Flavonoids inhibit malignant tumor invasion in vitro.

in Progress in Clinical & Biological Research (1988), 280

Perfusion of Human Umbilical Veins. A New Approach to Study the Interactions of Circulating Malignant Cells with Vascular Wall and Their Modulations

in Invasion & Metastasis (1988), 8(6), 332-50

Interactions of malignant or non-malignant human and rodent cells with the vascular wall were studied using perfused human umbilical cord veins. The integrity of perfused endothelium was confirmed by ... [more ▼]

Interactions of malignant or non-malignant human and rodent cells with the vascular wall were studied using perfused human umbilical cord veins. The integrity of perfused endothelium was confirmed by morphological and functional criteria. Highly malignant cells in vivo adhered to the endothelial cells, as shown by scanning electron microscopy. The specific attachment of radiolabelled malignant cells to the whole vein was already maximal within 30-60 min and remained stable for perfusion flow rates ranging between 10 and 60 ml/min. It increased proportionally to the number of cells infused and could be modulated by human platelets, human fibronectin and rabbit anti-laminin antibodies. In contrast, the binding of human or rodent non-malignant cells in vivo, of human red blood cells and of human platelets to the endothelial cells was negligible under similar experimental conditions. This perfusion system therefore represents a new model for elucidating some mechanisms involved in tumour cell arrest in vivo. [less ▲]

The anti-invasive flavonoid (+)-catechin binds to laminin and abrogates the effect of laminin on cell morphology and adhesion

in Experimental Cell Research (1987), 173(1), 193-205

To study the effect of the flavonoid (+)-catechin on cell-matrix interactions two cell types with a different morphology on and adhesion to laminin were used. MO4 virally transformed fetal mouse cells ... [more ▼]

To study the effect of the flavonoid (+)-catechin on cell-matrix interactions two cell types with a different morphology on and adhesion to laminin were used. MO4 virally transformed fetal mouse cells adhere and spread when cultured on top of laminin-coated coverslips or on human amnion basement membrane. M5076 mouse reticulum cell sarcoma cells poorly adhere to these substrates and remain round. Both cell types are invasive in confronting cultures with embryonic chick heart fragments. (+)-Catechin binds to laminin in a pH-dependent way. Pretreatment of laminin-coated coverslips or amnion basement membrane with 0.5 mM (+)-catechin abrogates the effect of laminin on cell morphology and adhesion. MO4 cells do not adhere to the pretreated substrates and remain round, while M5076 cells now adhere and spread. (+)-Catechin inhibits the invasion of MO4 cells but not of M5076 cells into embryonic chick heart in vitro. We speculate that the anti-invasive activity of the flavonoid to MO4 cells is the result of its interference with MO4 cell adhesion to laminin. Invasion of M5076 cells does not imply adhesion to and spreading on laminin. [less ▲]

Interactions between fibroblasts and a reconstituted basement membrane matrix

in Journal of Investigative Dermatology (1987), 89

A gel-like reconstituted basement membrane matrix containing type IV collagen, laminin, entactin, nidogen, and heparan sulfate proteoglycan was used to examine the interactions between normal calf skin ... [more ▼]

A gel-like reconstituted basement membrane matrix containing type IV collagen, laminin, entactin, nidogen, and heparan sulfate proteoglycan was used to examine the interactions between normal calf skin fibroblasts and basement membranes. Within 6 h after seeding, fibroblasts initiated a migration that resulted in the formation of a cellular network after 1 day of culture on top of the gel. Electron microscopy revealed that fibroblasts were able to remodel the basement membrane matrix by penetrating into the gel (from day 3), depositing fibronectin and collagen fibers, and retracting this extracellular matrix. Fibroblasts cultured on the Engelbreth-Holm-Swarm reconstituted basement membrane matrix displayed ultrastructural features characterized by a poor synthetic apparatus (rough endoplasmic reticulum and Golgi vesicles), a large cytoskeleton, and intracytoplasmic vesicles containing laminin. Thus the reconstituted basement membrane matrix is remodeled by skin fibroblasts, and reciprocally their ultrastructural morphologic features are affected by this matrix. [less ▲]

Flavonoids : tools for the study of tumor invasion in vitro.

in Plant Flavonoids in Biology and Medicine: Biochemical, Pharmacological and Structure-Activity Relationships. (1986)

Interaction between human breast adenocarcinoma cells and type I collagen matrix requires fibronectin.

in Biology and Chemistry of Basement Membranes. (1985)

An accumulation of laminin precedes invasion by transformed MO4 cells.

in Biology and Chemistry of Basement Membranes. (1985)

Alteration of circadian variation in serum concentrations of carcino-embryonic antigen (CEA) in cancer.

in Protides of the Biological Fluids. (1984)

Infections respiratoires et pathologie de la clairance muco-ciliaire

in Revue Médicale de Liège (1981), 36(20), 760-6

L'ultrastructure et le fonctionnement des cils de la muqueuse respiratoire sont actuellement mieux connus. Il est donc possible de diagnostiquer les dyskinésies ciliaires qui peuvent expliquer l ... [more ▼]

L'ultrastructure et le fonctionnement des cils de la muqueuse respiratoire sont actuellement mieux connus. Il est donc possible de diagnostiquer les dyskinésies ciliaires qui peuvent expliquer l'inefficacité de "l'escalator mucociliaire". [less ▲]