References of "Busoni, Valeria"
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See detailULTRASONOGRAPHIC FINDINGS IN 13 HORSES WITH LYMPHOMA
Janvier, Valentin; Evrard, Laurence ULg; Cerri, Simona ULg et al

in Veterinary Radiology & Ultrasound (in press)

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See detailCOMPARISON OF ULTRASOUND-GUIDED VS. STANDARD LANDMARK TECHNIQUES FOR TRAINING NOVICE OPERATORS IN PLACING NEEDLES INTO THE LUMBAR SUBARACHNOID SPACE OF CANINE CADAVERS
Etienne, Anne-Laure ULg; Delguste, Catherine ULg; Busoni, Valeria ULg

in Veterinary Radiology & Ultrasound (2016)

The standard technique for placing a needle into the canine lumbar subarachnoid space is primarily based on palpation of anatomic landmarks and use of probing movements of the needle, however, this ... [more ▼]

The standard technique for placing a needle into the canine lumbar subarachnoid space is primarily based on palpation of anatomic landmarks and use of probing movements of the needle, however, this technique can be challenging for novice operators. The aim of the current observational, prospective, ex vivo, feasibility study was to compare ultrasound-guided vs. standard anatomic landmark approaches for novices performing needle placement into the lumbar subarachnoid space using dog cadavers. Eight experienced operators validated the canine cadaver model as usable for training landmark and ultrasound-guided needle placement into the lumbar subarachnoid space based on realistic anatomy and tissue consistency. With informed consent, 67 final year veterinary students were prospectively enrolled in the study. Students had no prior experience in needle placement into the lumbar subarachnoid space or use of ultrasound. Each student received a short theoretical training about each technique before the trial and then attempted blind landmark-guided and ultrasound-guided techniques on randomized canine cadavers. After having performed both procedures, the operators completed a self-evaluation questionnaire about their performance and self-confidence. Total success rates for students were 48% and 77% for the landmark- and ultrasound-guided techniques, respectively. Ultrasound guidance significantly increased total success rate when compared to the landmark-guided technique and significantly reduced the number of attempts. With ultrasound guidance self-confidence was improved, without bringing any significant change in duration of the needle placement procedure. Findings indicated that use of ultrasound guidance and cadavers are feasible methods for training novice operators in needle placement into the canine lumbar subarachnoid space. [less ▲]

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See detailLearning to look - Purpose and design of an awareness-raising online course in veterinary sciences
Tasnier, Sophie ULg; Busoni, Valeria ULg; Hanzen, Christian ULg et al

in Prilla, M; Ullmann, T; Kravcik, M (Eds.) et al Proceedings of the 5th Workshop on Awareness and Reflection in Technology-Enhanced Learning (2015, September 15)

This paper reports on a work in progress: an online self-instruction course created to stimulate students’ awareness processes when dealing with pictures. Using non-clinical material, the “Learning to ... [more ▼]

This paper reports on a work in progress: an online self-instruction course created to stimulate students’ awareness processes when dealing with pictures. Using non-clinical material, the “Learning to Look” course was de-signed as a preliminary training to the observation of histological sections, radi-ological graphs, and other specialized visual material. Following a presentation of the project, salient results of a feedback questionnaire completed by 382 stu-dents about their experience of the course are provided. [less ▲]

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See detailComparaison de l’effet du guidage échographique sur les ponctions de liquide céphalo-rachidien entre les localisations cisternales et lombaires chez le chien : une étude ex vivo
Etienne, Anne-Laure ULg; Peeters, Dominique ULg; Busoni, Valeria ULg

in Annales de Médecine Vétérinaire (2015), 158

RÉSUMÉ : Le placement échoguidé de l’aiguille spinale pour les ponctions cisternale et lombaire chez le chien a été décrit. L’objectif de ce travail était de comparer l’effet du guidage échographique pour ... [more ▼]

RÉSUMÉ : Le placement échoguidé de l’aiguille spinale pour les ponctions cisternale et lombaire chez le chien a été décrit. L’objectif de ce travail était de comparer l’effet du guidage échographique pour la ponction de liquide céphalo-rachidien entre les ponctions cisternale et lombaire. Quatre-vingt-trois opérateurs ont participé à l’étude. Ils étaient divisés en quatre groupes selon leur expérience. Après une formation théorique courte, chaque opérateur a réalisé une ponction cisternale et lombaire en aveugle et sous contrôle échographique sur des cadavres tirés au sort. L’échoguidage lombaire était direct alors que celui cisternal était indirect. Après chaque procédure, les opérateurs ont rempli un questionnaire sur leur expérience, leur performance et leur confiance en soi. L’échoguidage augmentait significativement le taux de réussite global de la ponction (comparée à la méthode en aveugle) mais plus clairement avec la localisation lombaire. Le guidage échographique cisternal améliorait significativement le nombre de tentatives, le temps et la confiance en soi chez les opérateurs inexpérimentés et expérimentés. La première cause d’échec citée était différente selon la localisation de la ponction et était liée à la nature de l’échoguidage. Cette étude démontre que les opérateurs inexpérimentés sont capables de réaliser des ponctions échoguidées après une formation théorique courte, justifiant l’utilité du guidage échographique à des fins pédagogiques. [less ▲]

