References of "Cavalier, Etienne"
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See detailMulticenter Evaluation of Cystatin C Measurement after Assay Standardization.
Bargnoux, Anne-Sophie; Pieroni, Laurence; Cristol, Jean-Paul et al

in Clinical Chemistry (2017), 63(4), 833-841

BACKGROUND: Since 2010, a certified reference material ERM-DA471/IFCC has been available for cystatin C (CysC). This study aimed to assess the sources of uncertainty in results for clinical samples ... [more ▼]

BACKGROUND: Since 2010, a certified reference material ERM-DA471/IFCC has been available for cystatin C (CysC). This study aimed to assess the sources of uncertainty in results for clinical samples measured using standardized assays. METHODS: This evaluation was performed in 2015 and involved 7 clinical laboratories located in France and Belgium. CysC was measured in a panel of 4 serum pools using 8 automated assays and a candidate isotope dilution mass spectrometry reference measurement procedure. Sources of uncertainty (imprecision and bias) were evaluated to calculate the relative expanded combined uncertainty for each CysC assay. Uncertainty was judged against the performance specifications derived from the biological variation model. RESULTS: Only Siemens reagents on the Siemens systems and, to a lesser extent, DiaSys reagents on the Cobas system, provided results that met the minimum performance criterion calculated according to the intraindividual and interindividual biological variations. Although the imprecision was acceptable for almost all assays, an increase in the bias with concentration was observed for Gentian reagents, and unacceptably high biases were observed for Abbott and Roche reagents on their own systems. CONCLUSIONS: This comprehensive picture of the market situation since the release of ERM-DA471/IFCC shows that bias remains the major component of the combined uncertainty because of possible problems associated with the implementation of traceability. Although some manufacturers have clearly improved their calibration protocols relative to ERM-DA471, most of them failed to meet the criteria for acceptable CysC measurements. [less ▲]

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See detailSerum Creatinine: Not So Simple!
DELANAYE, Pierre ULiege; Cavalier, Etienne ULiege; Pottel, Hans

in Nephron (2017), 136(4), 302-308

Measuring serum creatinine is cheap and commonly done in daily practice. However, interpretation of serum creatinine results is not always easy. In this review, we will briefly remind the physiological ... [more ▼]

Measuring serum creatinine is cheap and commonly done in daily practice. However, interpretation of serum creatinine results is not always easy. In this review, we will briefly remind the physiological limitations of serum creatinine due notably to its tubular secretion and the influence of muscular mass or protein intake on its concentration. We mainly focus on the analytical limitations of serum creatinine, insisting on important concept such as reference intervals, standardization (and IDMS traceability), analytical interferences, analytical coefficient of variation (CV), biological CV and critical difference. Because the relationship between serum creatinine and glomerular filtration rate is hyperbolic, all these CVs will impact not only the precision of serum creatinine but still more the precision of different creatinine-based equations, especially in low or normal-low creatinine levels (or high or normal-high glomerular filtration rate range). [less ▲]

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See detailBiomarkers Predicting Bone Turnover in the Setting of CKD.
Evenepoel, Pieter; Cavalier, Etienne ULiege; D'Haese, Patrick C.

in Current Osteoporosis Reports (2017)

PURPOSE OF THE REVIEW: Impaired bone quality contributes to the increased fracture risk in chronic kidney disease patients. Both low and high turnover bone disease may compromise bone quality. The ... [more ▼]

PURPOSE OF THE REVIEW: Impaired bone quality contributes to the increased fracture risk in chronic kidney disease patients. Both low and high turnover bone disease may compromise bone quality. The question arises whether bone biomarkers may be additive or replace bone histormorphometry for diagnosing the extremes of bone turnover. RECENT FINDINGS: Studies exploring the performance of established and emerging bone biomarkers against histomorphometric assessment of bone turnover are limited and overall yield inconclusive results as to their diagnostic utility. Bone biomarkers, although promising, currently fail to meet the needed diagnostic accuracy to replace bone histomorphometry and thus are not yet ready for clinical use. Bone biomarkers have not only several advantages, but also important limitations such as high biological variability, retention with kidney disease, preanalytical issues, and interassay variability. These important issues must be considered when developing and evaluating bone biomarkers. There is an urgent need for harmonization and standardization of available assays and additional bone biopsy studies. [less ▲]

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See detailDistinction between urine crystals by automated urine analyzer SediMAX conTRUST is specific but lacks of sensitivity.
Castiglione, Vincent ULiege; CAVALIER, Etienne ULiege; Diop, Coumba Souley ULiege et al

in Clinical Chemistry & Laboratory Medicine (2017)

