References of "Cambier, Carole"
     in
Bookmark and Share    
Peer Reviewed
See detailComparison of bronchodilators in feline airways: In vitro and in vivo investigations
Leemans, Jérôme ULg; Kirschvink, Nathalie; Delvaux, F. et al

in In proceedings: 15th Congress European College of Veterinary Internal Medicine – companion animals (2005)

Detailed reference viewed: 13 (3 ULg)
Peer Reviewed
See detailEvaluation of the bronchoprotective effects of inhaled salmeterol, fenoterol and oxitropium in healthy cats
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, F. et al

in Fundamental & Clinical Pharmacology (2005), 19

Detailed reference viewed: 12 (1 ULg)
Peer Reviewed
See detailIn vitro and in vivo effects of salbutamol acetonide on cat airways
Leemans, Jérôme ULg; Kirschvink, Nathalie; Delvaux, F. et al

in Proceedings: Société Belge de Physiologie et de Pharmacologie Fondamentales et Cliniques (2005)

Detailed reference viewed: 17 (3 ULg)
Peer Reviewed
See detailIn vitro pharmacological activity of salbutamol acetonide on the isolated guinea-pig trachea and porcine bronchus
Leemans, Jérôme ULg; Kirschvink, Nathalie; Delvaux, F. et al

in Fundamental & Clinical Pharmacology (2005), 19

Detailed reference viewed: 18 (6 ULg)
Full Text
Peer Reviewed
See detailEvaluation expérimentale de la fonction pulmonaire chez le porc
Halloy, D.; Cambier, Carole ULg; Kirschvink, N. et al

in Annales de Médecine Vétérinaire (2004), (148), 91-96

This synthesis aims to review the pulmonary function tests available in swine. Two techniques are used in order to measure the variations of the mechanical properties of the respiratory system. The ... [more ▼]

This synthesis aims to review the pulmonary function tests available in swine. Two techniques are used in order to measure the variations of the mechanical properties of the respiratory system. The oesophageal balloon remains the most used technique in order to determine these properties in pigs. However, it requires a systematic anaesthesia of the animal. The impulse oscillometry specifically measures the mechanical properties of the respiratory system, by a non invasive way, but the animals have to be trained to be immobilized or sedated to perform the measurement. The whole body arometric plethysmography allows measuring the respiratory pattern in unsedated freely moving piglets. That method allows investigating pigs for long term studies while minimizing the stress related to handling. From this point of view, it represents the less stressing technique for pigs. Finally, blood gases analysis is the easiest method to use in the field. It permits to assess respiratory function by measuring pH and blood partial pressures in oxygen and carbon dioxide [less ▲]

Detailed reference viewed: 41 (6 ULg)
Full Text
Peer Reviewed
See detailOxygen consumption and blood oxygen transport in endotoxemic calves
Cambier, Carole ULg; Clerbaux, T.; Detry, B. et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailInfluence of chloride and phosphate on the binding of oxygen to canine and feline red blood cells
Cambier, Carole ULg; Wiernickx; Clerbaux, Th et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailHaemoglobin oxygen affinity and regulating factors of the blood oxygen transport in canine and feline blood
Cambier, Carole ULg; Wierinckx, Maude ULg; Clerbaux, T. et al

in Research in Veterinary Science (2004), 77(1), 83-88

Complete dynamic oxygen equilibrium curves (OEC) on dogs and cats whole blood were measured at 33, 37 and 41 degrees C. OEC were also run at three partial carbon dioxide pressures (20, 40 and 80 mmHg) as ... [more ▼]

