References of "Bureau, Fabrice"
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See detailInterleukin-6/STAT3 signaling regulates the ability of naive T cells to acquire B-cell help capacities
Eddahri, Fouad; Denanglaire, Sebastien; Bureau, Fabrice ULg et al

in Blood (2009), 113(11), 2426-2433

The conditions leading to the activation/differentiation of T-helper (Th) cells dedicated for B-cell antibody production are still poorly characterized. We now demonstrate that interleukin-6 (IL-6 ... [more ▼]

The conditions leading to the activation/differentiation of T-helper (Th) cells dedicated for B-cell antibody production are still poorly characterized. We now demonstrate that interleukin-6 (IL-6) promotes the differentiation of naive T lymphocytes into helper cells able to promote B-cell activation and antibody secretion. IL-6-driven acquisition of B-cell help capacity requires expression of the signal transducer and activator of transcription 3 (STAT3), but not STAT4 or STAT6 transcription factors, suggesting that the ability to provide help to B cells is not restricted to a well-defined Th1 or Th2 effector population. T cell-specific STAT3-deficient mice displayed reduced humoral responses in vivo that could not be related to an altered expansion of CXCR5-expressing helper T cells. IL-6 was shown to promote IL-21 secretion, a cytokine that was similarly found to promote the differentiation of naive T cells into potent B-cell helper cells. Collectively, these data indicate that the ability to provide B-cell help is regulated by IL-6/IL-21 through STAT3 activation, independently of Th1, Th2, Th17, or follicular helper T cell (T(FH)) differentiation [less ▲]

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See detailPTX3: a new marker for local inflammation ?
Ramery, Eve ULg; Bureau, Fabrice ULg; Art, Tatiana ULg et al

in Proceedings of the 11th ECVCP congress, Thessaloniki, Grece (2009)

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See detailDust exposure exacerbates the expression of ptx3, a recently discovered acute phase protein, in horses’ airways
Ramery, Eve ULg; Bureau, Fabrice ULg; Lekeux, Pierre ULg

Conference (2009)

Heaves is a common cause of recurrent airway obstruction (R.A.O) that occurs in mature horses stabled in a dusty environment. The long pentraxin PTX3 play an important role in the host defence and over ... [more ▼]

Heaves is a common cause of recurrent airway obstruction (R.A.O) that occurs in mature horses stabled in a dusty environment. The long pentraxin PTX3 play an important role in the host defence and over-expression of PTX3 may contribute to airways injury. Therefore, we hypothesised that PTX3 may have relevance in the understanding of the pathogenesis of recurrent airway obstruction (R.A.O). In the present study, we first investigated the equine PTX3 (ePTX3) structure by cloning, sequencing and western-blotting. A 120pb deletion was found in the second exon of ePTX3 in comparison to human PTX3. Moreover, an alternative splicing occurs with complete deletion of the second exon, resulting in two forms of the protein, “spliced” (32 kD) and “full length” (42 kD). The pentraxin domain is very well conserved in the two forms with 94% of amino-acids conserved between equines and humans. These findings could have implications for the comprehension of the function of each domain of the protein. Then, we studied the effects of dust exposure and subsequent R.A.O crisis on ePTX3 expression in the broncho-alveolar lavage fluid (BALF) and the bronchial epithelial cells. PTX3 could be detected in BALF macrophages, neutrophils, eosinophils and bronchial epithelial cells. It was over-expressed in the BALF supernatant from RAO-affected horses in crisis. Dust exposure induced PTX3 in BALF macrophages either in healthy horses or in R.A.O-affected horses. Conversely, PTX3 was strongly over-expressed in the bronchial epithelial cells from R.A.O-affected horses in crisis. Considering epithelial cells as one of the major cell types in the airways, PTX3 produced at this level may play an important role in inflammatory process. Moreover, it suggests that dust-induced expression of PTX3 is differentially regulated in macrophages and bronchial-epithelial cells. A JNK-dependant, NFκB-independent regulatory pathway has been described in lung epithelial cells. A comparable mechanism could be involved in bronchial epithelial cells. If this is confirmed, PTX3 expression in respiratory epithelial cells could be therapeutically targeted. [less ▲]

