References of "Bureau, Fabrice"
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See detailNouvelles approches du traitement des affections respiratoires du bétail
Lekeux, Pierre ULg; Bureau, Fabrice ULg

in Point Vétérinaire (2005), 252

Devant des affections surtout polyfactorielles, l’ère du “tout antibiotique” semble révolue, comme l’illustrent certaines pistes actuelles de recherche.

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See detailSelective decoy-mediated inhibition of activator protein-1 activity in the airways pevents experimental asthmatic inflammation
Desmet, Christophe ULg; Gosset, P.; Pajak, B. et al

in European Respiratory Journal (2005), 26

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See detailGSK3-Mediated BCL-3 phosphorylation modulates its degradation and its oncogenicity
Viatour, Patrick ULg; Dejardin, Emmanuel ULg; Warnier, Michael et al

in Molecular Cell (2004), 16(1), 35-45

The oncoprotein BCL-3 is a nuclear transcription factor that activates NF-kappaB target genes through formation of heterocomplexes with p50 or p52. BCL-3 is phosphorylated in vivo, but specific BCL-3 ... [more ▼]

The oncoprotein BCL-3 is a nuclear transcription factor that activates NF-kappaB target genes through formation of heterocomplexes with p50 or p52. BCL-3 is phosphorylated in vivo, but specific BCL-3 kinases have not been identified so far. In this report, we show that BCL-3 is a substrate for the protein kinase GSK3 and that GSK3-mediated BCL-3 phosphorylation, which is inhibited by Akt activation, targets its degradation through the proteasome pathway. This phosphorylation modulates its association with HDAC1, -3, and -6 and attenuates its oncogenicity by selectively controlling the expression of a subset of newly identified target genes such as SLPI and CxcI1. Our results therefore suggest that constitutive BCL-3 phosphorylation by GSK3 regulates BCL-3 turnover and transcriptional activity. [less ▲]

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See detailCeramides play a critical role in spontaneous neutrophil apoptosis
Seumois, G.; Fillet, Marianne ULg; Gillet, Laurent ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailActivator protein-1 activity in bronchial brushing samples from horses with recurrent airway obstruction
Couëtil, L.; Art, Tatiana ULg; Bureau, Fabrice ULg et al

in Proceedings of the 22nd Symposium of the Veterinary and Comparative Respiratory Society (2004)

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See detailNuclear factor kappaB inhibition in bovine mammary epithelial cells reduces intracellular infection by Staphylococcus aureus.
Boulanger, D.; Bureau, Fabrice ULg; Lekeux, Pierre ULg

in Proceedings : 23rd World Buiatrics Congress (2004)

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See detailSTAT5 promotes granulocyte survival during inflammation
Fievez, Laurence ULg; Desmet, Christophe ULg; Pajak, B. et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailEffects of thiazolidinediones on tumor necrosis factor R alpha induced inflammatory cytokine expression
Desmet, Christophe ULg; Warsée, Barbara; Mélotte, D. et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailEvidence for a role of heat shock factor 1 in inhibition of NF-kB pathway during heat shock response-mediated lung protection
Wirth, D.; Bureau, Fabrice ULg; Melotte, D. et al

in American Journal of Physiology - Lung Cellular and Molecular Physiology (2004), 287

Heat shock transcription factor (HSF)-1 is recognized as a central component of the heat shock response, which protects against various harmful conditions. However, the mechanisms underlying the ... [more ▼]

Heat shock transcription factor (HSF)-1 is recognized as a central component of the heat shock response, which protects against various harmful conditions. However, the mechanisms underlying the protection and the role of HSF-1 in these mechanisms have not yet been clearly elucidated. Using HSF-1 knockout mice (Hsf1_/_), we examined whether heat shock responsemediated lung protection involved an inhibition of the proinflammatory pathway via an interaction between HSF-1 and NF-_B, in response to cadmium insult. The HSF-1-dependent protective effect against intranasal instillation of cadmium (10 and 100 _g/mouse) was demonstrated by the higher protein content (1.2- and 1.4-fold), macrophage (1.6- and 1.9-fold), and neutrophil (2.6- and 1.8-fold) number in bronchoalveolar fluids, higher lung wet-to-dry weight ratio, and more severe lung damage evaluated by histopathology in Hsf1_/_compared with wild-type animals. These responses were associated with higher granulocyte/macrophage colony-stimulating factor (GMCSF; 1.7-fold) but not TNF-_ concentrations in bronchoalveolar fluids of Hsf1_/_ mice compared with those of wild-type animals, indicating that HSF-1 behaved as a repressor of specific cytokine production in our model. To further investigate the mechanism of GM-CSF repression, we analyzed the NF-_B activity and I_B stability. The DNA binding NF-_B activity, in particular p50 homodimer activity, was higher in Hsf1_/_ mice than in wild-type mice after cadmium exposure. These results provide a first line of evidence that mechanisms of lung protection depending on HSF-1 involve specific cytokine repression via inhibition of NF-_B activation in vivo. [less ▲]

