References of "CHARLIER, Corinne"
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See detailLa fertilité masculine menacée par la présence de perturbateurs endocriniens dans l'environnement ?
Dewalque, Lucas ULg; Charlier, Corinne ULg

in Revue Médicale de Liège (2012), 67(5-6), 243-249

Les perturbateurs endocriniens sont des substances chimiques interférant avec le système hormonal. Ces polluants, présents dans l’environnement, peuvent entraîner l’apparition de maladies chez l’Homme ... [more ▼]

Les perturbateurs endocriniens sont des substances chimiques interférant avec le système hormonal. Ces polluants, présents dans l’environnement, peuvent entraîner l’apparition de maladies chez l’Homme. Dans cet article, nous nous intéressons à ces produits chimiques à effets perturbateurs endocriniens susceptibles d’entraîner une diminution de la qualité du sperme et un syndrome de dysgénésie testiculaire, deux pathologies impliquées dans le déclin de la fertilité masculine. La question de l’influence environnementale sur cette pathologie complexe qu’est l’hypofertilité masculine prend ici tout son sens. [less ▲]

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See detailDifferential Effects of Cocaine on Dopamine Neuron Firing in Awake and Anesthetized Rats
Koulchitsky, Stanislav ULg; DE BACKER, Benjamin ULg; Quertemont, Etienne ULg et al

in Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology (2012), 37

Cocaine (benzoylmethylecgonine), a natural alkaloid, is a powerful psychostimulant and a highly addictive drug. Unfortunately, the relationships between its behavioral and electrophysiological effects are ... [more ▼]

Cocaine (benzoylmethylecgonine), a natural alkaloid, is a powerful psychostimulant and a highly addictive drug. Unfortunately, the relationships between its behavioral and electrophysiological effects are not clear. We investigated the effects of cocaine on the firing of midbrain dopaminergic (DA) neurons, both in anesthetized and awake rats, using pre-implanted multielectrode arrays and a recently developed telemetric recording system. In anesthetized animals, cocaine (10 mg/kg, intraperitoneally) produced a general decrease of the firing rate and bursting of DA neurons, sometimes preceded by a transient increase in both parameters, as previously reported by others. In awake rats, however, injection of cocaine led to a very different pattern of changes in firing. A decrease in firing rate and bursting was observed in only 14% of DA neurons. Most of the other DA neurons underwent increases in firing rate and bursting: these changes were correlated with locomotor activity in 52% of the neurons, but were uncorrelated in 29% of them. Drug concentration measurements indicated that the observed differences between the two conditions did not have a pharmacokinetic origin. Taken together, our results demonstrate that cocaine injection differentially affects the electrical activity of DA neurons in awake and anesthetized states. The observed increases in neuronal activity may in part reflect the cocaine-induced synaptic potentiation found ex vivo in these neurons. Our observations also show that electrophysiological recordings in awake animals can uncover drug effects, which are masked by general anesthesia. [less ▲]

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See detailValidation of the quantitative determination of tetrahydrocannabinol and its two major metabolites in plasma by UHPLC/MS-MS according to the total error approach
DUBOIS, Nathalie ULg; Paccau, A.; DE BACKER, Benjamin ULg et al

in Journal of Analytical Toxicology (2012), 36(1), 25-29

In Belgium, driving under the influence (DUI) of cannabis is prohibited and has severe legal consequences for the driver if the blood plasma concentration of Δ9-tetrahydrocannabinol (THC) exceeds 1 µg/L ... [more ▼]

In Belgium, driving under the influence (DUI) of cannabis is prohibited and has severe legal consequences for the driver if the blood plasma concentration of Δ9-tetrahydrocannabinol (THC) exceeds 1 µg/L. A method to quantify low concentrations of THC and its hydroxylated (THC-OH) and carboxylated (THC-COOH) metabolites in plasma was developed for DUI but also for other applications. Ultra-high-performance liquid chromatography coupled to mass spectrometry seems to be a very convenient method to combine fast chromatographic separation and good sensitivity. The method was validated according to total error approach. Chromatographic separation was achieved in a 3-min total run time. The limits of quantitation were lower or equal to 1 µg/L for all compounds. The linearity of the method was acceptable in the validated range of concentrations (from 0.5 to 50 µg/L for THC, from 0.9 to 50 µg/L for THC-OH and from 1.1 to 100 µg/L for THC-COOH). The biases were lower than 13%, and the relative standard deviations for repeatability and intermediate precision did not exceed 15%. Lower and upper β-expectation tolerance limits did not exceed the acceptance limits of 20% for concentrations higher than 2 µg/L for THC and THC-OH and higher than 4 µg/L for THC-COOH. The acceptance limits were 30% for THC and THC-OH concentrations lower than 2 µg/L and for THC-COOH concentrations lower than 4 µg/L. [less ▲]

