References of "CAVALIER, Etienne"
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See detailVitamin D: clinical relevance, analytical issues
CAVALIER, Etienne ULg

in Clinical Chemistry & Laboratory Medicine (2011, May), 49(s1), 66

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See detailFatty acids determinations: an cardiovascular risk? Preliminary results
LE GOFF, Caroline ULg; Leroy, Ludovic; Pincemail, Joël ULg et al

in Biomedica - Life Science Summit (2011, April 07)

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See detailFGF23 and Klotho: an emerging role in phosphate metabolism
CAVALIER, Etienne ULg

Conference (2011, April)

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See detailAre the creatinine-based equations accurate to estimate glomerular filtration rate in african american populations ?
DELANAYE, Pierre ULg; MARIAT, Christophe; MAILLARD, Nicolas et al

in Clinical Journal of the American Society of Nephrology (2011), 6

Regarding the high prevalence of African American patients with ESRD, it is important to estimate the prevalence of early stages of chronic kidney disease in this specific population. Because serum ... [more ▼]

Regarding the high prevalence of African American patients with ESRD, it is important to estimate the prevalence of early stages of chronic kidney disease in this specific population. Because serum creatinine concentration is dependent on muscular mass, an ethnic factor has to be applied to creatinine-based equations. Such ethnic factors have been proposed in the Modification of Diet in Renal Disease (MDRD) study equation and in the more recent Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equations. This review analyzes how these correction factors have been developed and how they have, or have not, been validated in external populations. It will be demonstrated that the African American factor in the MDRD study equation is accurate in African American chronic kidney disease (CKD) patients. However, it will be shown that this factor is probably too high for subjects with a GFR of >60 ml/min per 1.73 m2, leading to an underestimation of the prevalence of CKD in the global African American population. It will also be confirmed that this ethnic factor is not accurate in African (non-American) subjects. Lastly, the lack of true external validation of the new CKD-EPI equations will be discussed. Additional trials seem necessary in American African and African populations to better estimate GFR and apprehend the true prevalence of CKD in this population with a high renal risk. [less ▲]

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See detailHuman anti-animal antibodies interference in the Siemens Immulite chemiluminescent insulin immuno-assay: about one case
Cavalier, Etienne ULg; Huberty, Véronique; Carlisi, Ignazia ULg et al

in Clinica Chimica Acta (2011), 412(7-8), 668-669

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See detailParicalcitol for reduction of albuminuria in diabetes
DELANAYE, Pierre ULg; MARIAT, Christophe; KRZESINSKI, Jean-Marie ULg et al

in Journal-Lancet (The) (2011), 377

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See detailFGF-23 et Klotho: une avancée dans le métabolisme du phosphore
CAVALIER, Etienne ULg

Scientific conference (2011, February 10)

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See detailOverestimation of the 25OHD serum concentration with the automated IDS EIA kit.
Cavalier, Etienne ULg; Huberty, V.; Cormier, C. et al

in Journal of Bone and Mineral Research (2011), 26(2), 434-6

We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit ... [more ▼]

We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit adapted on different "open" automated platforms. We performed a comparison between the IDS EIA kit adapted on two different "open"automated platforms and the DiaSorin RIA. We found a systematic bias (higher levels with the IDS EIA kit) for concentrations more than 50-60 ng/mL that was less obvious when the IDS EIA was used in its manual procedure. We thus suggest to use the IDS EIA kit in its manual procedure rather than to adapt it on an automated platform, and to interpret cautiously a 25(OH)D greater than 100 ng/mL with this kit. [less ▲]

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See detailCystatin C is a reliable marker for estimation of glomerular filtration rate in renal transplantation: validation of a new turbidimetric assay using monospecific sheep antibodies
Bargnoux, A. S.; Cavalier, Etienne ULg; Cristol, J. P. et al

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 265-70

Background: The potential use of Cystatin C was recently assessed in kidney transplantation. A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies (Binding Site Human ... [more ▼]