Urine crystals are commonly encountered but few papers have been published about it while it is useful for patient’s follow-up, particularly the stone-formers. For the first time to our knowledge, we have ... [more ▼]

Urine crystals are commonly encountered but few papers have been published about it while it is useful for patient’s follow-up, particularly the stone-formers. For the first time to our knowledge, we have assessed the performance of an automated urine analyzer to identify and distinguish several types of crystals in a very large cohort of samples. We share in this report the results of method comparison of the SediMAX conTRUST with the polarized light microscopy which is the gold standard. We showed that the distinction between crystals and other urine elements was accurate. Negative predictive value was very good, but the positive predictive value was poor for most of crystals. The discrimination of different types of crystals between them by the automated urine analyzer still requires improvement to decrease reviewing rate and help clinician. [less ▲]

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See detailVitamin K plasma levels determination in human health
Fusaro, M; Gallieni, M; Rizzo, MA et al

in Clinical Chemistry & Laboratory Medicine (2017), 55(6), 789-799

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See detailWhen obtaining a blood sample from the right arm was not the right thing to do: a case of elevated parathyroid hormone levels 27 years after thyroidectomy.
Wolff, Fleur; Gervy, Christine; Cavalier, Etienne ULiege et al

in Clinical Chemistry & Laboratory Medicine (2016)

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See detailAssociation entre taux circulants de matrix-gla protéine et rigidité artérielle en transplantation rénale.
Dinic, Miriana; Maillard, Nicolas; DELANAYE, Pierre ULiege et al

Conference (2016, December)

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See detailPrevalence of the hypovitaminosis D among Moroccan woman consulting in ambulatory medicine
Nejjar, Basma ULiege; Bour, Abdellatif; Beaudart, Charlotte ULiege et al

in Journal of Innovative Research and Applied Sciences (2016), 3(2), 476-483

Actuellement, la vitamine D est considérée comme une hormone aux effets pleïotropes et ne peut plus être limitée uniquement qu’à la prévention du rachitisme et de l’ostéomalacie. Une déficience en ... [more ▼]

Actuellement, la vitamine D est considérée comme une hormone aux effets pleïotropes et ne peut plus être limitée uniquement qu’à la prévention du rachitisme et de l’ostéomalacie. Une déficience en vitamine D dans l’organisme est corollaire à une panoplie de pathologies osseuses et extra osseuses chroniques. Objectif : La présente étude s'intéressait à la prévalence de l'hypovitaminose D chez les femmes en médecine ambulatoire dans la région de Meknès au Maroc. Matériel et méthodes : Un échantillonnage de convenance de 1247 femmes pour lesquelles un clinicien avait prescrit le dosage de la 25 Hydroxyvitamine D(25OHD) était sélectionné entre Janvier 2013 et Décembre 2015 auprès de deux laboratoires. La concentration de la 25OHD, la saison de son dosage et l'âge ont été recensés. La calcémie n’était évaluée que pour 759 femmes de l'échantillon. Résultats : La prévalence de l'hypovitaminose D, pour un seuil inférieur à 20 ng/ml, était de 70,1%, et variait significativement en fonction de la saison et de l'âge. Les prescriptions du dosage de la 25OHD émanaient à 52.8% de médecins généralistes et de rhumatologues et 32,8% des dosages étaient enregistrés en hiver. La calcémie était de 2,28 mg/l (ET 0,22) chez les 759 (60,8%) femmes, 188 (37,8%) parmi elles, présentaient une hypocalcémie et une hypovitaminose D. La régression logistique indiquait que la saison (Exp (B)=0,23, p<0.01) était un facteur associé à la variation de l'hypovitaminose D. Conclusion : L’hypovitaminose D représente Mots un problème de santé publique et les médecines doivent être sensibilisés à ce sujet. [less ▲]

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See detailMyoferlin regulates cellular lipid metabolism and promotes metastases in triple-negative breast cancer
Blomme, Arnaud; Costanza, Brunella ULiege; De Tullio, Pascal ULiege et al

in Oncogene (2016)

Myoferlin is a multiple C2-domain-containing protein that regulates membrane repair, tyrosine kinase receptor function and endocytosis in myoblasts and endothelial cells. Recently it has been reported as ... [more ▼]