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See detailDeterminants of serum concentrations of 1,1 dichloro 2,2 bis (p-chlorophenyl)ethylene and polychlorinated biphenyls among French women in the CECILE study
Bachelet, D.; Truong, T.; Verner, M.-A. et al

in Environmental Research (2011), 111

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See detailLifestyle Behaviours and Plasma Vitamin C and β-Carotene Levels from ELAN Population (Liège, Belgium)
PINCEMAIL, Joël ULg; Vanbelle, Sophie ULg; Degrune, Fabien et al

in Journal of Nutrition and Metabolism (2011), 2011

Several factors, including fruit and vegetables intakes, have been shown to significantly influence the plasma concentrations of the two antioxidants vitamin C and β-carotene. Deficiency levels of 6 mg/L ... [more ▼]

Several factors, including fruit and vegetables intakes, have been shown to significantly influence the plasma concentrations of the two antioxidants vitamin C and β-carotene. Deficiency levels of 6 mg/L (34.2 μM) for vitamin C and of 0.22 mg/L (0.4 μM) for β-carotene have been suggested below which cardiovascular risk might be increased. The present study performed on 897 presumably healthy subjects aged 40–60 years aimed to examine how modifiable lifestyle factors may be related to vitamin C and/or β-carotene deficiency. Gender, smoking, lack of regular physical activity and of daily fruit consumption (≥2/day), and social status (in particular, unemployment) were found to be significant risk factors for vitamin C deficiency. For β-carotene deficiency, the same factors were identified except social status; moreover, overweight and OC use in women were also found to have a deleterious effect. For non exposed subjects, the probability of developing vitamin C deficiency was 4% in men and 2.4% in women. This probability increased to 66.3% for men and to 44.3% for women (and even to 50.4% under OC use), when all risk factors were present. For β-carotene deficiency, the corresponding probabilities were equal to 29.7% in men and 13.7% in women (no risk factor present), and to 86.1% for men and 69.9% (91.6% for OC use) for women (all factors present), respectively [less ▲]

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See detailBlood, urine, and hair kinetic analysis following an acute lead intoxication
Ho, Thi Thanh Giang ULg; KEUTGENS, Aurore ULg; SCHOOFS, Roland et al

in Journal of Analytical Toxicology (2011), 35(1), 60-64

A case of lead exposure resulting from the accidental ingestion of a lead-containing solution is reported. Because of clinical management rapidly performed through chelation therapy by 2,3 ... [more ▼]

A case of lead exposure resulting from the accidental ingestion of a lead-containing solution is reported. Because of clinical management rapidly performed through chelation therapy by 2,3-dimercaptopropane sulfonate sodium and meso-2,3-dimercaptosuccinic acid, blood lead levels of this 51-year-old patient were moderate (412.9 μg/L) and no clinical symptoms were observed. Numerous blood and urine samples were collected for kinetic analysis of lead elimination. However, we report the first case in which hair samples were analyzed to determine the excretion level of lead after acute intoxication. [less ▲]

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See detailToxicologie clinique et médico-légale
Renaux, André; Renaux - Muylkens, Isabelle; Charlier, Corinne ULg et al

in Beauthier, Jean-Pol (Ed.) Traité de Médecine légale (2011)

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See detailQuantification in postmortem blood and identification in urine of tramadol and its two main metabolites in two cases of lethal tramadol intoxication
De Backer, Benjamin ULg; Renardy, Françoise ULg; Denooz, Raphaël ULg et al

in Journal of Analytical Toxicology (2010), 34(9), 599-604

Tramadol has been extensively prescribed for two decades. It is an opioid analgesic considered to induce fewer side effects than other compounds of this class. However, serious complications may occur in ... [more ▼]

