References of "Bureau, Fabrice"
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See detailInterstitial macrophages are essential for maintaining immune homeostasis in the lung
Bedoret, Denis; Wallemacq, Hugues ULg; Marichal, Thomas ULg et al

in Proceedings of The Allergy & Asthma Symposium: Bridging Innate and Adaptive Immunity (2009)

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See detailLung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice
Bedoret, Denis ULg; Wallemacq, Hugues ULg; Marichal, Thomas ULg et al

in Journal of Clinical Investigation (2009), 119(12), 3723-38

The respiratory tract is continuously exposed to both innocuous airborne antigens and immunostimulatory molecules of microbial origin, such as LPS. At low concentrations, airborne LPS can induce a lung DC ... [more ▼]

The respiratory tract is continuously exposed to both innocuous airborne antigens and immunostimulatory molecules of microbial origin, such as LPS. At low concentrations, airborne LPS can induce a lung DC-driven Th2 cell response to harmless inhaled antigens, thereby promoting allergic asthma. However, only a small fraction of people exposed to environmental LPS develop allergic asthma. What prevents most people from mounting a lung DC-driven Th2 response upon exposure to LPS is not understood. Here we have shown that lung interstitial macrophages (IMs), a cell population with no previously described in vivo function, prevent induction of a Th2 response in mice challenged with LPS and an experimental harmless airborne antigen. IMs, but not alveolar macrophages, were found to produce high levels of IL-10 and to inhibit LPS-induced maturation and migration of DCs loaded with the experimental harmless airborne antigen in an IL-10-dependent manner. We further demonstrated that specific in vivo elimination of IMs led to overt asthmatic reactions to innocuous airborne antigens inhaled with low doses of LPS. This study has revealed a crucial role for IMs in maintaining immune homeostasis in the respiratory tract and provides an explanation for the paradox that although airborne LPS has the ability to promote the induction of Th2 responses by lung DCs, it does not provoke airway allergy under normal conditions. [less ▲]

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See detailComparison of gene expression in canine sino-nasal aspergillosis and idiopathic lymphoplasmacytic rhinitis: a microarray study
Vanherberghen, Morgane ULg; Bureau, Fabrice ULg; Peters, IR et al

in Proceedings of the 19th Annual ECVIM-CA Congress (2009)

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See detailLocal administration of nicotinamide enhances neutrophil apoptosis and decreases mice endotoxin-induced airway inflammation
Fernandes, C. A.; Fievez, Laurence ULg; Ucakar, B. et al

in Société Belge de Sciences Pharmaceutiques (2009)

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See detailEffects of betamethasone on inflammation and emphysema induced by cadmium nebulisation in rats
Fievez, Laurence ULg; Kirschvink, N.; Zhang, Wenhui H. et al

in European Journal of Pharmacology (2009), 606(1-3), 210-214

Cadmium (Cd) induces centrilobular emphysema and is suspected to contribute to tobacco related lung diseases as chronic obstructive pulmonary disease (COPD). This study aimed to check whether the ... [more ▼]

Cadmium (Cd) induces centrilobular emphysema and is suspected to contribute to tobacco related lung diseases as chronic obstructive pulmonary disease (COPD). This study aimed to check whether the inflammatory process observed in rats exposed to nebulised Cd is resistant to betamethasone as observed in COPD and to determine the influence of this drug on airspace enlargement together with the MMP-2-9/TIMP-1-2 imbalance. Our results showed that betamethasone induced emphysema by itself in healthy rats. Moreover, pre-treatment of rats with betamethasone could only partially modulate the increase in bronchoalveolar lavage fluid cell counts and the absence of preventive effect of this compound against emphysema development is associated with its inability to rebalance the MMP-2-9/TIMP-1-2 ratio. [less ▲]

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See detailExpression microarrays in equine sciences
Ramery, Eve ULg; Closset, Rodrigue; Art, Tatiana ULg et al

in Veterinary Immunology and Immunopathology (2009), 127(3-4), 197-202

Microarrays have become an important research tool for life science researchers. Expression microarrays are capable of profiling the gene expression pattern of tens of thousands of genes in a single ... [more ▼]

Microarrays have become an important research tool for life science researchers. Expression microarrays are capable of profiling the gene expression pattern of tens of thousands of genes in a single experiment. It appears to be the platform of choice for parallel gene expression profiling. Various equine-specific gene expression microarrays have been generated and used. However, homologous microarrays are not yet commercially available for the horse. An alternative is the use of heterologous microarrays, mainly microarrays specific for mice or humans. Although the use of microarrays in equine research is still in its infancy, gene expression microarrays have shown their potential in equine research. This review presents the previous, current and potential use of expression microarrays in equine research. [less ▲]