Background: The potential use of Cystatin C was recently assessed in kidney transplantation. A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies (Binding Site Human Cystatin C immunoassay) has been developed. Analytical performance of this new assay was evaluated. Clinical relevance was determined by comparison with a reference method in a cohort of kidney transplant patients. Patients and methods: First, the analytical performance of the Binding Site cystatin C kit was tested on SPAPLUS® and Hitachi® analyzers. Second, a comparison study was performed using SPAPLUS® analyzer against two other cystatin C methods (the Siemens-PENIA method on BNII® and the Dako-PETIA application on Olympus AU640®). Third, the glomerular filtration rate (GFR) was estimated using several predictive cystatin C- and creatinine-based equations and compared to GFR measured by an isotopic method (99mTc-DTPA). These predictive algorithms were analyzed with respect to bias, precision and accuracy. Results: Total intra-assay and inter-assay coefficients of variation were below 5%. Values obtained with the SPAPLUS® correlated with the Siemens-PENIA and the Dako-PETIA methods. The creatinine and cystatin C-based equation allowed reliable assessment of GFR in our population of renal transplantation. Conclusions: The use of algorithms based on cystatin C and creatinine could provide a reliable estimate of GFR in kidney transplantation. [less ▲]

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See detailVitamine D et grossesse
CAVALIER, Etienne ULg

Learning material (2011)

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See detailLe dosage de la vitamine D
CAVALIER, Etienne ULg

Conference (2011, January 28)

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See detailRecommandations pour le choix et l'harmonisation des techniques de dosage de la créatinine
Bargnoux, Anne-Sophie; Boutten, Anne; Cambillau, Michelle et al

in Annales de Biologie Clinique (2011), 69(1), 9-16

En 2010, un groupe de travail mixte constitué de la Société française de biologie clinique (SFBC) et de la Société de nephrologie (SN) a formulé les propositions suivantes afin de réactualiser les ... [more ▼]

En 2010, un groupe de travail mixte constitué de la Société française de biologie clinique (SFBC) et de la Société de nephrologie (SN) a formulé les propositions suivantes afin de réactualiser les recommanadations pour le dosage de la créatinine plasmatique. [less ▲]

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See detailLe dosage de l'inuline: mise au point
DELANAYE, Pierre ULg; Souvignet, Marie; Dubourg, Laurence et al

in Annales de Biologie Clinique (2011), 69(3), 273-84

L’inuline, polymère de fructose, reste le marqueur de référence du débit de filtration glomérulaire (DFG). Popularisé par les études de Smith et Shannon, son dosage n’en demeure pas moins complexe et ... [more ▼]

L’inuline, polymère de fructose, reste le marqueur de référence du débit de filtration glomérulaire (DFG). Popularisé par les études de Smith et Shannon, son dosage n’en demeure pas moins complexe et sujet à des interférences dont celle du glucose est la plus importante. Il existe deux grands types de dosages de l’inuline : les méthodes de dosage « acide » et enzymatiques. Le dosage « acide » consiste en un dosage colorimétrique du fructose obtenu après hydrolyse de l’inuline en milieu très acide. Le dosage du fructose englobe différentes méthodes de dosage dont la plus utilisée est la réaction à l’anthrone. Toutes ces méthodes présentent des interférences au glucose. Différentes méthodes « enzymatiques » ont été décrites au cours du temps. Celles-ci apparaissent plus précises et sans doute moins sujettes aux interférences même si peu d’études comparatives sont disponibles. Plusieurs auteurs ont également développé des dosages de type CLHP. Cette méthode spécifique et précise demeure cependant moins adaptée à la routine. Ainsi, si l’utilisation de l’inuline comme marqueur de référence n’est pas remise en cause, son dosage reste délicat, sujet aux interférences et à une certaine interprétation. Des études supplémentaires restent nécessaires pour valider analytiquement et comparativement les techniques de dosage de l’inuline. [less ▲]