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See detailFeasibility of ultrasound-guided epidural access at the lumbo-sacral space in dogs
Liotta, Annalisa Pia ULg; Busoni, Valeria ULg; Carrozzo et al

in Veterinary Radiology & Ultrasound (2015), 56(2), 220-228

Epidural injections are commonly performed blindly in veterinary medicine. The aims of this study were to describe the lumbosacral ultrasonographic anatomy and to assess the feasibility of an ultrasound ... [more ▼]

Epidural injections are commonly performed blindly in veterinary medicine. The aims of this study were to describe the lumbosacral ultrasonographic anatomy and to assess the feasibility of an ultrasound-guided epidural injection technique in dogs. A cross sectional anatomic atlas of the lumbosacral region and ex vivo ultrasound images were obtained in two cadavers to describe the ultrasound anatomy and to identify the landmarks. Sixteen normal weight canine cadavers were used to establish two variations of the technique for direct ultrasound-guided injection, using spinal needles or epidural catheters. The technique was finally performed in two normal weight cadavers, in two overweight cadavers and in five live dogs with radiographic abnormalities resulting of the lumbosacral spine. Contrast medium was injected and CT was used to assess the success of the injection. The anatomic landmarks to carry out the procedure were the seventh lumbar vertebra, the iliac wings, and the first sacral vertebra. The target for directing the needle was the trapezoid-shaped echogenic zone between the contiguous articular facets of the lumbosacral vertebral canal visualized in a parasagittal plane. The spinal needle or epidural catheter was inserted in a 45° craniodorsal-caudoventral direction through the subcutaneous tissue and the interarcuate ligament until reaching the epidural space. CT examination confirmed the presence of contrast medium in the epidural space in 25/25 dogs, although a variable contamination of the subarachnoid space was also noted. Findings indicated that this ultrasound-guided epidural injection technique is feasible for normal weight and overweight dogs, with and without radiographic abnormalities of the spine. [less ▲]

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See detailLordose et/ou xyphose chez le porc : mise à l’épreuve de l’hypothèse héréditaire
Laitat, Martine ULg; Veillat, Emilie; Van Cauwenberge, Henry et al

Poster (2015, February)

Lordosis and/or kyphosis, also called ”dipped shoulder” or ”humpy‐back” is sporadically observed in growing pigs. This condition is characterized by a thoracic and/or lumbar spinal deformity ... [more ▼]

Lordosis and/or kyphosis, also called ”dipped shoulder” or ”humpy‐back” is sporadically observed in growing pigs. This condition is characterized by a thoracic and/or lumbar spinal deformity. Pathomorphologically, it may be comparable with Scheuermann’s kyphosis in man and so constitutes a spontaneous model for this humane kyphosis of the thoracic or thoracolumbar spine. In pigs, this condition may decrease the value of carcasses, making deboning efforts challenging. Three major and non‐exclusive hypotheses formulated to explain these back deformations are nutrition, intrauterine viral infection and inherited predisposition. The objective of the present study was to test the latter and, if possible, to identify a locus (some loci) associated with the affection. Forty‐eight pigs were included in this case‐control study. Based on a clinical examination and/or on a measure of the degree of spinal deformity, 25 pigs classified as affected were compared to 23 pigs considered as normal. A whole genome Single Nucleotide Polymorphism (SNP) analysis was performed using a 50,000 SNP array. DNA from forty‐seven samples (tail tissue or blood) was extracted while one sample was eliminated because of its poor quality. After applying quality controls, 40 pigs and 57,838 SNPs (on a total of 62,163) remained for further analysis. One SNP (ASGA0090747) located on Sus scrofa chromosome SSC8 crossed the genome‐wide significant threshold and is thus suspected of being associated with the lordosis and/or kyphosis phenotype. These results seem to confirm the hereditary hypothesis. Further investigations are however needed to confirm the suspected association. [less ▲]

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See detailL’imagerie du thorax et de l’abdomen.
Busoni, Valeria ULg

in Maladies des chevaux (2015)

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See detailDEVELOPING A TECHNIQUE FOR ULTRASOUND-GUIDED INJECTION OF THE ADULT CANINE HIP.
Bergamino, Chiara; Etienne, Anne-Laure ULg; Busoni, Valeria ULg

in Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association (2015)

