References of "Bureau, Fabrice"
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See detailDe novo C16- and C24-ceramide generation contributes to spontaneous neutrophil apoptosis.
Seumois, Gregory; Fillet, Marianne ULg; Gillet, Laurent ULg et al

in Journal of Leukocyte Biology (2007), 81(6), 1477-1486

Neutrophils rapidly undergo spontaneous apoptosis following their release from the bone marrow. Although central to leukocyte homeostasis, the mechanisms that regulate neutrophil apoptosis remain poorly ... [more ▼]

Neutrophils rapidly undergo spontaneous apoptosis following their release from the bone marrow. Although central to leukocyte homeostasis, the mechanisms that regulate neutrophil apoptosis remain poorly understood. We show here that apoptosis of cultured neutrophils is preceded by a substantial increase in the intracellular levels of 16 and 24 carbon atom (C(16)- and C(24))-ceramides, which are lipid second messengers of apoptosis and stress signaling. Treatment of neutrophils with fumonisin B(2), a selective inhibitor of the de novo pathway of ceramide synthesis, prevented accumulation of C(16)- and C(24)-ceramides. Moreover, fumonisin B(2) significantly reduced caspase-3, -8, and -9 activation and apoptosis in these cells. Conversely, 3-O-methylsphingomyelin and fantofarone, which are specific inhibitors of neutral and acid sphingomyelinases, respectively, neither inhibited C(16)- and C(24)-ceramide production nor decreased the apoptosis rate in neutrophils, indicating that in these cells, ceramides are not generated from membrane sphingomyelin. Further experiments showed that increasing endogenous C(16)- and C(24)-ceramide levels by using DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol and (1S,2R)-D-erythro-2-(N-myristoylamino)-1-phenyl-1-propanol, two inhibitors of ceramide metabolism, enhances caspase-3, -8, and -9 activity and increases neutrophil apoptosis. Similarly, apoptosis was induced rapidly when synthetic C(16)- and/or C(24)-ceramides were added to neutrophil cultures. Finally, GM-CSF, a cytokine that delays neutrophil apoptosis, abrogated C(16)- and C(24)-ceramide accumulation totally in cultured neutrophils, whereas Fas ligation accelerated apoptosis in these cells without affecting de novo ceramide production. We conclude that de novo generation of C(16)- and C(24)-ceramides contributes to spontaneous neutrophil apoptosis via caspase activation and that GM-CSF exerts its antiapoptotic effects on neutrophils, at least partly through inhibition of ceramide accumulation. [less ▲]

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See detailEffects of formoterol on repeated cadmium inhalation-induced lung inflammation and emphysema in rats
Zhang, W.; Fievez, Laurence ULg; Cheu, Esteban ULg et al

in Proceedings : Congrès de physiologie, de pharmacologie et de thérapeutique P2T (2007)

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See detailInvestigation of pulmonary expression of heat shock protein HSP70 in mice chronically exposed to cigarette smoke
Cheu, Esteban ULg; Steuve, J.; Fievez, Laurence ULg et al

in Proceedings: Autumn Meeting of the Belgian Society of Fundamental and Clinical Physiology and Pharmacology (2007)

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See detailInfluence of different exposure conditions to cigarette smoke on the pulmonary acute inflammatory response in mice
Steuve, J.; Cheu, Esteban ULg; Fievez, Laurence ULg et al

in Proceedings: Autumn Meeting of the Belgian Society of Fundamental and Clinical Physiology and Pharmacology (2007)

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See detailSTAT5 is an Ambivalent Regulator of Neutrophil Homeostasis
Fievez, Laurence ULg; Desmet, Christophe ULg; Henry, E. et al

Poster (2007)

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See detailDendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma
Henry, E.; Desmet, C. J.; Garzé, V. et al

Poster (2007)

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See detailRole of beta 2-receptors in the anti-inflammatory effects of formoterol in rats with cadmium-induced acute pulmonary inflammation
Zhang, W.; Zhang, F.; Fievez, Laurence ULg et al

in Proceedings: Autumn Meeting of the Belgian Society of Fundamental and Clinical Physiology and Pharmacology (2007)

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See detailCaractériser un profil inflammatoire grâce à l'utilisation du microdamier
Ramery, Eve ULg; Closset, Rodrigue; Bureau, Fabrice ULg et al

Poster (2007)

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See detailHelenalin reduces Staphylococcus aureus infection in vitro and in vivo
Boulanger, D.; Brouillette, E.; Jaspar, F. et al

in Veterinary Microbiology (2007), 119(2-4), 330-338

Staphylococcus (S.) aureus is a major udder pathogen causing bovine mastitis. Some pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-alpha), enhance extracellular and intracellular ... [more ▼]