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See detailDrospirenone and interference with renin-antiotensin system
VROONEN, Laurent ULg; Cavalier, Etienne ULg; Vranken, L. et al

in Annales d'Endocrinologie - 28ème congrès de la société Française d'Endocrinologie (2011)

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See detailSeasonal variations in vitamin D levels in melanoma patients : a single-centre prospective pilot comparative study
FAILLA, Valérie ULg; CAVALIER, Etienne ULg; EL HAYDERI, Lara ULg et al

in Journal of the European Academy of Dermatology & Venereology (2011)

Background: More than 90% of vitamin D synthesis is dependent on UV exposure. Photosensitive disorders such as lupus erythematosus, protoporphyria and xeroderma require strict sun avoidance, and vitamin D ... [more ▼]

Background: More than 90% of vitamin D synthesis is dependent on UV exposure. Photosensitive disorders such as lupus erythematosus, protoporphyria and xeroderma require strict sun avoidance, and vitamin D deficiency has been demonstrated in these patients. Melanoma patients are also instructed to avoid sun exposure and may hence be expected to be vitamin D deficient. Materials and method : Winter and summer vitamin D levels were compared in a group of melanoma patients (n=61) and age- and phototype-matched controls (n=53) without photosensitive disorders. Results : Oral supplementary vitamin D intake was reported in 32.7% of the melanoma patients and in 15.1 % in the control group. Despite oral supplementation, only 25% of the melanoma patients and the controls presented with vitamin D levels of 30 ng/mL or higher. In non-supplemented subjects in the melanoma and control groups, respectively, mean winter vitamin D levels were below the recommended threshold at 12,6 ng/mL vs 13.2 ng/mL, respectively, but not statistically different. These values increased significantly in both groups during the summer to 24.6 and 23.8 ng/mL respectively. Conclusion: Unexpected, significant increases in vitamin D levels were seen in melanoma patients during summer, suggesting non-adherence with photoprotective measures and reflecting a heliophilic behaviour. Vitamin D supplementation is recommended in melanoma patients during both winter and summer. [less ▲]

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See detailA multicentric evaluation of IDMS-traceable creatinine enzymatic assays
Pieroni, Laurence; DELANAYE, Pierre ULg; Boutten, Anne et al

in Clinica Chimica Acta (2011), 412

Chronic kidney disease definition is based on glomerular filtration rate (GFR) estimations which are derived from creatinine-based equations. The accuracy of GFR estimation is thus largely dependent of ... [more ▼]

Chronic kidney disease definition is based on glomerular filtration rate (GFR) estimations which are derived from creatinine-based equations. The accuracy of GFR estimation is thus largely dependent of those of serum creatinine assays. International recommendations highlight the need for traceable creatinine assays. The French Society of Clinical Biochemistry conducted a study for measuring accuracy of creatinine enzymatic methods. This evaluation involved 25 clinical laboratories. Creatinine was measured in serum pools ranging from 35.9±0.9 μmol/L to 174.5±3.1 μmol/L (IDMS determination) using 12 creatinine enzymatic methods. For all creatinine values greater than 74.4±1.4 μmol/L, the bias and imprecision did not exceed 5% and 5.9%, respectively. For the lowest value (35.9±0.9 μmol/L), the bias ranged from −1.8 to 9.9% (with one exception). At this level, the imprecision ranged from 1.9 to 7.8%. The true performances of the assays (couples of bias and relative standard deviation), were evaluated using Monte-Carlo simulations. Most of the assays fall within the maximum Total Error of 12% at all concentrations. This study demonstrates substantial improvements in the calibration, traceability and precision of the enzymatic methods, reaching the NKDEP recommendations. Moreover, most of these assays allowed accurate creatinine measurements for creatinine levels lower than 40 μmol/L. [less ▲]

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See detailValidation of the Abbott Architect 25(OH)-vitamin D assay
CAVALIER, Etienne ULg; CARLISI, Ignazia ULg; BEKAERT, Anne-Catherine ULg et al

in Clinical Chemistry & Laboratory Medicine (2011), 49(s1), 418

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