References of "Bureau, Fabrice"
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See detailAdaptation to multiday ozone exposure is associated with a sustained increase of bronchoalveolar uric acid.
Kirschvink, Nathalie; Fievez, Laurence ULg; Bureau, Fabrice ULg et al

in Free Radical Research (2002), 36(1), 23-32

The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant ... [more ▼]

The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant defence. Six calves were exposed to 0.75 ppm ozone, 12 h day(-1) for seven consecutive days. Pulmonary function tests and bronchoalveolar lavage (BAL) were performed before, after the first (D1), third (D3) and seventh (D7) exposure. Differential cell count, total proteins, 8-epi-PGF2alpha, glutathione and uric acid were determined in BAL. Dynamic lung compliance and arterial oxygen tension were significantly decreased and lung oedema impaired pulmonary function on D1. By repeating ozone exposures, progressive functional adaptation occurred. Ozone induced a significant increase of BAL neutrophil percentage on D1. On D3 and D7, neutrophil percentage was progressively decreased, but remained significantly elevated. BAL total proteins were significantly increased on D1 and decreased progressively until D7. 8-Epi-PGF2alpha was significantly increased on D1 and was returned to baseline on D3 and D7, whilst glutathione significantly increased on D3 and returned to baseline on D7. Uric acid was increased ten-fold on D1. On D3, uric acid was increased six-fold and was persistently elevated at D7. This study suggests that ozone adaptation of functional and inflammatory variables is accompanied with sustained BAL uric acid elevation. [less ▲]

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See detailCD40 engagement enhances eosinophil survival through induction of cellular inhibitor of apoptosis protein 2 expression: Possible involvement in allergic inflammation.
Bureau, Fabrice ULg; Seumois, Gregory; Jaspar, Fabrice et al

in Journal of Allergy and Clinical Immunology (The) (2002), 110(3), 443-9

BACKGROUND: CD40 engagement enhances eosinophil survival, suggesting a role for this receptor in the development of eosinophilia. OBJECTIVE: We examined whether CD40 enhances eosinophil survival by ... [more ▼]

BACKGROUND: CD40 engagement enhances eosinophil survival, suggesting a role for this receptor in the development of eosinophilia. OBJECTIVE: We examined whether CD40 enhances eosinophil survival by inducing the expression of antiapoptotic proteins. Three members of the inhibitor of apoptosis protein (IAP) family, namely cellular (c)-IAP1, c-IAP2, and XIAP, and 2 antiapoptotic proteins of the Bcl-2 family, namely Bcl-x(L) and Bfl-1/A1, were investigated. METHODS: Blood and sputum were obtained from healthy subjects and atopic asthmatic patients. Blood eosinophils were isolated by means of magnetic selection. Expression of CD40, IAPs, and Bcl-2 proteins was investigated by using flow cytometry, immunoblotting, or both. CD40 stimulation was achieved with agonistic antibodies or soluble ligands. Apoptosis was assessed by staining with propidium iodide and FITC-conjugated annexin-V. c-IAP2 expression was inhibited with antisense oligonucleotides. RESULTS: Freshly isolated eosinophils from healthy and asthmatic patients did not express CD40. Conversely, eosinophils expressed CD40 spontaneously when cultured for 48 hours. At this time point, CD40 stimulation significantly delayed eosinophil apoptosis. Inhibition of eosinophil apoptosis was accompanied by induction of c-IAP2 but not c-IAP1, XIAP, Bcl-x(L), or Bfl-1/A1 expression. Antisense knockdown of c-iap2 abolished CD40-induced enhancement of eosinophil survival. Sputum cells from asthmatic patients, unlike those from healthy subjects, substantially expressed CD40 and c-IAP2. Moreover, a strong correlation was found between the percentage of eosinophils in the sputum from asthmatic patients and the sputum level of CD40 and c-IAP2 expression. CONCLUSION: The results demonstrate that CD40 engagement enhances eosinophil survival through induction of c-IAP2 expression and suggest a role for this mechanism in allergic inflammation. [less ▲]

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See detailÉ possibile selezionare dei bovini meno sensibili alle malattie respiratorie ?
Lekeux, Pierre ULg; Bureau, Fabrice ULg; Jacqmot, Olivier

in Proceedinds: 4° Congresso Nazionale Multisala SIVAR (2002)