Tramadol has been extensively prescribed for two decades. It is an opioid analgesic considered to induce fewer side effects than other compounds of this class. However, serious complications may occur in case of intoxication. We report here two cases of fatal intoxication due to tramadol ingestion. Tramadol, O-desmethyltramadol (ODT) and N-desmethyltramadol (NDT) were quantitatively and qualitatively determined in postmortem blood and urine, respectively. An HPLC method coupled with fluorescence detection was validated using total error approach for the analysis of tramadol, ODT, and NDT in blood. In case1, concentrations of tramadol and its metabolites were 7.7 mg/L (tramadol), 1.33 mg/L (ODT), and 0.6mg/L (NDT). In case 2, concentrations found were 48.34 mg/L (tramadol), 2.43 mg/L (ODT), and 10.09 mg/L (NDT). Opposite ratios of ODT/NDT concentrations observed in different cases were suggested to be useful for the evaluation of the delay between ingestion and death. However, the changes in metabolites levels may also be explained by pharmacokinetic interactions and quantitative differences in the activity of the cytochrome-P450 2D6. Interestingly, norfluoxetine was detected in sub-therapeutic levels in case 2. Most of these aspects in tramadol-related fatalities are reviewed in this paper, and an overview of fatal intoxications due to tramadol is presented. [less ▲]

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See detailInterest of routine dosage of meropenem in difficult to treat infections
Frippiat, Frédéric ULg; Bensahi, Ilham; Denooz, Raphael ULg et al

Poster (2010, October 23)

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See detailTOLERANCE INTERVALS AS CONTROL CHART: COMPARISON TO CLASSIC SHEWHART X̅-R CONTROL CHART
Marini Djang'Eing'A, Roland ULg; Lambert, Véronique; Denooz, Raphael ULg et al

Poster (2010, September)

Detailed reference viewed: 134 (43 ULg)
See detailDétermination de la composition de l'héroïne par UHPLC/MS-MS
DUBOIS, Nathalie ULg; CHARLIER, Corinne ULg

Conference (2010, June)

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See detailUPLC MS/MS method for the determination of bupropion and its main metabolites in human whole blood
Denooz, Raphael ULg; Mercerolle, M.; Lachatre, C. et al

in Journal of Analytical Toxicology (2010), 34(5), 280-286

A selective and sensitive ultra-performance liquid chromatography (UPLC)–electrospray ionization-tandem mass spectrometry (MS) method for simultaneous determination of bupropion and its main metabolites ... [more ▼]

A selective and sensitive ultra-performance liquid chromatography (UPLC)–electrospray ionization-tandem mass spectrometry (MS) method for simultaneous determination of bupropion and its main metabolites, hydroxybupropion, erythrohydrobupropion, and threohydrobupropion, in human whole blood is presented. The sample preparation consists of cleanup protein precipitation with methanol combined with a solid-phase extraction on Oasis HLB cartridges. Analytes were separated on a Waters Acquity UPLC® BEH phenyl column using a binary mobile phase consisting of ammonium formate buffer (2 mM, pH 4) and acetonitrile. Detection was performed on a Waters Acquity UPLC system coupled to a Quattro Premier triple-quadrupole MS in positive ion selected reaction monitoring. Internal standards were bupropion-d9 and hydroxybupropion-d6. Linearity was from 5 to 1000 ng/mL for bupropion and from 10 to 2000 ng/mL for metabolites. Accuracy profiles (80–120%), precision (< 15%), and limits of detection (1 ng/mL for bupropion and 2 ng/mL for metabolites) were also evaluated and responded to all criteria of validation. The aim of this study was to compare this presented method with a previously described method developed on a classic liquid chromatography–tandem MS system. [less ▲]

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See detailQuantitative determination of cannabinoids in serum by UHPLC/MS-MS
Dubois, Nathalie ULg; Paccou, A.; DE BACKER, Benjamin ULg et al

Poster (2010, April)

Detailed reference viewed: 21 (9 ULg)