An accurate method for guiding injections into the canine hip would facilitate diagnostic localization of lameness and targeted treatments. Ultrasound-guided hip injections are commonly used in humans and ... [more ▼]

An accurate method for guiding injections into the canine hip would facilitate diagnostic localization of lameness and targeted treatments. Ultrasound-guided hip injections are commonly used in humans and large animals. Aims of this prospective study were to describe ultrasound (US) anatomy of the adult canine hip and determine the feasibility and accuracy of intra-articular placement of injectate using US-guidance. Seven adult dogs were used to describe US anatomy, five dog cadavers were used to assess the feasibility of the injection technique and 11 dog cadavers were used to assess accuracy of injections. For the accuracy test, 22 joints were injected with iodinated contrast medium by three operators with different experience. With dogs in lateral recumbency, the hyperechoic femoral head surface was identified by following the femoral neck from the greater trochanter or the acetabular rim was localized by following caudally the ilium from the iliac wing. An anechoic gap between the femoral head and acetabular surface represented the joint. The capsule was visible as a triangular echoic structure and the femoral head articular cartilage appeared as an anechoic band. The needle was inserted axial to the greater trochanter and directed in a dorsolateral-ventromedial direction toward the joint space and then pushed through the capsule. Based on postinjection radiography, accuracy was 81.8% at first attempt and 100% at second attempt. This study indicated that US-guided injection is a feasible and accurate technique for injecting the adult canine hip. Future studies in live dogs are needed to assess safety and efficacy. [less ▲]

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See detailLordosis in pigs: testing the hereditary hypothesis.
Laitat, Martine ULg; Van Cauwenberge, H; Harmegnies, Natacha ULg et al

Poster (2014)

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See detailSur la mise en place d’un module d’initiation à la Pleine Conscience destiné aux étudiants en Médecine Vétérinaire
Busoni, Valeria ULg

Master's dissertation (2014)

Ce mémoire de Certificat Universitaire (formation continue) reprend les données relatives à la mise en place d'un module d'initiation à la pleine conscience à destination des étudiants en médecine ... [more ▼]

Ce mémoire de Certificat Universitaire (formation continue) reprend les données relatives à la mise en place d'un module d'initiation à la pleine conscience à destination des étudiants en médecine vétérinaire. [less ▲]

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See detailRadiographic evaluations of distal navicular border fragments and forefeet conformation in the horse
Careddu, Gian Mario; Cubeddu, Francesca; Dore, S et al

Conference (2014)

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See detailStéatite généralisée chez 3 chevaux adultes référés à l’université de Liège
Leroux, Aurélia ULg; Garcia da Fonseca, Rita; Bayrou, Calixte ULg et al

in Pratique Vétérinaire Equine (2014), 184

In horses, two forms of generalised steatitis have been described: one subcutaneous nodular form and one diffuse systemic form. Three cases of the second form are described in this paper. The clinical ... [more ▼]

In horses, two forms of generalised steatitis have been described: one subcutaneous nodular form and one diffuse systemic form. Three cases of the second form are described in this paper. The clinical signs included depression, anorexia, diarrhoea, abdominal pain, fever, subcutaneous oedema, tachycardia and tachypnea. Blood analyses showed anemia, neutrophilia, hypoalbuminemia, increased muscular enzymes activities, and low level of vitamine E concentration. Abdominal ultrasound demonstrated an irregular hyperechoic band of peritoneal fat tissue. Ante mortem diagnosis was achieved in 2 horses, one on the basis of rectal biopsies and one on the basis of biopsies of the nuchal ligament. Despite a corticoids therapy and vitamin E supplementation, the 3 horses died or were euthanized. Those cases confirm that, in horses, diagnosis of systemic diffuse generalised steatitis is difficult and that the prognosis is poor. [less ▲]

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See detailUltrasound-guided epidural access in dog
Liotta, Annalisa Pia ULg; Sandersen, Charlotte ULg; Busoni, Valeria ULg et al

in Veterinary Radiology and ultrasound (2013, August 29)

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See detailULTRASOUND ANATOMY AND US-GUIDED INTRA-ARTICULAR INJECTION TECHNIQUE OF THE CANINE ADULT HIP
Bergamino, Chiara; Etienne, Anne-Laure ULg; Busoni, Valeria ULg

Poster (2013, August)

Introduction Ultrasound (US)-guided techniques are routinely used in human1,2 and veterinary3 medicine to increase safety and accuracy1. Due to its deep location blind needle insertion in the coxo-femoral ... [more ▼]

