB levels varied significantly between T0 and T3 (respectively p=0.0026, and p=0.002) (Fig. 1 and 2). • Cr concentration also varied significantly between all times (T0-T1:p<0.0001, T1-T3:p=0.0326 and T0-T3 p=0.0001)(Fig.3). These changes might be related to renal flow depletion during exercise. •MYO increased significantly between T0 and T1 (p<0.0001), but quickly decreased between T1 and T3, however the T3 level stay higher than T0 (p=0.014) (Fig.4). •The stress delivered from the physical activity performed during the race induced a significant variation of hsTnT which increased significantly between T0 and T1 (p<0.0001) and stayed higher 3 hours after the end of the exercise (T0-T3: p<0.0001) (Fig.5) . •The intense exercise delivery by the race induced a significant variation of NT-proBNP, that followed the same kinetic of hsTnT but in smaller proportion. We noticed variations statistically significant between T0 and T1 and between T0 and T3 for NT-proBNP (Fig.6). •These increases of cardiac biomarkers were significant but reasonable and could not allow us to talk about cellular necrosis or irreversible injury. Conclusions: Our results show that stress generated by a cycling race could be the cause for the different metabolic variations observed. Troponin T stays without a doubt the most specific marker for stress related to myocardial tissue. Its increase can then be considered as being of interest. [less ▲]

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See detailLe dosage des acides gras érythrocytaires : comparaison entre une population de référence et des sujets ayant présenté un infarctus aigu du myocarde
LE GOFF, Caroline ULg; Leroy, Ludovic; Kaux, Jean-François ULg et al

Poster (2012, February)

Introduction Un acide gras (AG) est un acide carboxylique avec une longue chaine aliphatique, qui peut être saturé ou insaturé. Récemment, le rôle des AG, particulièrement, celui des oméga-3 et oméga-6, a ... [more ▼]

Introduction Un acide gras (AG) est un acide carboxylique avec une longue chaine aliphatique, qui peut être saturé ou insaturé. Récemment, le rôle des AG, particulièrement, celui des oméga-3 et oméga-6, a émergé comme facteur de risque cardiovasculaire dans la littérature. Le but de ce travail était de comparer les taux d’AG mesurés dans une population saine avec ceux obtenus chez des patients admis aux urgences pour un infarctus du myocarde. Matériels et méthodes Cinquante quatre sujets sains (33±11ans, 31 femmes) ont été sélectionnés comme population de référence. Ces derniers ont été sélectionnés selon leur mode de vie (alimentation, tabac, etc). En parallèle, nous avons dosé les AG chez 33 patients (55±9 ans, 12 femmes) admis dans le service d’urgence de notre institution pour infarctus aigu du myocarde (AMI). Le sang était prélevé sur EDTA. Avant l’analyse, les échantillons étaient lavés et transméthylés. Le dosage des AG était réalisé par chromatographie gazeuse couplée à un détecteur à ionisation de flamme (GCFID). Après le dosage, nous avons réalisés une comparaison des taux, sommes et rapport entre les différents AG ainsi que l’index oméga-3 (somme de l’acide eicosapentaénoïque et docosahexaénoïque) obtenus dans les 2 groupes. Résultats. Des valeurs de référence ont été obtenues pour notre population de référence via le logiciel de statistique MedCalc. Dans le groupe AMI, les taux d’oméga-6 étaient significativement plus élevés (p<0.05) pour C18:2n6 (Figure 1) et C18:3n6 (Figure 2)comparés aux résultats obtenus dans la population de référence. Par contre les taux d’oméga-3 étaient significativement plus bas (p<0.01) par rapport aux sujets de référence pour le C22 :6n3 (Figure 3). L’index oméga-3 montrait une valeur plus basse et le rapport oméga-6/oméga-3 était plus élevé dans le groupe AMI comparé aux sujets de référence. Conclusion Le dosage des AG est un nouvel outil que le laboratoire peut proposer aux cliniciens afin de stratifier les patients présentant le plus de risque cardiovasculaire avant ou après un infarctus du myocarde. Ces patients pourraient ainsi être supplémenté en acides gras oméga-3 afin d’éviter les récidives d’infarctus du myocarde ou de diminuer la formation de la plaque d’athérosclérose avant le premier accident. [less ▲]