Complete dynamic oxygen equilibrium curves (OEC) on dogs and cats whole blood were measured at 33, 37 and 41 degrees C. OEC were also run at three partial carbon dioxide pressures (20, 40 and 80 mmHg) as well as at five pH levels (7.2, 7.3, 7.4, 7.5 and 7.6). 2,3- diphosphoglycerate (DPG) concentrations were determined. Results were compared to those previously published in humans, using the same experimental method [Comp. Biochem. Physiol. 106 (1993) 687]. In standard conditions (pH 7.4, pCO2 40 mmHg and temperature 37 degrees C), the partial oxygen pressure at half-saturation of haemoglobin (p50) was 30.0+/-1.3 mmHg for dogs and 34.1+/-1.8 mmHg for cats. Cat's OEC was thus rightshifted compared to dog's OEC, itself rightshifted compared to human OEC. 2,3-DPG concentrations were higher in dogs than in men until they were very low in cats. Contrary to that observed in human medicine, no significant correlation was identified between standard p50 and canine 2,3-DPG values. Influence of pH, pCO2 and temperature on the OEC was saturation dependent. In dogs, Delta log p50/Delta pH was equal to -0.370, Delta log p50/Delta log pCO2 was 0.093 and Delta log p50/Delta T was 0.020. In cats, Delta log p50/Delta pH was equal to -0.405, Delta log p50/Delta log pCO2 was 0.080 and Delta log p50/Delta T was 0.016. Practically, temperature and pH variations exert a lesser influence in domestic carnivores than in humans, effect of pCO2 being similar in both. [less ▲]

Detailed reference viewed: 38 (3 ULg)
Full Text
Peer Reviewed
See detailCalculation of bovine haemoglobin oxygen saturation by algorithms integrating age, haemoglobin content, blood pH, partial pressures of oxygen and carbon dioxide in the blood, and temperature.
Detry, Benoit; Cambier, Carole ULg; Frans, A. et al

in Veterinary Journal (2003), 165(3), 258-265

In human and veterinary medicine, arterial and venous haemoglobin oxygen saturations are often used to estimate the severity of a disease and to guide therapeutic decisions. In veterinary medicine ... [more ▼]

In human and veterinary medicine, arterial and venous haemoglobin oxygen saturations are often used to estimate the severity of a disease and to guide therapeutic decisions. In veterinary medicine, haemoglobin oxygen saturation (SO(2)) is usually calculated using a blood gas analyser and algorithms developed for humans. It is possible, therefore, that the values obtained in animals may be distorted, particularly in animals with a high haemoglobin oxygen affinity, like young calves. In order to verify this hypothesis, we compared the arterial (SaO(2)) and venous (SvO(2)) haemoglobin oxygen saturations calculated using three different algorithms, and the oxygen exchange fraction (OEF) at the tissue level, which is the degree of haemoglobin desaturation between arterial and venous blood (SaO(2)-SvO(2)), with the values obtained from the whole bovine oxygen equilibrium curve (OEC) determined by a reference method. The blood gas analysers underestimated SvO(2) values; consequently, the OEF was overestimated (by about 10%). Two methods of reducing these errors were assessed. As the haemoglobin oxygen affinity decreases during the first month of life in calves a relationship between PO(2) at 50% haemoglobin saturation (P50) and age was established in order to correct the calculated values of venous and arterial SO(2), taking into account the estimated position of the OEC. This method markedly reduced the error for SvO(2) and OEF. Secondly, the SO(2) was calculated using a mathematical model taking into account the age of the animal and the specific effects of pH, PCO(2), and temperature on the bovine OEC. Using this method, the mean difference between the OEF values calculated using the mathematical model and those calculated by the reference method was close to zero. The errors produced by blood gas analysers can thus be minimised in two ways: firstly, by simply introducing a P50 estimated from the age of the calf into the analyser before the measurement; and secondly, by calculating the SO(2) using a mathematical model applied to the bovine OEC. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Full Text
Peer Reviewed
See detailMechanisms controlling the oxygen consumption in experimentally induced hypochloremic alkalosis in calves
Cambier, Carole ULg; Clerbaux, Thierry; Amory, Hélène ULg et al

in Veterinary Research (2002), 33

The study was carried out on healthy Friesian calves (n = 10) aged between 10 and 30 days. Hypochloremia and alkalosis were induced by intravenous administration of furosemide and isotonic sodium ... [more ▼]

