References of "Blacher, Silvia"
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See detailAltered expression of angiogenesis and lymphangiogenesis markers in the uninvolved skin of plaque-type psoriasis
Henno, Audrey ULg; Blacher, Silvia ULg; Lambert, Charles ULg et al

in British Journal of Dermatology (2009), 160(3), 581-90

Background Vascular alterations are significant events in the pathomechanism of psoriasis. A disorder in the mechanisms regulating skin angiogenesis and lymphangiogenesis could participate in the ... [more ▼]

Background Vascular alterations are significant events in the pathomechanism of psoriasis. A disorder in the mechanisms regulating skin angiogenesis and lymphangiogenesis could participate in the pathogenesis of the disease. Objectives To quantify differences in the expression of angiogenesis and lymphangiogenesis growth factors, receptors, coreceptors as well as their antagonists in the uninvolved skin of patients with psoriasis compared with the skin of nonpsoriatic volunteers. Methods Skin biopsies were collected from the involved skin of 13 patients with untreated plaque-type psoriasis, from their nonlesional skin at distance from the lesions and from the skin of 16 healthy volunteers. The mRNA steady-state level of keratins 10, 14 and 16, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), vimentin, collagen I and IV, proliferating cell nuclear antigen, the various splice variants of vascular endothelial growth factor, VEGF-A, VEGF-C and VEGF-D, their receptors VEGFR1, VEGFR2 and VEGFR3, neuropilin (NRP)-1 and its soluble forms, NRP-2, semaphorin 3A and prox-1, was measured by reverse transcription–polymerase chain reaction. Immunohistochemistry was performed for Ki-67, von Willebrand factor and D2-40. Blood and lymphatic vessel density, area and distance from epidermis were estimated by morphological analysis coupled to an original computer-assisted method of quantification. Results Skin from healthy volunteers and nonlesional skin from patients with psoriasis displayed similar histological, morphometric and proliferative features. However, a significant overexpression of VEGFR3, the VEGF-A isoform VEGF121, soluble 12 NRP-1 and GAPDH was observed in the nonlesional psoriatic skin as compared with that of normal volunteers. Conclusions These data point to significant differences in the blood and lymphatic vascular transcriptome between the clinically normal-appearing skin of patients with psoriasis and the skin of volunteers without psoriasis. [less ▲]

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See detailContrast-enhanced coded phase-inversion harmonic sonography of knee synovitis correlates with histological vessel density: 2 automated digital quantifications
Kaiser, Marie-Joëlle ULg; Hauzeur, Jean-Philippe ULg; Blacher, Silvia ULg et al

in Journal of Rheumatology (2009), 36(7), 1391-400

OBJECTIVE: To use contrast-enhanced coded phase-inversion harmonic B-mode sonography to assess the acoustic enhancement of the synovial area of the knee; and to compare the data with the histological ... [more ▼]

OBJECTIVE: To use contrast-enhanced coded phase-inversion harmonic B-mode sonography to assess the acoustic enhancement of the synovial area of the knee; and to compare the data with the histological vessel density. METHODS: Eleven patients eligible for a knee arthroscopy were studied. Acoustic quantification was carried out by a digital image analysis program that detects the time-dependent increase [intensity (time) = k x time + C] of gray-level intensity in all the pixels of a specific region of interest (ROI) following intravenous injection of the microbubble contrast agent sulfur hexafluoride. Echo-guided synovial biopsies were carried out in the same ROI. Synovial vessel areas were quantified after Factor VIII immunostaining of synovial biopsies using an automated digital image analysis. RESULTS: Significant (p < 0.05) correlations were observed between histological vessel density and percentage of the synovial area with a k value > 0.01 (r = 0.93) and k(max) values (r = 0.79), as well as between the 2 latter parameters (r = 0.72). The histological vessel density and the 2 acoustic parameters were also significantly correlated with the logarithm of erythrocyte sedimentation rate (r = 0.77, r = 0.87, r = 0.67, respectively) and with log C-reactive protein serum concentration (r = 0.69, r = 0.83, r = 0.62, respectively). CONCLUSION: Contrast-enhanced coded phase-inversion harmonic B-mode sonography coupled with an appropriate data analysis method is a new tool to identify and quantify vessel density in knee synovitis. [less ▲]

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See detailTwo methods of random seed generation to avoid over-segmentation with stochastic watershed: application to nuclear fuel micrographs
Cativa Tolosa, Sebastian; Blacher, Silvia ULg; Denis, Alicia et al

in Journal of Microscopy (2009), 236

A stochastic version of the watershed algorithm is obtained by choosing randomly in the image the seeds from which the watershed regions are grown. The output of the procedure is a probability density ... [more ▼]