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See detailApport des troponines T et I ultrasensibles dans le diabète
LE GOFF, Caroline ULg; LAURENT, Terry ULg; GARWEG, Christophe ULg et al

in Immuno-Analyse & Biologie Spécialisée [=IBS] (2012, February), 27(1), 40

Introduction : Le diabète atteint environ 3% de la population française. Or, cette maladie expose à l'apparition précoce de complications cardiovasculaires. Les troponines de nouvelles générations ... [more ▼]

Introduction : Le diabète atteint environ 3% de la population française. Or, cette maladie expose à l'apparition précoce de complications cardiovasculaires. Les troponines de nouvelles générations hautement sensibles pourraient être un bon outil diagnostic pour mettre en évidence des macroou micro-angiopathies non diagnostiquée chez ces patients. Le but de notre étude était de comparer la performance de la troponine T ultrasensible (hsTnT) (Roche Diagnostic) avec la troponine I ultrasensible (TnI II) (Abbott Diagnostic) chez le sujet diabétique. Matériel et méthodes : Vingt patients diabétiques (âge moyen : 52.6 ± 8.4 ans) ont été sélectionnés sur le critère d’un taux d’hémoglobine glyquée (HbA1c) élevé. Ces sujets ont été comparés à vingt sujets contrôles d’âge moyen : 60.05 ± 2.86 ans. Les patients atteints d’insuffisance rénale et d’affections cardiovasculaires ont été exclus. Les analyses ont été réalisées sur du plasma hépariné lithium. La hsTnT a été dosée par électrochemiluminescence sur le Modular E (Roche Diagnostic). Abbott utilise des microparticules chemiluminescentes pour le dosage de la TnI II sur l’ARCHITECT i. Résultats : Les sujets diabétiques ont un taux plasmatique de hsTnT représenté par les valeurs suivantes [médiane (1er quartile, 3ème quartile)] : 0,007 (0,03 ; 0,018) ng/mL ; alors que les sujets contrôles négatifs présentent les valeurs suivantes : 0,003 (0,003 ; 0,004) ng/mL. La différence de taux de hsTnT observée entre ces 2 populations est statistiquement significative (p = 0,000922). Pour la TnI II, on observe un taux plasmatique de: 0,004 (0,003 ; 0,075) ng/mL chez les sujets diabétiques et 0,002 (0,001 ; 0,005) ng/mL chez les sujets contrôles. La différence de taux de TnI II observée entre ces 2 populations n’est pas statistiquement significative (p > 0,005). Discussion-conclusion : Au décours d’un diabète, il apparait que les taux de hsTnT sont augmentés de façon significative par rapport au groupe des contrôles négatifs. Si les taux hsTnT augmentent chez le diabétique, il semblerait normal qu’il en soit de même pour la TnI II, vu que ces deux marqueurs sont intimement liés. Nous ne l’avons pas observé mais ceci peut être du à la plus faible performance analytique du dosage de la TnI II utilisé. Cette libération de hsTnT dans le sang peut être due aux micro- et macro-angiopathies au niveau des coronaires induites au cours d’un diabète. [less ▲]

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See detailIntense physical exercise related to the emergent generation of cardiovascular risk markers: a review
LE GOFF, Caroline ULg; LAURENT, Terry ULg; Kaux, Jean-François ULg et al

in Biology of Sport (2012), 29(1), 11-16

Objective: The present review was performed in order to bring together the current knowledge about the impact of intense physical exercise on cardiovascular function, especially on plasma levels of ... [more ▼]

Objective: The present review was performed in order to bring together the current knowledge about the impact of intense physical exercise on cardiovascular function, especially on plasma levels of cardiovascular risk markers such as cardiac troponin T (cTnT), myeloperoxidase (MPO), amino-terminal pro-brain natriuretic peptide (NT-proBNP), C-reactive protein (CRP) and oxidized low-density lipoprotein (oxLDL). Methods: Data were collected using the PubMed database. The articles were chosen for their relevance and importance in the area of interest. Results: The literature describes numerous examples where physical exercise induces plasma variation for the markers studied. Intense physical effort increases the levels of cTnT, MPO and NTproBNP, whereas CRP and oxLDL levels tend to be decreased with regular sport activities. Conclusions: The present literature investigation confirms the fact that intense physical exercise has an impact on the plasma variations of the five cardiovascular risk markers studied. However, practising regular exercise remains one of the first strategies for the prevention of cardiovascular disorders. [less ▲]