The study was carried out on healthy Friesian calves (n = 10) aged between 10 and 30 days. Hypochloremia and alkalosis were induced by intravenous administration of furosemide and isotonic sodium bicarbonate. The venous and arterial blood samples were collected repeatedly. 2,3-diphosphoglycerate (2,3-DPG), hemoglobin and plasmatic chloride concentrations were determined. The red blood cell chloride concentration was also calculated. pH, PCO2 and PO2 were measured in arterial and mixed venous blood. The oxygen equilibrium curve (OEC) was measured in standard conditions. The correspondence of the OEC to the arterial and mixed venous compartments was calculated, taking blood temperature, pH and PCO2 values into account. The oxygen exchange fraction (OEF%), corresponding to the degree of blood desaturation between the arterial and mixed venous compartments and the amount of oxygen released at the tissue level by 100 mL of blood (OEF Vol%) were calculated from the arterial and mixed venous OEC, combined with PO2 and hemoglobin concentration. Oxygen delivery (DO2) was calculated using the arterial oxygen content, the cardiac output measured by thermodilution, and the body weight of the animal. The oxygen consumption (VO2) was derived from the cardiac output, OEF Vol% and body weight values. Despite the plasma hypochloremia, the erythrocyte chloride concentration was not influenced by furosemide and sodium bicarbonate infusion. Due to the alkalosis-induced increase in the 2,3-DPG, the standard OEC was shifted to the right, allowing oxygen to dissociate from hemoglobin more rapidly. These changes opposed the increased affinity of hemoglobin for oxygen induced by alkalosis. Moreover, respiratory acidosis, hemoconcentration, and the slight decrease in the partial oxygen pressure in mixed venous blood (Pvo(2)) tended to improve the OEF Vol% and maintain the oxygen consumption in a physiological range while the cardiac output, and the oxygen delivery were significantly decreased. It may be concluded that, despite reduced oxygen delivery, oxygen consumption is maintained during experimentally induced hypochloremic alkalosis in healthy 10-30 day old calves [less ▲]

Detailed reference viewed: 56 (14 ULg)
Full Text
Peer Reviewed
See detailBlood oxygen binding in hypoxaemic calves
Cambier, Carole ULg; Clerbaux; Detry, Bruno et al

in Veterinary Research (2002), 33(3), 283-290

Blood oxygen transport and tissue oxygenation were studied in 28 calves from the Belgian White and Blue breed (20 healthy and 8 hypoxaemic ones). Hypoxaemic calves were selected according to their high ... [more ▼]

Blood oxygen transport and tissue oxygenation were studied in 28 calves from the Belgian White and Blue breed (20 healthy and 8 hypoxaemic ones). Hypoxaemic calves were selected according to their high respiratory frequency and to their low partial oxygen pressure (PaO2) in the arterial blood. Venous and arterial blood samples were collected, and 2,3-diphosphoglycerate, adenosine triphosphate, chloride, inorganic phosphate and hemoglobin concentrations, and pH, PCO, and PO2 were determined. An oxygen equilibrium curve (OEC) was measured in standard conditions, for each animal. The arterial and venous OEC were calculated, taking body temperature, pH and PCO2 values in arterial and venous blood into account. The oxygen exchange fraction (OEF%), corresponding to the degree of blood desaturation between the arterial and the venous compartments, and the amount of oxygen released at the tissue level by 100 mL of blood (OEF Vol%) were calculated from the arterial and venous OEC combined with the PO2 and hemoglobin concentration. In hypoxaemic calves investigated in this study, the hemoglobin oxygen affinity, measured under standard conditions, was not modified. On the contrary, in vivo acidosis and hypercapnia induced a decrease in the hemoglobin oxygen affinity in arterial blood, which combined to the decrease in PaO2 led to a reduced hemoglobin saturation degree in the arterial compartment. However, this did not impair the oxygen exchange fraction (OEF%), since the hemoglobin saturation degree in venous blood was also diminished. [less ▲]