Staphylococcus (S.) aureus is a major udder pathogen causing bovine mastitis. Some pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-alpha), enhance extracellular and intracellular growth of S. aureus, indicating that the inflammatory process favors S. aureus infection. Helenalin is a sesquiterpene lactone with potent anti-inflammatory properties. This study was designed to evaluate the effects of helenalin on S. aureus infection. First, in vitro experiments were conducted. These studies revealed that proliferation of S. aureus in bovine mammary epithelial MAC-T cells treated in the presence or absence of TNF-alpha was markedly reduced in the presence of helenalin. Secondly, in vivo effects of helenalin were investigated. Lactating mice treated in the presence or absence of helenalin were challenged by the intramammary route with S. aureus and the bacteria in the mammary glands were counted 12 h after infection. Significantly less numbers of bacteria were recovered from the infected glands of helenalin-treated mice compared with untreated mice. Moreover, histological examination of mammary tissue from helenalin-treated mice that were challenged with S. aureus indicated that helenalin is able to significantly reduce leukocyte infiltration in the mammary gland following S. aureus inoculation. Our results show that helenalin reduces S. aureus intracellular growth and experimental S. aureus infection. We conclude that helenalin may be of potential interest in the treatment of S. aureus-induced mastitis in the bovine species. [less ▲]

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See detailCysteinyl-leukotrienes contribute to sputum eosinophil chemotactic activity in asthmatics
Hemelaers, L.; Henket, Monique ULg; Sele, Jocelyne ULg et al

in Allergy (2006), 61(1), 136-139

Background: Cysteinyl-leukotrienes are lipid derived mediators involved in asthma. They are able to stimulate eosinophil chemotaxis in vitro. Induced sputum from asthmatics has been shown to contain ... [more ▼]

Background: Cysteinyl-leukotrienes are lipid derived mediators involved in asthma. They are able to stimulate eosinophil chemotaxis in vitro. Induced sputum from asthmatics has been shown to contain eosinophil chemotactic activity. The purpose of our study was to evaluate the contribution of cysteinyl-leukotrienes to sputum eosinophil chemotactic activity in asthmatics and to seek whether there might be differences between asthmatics free of inhaled corticosteroids vs those regularly receiving this treatment. Methods: Twenty-two patients (11 corticosteroid free, mean FEV1 99% predicted, 11 corticosteroid-treated, mean FEV1 77% predicted) recruited from our asthma clinic underwent a sputum induction. Sputum was processed according to standard procedure. Eosinophil chemotactic activity contained in the fluid phase was assessed using Boyden microchamber model and expressed as chemotaxis index (CI). Cysteinyl-leukotrienes were measured in sputum supernatant by ELISA and their role in sputum eosionophil chemotactic activity was evaluated by using montelukast, a selective antagonist of a cys-LT1 receptor. Results: Cysteinyl-leukotrienes were well detectable in sputum supernatants from both steroid-naive (247 +/- 42 pg/ml) and steroid-treated (228 +/- 26 pg/ml) asthmatics. Sputum eosinophil chemotactic activity was indiscriminately present in both corticosteroid-naive (CI: 2.61 +/- 0.22) and corticosteroid-treated (2.98 +/- 0.35) asthmatics. Montelukast (100 mu M) significantly inhibited the eosinophil chemotactic activity in both groups achieving a mean inhibition of 54.2 +/- 9.2% (P < 0.001) and 64.7 +/- 7.8% (P < 0.001) in steroid-naive and steroid-treated asthmatics respectively. Conclusion: Cysteinyl-leukotrienes actively participate in sputum eosinophil chemotactic activity found in asthmatics irrespective of whether they are or not under treatment with inhaled corticoids. [less ▲]

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See detailTableau clinique et mécanismes physiopathologiques des principales maladies inflammatoires chroniques des voies respiratoires du cheval
Mesnil, Claire ULg; Bureau, Fabrice ULg; Lekeux, Pierre ULg

in Annales de Médecine Vétérinaire (2006), 150(4), 227-240

The respiratory tract of the horse can be the seat of several chronic inflammatory diseases whose principal ones are the recurrent airway obstruction, the inflammatory airway disease, the summer pasture ... [more ▼]

The respiratory tract of the horse can be the seat of several chronic inflammatory diseases whose principal ones are the recurrent airway obstruction, the inflammatory airway disease, the summer pasture-associated obstructive pulmonary disease and the follicular pharyngitis. These diseases can have an important impact on horses’ health and on their sports performances. A good knowledge of their aetiologies and their physiopathological mechanisms would allow a better control of these affections. However, even if many studies were made on this subject, there are currently very few certainties as for the inflammatory mechanisms governing these diseases. This article aims to make an inventory of knowledge and deficiencies concerning these four chronic inflammatory diseases [less ▲]

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See detailInvasion intracellulaire des cellules non-phagocytaires par Staphylococcus aureus
Boulanger, Delphine; Bureau, Fabrice ULg; Lekeux, Pierre ULg

in Annales de Médecine Vétérinaire (2006), 150(1), 27-42

Intracellular invasion of Staphyloccocus aureus in nonphagocytic cells Staphylococcus aureus often causes chronic diseases. It is now believed that recurrence of these infections could be related to the ... [more ▼]