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See detailProcalcitonin usefulness for the initiation of antibiotic treatment in intensive care unit patients.
LAYIOS, Nathalie ULg; LAMBERMONT, Bernard ULg; CANIVET, Jean-Luc ULg et al

in Critical Care Medicine (2012), 40(8), 2304-9

OBJECTIVES: : To test the usefulness of procalcitonin serum level for the reduction of antibiotic consumption in intensive care unit patients. DESIGN: : Single-center, prospective, randomized controlled ... [more ▼]

OBJECTIVES: : To test the usefulness of procalcitonin serum level for the reduction of antibiotic consumption in intensive care unit patients. DESIGN: : Single-center, prospective, randomized controlled study. SETTING: : Five intensive care units from a tertiary teaching hospital. PATIENTS: : All consecutive adult patients hospitalized for > 48 hrs in the intensive care unit during a 9-month period. INTERVENTIONS: : Procalcitonin serum level was obtained for all consecutive patients suspected of developing infection either on admission or during intensive care unit stay. The use of antibiotics was more or less strongly discouraged or recommended according to the Muller classification. Patients were randomized into two groups: one using the procalcitonin results (procalcitonin group) and one being blinded to the procalcitonin results (control group). The primary end point was the reduction of antibiotic use expressed as a proportion of treatment days and of daily defined dose per 100 intensive care unit days using a procalcitonin-guided approach. Secondary end points included: a posteriori assessment of the accuracy of the infectious diagnosis when using procalcitonin in the intensive care unit and of the diagnostic concordance between the intensive care unit physician and the infectious-disease specialist. MEASUREMENTS AND MAIN RESULTS: : There were 258 patients in the procalcitonin group and 251 patients in the control group. A significantly higher amount of withheld treatment was observed in the procalcitonin group of patients classified by the intensive care unit clinicians as having possible infection. This, however, did not result in a reduction of antibiotic consumption. The treatment days represented 62.6 +/- 34.4% and 57.7 +/- 34.4% of the intensive care unit stays in the procalcitonin and control groups, respectively (p = .11). According to the infectious-disease specialist, 33.8% of the cases in which no infection was confirmed, had a procalcitonin value >1microg/L and 14.9% of the cases with confirmed infection had procalcitonin levels <0.25 microg/L. The ability of procalcitonin to differentiate between certain or probable infection and possible or no infection, upon initiation of antibiotic treatment was low, as confirmed by the receiving operating curve analysis (area under the curve = 0.69). Finally, procalcitonin did not help improve concordance between the diagnostic confidence of the infectious-disease specialist and the ICU physician. CONCLUSIONS: : Procalcitonin measuring for the initiation of antimicrobials did not appear to be helpful in a strategy aiming at decreasing the antibiotic consumption in intensive care unit patients. [less ▲]

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See detailMulticentre Evaluation of a Second Generation Point-of-Care Assay with an Extended Range for the Determination of N-Terminal Pro-Brain Natriuretic Peptide
Jorgensen, Bo; Bertsch, Thomas; Bröker, Hans-Joachim et al

in Clinical Laboratory (2012), 58

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See detailInterpretation of serum PTH concentrations with different kits in dialysis patients according to the KDIGO guidelines: importance of the reference (normal) values
CAVALIER, Etienne ULg; DELANAYE, Pierre ULg; VRANKEN, Laura ULg et al

in Nephrology Dialysis Transplantation (2012), 27

Background. The recommended target range for serum parathyroid hormone (PTH) in dialysis patients has changed from 150 to 300 pg/mL in the KDOQI guidelines to two to nine times the upper normal limit in ... [more ▼]