Myoferlin is a multiple C2-domain-containing protein that regulates membrane repair, tyrosine kinase receptor function and endocytosis in myoblasts and endothelial cells. Recently it has been reported as overexpressed in several cancers and shown to contribute to proliferation, migration and invasion of cancer cells. We have previously demonstrated that myoferlin regulates epidermal growth factor receptor activity in breast cancer. In the current study, we report a consistent overexpression of myoferlin in triple-negative breast cancer cells (TNBC) over cells originating from other breast cancer subtypes. Using a combination of proteomics, metabolomics and electron microscopy, we demonstrate that myoferlin depletion results in marked alteration of endosomal system and metabolism. Mechanistically, myoferlin depletion caused impaired vesicle traffic that led to a misbalance of saturated/unsaturated fatty acids. This provoked mitochondrial dysfunction in TNBC cells. As a consequence of the major metabolic stress, TNBC cells rapidly triggered AMP activated protein kinase-mediated metabolic reprogramming to glycolysis. This reduced their ability to balance between oxidative phosphorylation and glycolysis, rendering TNBC cells metabolically inflexible, and more sensitive to metabolic drug targeting in vitro. In line with this, our in vivo findings demonstrated a significantly reduced capacity of myoferlin-deficient TNBC cells to metastasise to lungs. The significance of this observation was further supported by clinical data, showing that TNBC patients whose tumors overexpress myoferlin have worst distant metastasis-free and overall survivals. This novel insight into myoferlin function establishes an important link between vesicle traffic, cancer metabolism and progression, offering new diagnostic and therapeutic concepts to develop treatments for TNBC patients. [less ▲]

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See detailUnexpected high levels of Cobalamin (Vitamin B12): Numerous interferences are decreased by PEG treatment.
LADANG, Aurélie ULiege; VRANKEN, Laura ULiege; CAVALIER, Etienne ULiege

Poster (2016, October)

Cobalamin (Vitamin B12) deficiency is a common cause of anemia. High levels of serum cobalamin is mainly due to cobalamin supplementation. It has also been associated with hematological malignancies ... [more ▼]

Cobalamin (Vitamin B12) deficiency is a common cause of anemia. High levels of serum cobalamin is mainly due to cobalamin supplementation. It has also been associated with hematological malignancies, liver or renal diseases. Cobalamin is sometimes found elevated in autoimmune disorders and infectious diseases but concerns are raised on the fact that those serum increases might be due to interference with immunoassays. Those data’s shed the lights on the high prevalence of interference in patients with unexpected high cobalamin level. Precipitation with PEG appears to be an easy and costless method to increase the reliability of cobalamin dosage. In accordance with our results, we recommand to treat systematically every serum with a cobalamin >1500 ng/L. [less ▲]

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See detailIohexol plasma clearance for measuring glomerular filtration rate in clinical practice and research: a review. Part 1 : How to measure glomerular filtration rate with iohexol?
DELANAYE, Pierre ULiege; Ebert, Natalie; Melsom, Toralf et al

in Clinical Kidney Journal (2016)

While there is general agreement on the necessity tomeasure glomerular filtration rate (GFR) inmany clinical situations, there is less agreement on the bestmethod to achieve this purpose. As the gold ... [more ▼]

While there is general agreement on the necessity tomeasure glomerular filtration rate (GFR) inmany clinical situations, there is less agreement on the bestmethod to achieve this purpose. As the gold standardmethod for GFR determination, urinary (or renal) clearance of inulin, fades into the background due to inconvenience and high cost, a diversity of filtrationmarkers and protocols compete to replace it. In this review, we suggest that iohexol, a non-ionic contrast agent, is most suited to replace inulin as the marker of choice for GFR determination. Iohexol comes very close to fulfilling all requirements for an ideal GFRmarker in terms of low extra-renal excretion, low protein binding and in being neither secreted nor reabsorbed by the kidney. In addition, iohexol is virtually non-toxic and carries a low cost. As iohexol is stable in plasma, administration and sample analysis can be separated in both space and time, allowing access to GFR determination across different settings. An external proficiency programme operated by Equalis AB, Sweden, exists for iohexol, facilitating interlaboratory comparison of results. Plasma clearance measurement is the protocol of choice as it combines a reliable GFR determination with convenience for the patient. Single-sample protocols dominate, butmultiple-sample protocolsmay bemore accurate in specific situations. In lowGFRs one ormore late samples should be included to improve accuracy. In patients with large oedema or ascites, urinary clearance protocols should be employed. In conclusion, plasma clearance of iohexol may well be the best candidate for a common GFR determination method. [less ▲]

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See detailIohexol plasma clearance for measuring glomerular filtration rate in clinical practice and research: a review. Part 2: Why to measure glomerular filtration rate with iohexol?
DELANAYE, Pierre ULiege; Melson, Toralf; Ebert, Natalie et al

in Clinical Kidney Journal (2016)