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See detailA pro-inflammatory role for the cyclopentenone prostaglandins at low concentrations: oxidative stress-induced ERK activation without NFKB inhibition
Bureau, Fabrice ULg; Desmet, Christophe ULg; Melotte, D. et al

in Proceedings : Congress "Cell signaling, transcription and translation as therapeutics targets" (2002)

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See detailNF-kB activity, and IL-8 and GM-CSF expression, in the milk of chronic mastitis-affected cows
Boulanger, D.; Bureau, Fabrice ULg; Lekeux, Pierre ULg

in Proceedings : National Mastitis Council, 41st Annual Meeting (2002)

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See detailLocal administration of nuclear factor-kB decoy oligodeoxinucleotides prevents allergic airway inflammation
Bureau, Fabrice ULg; Gosset, P.; Desmet, Christophe ULg et al

in 12th European Respiratory Society Annual Congress (2002)

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See detailImpaired Accumulation of Granulocytes in the Lung During Ozone Adaptation
Fievez, Laurence ULg; Kirschvink, N.; Dogne, S. et al

in Free Radical Biology & Medicine (2001), 31(5), 633-641

Respiratory alterations induced by an acute exposure to ozone (O(3)) paradoxically resolve during multiday exposure. This adaptation is characteristically accompanied by a gradual attenuation of lung ... [more ▼]

Respiratory alterations induced by an acute exposure to ozone (O(3)) paradoxically resolve during multiday exposure. This adaptation is characteristically accompanied by a gradual attenuation of lung neutrophilia. As maintenance of neutrophilia at the site of inflammation is due to cytokine-mediated delayed neutrophil apoptosis, which is associated with reduced levels of Bax, a proapoptotic protein, we sought to determine whether defects in these mechanisms could account for O(3) adaptation. Lung granulocytes obtained at different time points from calves exposed to 0.75 ppm O(3) for 12 h/d for 7 consecutive days neither showed enhancement of survival nor Bax deficiency, when compared to blood granulocytes. To further investigate the effects of an exogenous oxidative stress on neutrophil survival, human granulocytes were treated with hydrogen peroxide alone, or in combination with granulocyte/macrophage colony-stimulating factor, an antiapoptotic cytokine. Both treatments led to rapid apoptosis associated with downregulation of Bcl-x(L) and Bcl-2, two antiapoptotic proteins. This study shows that O(3) adaptation is associated with a failure in the mechanisms leading to accumulation of neutrophils at the site of inflammation, and suggests that this defect is due to direct proapoptotic effects of exogenous oxidative stress on granulocytes [less ▲]

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See detailP65 Homodimer Activity in Distal Airway Cells Determines Lung Dysfunction in Equine Heaves
Sandersen, Charlotte ULg; Bureau, Fabrice ULg; Turlej, R. et al

in Veterinary Immunology and Immunopathology (2001), 80(3-4), 315-26

Nuclear factor-kappaB (NF-kappaB) activity, which is a key regulator of inflammatory gene expression, is increased in bronchial epithelial cells from horses suffering from heaves (a hypersensitivity ... [more ▼]

Nuclear factor-kappaB (NF-kappaB) activity, which is a key regulator of inflammatory gene expression, is increased in bronchial epithelial cells from horses suffering from heaves (a hypersensitivity-associated inflammatory condition of the lung). To determine whether this increased activity extends to distal airways and to other pulmonary cells, cells recovered by broncho-alveolar lavage (BAL) in healthy and heaves-affected horses were assessed for NF-kappaB activity. NF-kappaB activity was much higher in BAL cells from heaves-affected horses, especially during crisis (disease exacerbation), than in cells from healthy horses. Moreover, the level of NF-kappaB activity found in BAL cells was positively correlated to total lung resistance and to the proportion of neutrophils present in BAL fluid. Finally, prototypical p65-p50 NF-kappaB heterodimers were absent from BAL cells, which mostly contained p65 homodimers. These results (1) show that increased NF-kappaB activity is a general feature of heaves lung; (2) demonstrate the importance of p65 homodimers in neutrophilic inflammation; and (3) suggest that the use of specific NF-kappaB inhibitors could improve lung function in heaves-affected horses. [less ▲]