A stochastic version of the watershed algorithm is obtained by choosing randomly in the image the seeds from which the watershed regions are grown. The output of the procedure is a probability density function (PDF) corresponding to the probability that each pixel belongs to a boundary. In the present paper, two stochastic seed-generation processes are explored to avoid over-segmentation. The first is a non-uniform Poisson process, the density of which is optimized on the basis of opening granulometry. The second process positions the seeds randomly within disks centered on the maxima of a distance map. The two methods are applied to characterize the grain structure of nuclear fuel pellets. Estimators are proposed for the total edge length and grain number per unit area, LA and NA, which take advantage of the probabilistic nature of the PDF and do not require segmentation. [less ▲]

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See detailX-Ray Microtomography Studies of Tannin-Derived Organic and Carbon Foams
Tondi, G.; Blacher, Silvia ULg; Léonard, Angélique ULg et al

in Microscopy and Microanalysis (2009), 15

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See detailApplication of image analysis in food drying
Jinorose, Maturada; Devahastin, Sakamon; Blacher, Silvia ULg et al

in Ratti, C. (Ed.) Advances in Food Dehydration (2009)

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See detailWater desorption from resorcinol-formaldehyde hydrogels and adsorption in the resulting xerogels
Escalona, Ivonne; Gommes, Cédric ULg; Job, Nathalie ULg et al

in Microporous & Mesoporous Materials (2009), 117

Water desorption isotherms of resorcinol-formaldehyde (RF) hydrogels and subsequent water adsorption isotherms of the resulting xerogels are determined using the static gravimetric method, at various ... [more ▼]

Water desorption isotherms of resorcinol-formaldehyde (RF) hydrogels and subsequent water adsorption isotherms of the resulting xerogels are determined using the static gravimetric method, at various temperatures. Isotherms obtained from samples synthesized at various pH are compared. Two different mechanisms are involved in RF hydrogels water desorption. At large relative humidity the capillary tension resulting from water removal induces a macroscopic shrinkage of the gel, whereas at low humidity water is evaporated with no network deformation. These two mechanisms are analyzed using a plastic deformation model and the Guggenheim-Anderson-de Boer (GAB) model, respectively. Adsorption isotherms of RF xerogels are analyzed using the GAB model. [less ▲]

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See detailIn situ follow-up of adsorption in activated carbon beds using X-ray microtomography
Almazan, Maria del Carmen; Léonard, Angélique ULg; Toye, Dominique ULg et al

in Kaskel, S.; Llewellyn, P.; Rodriguez-Reinoso, F. (Eds.) et al Characterisation of Porous Solids VII - Proceedings of the 8th International Symposium on the Characterisation of Porous Solids (2009)

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See detailAntiangiogenic liposomal gene therapy with 16K human prolactin efficiently reduces tumor growth.
Kinet, Virginie ULg; Nguyen, Ngoc-Quynh-Nhu ULg; Sabatel, Céline ULg et al

in Cancer Letters (2009), 284(2), 222-228

Human 16K PRL (16K hPRL) is a potent inhibitor of angiogenesis both in vitro and in vivo. It has been shown to prevent tumor growth in three xenograft mouse models. Here we have used a gene transfer ... [more ▼]

Human 16K PRL (16K hPRL) is a potent inhibitor of angiogenesis both in vitro and in vivo. It has been shown to prevent tumor growth in three xenograft mouse models. Here we have used a gene transfer method based on cationic liposomes to produce 16K hPRL and demonstrate that 16K hPRL inhibits tumor growth in a subcutaneous B16F10 mouse melanoma model. Computer-assisted image analysis shows that 16K hPRL treatment results in the reduction of tumor vessel length and width, leading to a 57% reduction in average vessel size. We thus show, for the first time, that administration of the 16K hPRL gene complexed to cationic liposomes is effective to maintain antiangiogenic activities of 16K hPRL level. [less ▲]

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See detail6-Substituted 2-Oxo-2h-1-Benzopyran-3-Carboxylic Acid Derivatives in a New Approach of the Treatment of Cancer Cell Invasion and Metastasis
Kempen, I.; Hemmer, Marc ULg; Counerotte, Stéphane ULg et al

in European Journal of Medicinal Chemistry (2008), 43(12), 2735-50

Novel 6-substituted 2-oxo-2H-1-benzopyran-3-carboxylic acid derivatives were synthesized and their potency in reducing the invasive behaviour of HT 1080 fibrosarcoma cells was evaluated. Structure ... [more ▼]

Novel 6-substituted 2-oxo-2H-1-benzopyran-3-carboxylic acid derivatives were synthesized and their potency in reducing the invasive behaviour of HT 1080 fibrosarcoma cells was evaluated. Structure-activity relationships were deduced from biological results and will be used in further design of new active compounds. In particular, the acetoxymethyl substituent found at the 6-position of previously described active compounds can be replaced by an acetamidomethyl substituent without loss of potency; while the presence of an aryl ester function at the 3-position was preferred to a thioester or an amide function to induce marked biological activity. This work confirms the interest of aryl esters of 6-substituted coumarin-3-carboxylic acids as potential new anti-cancer agents. [less ▲]

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See detailDetermination of the tortuosity of porous materials from binary or grey-tone tomographic reconstructions
Gommes, Cédric ULg; Almazan, Maria del Carmen; Léonard, Angélique ULg et al