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See detailInterleukin-6/STAT3 signaling regulates the ability of naive T cells to acquire B-cell help capacities
Eddahri, Fouad; Denanglaire, Sebastien; Bureau, Fabrice ULg et al

in Blood (2009), 113(11), 2426-2433

The conditions leading to the activation/differentiation of T-helper (Th) cells dedicated for B-cell antibody production are still poorly characterized. We now demonstrate that interleukin-6 (IL-6 ... [more ▼]

The conditions leading to the activation/differentiation of T-helper (Th) cells dedicated for B-cell antibody production are still poorly characterized. We now demonstrate that interleukin-6 (IL-6) promotes the differentiation of naive T lymphocytes into helper cells able to promote B-cell activation and antibody secretion. IL-6-driven acquisition of B-cell help capacity requires expression of the signal transducer and activator of transcription 3 (STAT3), but not STAT4 or STAT6 transcription factors, suggesting that the ability to provide help to B cells is not restricted to a well-defined Th1 or Th2 effector population. T cell-specific STAT3-deficient mice displayed reduced humoral responses in vivo that could not be related to an altered expansion of CXCR5-expressing helper T cells. IL-6 was shown to promote IL-21 secretion, a cytokine that was similarly found to promote the differentiation of naive T cells into potent B-cell helper cells. Collectively, these data indicate that the ability to provide B-cell help is regulated by IL-6/IL-21 through STAT3 activation, independently of Th1, Th2, Th17, or follicular helper T cell (T(FH)) differentiation [less ▲]

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See detailPTX3: a new marker for local inflammation ?
Ramery, Eve ULg; Bureau, Fabrice ULg; Art, Tatiana ULg et al

in Proceedings of the 11th ECVCP congress, Thessaloniki, Grece (2009)

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See detailDust exposure exacerbates the expression of ptx3, a recently discovered acute phase protein, in horses’ airways
Ramery, Eve ULg; Bureau, Fabrice ULg; Lekeux, Pierre ULg

Conference (2009)

Heaves is a common cause of recurrent airway obstruction (R.A.O) that occurs in mature horses stabled in a dusty environment. The long pentraxin PTX3 play an important role in the host defence and over ... [more ▼]

Heaves is a common cause of recurrent airway obstruction (R.A.O) that occurs in mature horses stabled in a dusty environment. The long pentraxin PTX3 play an important role in the host defence and over-expression of PTX3 may contribute to airways injury. Therefore, we hypothesised that PTX3 may have relevance in the understanding of the pathogenesis of recurrent airway obstruction (R.A.O). In the present study, we first investigated the equine PTX3 (ePTX3) structure by cloning, sequencing and western-blotting. A 120pb deletion was found in the second exon of ePTX3 in comparison to human PTX3. Moreover, an alternative splicing occurs with complete deletion of the second exon, resulting in two forms of the protein, “spliced” (32 kD) and “full length” (42 kD). The pentraxin domain is very well conserved in the two forms with 94% of amino-acids conserved between equines and humans. These findings could have implications for the comprehension of the function of each domain of the protein. Then, we studied the effects of dust exposure and subsequent R.A.O crisis on ePTX3 expression in the broncho-alveolar lavage fluid (BALF) and the bronchial epithelial cells. PTX3 could be detected in BALF macrophages, neutrophils, eosinophils and bronchial epithelial cells. It was over-expressed in the BALF supernatant from RAO-affected horses in crisis. Dust exposure induced PTX3 in BALF macrophages either in healthy horses or in R.A.O-affected horses. Conversely, PTX3 was strongly over-expressed in the bronchial epithelial cells from R.A.O-affected horses in crisis. Considering epithelial cells as one of the major cell types in the airways, PTX3 produced at this level may play an important role in inflammatory process. Moreover, it suggests that dust-induced expression of PTX3 is differentially regulated in macrophages and bronchial-epithelial cells. A JNK-dependant, NFκB-independent regulatory pathway has been described in lung epithelial cells. A comparable mechanism could be involved in bronchial epithelial cells. If this is confirmed, PTX3 expression in respiratory epithelial cells could be therapeutically targeted. [less ▲]

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See detailExpression génique et médecine vétérinaire
Ramery, Eve ULg; Van Erck, Emmanuelle ULg; Bureau, Fabrice ULg et al

in Proceedings des 36èmes Journées AVEF (2008)

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See detailHow transcriptomic studies may help to improve the control of bovine diseases : an example with calf pneumonia and endotoxemia
Wallemacq, Hugues ULg; Ramery, Eve ULg; Bureau, Fabrice ULg et al

in Proceedings: XXVth Jubilee World Buiatrics Congress (2008)

Global change in genes expression induced by pathological processes can now be analysed in cattle by new tools called microarrays. These transcriptomic studies may help to better understand the ... [more ▼]