2 | P. Delanaye et al. Abstract A reliable assessment of glomerular filtration rate (GFR) is of paramount importance in clinical practice as well as epidemiological and clinical research settings. It is ... [more ▼]

2 | P. Delanaye et al. Abstract A reliable assessment of glomerular filtration rate (GFR) is of paramount importance in clinical practice as well as epidemiological and clinical research settings. It is recommended by Kidney Disease: Improving Global Outcomes guidelines in specific populations (anorectic, cirrhotic, obese, renal and non-renal transplant patients) where estimation equations are unreliable. Measured GFR is the only valuable test to confirm or confute the status of chronic kidney disease (CKD), to evaluate the slope of renal function decay over time, to assess the suitability of living kidney donors and for dosing of potentially toxic medication with a narrowtherapeutic index. Abnormally elevatedGFRor hyperfiltration in patients with diabetes or obesity can be correctly diagnosed only by measuring GFR. GFR measurement contributes to assessing the true CKD prevalence rate, avoiding discrepancies duetoGFRestimation with different equations. Usingmeasured GFR, successfullyaccomplished in large epidemiological studies, is the onlyway to study the potential link between decreased renal functionand cardiovascular or total mortality, being sure that this association is not due to confounders, i.e. non-GFR determinants of biomarkers. In clinical research, it has been shown that measured GFR (or measured GFR slope) as a secondary endpoint as compared with estimated GFR detected subtle treatment effects and obtained these results with a comparatively smaller sample size than trials choosing estimated GFR. Measuring GFR by iohexol has several advantages: simplicity, low cost, stability and low interlaboratory variation. Iohexol plasma clearance represents the best chance for implementing a standardized GFR measurement protocol applicable worldwide both in clinical practice and in research. [less ▲]

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See detailSarcopenia in daily practice: assessment and management
Beaudart, Charlotte ULiege; McCloskey, Eugène; Bruyère, Olivier ULiege et al

in BMC Geriatrics (2016), 16(170),

Background: Sarcopenia is increasingly recognized as a correlate of ageing and is associated with increased likelihood of adverse outcomes including falls, fractures, frailty and mortality. Several tools ... [more ▼]

Background: Sarcopenia is increasingly recognized as a correlate of ageing and is associated with increased likelihood of adverse outcomes including falls, fractures, frailty and mortality. Several tools have been recommended to assess muscle mass, muscle strength and physical performance in clinical trials. Whilst these tools have proven to be accurate and reliable in investigational settings, many are not easily applied to daily practice. Methods: This paper is based on literature reviews performed by members of the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) working group on frailty and sarcopenia. Face-to-face meetings were afterwards organized for the whole group to make amendments and discuss further recommendations. Results: This paper proposes some user-friendly and inexpensive methods that can be used to assess sarcopenia in real-life settings. Healthcare providers, particularly in primary care, should consider an assessment of sarcopenia in individuals at increased risk; suggested tools for assessing risk include the Red Flag Method, the SARC-F questionnaire, the SMI method or different prediction equations. Management of sarcopenia should primarily be patient centered and involve the combination of both resistance and endurance based activity programmes with or without dietary interventions. Development of a number of pharmacological interventions is also in progress. Conclusions: Assessment of sarcopenia in individuals with risk factors, symptoms and/or conditions exposing them to the risk of disability will become particularly important in the near future. [less ▲]

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See detailQuantification of serum androstanediol glucuronide
LE GOFF, Caroline ULiege; Fabregat Cabello, Neus ULiege; Cavalier, Etienne ULiege

Scientific conference (2016, September 14)

Validation of the method by LCMSMS

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See detailDevelopment of a new methodology for the simultaneous determination of cholecalciferol and 24,25(OH)2D3 by LC-MS/MS
Fabregat Cabello, Neus ULiege; Huyghebaert, Loreen ULiege; Gonzalez Antuña, Ana et al

Scientific conference (2016, September)

In the present work an ultra-high-pressure liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for the simultaneous determination of cholecalciferol and 24,25(OH)2D3 in serum has been ... [more ▼]

In the present work an ultra-high-pressure liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for the simultaneous determination of cholecalciferol and 24,25(OH)2D3 in serum has been developed and validated. The validation was carried out by analyzing serum fortified at five concentration levels. In all cases, recoveries ranging from 89-109% for cholecalciferol and 85-111% for 24,25(OH)2D3 and CV below 7% were attained with this approach. As a consequence, this procedure is ready to assess the rates of synthesis of cholecalciferol and 24,25(OH)2D3 in the skin either by sun exposition or by oral absorption. [less ▲]

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