Introduction Ultrasound (US)-guided techniques are routinely used in human1,2 and veterinary3 medicine to increase safety and accuracy1. Due to its deep location blind needle insertion in the coxo-femoral joint may be difficult. The aim of this study was to determine the US anatomy of the adult canine hip in order to establish the approach for US-guided intra-articular (IA) injections. Materials and Methods A 3-18 MHz linear and a 5-10 curvilinear transducers were used. Seven adult live dogs were used to  establish the US anatomy. Ten dog cadavers were used to obtain frozen sections to compare to US images and to establish the injection technique. Dogs were clipped and examined in lateral recumbency using a dorsal approach. To assess accuracy of injections performed on cadavers, joints were injected with a iodinated contrast medium and controlled radiographically. Injections were realized by 3 operators with different US experience using a 22G needle. To localize the joint, two techniques were established: one using the greater trocanther as landmark, one using the ilium wing. Results The coxo-femoral joint was easily visualized in all live dogs and cadavers. When the great trochanter was used as anatomical landmark, the femoral neck hyperechoic surface was identified in continuity with the greater trocanther hyperechoic surface in a plane perpendicular to the vertebral column and then followed axially till the anechoic gap representing the articular space. At the joint space the hyperechoic acetabulum was seen partially covering the femoral head and the joint capsule was visible as a triangular echoic structure (Figs. 1 & 2). Using the hyperechoic surface of the ilium wing as a starting point, the coxofemoral joint was localized following the wing and the hyperechoic ilium body caudally in a transverse plane. The inexperienced operator found easier to localize the joint using the ilium as landmark. For injection the needle was inserted axially to the great trochanter and directed in a dorsolateral-ventromedial direction toward the joint space and then pushed through the capsule (Figs. 3 & 4). Twenty hips were injected (Fig. 5). In all cases operators had the feeling to pass the joint capsule with the needle. All injections were successful and control radiographs showed intra-articular contrast (Fig. 6). Discussion/Conclusion The dorsal aspect of the canine hip can be easily localized at US and US-guidance can be efficiently used for IA injection [less ▲]

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See detailMorphometric analyses of the normal suspensory ligament in Standardbreds
Shikh Al Sook, Mohamad Khir ULg; Antoine, Nadine ULg; Piret, Joëlle ULg et al

Poster (2013, July)

The suspensory ligament (SL) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the SL in ... [more ▼]

The suspensory ligament (SL) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the SL in sound horses. Right limbs from 11 horses were collected. Samples from 6 levels of the SL were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. Using the cryosection, some artefacts appeared. Cryoprotection was carried out, which produced the best results. Hematoxylin–phloxine–saffron and Hematoxylin–eosin gave a good contrast of colours between the tissues allowing the use of an image analysis programme. The percentage of MT and AT decreased significantly (P < 0.0001), whereas the percentage of CT increased significantly (P < 0.0001) with age and when descending from the proximal to the distal level of the SL. The percentage of MT was significantly higher (P < 0.0001) in females than males, while the percentage of CT was significantly higher (P < 0.0001) in males than females. The percentage of AT was significantly higher (P = 0.0278) in pelvic limbs than in thoracic limbs. These results confirm the variation in tissue composition within the SL of sound horses. [less ▲]

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See detailUltrasonographic characteristics of the cisterna chyli in eight dogs and four cats
Etienne, Anne-Laure ULg; Cavrenne, Romain ULg; Gommeren, Kris ULg et al

in Veterinary Radiology & Ultrasound (2013), 54(4), 398-402

Ultrasonography of the cisterna chyli has been used in humans to diagnose increased lymphatic flow or lymph flow obstruction and to guide percutaneous embolization of the thoracic duct via the cisterna ... [more ▼]

Ultrasonography of the cisterna chyli has been used in humans to diagnose increased lymphatic flow or lymph flow obstruction and to guide percutaneous embolization of the thoracic duct via the cisterna chyli. The aim of this study was to describe the ultrasonographic characteristics of the dorsal portion of cisterna chyli in dogs and cats with chylous ascites or chylothorax and in a group of healthy dogs and cats. The aorta and the cranial mesenteric artery were used as anatomic landmarks. Ultrasonography was performed before and 2 h after a fatty meal in healthy dogs and cats. The visualized structure was confirmed to be a dilated cisterna chyli at necropsy in a dog with chylous ascites. The confirmed or presumed cisterna chyli was consistently detected using ultrasonography in nonfasted healthy animals and clinically affected animals and appeared as an anechoic tubular structure, without detectable flow, at the right dorsolateral aspect of the aorta. It had a similar ultrasonographic appearance in patients with chyloabdomen and in nonfasted healthy dogs and cats. There was considerable overlap in diameters of the cisterna chyli for affected and healthy animals. The shape and size of the cisterna chyli in an individual animal were variable during the same ultrasound examination and between different examinations. This study demonstrated the appearance of the presumed dorsal portion of the cisterna chyli by ultrasonography and might provide useful preliminary data for further studies into the feasibility of ultrasound-guided injections or aspirations of the cisterna chyli in dogs and cats. [less ▲]

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