Conference (2008, November)

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See detailCharacterization of the porous structure of biodegradable scaffolds obtained with supercritical CO2 as foaming agent
Léonard, Angélique ULg; Calberg, Cédric ULg; Kerckhofs, Greet ULg et al

in Journal of Porous Materials (2008), 15(4), 397-403

Poly(ε-caprolactone) foams were prepared, via a batch process, by using supercritical CO2 as foaming agent. Their porous structure was characterized through mercury porosimetry, helium and mercury ... [more ▼]

Poly(ε-caprolactone) foams were prepared, via a batch process, by using supercritical CO2 as foaming agent. Their porous structure was characterized through mercury porosimetry, helium and mercury pycnometry, scanning electron microscopy (SEM) and X-ray microtomography observations coupled with image analysis. The pore size distributions obtained by these two latter techniques show that the pore structure is more homogeneous when the foaming process is performed under a high CO2 saturation pressure (higher than 250 bars). [less ▲]

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See detailQuantification of in Vivo Tumor Invasion and Vascularization by Computerized Image Analysis
Blacher, Silvia ULg; Jost, M.; Melen-Lamalle, Laurence ULg et al

in Microvascular Research (2008), 75(2), 169-78

The matrix-inserted surface transplantation model is an in vivo assay used to analyse the kinetics of tumor-vessel interactions during different stages of skin carcinoma progression. This system allows ... [more ▼]

The matrix-inserted surface transplantation model is an in vivo assay used to analyse the kinetics of tumor-vessel interactions during different stages of skin carcinoma progression. This system allows the study of host-tumor interface, i.e. penetration of tumor cells into normal host tissue as well as infiltration of normal host cells into the tumor. In the present study, image analysis algorithms for processing and quantifying the extent of such migratory and tissue remodeling events are presented. The proposed method is non-parametric and its originality lies in its particularity to take into account the specific geometry of tumor-host interface. This methodology is validated by evaluating the contribution of matrix metalloproteases (MMPs) in skin carcinoma invasion and vascularization through pharmacological and genetic approaches. [less ▲]

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See detailEffect of far-infrared radiation assisted drying on microstructure of banana slices: An illustrative use of X-ray microtomography in microstructural evaluation of a food product
Léonard, Angélique ULg; Blacher, Silvia ULg; Nimmol, Chatchai et al

in Journal of Food Engineering (2008), 85(1), 154-162

X-ray microtomography coupled with image analysis represents a non-destructive technique, which allows scanning an entire sample to obtain such information as total pore volume and pore size distribution ... [more ▼]

X-ray microtomography coupled with image analysis represents a non-destructive technique, which allows scanning an entire sample to obtain such information as total pore volume and pore size distribution without the need of serial cuts as in the case of scanning electron microscopy (SEM). The technique has been applied successfully to obtain reliable microstructural information of many products undergoing different physical and chemical processes. However, the technique has still found limited use in food processing. To illustrate the use of X-ray microtomography the technique was applied to investigate the effect of far-infrared radiation (FIR) assisted drying on microstructure of a food product viz. banana. Two representative drying techniques, i.e., low-pressure superheated steam drying (LPSSD) and vacuum drying (VACUUM) were tested. Banana slices were dried by LPSSD-FIR at two different temperatures (80 and 90 degrees C) at a fixed pressure of 7 kPa. The total pore volume and pore size distribution of dried banana slices were then determined using X-ray microtomography. The results were also compared with those of products dried by LPSSD without FIR. Far-infrared radiation was found to modify the structure of the dried bananas by increasing their final porosity. The same effect of FIR was also observed in the case of vacuum drying with FIR (VACUUM-FIR). An increase of the drying temperature was also found to globally lead to an increase in the final porosity of the products. (C) 2007 Elsevier Ltd. All rights reserved. [less ▲]

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See detailFormation mechanism of Y-junctions in arrays of multi-walled carbon nanotubes
Zilli, Dario; Blacher, Silvia ULg; Cukierman, Ana Lea et al

in Colloids and Surfaces A : Physicochemical and Engineering Aspects (2008), 327(1-3), 140-143

Transmission electron microscopy of arrays of multi-walled carbon nanotubes reveals the presence of numerous Y-junctions. The diameter distributions of the samples are found to be bimodal, with one mode ... [more ▼]

Transmission electron microscopy of arrays of multi-walled carbon nanotubes reveals the presence of numerous Y-junctions. The diameter distributions of the samples are found to be bimodal, with one mode corresponding to the branches and the other mode to the trunk of the Y-junctions. The analysis of the micrographs shows that the junctions form via the merger of metal particles during the tip-growth of the tubes. (C) 2008 Elsevier B.V. All rights reserved. [less ▲]

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See detailVascular architecture of breast cancer xenographs over-expressing MT4-MMP
Chabottaux, V; Thiry, Marc ULg; Blacher, Silvia ULg et al

in Acta Clinica Belgica (2008), 63

Detailed reference viewed: 4 (1 ULg)