Etude analytique des trois trousses de cystatine C et impact sur les formules basées sur la cystatine pour l'estimation du DFG.
Cavalier, Etienne ; ; et al
in Néphrologie & Thérapeutique (2008, November), 4(6), 399-400Detailed reference viewed: 42 (8 ULg)
Vitamine D: effets « phosphocalciques » et « non-phosphocalciques ».
Cavalier, Etienne ;
Conference (2008, October)Detailed reference viewed: 30 (2 ULg)
Bioformation ACORATA: Exploration biologique des pathologies osseuses
Conference given outside the academic context (2008)Detailed reference viewed: 18 (4 ULg)
Circulating Concentrations of 25-Hydroxyvitamin D after a Single Oral Dose of 100.000 IU of Vitamin D2 or Vitamin D3
Cavalier, Etienne ; ; et al
in Journal of Bone and Mineral Research (2008, September), 23Detailed reference viewed: 28 (7 ULg)
Analytical Validation of Diasorin Liaison and Roche Elecsys Methods for the Determination of Osteocalcin
Cavalier, Etienne ; Delanaye, Pierre ; Carlisi, Ignazia et al
in Journal of Bone and Mineral Research (2008, September), 23Detailed reference viewed: 188 (9 ULg)
Measurements of Vitamin D Do Not Necessarily Reflect What You Give to Your Patients
Cavalier, Etienne ; ;
in Journal of Bone and Mineral Research (2008, September), 23Detailed reference viewed: 30 (3 ULg)
Qualité optimale d’un prélèvement: Mieux connaître les facteurs pouvant modifier la qualité d’un résultat
Conference (2008, May 31)Detailed reference viewed: 17 (5 ULg)
Critical difference: from results to interpretation and its impact on patient care
Gilmaire, Julie ; Descy, Julie ; Haleng, Jeanine et al
Poster (2008, May)Detailed reference viewed: 34 (7 ULg)
False positive PTH results: An easy strategy to test and detect analytical interferences in routine practice
Cavalier, Etienne ; ; Chapelle, Jean-Paul et al
in Clinica Chimica Acta (2008), 387(1-2), 150-152
Background: As other immunoassays, PTH determination is not free from interferences. Indeed, natural antibodies like heterophile antibodies (HAMA) and rheumatoid factor (RF) can induce falsely elevated ... [more ▼]
Background: As other immunoassays, PTH determination is not free from interferences. Indeed, natural antibodies like heterophile antibodies (HAMA) and rheumatoid factor (RF) can induce falsely elevated results, leading to misdiagnosis and expensive unnecessary explorations. However, in routine practice, these interferences are not always obvious to detect. Methods: On 2084 PTH samples, we applied a validation strategy in four steps to screen for HAMA and rheumatoid factor interferences. Results: 36% of our samples presented an elevated PTH. We found a clinically plausible reason for 91% of them. The remaining 63 suspicious samples were treated with HBT and 40% of them were found to be HAMA positive. RF determination was performed on the HAMA-negative samples and RE was positive in 21 of them. They were then treated with RF-Absorbent. Nine of these 21 samples presented RE interference. Conclusion: Applying this strategy in our routine validation, we managed to avoid spuriously elevated PTH results, which could have caused medical errors as well as unnecessary cost-effective extra-investigations. (c) 2007 Elsevier B.V. All rights reserved. [less ▲]Detailed reference viewed: 62 (6 ULg)
Prévalence de la maladie rénale chronique par l’estimation du DFG avec une formule basée sur la créatinine versus la cystatine C
Delanaye, Pierre ; Cavalier, Etienne ; Saint-Remy, Annie et al
in Néphrologie & Thérapeutique (2008), 4(6), 004Detailed reference viewed: 22 (7 ULg)
Impact de la stabilité de la cystatine C sur la validité des mesures dans les études rétrospectives
; Delanaye, Pierre ; et al
in Néphrologie & Thérapeutique (2008), 4(6), 052Detailed reference viewed: 41 (7 ULg)
Analytical quality of calcitonin determination and its effect on the adequacy of screening for medullary carcinoma of the thyroid.
Cavalier, Etienne ; Carlisi, Ignazia ; Chapelle, Jean-Paul et al
in Clinical Chemistry (2008), 54(5), 929-30Detailed reference viewed: 21 (4 ULg)
Analytical study of three cystatin C assays and their impact on cystatin C-based GFR-prediction equations.