Detailed reference viewed: 29 (15 ULg)
Peer Reviewed
See detailDoppler echocardiographic measurement of cardiac output in the calf: a preliminary study
Amory, Hélène ULg; Sandersen, Charlotte ULg; Brihoum, M et al

in Proceedings of the World Assoc. Buiatrics Congress (2000)

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailBlood Oxygen Binding in Double-Muscled Calves and Dairy Calves with Conventional Muscle Conformation
Cambier, Carole ULg; Clerbaux, T.; Detry, B. et al

in American Journal of Veterinary Research (2000), 61(3), 299-304

OBJECTIVE: To assess in vivo blood oxygen binding in double-muscled calves and dairy calves with conventional muscle conformation. ANIMALS: 58 dairy and 48 double-muscled calves. PROCEDURE: Calves were ... [more ▼]

OBJECTIVE: To assess in vivo blood oxygen binding in double-muscled calves and dairy calves with conventional muscle conformation. ANIMALS: 58 dairy and 48 double-muscled calves. PROCEDURE: Calves were classified as neonatal (24 hours old) or older calves (2 to 26 days old). Venous and arterial blood samples were collected, and hemoglobin concentration, pH, PCO2, and PO2 were determined. Blood oxygen equilibrium curves (OEC) under standard conditions were constructed, and the oxygen exchange fraction (OEF) and the amount of oxygen released at the tissue level by 100 ml of blood (OEF Vol%) were calculated. RESULTS: In each breed, partial pressure of oxygen at 50% saturation of hemoglobin (P50) under standard conditions was significantly higher in older than in neonatal calves, indicating a right shift in OEC with age. Venous P50 was significantly lower in neonatal double-muscled calves than in neonatal dairy calves, but arterial and venous P50 were significantly higher in older double-muscled calves than in older dairy calves. In double-muscled, but not in dairy, calves, OEF was significantly higher in older than in neonatal calves. In neonatal calves, OEF Vol% was not significantly different between breeds, but OEF Vol% was significantly higher in older double-muscled calves than in older dairy calves. CONCLUSIONS AND CLINICAL RELEVANCE: The lower OEF in neonatal double-muscled calves, compared with dairy calves, could contribute to the higher sensitivity of double-muscled calves to hypoxia. Blood oxygen affinity decreased with age, but OEF and OEF Vol% were unchanged with age in dairy calves, whereas they increased with age in double-muscled calves [less ▲]

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailEffects of Hyperchloremia on Blood Oxygen Binding in Healthy Calves
Cambier, Carole ULg; Detry, B.; Beerens, Dominique ULg et al

in Journal of Applied Physiology (Bethesda, Md. : 1985) (1998), 85(4), 1267-1272

Three different levels of hyperchloremia were induced in healthy Friesian calves to study the effects of chloride on blood oxygen transport. By infusion, the calves received either 5 ml/kg of 0.9% NaCl ... [more ▼]

Three different levels of hyperchloremia were induced in healthy Friesian calves to study the effects of chloride on blood oxygen transport. By infusion, the calves received either 5 ml/kg of 0.9% NaCl (low-level hyperchloremia; group A), 5 ml/kg of 7.5% NaCl (moderate hyperchloremia; group B), or 7.5 ml/kg of 7.5% NaCl (high-level hyperchloremia; group C). Blood was sampled from the jugular vein and the brachial artery. Chloride concentration, hemoglobin content, arterial and venous pH, PCO2, and PO2 were determined. At each time point (0, 15, 30, 60, and 120 min), the whole blood oxygen equilibrium curve (OEC) was measured under standard conditions. In groups B and C, hyperchloremia was accompanied by a sustained rightward shift of the OEC, as indicated by the significant increase in the standard PO2 at 50% hemoglobin saturation. Infusion of hypertonic saline also induced relative acidosis. The arterial and venous OEC were calculated, with body temperature, pH, and PCO2 values in arterial and venous blood taken into account. The degree of blood desaturation between the arterial and the venous compartments [O2 exchange fraction (OEF%)] and the amount of oxygen released at tissue level by 100 ml of bovine blood (OEF vol%) were calculated from the arterial and venous OEC combined with the PO2 and hemoglobin concentration. The chloride-induced rightward shift of the OEC was reinforced by the relative acidosis, but the altered PO2 values combined with the lower hemoglobin concentration explained the absence of any significant difference in OEF (% and vol%). We conclude that infusion of hypertonic saline induces hyperchloremia and acidemia, which can explain the OEC rightward shift observed in arterial and peripheral venous blood. [less ▲]