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See detailChronic mastitis-affected cows display lower lipoxin levels than acute mastitis-affected cows
Boutet, Philippe ULg; Bureau, Fabrice ULg; Degand, Guy ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailDelayed neutrophil apoptosis in bovine subclinical mastitis.
Boutet, Philippe ULg; Boulanger, D.; Gillet, Laurent ULg et al

in Journal of Dairy Science (2004), 87(12), 4104-4114

Bovine subclinical mastitis can be defined as a moderated inflammatory disease characterized by a persistent accumulation of neutrophils in milk. As GMCSF-mediated delay of neutrophil apoptosis ... [more ▼]

Bovine subclinical mastitis can be defined as a moderated inflammatory disease characterized by a persistent accumulation of neutrophils in milk. As GMCSF-mediated delay of neutrophil apoptosis contributes to the accumulation of inflammatory cells at the site of inflammation in many human diseases, we sought to determine whether subclinical mastitis in cows is also associated with a GMCSF-dependent increase in milk-neutrophil survival. We first addressed the hypothesis that GMCSF delays bovine neutrophil apoptosis by activation of the signal transducer and activator of transcription (STAT) family members STAT3 and STAT5, which are critical regulators of the expression of various Bcl-2 family proteins. Granulocyte-macrophage colony-stimulating factor significantly delayed apoptosis of blood neutrophils obtained from healthy cows. In these cells, GMCSF activated STAT5, but not STAT3, and induced an increase in the mRNA of the antiapoptotic Bcl-2 member, Bcl-xL. Granulocyte-macrophage colony-stimulating factor-dependent STAT5 activation and up-regulation of Bcl-xL mRNA were blocked by the Jak inhibitor, AG-490. This inhibition was associated with abrogation of the prosurvival effect of GMCSF, demonstrating a key role for STAT5 in delayed neutrophil apoptosis. We further found that GMCSF expression was increased in milk cells from cows affected with subclinical mastitis. Neutrophils from these cows demonstrated a significant delay of apoptosis as compared with neutrophils obtained from healthy cows and were unresponsive to GMCSF. Active STAT5 complexes were detected in these neutrophils. Finally, in the presence of AG-490, apoptosis was induced and a time-dependent down-regulation of Bcl-xL mRNA was observed in milk neutrophils from mastitis-affected cows. These results indicate that neutrophil survival is enhanced in milk of subclinical mastitis-affected cows and suggest a role for a GMCSF-activated STAT5 signaling pathway in this phenomenon. This pathway could thus represent a target for the control of persistent accumulation of neutrophils in the bovine mammary gland [less ▲]

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See detailSelective blockade of NF-kappa B activity in airway immune cells inhibits the effector phase of experimental asthma
Desmet, Christophe ULg; Gosset, P.; Pajak, B. et al

in Journal of Immunology (2004), 173(9), 5766-5775

Knockout mice studies have revealed that NF-kappaB plays a critical role in Th2 cell differentiation and is therefore required for induction of allergic airway inflammation. However, the questions of ... [more ▼]

Knockout mice studies have revealed that NF-kappaB plays a critical role in Th2 cell differentiation and is therefore required for induction of allergic airway inflammation. However, the questions of whether NF-kappaB also plays a role in the effector phase of airway allergy and whether inhibiting NF-kappaB could have therapeutic value in the treatment of established asthma remain unanswered. To address these issues, we have assessed in OVA-sensitized wild-type mice the effects of selectively antagonizing NF-kappaB activity in the lungs during OVA challenge. Intratracheal administration of NF-kappaB decoy oligodeoxynucleotides to OVA-sensitized mice led to efficient nuclear transfection of airway immune cells, but not constitutive lung cells and draining lymph node cells, associated with abrogation of NF-kappaB activity in the airways upon OVA provocation. NF-kappaB inhibition was associated with strong attenuation of allergic lung inflammation, airway hyperresponsiveness, and local production of mucus, IL-5, IL-13, and eotaxin. IL-4 and OVA-specific IgE and IgG1 production was not reduced. This study demonstrates for the first time that activation of NF-kappaB in local immune cells is critically involved in the effector phase of allergic airway disease and that specific NF-kappaB inhibition in the lungs has therapeutic potential in the control of pulmonary allergy [less ▲]

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See detailChronic mastitis-affected cows display lower lipoxin levels than acute mastitis-affected cows
Boutet, Philippe ULg; Bureau, Fabrice ULg; Degand, Guy ULg et al

in Recueil "Le Médecin Vétérinaire du Québec" (2004), 34(1 et 2), 160

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See detailSTAT5 promotes granulocyte survival during lung inflammation
Fievez, Laurence ULg; Desmet, Christophe ULg; Seumois, G. et al

in Proceedings: 22nd Symposium of the Veterinary and Comparative Respiratory Society (2004)