Background. The recommended target range for serum parathyroid hormone (PTH) in dialysis patients has changed from 150 to 300 pg/mL in the KDOQI guidelines to two to nine times the upper normal limit in the KDIGO ones. Although inclusion/exclusion criteria for the reference population are highly important, they are usually not mentioned in the commercial kits. In this study, we used the same reference population of vitamin D-replete normal subjects to establish reference values for 10 commercial PTH kits. We evaluated whether this may improve the classification of dialysis patients according to the KDIGO compared to the use of reference values proposed by the manufacturers. Methods. We measured serum PTH with 10 different kits in 149 haemodialysis patients, and 240 25-OH-vitamin D-replete (>75 nmol/L) individuals with an estimated glomerular filtration rate >60 mL/min/1.73 m2. Results. For the 10 kits, our upper normal limit was lower than those of the manufacturers. The difference was, however, variable from one kit to another. The two kits that yielded the lowest and the highest absolute concentrations classified differently 84/149 patients (56.4%) according to the KDOQI and 53/149 (36.2%) according to the KDIGO using the manufacturers’ normal value.Using our normal values significantly decreased the discrepancies with 24/149 patients (16.1%) being still classified differently. Taking the measurement uncertainty into consideration, 8% of the patients only remained differently classified by these two kits. Conclusions. Using the same vitamin-D-replete population to establish the reference range for 10 commercial PTH kits significantly improved the classification of haemodialysis patients according to the KDIGO target range. [less ▲]

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See detailAnalytical evaluation of the new Abbott Architect 25-OH vitamin D assay
CAVALIER, Etienne ULg; CARLISI, Ignazia ULg; BEKAERT, Anne-Catherine ULg et al

in Clinical Biochemistry (2012), 45

Objectives: Validation of the Architect 25-OH vitamin D assay. Design and methods: Determination of repeatability, reproducibility, accuracy profile and 25(OH)-vitamin D2 recovery on native samples ... [more ▼]

Objectives: Validation of the Architect 25-OH vitamin D assay. Design and methods: Determination of repeatability, reproducibility, accuracy profile and 25(OH)-vitamin D2 recovery on native samples. Comparison with DiaSorin Liaison and RIA. Results and conclusion: Coefficients of variation: b6% (13.6 ng/mL) and 2.2% (78.1 ng/mL). Functional sensitivity: 5 ng/mL. Accuracy profile shows that the method is validated between 13.6 and 78.1 ng/mL. Recovery of 25(OH)D2: 75,8%( 95% CI: 61.9–89.7%). Good correlation with DiaSorin RIA and Liaison b50 ng/mL; above this threshold a systematic positive bias was observed. [less ▲]

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See detailBNP et NT-proBNP: valeurs de référence et seuils décisionnels
TEIXEIRA, Jelda ULg; Guillaume, Michèle ULg; NELLESSEN, Eric ULg et al

in Revue Médicale de Liège (2012), 67(1), 38-43

Les peptides natriurétiques, en particulier BNP et NT-proBNP, sont de plus en plus utilisés comme tests de screening chez les patients avec suspicion d’insuffisance cardiaque (IC), afin d’éviter de ... [more ▼]

Les peptides natriurétiques, en particulier BNP et NT-proBNP, sont de plus en plus utilisés comme tests de screening chez les patients avec suspicion d’insuffisance cardiaque (IC), afin d’éviter de recourir d’emblée à des examens spécialisés coûteux tels que l’échocardiographie. Très performants pour la valeur prédictive élevée d’un résultat négatif, permettant ainsi d’exclure l’IC chronique avec une forte probabilité, ces biomarqueurs sont également reconnus pour leur intérêt diagnostique dans cette pathologie. Des taux élevés de peptides natriurétiques sont corrélés avec un risque accru d’hospitalisation pour cause cardiovasculaire et de décès. La stratification du risque chez les patients présentant une insuffisance cardiaque est facilitée par l’utilisation de seuils décisionnels «bas» et «élevé», pour lesquels différentes valeurs ont été proposées dans la littérature. Le présent article a pour objet de faire le point sur le positionnement des seuils décisionnels eu égard aux valeurs de référence de NT-proBNP déterminées dans la population résidant en Province de Liège (Belgique). Les données ont été analysées en fonction de l’âge et du sexe des sujets, deux des facteurs majeurs de variation des concentrations plasmatiques des peptides natriurétiques. [less ▲]

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