Detailed reference viewed: 31 (4 ULg)
Peer Reviewed
See detailPathologie bovine : transport de l’oxygène et solutions salines
Gustin, Pascal ULg; Cambier, Carole ULg; Frans, A. et al

in Agricontact (1998), 302

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailInfluence of Age and Breed on the Binding of Oxygen to Red Blood Cells of Bovine Calves
Gustin, Pascal ULg; Detry, B.; Robert, Alain ULg et al

in Journal of Applied Physiology (Bethesda, Md. : 1985) (1997), 82(3), 784-90

The influence of somatic growth and genetic selection on the whole blood oxygen equilibrium curve (OEC) was measured under standard conditions in double-muscled and dairy calves during their first 3 mo of ... [more ▼]

The influence of somatic growth and genetic selection on the whole blood oxygen equilibrium curve (OEC) was measured under standard conditions in double-muscled and dairy calves during their first 3 mo of life. Crossbreed animals were also investigated. Hemoglobin, 2,3-diphosphoglycerate (DPG), Cl, and Pi concentrations were also measured. The percentage of fetal hemoglobin (HbF) was determined. The influence of exogenous Cl, Pi, and pH on the OEC was also assessed. The PO2 at 50% hemoglobin saturation (P50) increased during somatic growth, probably because of the increase in DPG recorded in double-muscled neonates and to the progressive disappearance of HbF in both breeds. The oxygen exchange fraction (OEF%) was used to assess the combined influence of the OEC shift and OEC shape changes on blood oxygen desaturation under standard conditions, when the PO2 decreases within a physiological range. The OEF% showed an increase during the first month, then a stabilization. The effects of Cl, Pi, and pH in Friesian calves were similar as in adult cattle. Double-muscled neonates had a lower P50, OEF% values, and DPG concentrations and higher hemoglobin and Cl concentrations than Friesian neonates. The Pi concentration and the percentage of HbF were similar in both breeds. The pH and the Cl concentration had significantly less effect on the OEC in double-muscled than in Friesian calves. Crossbreed animals exhibited intermediate parameter values, between those recorded for double-muscled and Friesian calves. All differences between breeds progressively disappeared during the first month. These data show that blood function changes markedly in calves during the first month of life and that genetic selection can alter blood function. [less ▲]

Detailed reference viewed: 32 (10 ULg)
Peer Reviewed
See detailA new acute respiratory distress syndrome in newborn calves : clinical, laboratory and post-mortem findings
Dardenne, A; Danlois, F; Rollin, Frédéric ULg et al

in In Proceedings of the 15th Comparative Respiratory Society Meeting (1997)

Detailed reference viewed: 23 (2 ULg)
Full Text
Peer Reviewed
See detailThe Effects of Hypertonic Saline in Healthy and Diseased Animals
Cambier, Carole ULg; Ratz, V.; Rollin, Frédéric ULg et al

in Veterinary Research Communications (1997), 21(5), 303-316

In this review, the pharmacological effects of administering hypertonic solutions to both healthy animals and during experimentally induced diseases are considered with a view to understanding the ... [more ▼]

In this review, the pharmacological effects of administering hypertonic solutions to both healthy animals and during experimentally induced diseases are considered with a view to understanding the mechanisms behind the possible clinical efficacy of such treatment. The review focuses successively on haemorrhagic shock, endotoxic shock and hypokalaemic metabolic alkalosis. How hypertonic saline solutions affect oxygen transport by haemoglobin is also considered. [less ▲]

Detailed reference viewed: 18 (1 ULg)