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See detailExpression of c-inhibitor of apoptosis protein-2 in the sputum from asthmatic patients
Bureau, Fabrice ULg; Seumois, G.; Jaspar, F. et al

in Proceedings : 11th European Respiratory Society Annual Congress, Eur. Respir. J., 2001, 18 (Suppl. 33) (2001)

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See detailComparison of inulin with urea as dilutional markers of bronchoalveolar lavage in healthy and heaves-affected horses.
Kirschvink, Nathalie; Fievez, Laurence ULg; Dogne, S. et al

in Veterinary Research (2001), 32(2), 145-154

Solute analysis in bronchoalveolar lavage fluid involves the use of dilutional markers to correct for variable recovery of pulmonary epithelial lining fluid (PELF). Urea is the best characterised ... [more ▼]

Solute analysis in bronchoalveolar lavage fluid involves the use of dilutional markers to correct for variable recovery of pulmonary epithelial lining fluid (PELF). Urea is the best characterised endogenous marker, whereas inulin appears to meet the requirements of an exogenous marker. In horses, the use of inulin has never been investigated and the impact of lower airway diseases such as heaves, on PELF recovery is unknown. In this study, five healthy and five heaves-affected horses underwent airway endoscopy and bronchoalveolar lavage. PELF recovery from bronchoalveolar lavage was calculated by the inulin and the urea method. The inulin method was compared to the urea method and differences between healthy and heaves-affected horses were analysed. From a technical and analytical point of view, inulin fulfilled the requirements of a marker of dilution as well as urea. When both healthy and heaves-affected horses groups were pooled together, PELF recovery calculated by the inulin method was significantly higher than by the urea method (6.43+/-4.08% versus 0.789+/-0.299%, P < 0.005). No significant differences were observed between healthy and heaves-affected horses, neither by the inulin nor by the urea method. Inulin did not present major advantages over urea, but the combined use of both markers can improve the standardisation of studies comparing PELF compounds, by providing upper limits (inulin dilution) and lower limits (urea dilution) of PELF recovery. [less ▲]

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See detailBronchoconstrictive properties of inhaled 8-epi-Pgf2alpha in healthy and heaves-susceptible horses
Kirschvink, Nathalie; Bureau, Fabrice ULg; Art, Tatiana ULg et al

in Veterinary Research (2001), 32(5, Sep-Oct), 397-407

The 8-epi-PGF2α is a marker of oxidative stress which is increased in lungs of asthmatic humans and heaves-susceptible horses. 8-Epi-PGF2α has also been demonstrated to be an in vitro and in vivo ... [more ▼]

The 8-epi-PGF2α is a marker of oxidative stress which is increased in lungs of asthmatic humans and heaves-susceptible horses. 8-Epi-PGF2α has also been demonstrated to be an in vitro and in vivo bronchoconstrictor in humans and rodents. We hypothesised that inhaled 8-epi-PGF2α was a bronchoconstrictor in healthy and heaves-susceptible horses in clinical remission. The effect on ventilatory mechanics of nebulised 8-epi-PGF2α was compared to that of PGF2α and U46619, a thromboxane A2 agonist. Pulmonary resistance (RL) and dynamic compliance (Cdyn) were assessed in six healthy horses and in six heaves-susceptible horses in clinical remission before (baseline) and immediately after a single inhalation challenge of 1 mg 8-epi-PGF2α, PGF2α, or U46619 and placebo. RL and Cdyn were unchanged after inhalation of 8-epi-PGF2α in healthy horses. In heaves-susceptible horses, 8-epi-PGF2α induced a significant increase of RL and a significant decrease of Cdyn when compared to baseline values. Differences between RL and Cdyn values after 8-epi-PGF2α inhalation and those of placebo inhalation were not significant. Differences with healthy horses were not significant. PGF2α and U46619 induced a significant bronchoconstriction in healthy (RL and Cdyn, versus baseline) and heaves-susceptible horses (RL and Cdyn, versus baseline and placebo). The RL increase in heaves-susceptible horses after PGF2α inhalation was significantly higher than that in healthy horses. Our results suggest that 8-epi-PGF2α is not a bronchoconstrictor in healthy horses, and a bronchoconstrictor far less efficient than PGF2α and U46619 at the same dose in heaves-susceptible horses [less ▲]