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See detailAntiapoptotic effect of GM-CSF activated STAT5 signaling pathway in chronic mastitis-affected cows : possible involvement in persistent accumulation of neutrophils in milk
Boutet, Philippe ULg; Bureau, Fabrice ULg; Gillet, Laurent ULg et al

in Congress COST Action 844: “Apoptosis and programmed cell death : molecular mechanisms and applications in Biotechnology and Agriculture” (2004)

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See detailIncreased nuclear factor kappa B activity in Milk Cells of Mastitis-Affected Cows
Boulanger, D.; Bureau, Fabrice ULg; Melotte, D. et al

in Journal of Dairy Science (2003), 86(4), 1259-1267

Bacterial mastitis is accompanied by a drastic increase in milk somatic cell count (SCC), with neutrophils being the predominant cell type found in the infected quarters. Accumulation and activation of ... [more ▼]

Bacterial mastitis is accompanied by a drastic increase in milk somatic cell count (SCC), with neutrophils being the predominant cell type found in the infected quarters. Accumulation and activation of neutrophils at the site of infection require local expression of many inflammatory genes encoding adhesion molecules, chemokines and cytokines. Most of the inflammatory genes contain binding sites for the nuclear factor kappaB (NF-kappaB) within their promoter and therefore partly depend on NF-kappaB for their expression. We thus hypothesized that an increase in NF-kappaB activity in the mammary gland could contribute to development of the neutrophilic inflammation that characterizes mastitis. In an attempt to verify this hypothesis, we first assessed milk cells from healthy and acute and chronic mastitis-affected cows for NF-kappaB activity using electrophoretic mobility shift assays. We next studied the relationships between the intensity of NF-kappaB activity in these cells and the degree of udder inflammation. Active NF-kappaB complexes were undetectable in milk cells from healthy cows, whereas high levels of NF-kappaB activity were always found in cells from cows with acute mastitis. In milk cells obtained from chronic mastitis-affected cows, NF-kappaB activity varied from low to high. Finally, the level of NF-kappaB activity measured in milk cells from chronic mastitis-affected cows was not correlated to SCC or to the proportion of neutrophils present in milk samples, but was highly correlated with the expression level of interleukin-8 and granulocyte/macrophage colony-stimulating factor, two NF-kappaB-dependent cytokines crucially involved in initiation and perpetuation of neutrophilic inflammation. These results suggest that NF-kappaB might play a role in mastitis pathogenesis. [less ▲]

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See detailCeramides play a critical role in spontaneous neutrophil apoptosis
Seumois, G.; Fillet, Marianne ULg; Gillet, Laurent ULg et al

Poster (2003)

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See detailImbalance between lipoxin A(4) and leukotriene B-4 in chronic mastitis-affected cows
Boutet, Philippe ULg; Bureau, Fabrice ULg; Degand, Guy ULg et al

in Journal of Dairy Science (2003), 86(11), 3430-3439

Persistent accumulation of inflammatory cells in the udder, with neutrophils being the predominant cell type, is a characteristic feature of chronic mastitis in dairy cows. Leukotriene (LT) B-4 is a ... [more ▼]

Persistent accumulation of inflammatory cells in the udder, with neutrophils being the predominant cell type, is a characteristic feature of chronic mastitis in dairy cows. Leukotriene (LT) B-4 is a potent chemotactic agent, known to induce recruitment and accumulation of neutrophils in the bovine mammary gland. The LTB4-stimulated neutrophil functional responses are closely opposed by lipoxin (LX) A(4), which promotes the resolution of inflammation. We thus hypothesized that the chronic inflammation of the udder could be associated with an unfavorable ratio between these two eicosanoids and that the persistence of neutrophil accumulation could be due to an increase in LTB4 synthesis and/or an impaired LXA(4) production. In an attempt to verify this hypothesis, we first measured LXA(4), LTB4, and their ratio in the milk of healthy and acute and chronic mastitis-affected quarters. Next, we studied the relationships between these variables and the degree of udder inflammation as assessed by somatic cell count measurement. The LTB4 concentration was low in healthy quarters, drastically increased in acute mastitis, and reached intermediate levels in chronic mastitis-affected quarters. However, whereas LXA(4) concentration was highly increased in acute mastitis, healthy and chronic quarters had similarly low values. The LXA(4):LTB4 ratio was thus significantly lower in chronic mastitis-affected cows. The LTB4 concentrations measured in chronic quarters were highly correlated to somatic cell count and to milk neutrophil and macrophage numbers. A weaker correlation was observed between LXA(4) and these variables. For both eicosanoids, the highest correlation was observed with the number of neutrophils. These results show the existence of an LXA(4):LTB4 imbalance in chronic mastitis-affected cows because of low LXA(4) concentrations. Further studies are needed to determine whether administration of LX or stable analogs could have therapeutic potential in the control of chronic bovine mastitis. [less ▲]

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