Delanaye, Pierre ; ; et al
in Clinica Chimica Acta (2008), 398(1-2), 118-24
BACKGROUND: Cystatin C-based equations are used to estimate GFR. However, three cystatin C immunoassays are on the market. Difference in cystatin C assays could have strong consequences on the accuracy ... [more ▼]
BACKGROUND: Cystatin C-based equations are used to estimate GFR. However, three cystatin C immunoassays are on the market. Difference in cystatin C assays could have strong consequences on the accuracy and precision of cystatin C-based equations. We have performed an analytical study of these three assays and studied potential differences between assays on the precision of cystatin C-based equations. METHODS: We have studied imprecision, recovery, linearity and interferences of the three immunoassays (nephelometric assay from Siemens and turbidimetric assays from Dako and Gentian). The impact of differences in cystatin C assays has been studied for the equations published by Levey (Siemens assay) and Grubb (Dako assay). RESULTS: Analytical performance of the Dako assay is slightly less high. For cystatin C values below 2.5 mg/L, no statistical difference is found between results given by the Dako and the Gentian assays. So, both assays can be used in the Grubb equation. Cystatin C results are different with the Siemens assay. The Levey equation, built with the Siemens assay, can only be used with cystatin C values measured with this assay. Using the Dako or Gentian assay results in the Levey equation can lead to differences in estimating GFR up to 6 mL/min/1.73 m2. Differences can reach 9.5 mL/min/1.73 m2 if the Siemens assay is used in the Grubb equation. CONCLUSION: The Siemens and Gentian assays seem analytically more valid than the Dako assay for cystatin C determination. Differences in cystatin C assays can lead to significant differences in cystatin C-based equations. However, these differences seem less important than the differences observed with creatinine and creatinine-based equations. [less ▲]Detailed reference viewed: 34 (10 ULg)
New data on the intraindividual variation of cystatin C.
Delanaye, Pierre ; Cavalier, Etienne ; Depas, Gisèle et al
in Nephron. Clinical Practice (2008), 108(4), 246-8
BACKGROUND: Cystatin C is a new interesting marker of glomerular filtration rate (GFR). However, data regarding its biological variance are scarce and conflicting. The ability of cystatin C to ... [more ▼]
BACKGROUND: Cystatin C is a new interesting marker of glomerular filtration rate (GFR). However, data regarding its biological variance are scarce and conflicting. The ability of cystatin C to longitudinally follow renal function in patients therefore remains questionable. METHODS: 12 healthy subjects (6 men and 6 women) were included in the final statistical analysis. Serum creatinine, plasma cystatin C and GFR were measured twice after a 1-week interval on the same day, at the same time, and under the same preanalytical and analytical conditions. GFR was measured with an iohexol method. Serum creatinine was measured with a compensated Jaffe and an enzymatic method. Plasma cystatin C was measured by a particle-enhanced immunonephelometric method. Analytical (CV(A)) and within-subject (CV(I)) variances were classically calculated. RESULTS: CV(A) for creatinine (Jaffe and enzymatic methods) and cystatin C was 2.5, 0.97 and 1.29%, respectively. CV(I) was 5.8, 5 and 4.5% for the Jaffe creatinine, enzymatic creatinine and cystatin C determinations, respectively. CONCLUSION: Our study confirms that intraindividual variation of cystatin C and creatinine are similar. Therefore, from a biological point of view, cystatin C seems as accurate as creatinine for the longitudinal follow-up of renal function in daily clinical practice. [less ▲]Detailed reference viewed: 37 (5 ULg)
Cystatin C or Creatinine for Detection of Stage 3 Chronic Kidney Disease in Anorexia Nervosa.
Delanaye, Pierre ; Cavalier, Etienne ; Radermecker, Régis et al
in Nephron. Clinical Practice (2008), 110(3), 158-163
Background: Patients with anorexia nervosa (AN) are at a high risk of renal failure. Chronic kidney disease (CKD) is often missed in these patients because the serum creatinine is a poor marker of kidney ... [more ▼]
Background: Patients with anorexia nervosa (AN) are at a high risk of renal failure. Chronic kidney disease (CKD) is often missed in these patients because the serum creatinine is a poor marker of kidney function. We studied the utility of cystatin C to detect renal failure in this population. Method: Twenty-seven AN patients were studied. Glomerular filtration rates (GFR) were measured with the chromium-51- ethylenediaminetetraacetate ((51)Cr-EDTA) method. We compared the ability of creatinine and cystatin C to detect stage 3 CKD (GFR below 60 ml/min) by ROC curve analysis. Results: In this cohort, there is no correlation between GFR and serum creatinine, but there is a significant correlation between cystatin C and GFR. By ROC analysis, the cystatin C concentration is better than the serum creatinine concentration for the detection of stage 3 CKD (area under the curve of 0.86 vs. 0.61, p = 0.05). Conclusion: Plasma cystatin C is better than serum creatinine in detecting stage 3 CKD in patients with AN. [less ▲]Detailed reference viewed: 51 (5 ULg)
Can laboratory tests help to define the profile of desensitised patients or patients at high risk of severe reaction ?
Gadisseur, Romy ; ; Cataldo, Didier et al
in Allergy (2008), 63(Suppl. 88), 1773Detailed reference viewed: 50 (21 ULg)
Creatinine calibration in NHANES: is a revised MDRD study formula needed?
Delanaye, Pierre ; Cavalier, Etienne ; et al
in American Journal of Kidney Diseases (2008), 51(4), 709709-10Detailed reference viewed: 29 (2 ULg)
Cystatin C-based equations: don't repeat the same errors with analytical considerations.
Delanaye, Pierre ; Cavalier, Etienne ; Krzesinski, Jean-Marie et al
in Nephrology Dialysis Transplantation (2008), 23(3), 10651065-6Detailed reference viewed: 13 (2 ULg)