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See detailHow transcriptomic studies may help to improve the control of bovine diseases : an example with calf pneumonia and endotoxemia
Wallemacq, Hugues ULg; Ramery, Eve ULg; Bureau, Fabrice ULg et al

in Proceedings: XXVth Jubilee World Buiatrics Congress (2008)

Global change in genes expression induced by pathological processes can now be analysed in cattle by new tools called microarrays. These transcriptomic studies may help to better understand the ... [more ▼]

Global change in genes expression induced by pathological processes can now be analysed in cattle by new tools called microarrays. These transcriptomic studies may help to better understand the pathophysiological mechanisms responsible for the disease and may therefore contribute to develop more efficient preventive and curative strategies. An example is given with a model of calf pneumonia and endotoxemia [less ▲]

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See detailLung interstitial macrophages prevent lipopolysaccharide-triggered T helper type 2 responses to harmless inhaled antigens
Bedoret, D.; Wallemacq, Hugues ULg; Marichal, Thomas ULg et al

in Proceedings of the Annual BIS-meeting (2008)

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See detailIr-LBP, an ixodes ricinus tick salivary LTB4-binding lipocalin, interferes with host neutrophil function.
Beaufays, Jérôme ULg; Adam, Benoit; Menten-Dedoyart, Catherine ULg et al

in PLoS ONE (2008), 3(12), 3987

BACKGROUND: During their blood meal, ticks secrete a wide variety of proteins that can interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation ... [more ▼]

BACKGROUND: During their blood meal, ticks secrete a wide variety of proteins that can interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation of the inflammatory response. METHODOLOGY/PRINCIPAL FINDINGS: We previously identified 14 new lipocalin genes in the tick Ixodes ricinus. One of them codes for a protein that specifically binds leukotriene B4 with a very high affinity (Kd: +/-1 nM), similar to that of the neutrophil transmembrane receptor BLT1. By in silico approaches, we modeled the 3D structure of the protein and the binding of LTB4 into the ligand pocket. This protein, called Ir-LBP, inhibits neutrophil chemotaxis in vitro and delays LTB4-induced apoptosis. Ir-LBP also inhibits the host inflammatory response in vivo by decreasing the number and activation of neutrophils located at the tick bite site. Thus, Ir-LBP participates in the tick's ability to interfere with proper neutrophil function in inflammation. CONCLUSIONS/SIGNIFICANCE: These elements suggest that Ir-LBP is a "scavenger" of LTB4, which, in combination with other factors, such as histamine-binding proteins or proteins inhibiting the classical or alternative complement pathways, permits the tick to properly manage its blood meal. Moreover, with regard to its properties, Ir-LBP could possibly be used as a therapeutic tool for illnesses associated with an increased LTB4 production. [less ▲]

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See detailUsing a human microarray to highlight new genes of interest for a better understanding of molecular mechanisms that underpin the physiopathology of heaves
Ramery, Eve ULg; Closset, Rodrigue; Bureau, Fabrice ULg et al

in Proceedings: Plant and Animal Genome Conference, Equine Workshop, San Diego (2008)

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Using reverse transcription polymerase chain reaction (RT-PCR), disparate results have been ... [more ▼]