Global change in genes expression induced by pathological processes can now be analysed in cattle by new tools called microarrays. These transcriptomic studies may help to better understand the pathophysiological mechanisms responsible for the disease and may therefore contribute to develop more efficient preventive and curative strategies. An example is given with a model of calf pneumonia and endotoxemia [less ▲]

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See detailLung interstitial macrophages prevent lipopolysaccharide-triggered T helper type 2 responses to harmless inhaled antigens
Bedoret, D.; Wallemacq, Hugues ULg; Marichal, Thomas ULg et al

in Proceedings of the Annual BIS-meeting (2008)

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See detailIr-LBP, an ixodes ricinus tick salivary LTB4-binding lipocalin, interferes with host neutrophil function.
Beaufays, Jérôme ULg; Adam, Benoit; Menten-Dedoyart, Catherine ULg et al

in PLoS ONE (2008), 3(12), 3987

BACKGROUND: During their blood meal, ticks secrete a wide variety of proteins that can interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation ... [more ▼]

BACKGROUND: During their blood meal, ticks secrete a wide variety of proteins that can interfere with their host's defense mechanisms. Among these proteins, lipocalins play a major role in the modulation of the inflammatory response. METHODOLOGY/PRINCIPAL FINDINGS: We previously identified 14 new lipocalin genes in the tick Ixodes ricinus. One of them codes for a protein that specifically binds leukotriene B4 with a very high affinity (Kd: +/-1 nM), similar to that of the neutrophil transmembrane receptor BLT1. By in silico approaches, we modeled the 3D structure of the protein and the binding of LTB4 into the ligand pocket. This protein, called Ir-LBP, inhibits neutrophil chemotaxis in vitro and delays LTB4-induced apoptosis. Ir-LBP also inhibits the host inflammatory response in vivo by decreasing the number and activation of neutrophils located at the tick bite site. Thus, Ir-LBP participates in the tick's ability to interfere with proper neutrophil function in inflammation. CONCLUSIONS/SIGNIFICANCE: These elements suggest that Ir-LBP is a "scavenger" of LTB4, which, in combination with other factors, such as histamine-binding proteins or proteins inhibiting the classical or alternative complement pathways, permits the tick to properly manage its blood meal. Moreover, with regard to its properties, Ir-LBP could possibly be used as a therapeutic tool for illnesses associated with an increased LTB4 production. [less ▲]

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See detailUsing a human microarray to highlight new genes of interest for a better understanding of molecular mechanisms that underpin the physiopathology of heaves
Ramery, Eve ULg; Closset, Rodrigue; Bureau, Fabrice ULg et al

in Proceedings: Plant and Animal Genome Conference, Equine Workshop, San Diego (2008)

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Using reverse transcription polymerase chain reaction (RT-PCR), disparate results have been ... [more ▼]

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Using reverse transcription polymerase chain reaction (RT-PCR), disparate results have been obtained concerning cytokines expression profile. cDNA microarray appears to be so far the platform of choice for massively parallel gene expression profiling and provides a good tool for exploratory research. However, equine-specific microarrays are not yet available on the market. Because they are commercially available, highly specific and well annotated, human and mouse large-scale microarrays are an exploratory alternative to equine-specific microarrays. In the present study, the purpose was to highlight new targets not previously related to the disease and able to improve our understanding of the molecular mechanisms of the disease. A human microarray was used to study gene expression in nucleated cells originating from peripheral blood and bronchoalveolar lavage fluid in heaves-affected horses. With a four-fold cut-off, a total of 46 candidates were identified with differentially regulated genes between heaves-affected horses and controls. Based on their documented function, five of these genes were selected for the real-time quantitative RT-PCR (RT-qPCR) validation procedure: CYBB, BTG1, MARCKS, PTX3 and PTPRC. The RT-qPCR results confirmed those obtained with the microarray, pointing out these genes as new directions for future experiments. However, the human microarray failed to detect the presence of IL-1beta and Il-8, otherwise confirmed by RT-qPCR, and the expression profile of the disease could not be obtained [less ▲]

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See detailAnti-inflammatory effects of ipratropium bromide in rats with cadmium-induced acute pulmonary inflammation
Zhang, W.; Fievez, Laurence ULg; Cheu, Esteban ULg et al

in Proceedings of the 12th Annual Meeting of the French Society of Pharmacology and Therapeutics (2008)

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See detailRole of beta 2-receptors in the anti-inflammatory effects of formoterol in rats with cadmium-induced acute pulmonary inflammation
Zhang, W.; Fievez, Laurence ULg; Cheu, Esteban ULg et al

in Fundamental & Clinical Pharmacology (2008), 227

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See detailHunting for a key to the enigma of heaves in the black box of the white cells
Art, Tatiana ULg; Bureau, Fabrice ULg; Robinson, N Edward

in Veterinary Journal (2008), 177(3), 307-308

Detailed reference viewed: 56 (21 ULg)