Intracellular invasion of Staphyloccocus aureus in nonphagocytic cells Staphylococcus aureus often causes chronic diseases. It is now believed that recurrence of these infections could be related to the ability of S. aureus to invade and persist within nonphagocytic cells. Adherence to eucaryotic cells is crucial for S. aureus to invade and persist within invasion and depends on interactions between bacterial fibronectin-binding proteins, fibronectin and the host cell fibronectin receptor, integrin alpha(5)beta(1). It is currently established that fibronectin acts as a bridging molecule. Penetration of S. aureus in host cells requires also activation of protein tyrosine kinases that mediate signal transduction and actin polymerization leading to cytoskeletal rearrangements. After internalization, S. aureus either remains in membrane-bound vacuoles or appears free in the cytoplasm. After bacterial proliferation, S. aureus induces host cell apoptosis or persist inside cells as small colony variants, which represent a less virulent subpopulation of S. aureus that grows slower. Although numerous in vitro and in vivo studies have demonstrated the ability of S. aureus to invade nonphagocytic cells, additional experiments have to be realized to understand the relevance of intracellular localization in vivo. [less ▲]

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See detailLa mammite bovine : de l’initiation à la résolution
Boutet, Philippe ULg; Bureau, Fabrice ULg; Lekeux, Pierre ULg

in Annales de Médecine Vétérinaire (2006), 150(1), 1-26

The bovine mastitis : from initiation to resolution The cure of a cow suffering from a bacterial mastitis relies on the balance between the eradication of the pathogen and the resolution of the ... [more ▼]

The bovine mastitis : from initiation to resolution The cure of a cow suffering from a bacterial mastitis relies on the balance between the eradication of the pathogen and the resolution of the inflammatory response, two processes that are essential to come back to a normal milk composition with low somatic cell count. The persistence of the inflammatory response, which main consequence is a reduction in milk yield, is a feature of chronic mastitis. This frequent disease depends on inappropriate host-pathogen interactions and is not yet well understood. This review resumes the main defence mechanisms of the bovine mammary gland, emphasizing the predominant roles played by the neutrophil, and brings some precisions on lipoxin implications in the resolution of inflammation. Reasons that may explain the persistence of the inflammatory reaction, a phenomenon found in the Staphylococcus aureus chronic mastitis, are also discussed. [less ▲]

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See detailNew trends in the diagnosis and treatment of recurrent inflammation in competition horses
Lekeux, Pierre ULg; Thomas, A.; Art, Tatiana ULg et al

in Pferdeheilkunde (2006)

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See detailProlactine triggers a pro-inflamatory response in bovine mammary epithelial cells
Boutet, Philippe ULg; Sulon, Joseph; Detilleux, Johann ULg et al

in Proceedings: 24th World Buiatrics Congress (2006)

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See detailEffect of Beclomethasone Dipropionate and Dexamethasone Isonicotinate on Lung Function, Bronchoalveolar Lavage Fluid Cytology, and Transcription Factor Expression in Airways of Horses with Recurrent Airway Obstruction
Couetil, L.; Art, Tatiana ULg; de Moffarts, Brieuc et al

in Journal of Veterinary Internal Medicine (2006), 20

Glucocorticoid (GC) therapy is recognized to be effective for the treatment of recurrent airway obstruction (RAO) in horses. Anti-inflammatory properties of GC are thought to be mediated by suppression of ... [more ▼]

Glucocorticoid (GC) therapy is recognized to be effective for the treatment of recurrent airway obstruction (RAO) in horses. Anti-inflammatory properties of GC are thought to be mediated by suppression of inflammatory gene expression via inhibition of transcription factors such as nuclear factor-kB (NF-kB) and activator protein-1 (AP-1). The purpose of this study was to evaluate the effect of low-dose inhaled beclomethasone dipropionate and injectable dexamethasone 21- isonicotinate on clinical signs, pulmonary function, airway cytology, and activity of NF-kB and AP-1 in bronchial cells of RAO-affected horses. Seven horses with RAO were exposed to moldy hay until they developed airway obstruction on 3 separate occasions. In a crossover design, they were then treated with a placebo (injection on day 1), inhaled beclomethasone (500 mg q12h for 10 days), or dexamethasone (0.06 mg/kg, IM on day 1) and monitored for 10 days. Pulmonary function, bronchoalveolar lavage fluid cytology, and NF-kB and AP-1 activity in bronchial brushing cells were measured before (day 1) and after treatment (day 10). Treatment with beclomethasone resulted in significantly improved pulmonary function of RAOaffected horses compared with placebo and dexamethasone treatments. However, none of the treatments had an effect on bronchoalveolar lavage fluid cytology or NF-kB and AP-1 activity. These findings reveal that, in a model of severe RAO, the benefits of low-dose inhaled beclomethasone on pulmonary function are not accompanied by a decrease in airway inflammatory cells or a suppression of transcription factors NF-kB and AP-1 DNA-binding activity. [less ▲]

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