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Using reverse transcription polymerase chain reaction (RT-PCR), disparate results have been obtained concerning cytokines expression profile. cDNA microarray appears to be so far the platform of choice for massively parallel gene expression profiling and provides a good tool for exploratory research. However, equine-specific microarrays are not yet available on the market. Because they are commercially available, highly specific and well annotated, human and mouse large-scale microarrays are an exploratory alternative to equine-specific microarrays. In the present study, the purpose was to highlight new targets not previously related to the disease and able to improve our understanding of the molecular mechanisms of the disease. A human microarray was used to study gene expression in nucleated cells originating from peripheral blood and bronchoalveolar lavage fluid in heaves-affected horses. With a four-fold cut-off, a total of 46 candidates were identified with differentially regulated genes between heaves-affected horses and controls. Based on their documented function, five of these genes were selected for the real-time quantitative RT-PCR (RT-qPCR) validation procedure: CYBB, BTG1, MARCKS, PTX3 and PTPRC. The RT-qPCR results confirmed those obtained with the microarray, pointing out these genes as new directions for future experiments. However, the human microarray failed to detect the presence of IL-1beta and Il-8, otherwise confirmed by RT-qPCR, and the expression profile of the disease could not be obtained [less ▲]

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See detailAnti-inflammatory effects of ipratropium bromide in rats with cadmium-induced acute pulmonary inflammation
Zhang, W.; Fievez, Laurence ULg; Cheu, Esteban ULg et al

in Proceedings of the 12th Annual Meeting of the French Society of Pharmacology and Therapeutics (2008)

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See detailRole of beta 2-receptors in the anti-inflammatory effects of formoterol in rats with cadmium-induced acute pulmonary inflammation
Zhang, W.; Fievez, Laurence ULg; Cheu, Esteban ULg et al

in Fundamental & Clinical Pharmacology (2008), 227

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See detailExpression génique et médecine vétérinaire
Ramery, Eve ULg; Van Erck, E.; Bureau, Fabrice ULg et al

in 36èmes Journées AVEF (2008)

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See detailHunting for a key to the enigma of heaves in the black box of the white cells
Art, Tatiana ULg; Bureau, Fabrice ULg; Robinson, N Edward

in Veterinary Journal (2008), 177(3), 307-308

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See detailDendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma.
Henry, E.; Desmet, Christophe ULg; Garze, V. et al

in Journal of Immunology (2008), 181(10), 7230-7242

Dendritic cells (DCs) are professional APCs that have a unique capacity to initiate primary immune responses, including tolerogenic responses. We have genetically engineered bone marrow-derived DCs to ... [more ▼]

Dendritic cells (DCs) are professional APCs that have a unique capacity to initiate primary immune responses, including tolerogenic responses. We have genetically engineered bone marrow-derived DCs to express the immunosuppressive cytokine IL-10 and tested the ability of these cells to control experimental asthma. A single intratracheal injection of OVA-pulsed IL-10-transduced DCs (OVA-IL-10-DCs) to naive mice before OVA sensitization and challenge prevented all of the cardinal features of airway allergy, namely, eosinophilic airway inflammation, airway hyperreactivity, and production of mucus, Ag-specific Igs, and IL-4. OVA-IL-10-DCs also reversed established experimental asthma and had long-lasting and Ag-specific effects. We furthermore showed, by using IL-10-deficient mice, that host IL-10 is required for mediating the immunomodulatory effects of OVA-IL-10-DCs and demonstrated a significant increase in the percentage of OVA-specific CD4(+)CD25(+)Foxp3(+)IL-10(+) regulatory T cells in the mediastinal lymph nodes of OVA-IL-10-DC-injected mice. Finally, adoptive transfer of CD4(+) mediastinal lymph node T cells from mice injected with OVA-IL-10-DCs protected OVA-sensitized recipients from airway eosinophilia upon OVA provocation. Our study describes a promising strategy to induce long-lasting Ag-specific tolerance in airway allergy. [less ▲]

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See detailRelevance of using a human microarray to study gene expression in heaves-affected horses.
Ramery, Eve ULg; Closset, Rodrigue; Bureau, Fabrice ULg et al

in Veterinary Journal (2008), 177(2), 216-221

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Previous studies have highlighted the implications of variations in gene expression, most ... [more ▼]