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See detailEffect of preventive antioxidant supplementation on lung function, airway inflammation and pulmonary 8-epi-PGF2 of calves exposed to ozone
Kirschvink, Nathalie; Fievez, Laurence ULg; Jacqmot, O. et al

in Proceedings: Réunion de la Société Belge de Physiologie et de Pharmacologie Fondamentales et Cliniques (2001)

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See detailAntioxidant vitamin cocktail does not improve calves'lung function and airway inflammation in ozone-induced pulmonary disease
Kirschvink, Nathalie; Jacqmot, Olivier ULg; Fievez, Laurence ULg et al

in Pflügers Archiv : European Journal of Physiology (2001), 442(R42),

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See detailEnhanced survival of lung granulocytes in an animal model of asthma: evidence for a role of GM-CSF activated STAT5 signalling pathway
Turlej, Renata K; Fievez, Laurence ULg; Sandersen, Charlotte ULg et al

in Thorax (2001), 56(9), 696-702

As granulocyte/macrophage colony stimulating factor (GM-CSF) mediated delay of granulocyte apoptosis contributes to the accumulation of inflammatory cells at the site of inflammation in many diseases, we ... [more ▼]

As granulocyte/macrophage colony stimulating factor (GM-CSF) mediated delay of granulocyte apoptosis contributes to the accumulation of inflammatory cells at the site of inflammation in many diseases, we sought to determine whether asthma is also associated with a GM-CSF dependent increase in lung granulocyte survival. Moreover, because GM-CSF mediates its effects through activation of signal transducer and activator of transcription 5 (STAT5), we also investigated the potential role of STAT5 in allergic inflammation. METHODS: Blood granulocytes were recovered from six healthy and six heaves affected horses, a model of asthma. Lung granulocytes were obtained by bronchoalveolar lavage (BAL) from the same horses. Granulocytes were cultured in the presence or absence of anti-GM-CSF receptor antibodies for different times and apoptosis was determined using the Annexin-V/propidium iodide detection method. Nuclear protein extracts from cultured granulocytes were analysed for STAT5 binding activity by electrophoretic mobility shift assay. RESULTS: BAL fluid granulocytes from heaves affected horses demonstrated a significant delay in apoptosis compared with blood granulocytes from the same horses and blood and BAL fluid granulocytes from healthy horses. Conversely, the rate of apoptosis in blood granulocytes from healthy and heaves affected horses was comparable. The enhanced survival of BAL fluid granulocytes from affected horses was suppressed in the presence of antibodies directed against GM-CSF receptors. Increased levels of active STAT5 were found in BAL fluid granulocytes from heaves affected horses and were markedly reduced after treatment with anti-GM-CSF receptor antibodies. CONCLUSIONS: These data indicate that granulocyte survival is enhanced in the lung of heaves affected horses and suggest a role for a GM-CSF activated STAT5 pathway in delaying apoptosis of lung granulocytes in this model of asthma. [less ▲]

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See detailMécanismes de la persistance de l’inflammation chez les chevaux poussifs en rémission
Bureau, Fabrice ULg; Fievez, Laurence ULg; Kirschvink, Nathalie et al

in Proceedings: 27ème Journée de la Recherche Equine (2001)

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See detailTranscription factors in asthma
Bureau, Fabrice ULg

in Proceedings: Basic and Clinical Allergy Meeting (2001)

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See detailEnhanced survival of lung granulocytes in an animal model of asthma : evidence for a role of GM-CSF-activated STAT5 signaling pathway
Turlej, R.; Sandersen, Charlotte ULg; Fievez, Laurence ULg et al

in Proceedings: International Conference of the American Thoracic Society (2001)

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See detailComparison of inulin to urea as dilutional marker of bronchoalveolar lavage in an animal model of asthma
Kirschvink, Nathalie; Fievez, Laurence ULg; Dogné, S. et al

in Proceedings: International Conference of the American Thoracic Society (2001)

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See detailAdaptation to multiday ozone exposure is associated with a failure in granulocytes apoptosis delay
Fievez, Laurence ULg; Kirschvink, Nathalie; Lekeux, Pierre ULg et al

in Proceedings: International Conference of the American Thoracic Society (2001)

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