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Previous studies have highlighted the implications of variations in gene expression, most using reverse transcription polymerase chain reaction (RT-PCR). This well-known technique limits the number of genes that can be studied in a single assay. Microarray appears to be a valuable tool to by-pass this limitation, but so far there has been no equine-specific microarray available on the market. The present study was performed to determine whether a human microarray could be used to study gene expression in nucleated cells originating from peripheral blood and bronchoalveolar lavage fluid (BALF) in heaves-affected horses. With a four-fold cut-off, a total of 46 candidates were identified with differentially regulated genes between heaves-affected horses and controls. A real-time quantitative RT-PCR (RT-QPCR) conducted on a selection of genes, determined on the basis of previous publications, was used to validate the microarray results. The microarray failed to detect the presence of interleukin (IL)-1beta and IL-8 mRNA in the nucleated cells from BALF otherwise confirmed by real-time RT-QPCR. Although some candidate genes have been identified using this method, a complete expression profile of genes related to heaves could not be obtained with the use of the human microarray. [less ▲]

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See detailNicotinamide phosphoribosyl transferase/pre-B cell colony-enhancing factor/visfatin is required for lymphocyte development and cellular resistance to genotoxic stress.
Rongvaux, Anthony; Galli, Mara; Denanglaire, Sebastien et al

in Journal of Immunology (2008), 181(7), 4685-4695

Nicotinamide phosphoribosyl transferase (Nampt)/pre-B cell colony-enhancing factor (PBEF)/visfatin is a protein displaying multiple functional properties. Originally described as a cytokine-like protein ... [more ▼]

Nicotinamide phosphoribosyl transferase (Nampt)/pre-B cell colony-enhancing factor (PBEF)/visfatin is a protein displaying multiple functional properties. Originally described as a cytokine-like protein able to regulate B cell development, apoptosis, and glucose metabolism, this protein also plays an important role in NAD biosynthesis. To gain insight into its physiological role, we have generated a mouse strain expressing a conditional Nampt allele. Lack of Nampt expression strongly affects development of both T and B lymphocytes. Analysis of hemizygous cells and in vitro cell lines expressing distinct levels of Nampt illustrates the critical role of this protein in regulating intracellular NAD levels. Consequently, a clear relationship was found between intracellular Nampt levels and cell death in response to the genotoxic agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine), confirming that this enzyme represents a key regulator of cell sensitivity to NAD-consuming stress secondary to poly(ADP-ribose) polymerases overactivation. By using mutant forms of this protein and a well-characterized pharmacological inhibitor (FK866), we unequivocally demonstrate that the ability of the Nampt to regulate cell viability during genotoxic stress requires its enzymatic activity. Collectively, these data demonstrate that Nampt participates in cellular resistance to genotoxic/oxidative stress, and it may confer to cells of the immune system the ability to survive during stressful situations such as inflammation. [less ▲]

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See detailInfluence of different exposure conditions to cigarette smoke on the pulmonary acute inflammatory response in mice
Steuve, J.; Cheu, Esteban ULg; Fievez, Laurence ULg et al

in Acta Physiologica (2008), 192(662), 18

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See detailExpression génique et médecine vétérinaire
Ramery, Eve ULg; Van Erck, Emmanuelle ULg; Bureau, Fabrice ULg et al

in Proceedings des 36èmes Journées AVEF (2008)

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See detailUse of a human microarray to highlight new genes of interest for a better understanding of recurrent airway obstruction in horses (heaves)
Ramery, Eve ULg; Closset, R.; Bureau, Fabrice ULg et al

in XVIth International Plant & Animal Genome Conference (2008)

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See detailAnti-inflammatory effects of ipratropium bromide in rats with cadmium-induced acute pulmonary inflammation
Zhang, Yinghong ULg; Fievez, Laurence ULg; Cheu, Esteban ULg et al

in Fundamental & Clinical Pharmacology (2008), 22(1), 7

Detailed reference viewed: 43 (3 ULg)