References of "Blacher, Silvia"
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See detailBone marrow-derived mesenchymal cells and MMP13 contribute to experimental choroidal neovascularization.
Lecomte, Julie ULg; Louis, Krystel; Detry, Benoît ULg et al

in Cellular and Molecular Life Sciences : CMLS (2011), 68

In this study, we evaluate the potential involvement of collagenase-3 (MMP13), a matrix metalloproteinase (MMP) family member, in the exudative form of age-related macular degeneration characterized by a ... [more ▼]

In this study, we evaluate the potential involvement of collagenase-3 (MMP13), a matrix metalloproteinase (MMP) family member, in the exudative form of age-related macular degeneration characterized by a neovascularisation into the choroid. RT-PCR analysis revealed that human neovascular membranes issued from patients with AMD expressed high levels of Mmp13. The contribution of MMP13 in choroidal neovascularization (CNV) formation was explored by using a murine model of laser-induced CNV and applying it to wild-type mice (WT) and Mmp13-deficient mice (Mmp13 ( -/- ) mice). Angiogenic and inflammatory reactions were explored by immunohistochemistry. The implication of bone marrow (BM)-derived cells was determined by BM engraftment into irradiated mice and by injecting mesenchymal stem cells (MSC) isolated from WT BM. The deficiency of Mmp13 impaired CNV formation which was fully restored by WT BM engraftment and partially rescued by several injections of WT MSC. The present study sheds light on a novel function of MMP13 during BM-dependent choroidal vascularization and provides evidence for a role for MSC in the pathogenesis of CNV. [less ▲]

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See detailThe Angiostatic Protein 16K Human Prolactin Significantly Prevents Tumor-Induced Lymphangiogenesis by Affecting Lymphatic Endothelial Cells.
Kinet, Virginie; Castermans, K; Herkenne, Stéphanie ULg et al

in Endocrinology (2011)

The 16-kDa angiostatic N-terminal fragment of human prolactin (16K hPRL) has been reported to be a new potent anticancer compound. This protein has already proven its efficiency in several mouse tumor ... [more ▼]

The 16-kDa angiostatic N-terminal fragment of human prolactin (16K hPRL) has been reported to be a new potent anticancer compound. This protein has already proven its efficiency in several mouse tumor models in which it prevented tumor-induced angiogenesis and delayed tumor growth. In addition to angiogenesis, tumors also stimulate the formation of lymphatic vessels, which contribute to tumor cell dissemination and metastasis. However, the role of 16K hPRL in tumor-induced lymphangiogenesis has never been investigated. We establish in vitro that 16K hPRL induces apoptosis and inhibits proliferation, migration, and tube formation of human dermal lymphatic microvascular endothelial cells. In addition, in a B16F10 melanoma mouse model, we found a decreased number of lymphatic vessels in the primary tumor and in the sentinel lymph nodes after 16K hPRL treatment. This decrease is accompanied by a significant diminished expression of lymphangiogenic markers in primary tumors and sentinel lymph nodes as determined by quantitative RT-PCR. These results suggest, for the first time, that 16K hPRL is a lymphangiostatic as well as an angiostatic agent with antitumor properties. [less ▲]

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See detailKrypton adsorption as a suitable tool for surface characterization of multi-walled CNTs
Zilli, Dario; Bonelli, P.; Gommes, Cédric ULg et al

in Carbon (2011), 49

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See detailThree-dimensional void space structure of activated carbon packed beds
Almazan Almazan, Maria Del Carmen; Léonard, Angélique ULg; Job, Nathalie ULg et al

in Journal of Porous Materials (2011), 18

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See detailInfluence of carbon xerogel textural properties on the dynamic adsorption of methyl iodide
Almazán-Almazán, M. C.; López-Domingo, F. J.; Domingo-García, M. et al

in Chemical Engineering Journal (2011), 173(1), 19-28

X-ray microtomography coupled to image analysis has been used to study the influence of the adsorbent pore texture and the experimental conditions on the dynamic adsorption of methyl iodide in packed ... [more ▼]

X-ray microtomography coupled to image analysis has been used to study the influence of the adsorbent pore texture and the experimental conditions on the dynamic adsorption of methyl iodide in packed filters. By applying this imaging technique the internal axial adsorption profiles for increasing exposure times to the gas stream are analysed. This experimental technique establishes a new technology to study in situ the dynamic adsorption of volatile compounds. Resorcinol-formaldehyde based carbon xerogels have been used as adsorbents, as their pore texture can be tuned by changing the synthesis conditions. The textural characteristics of the adsorbents (surface areas and pore volumes) have been assessed by using nitrogen and carbon dioxide adsorption as well as mercury porosimetry. The methyl iodide dynamic adsorption results show that, for the same gas flow rate and CH3I inlet concentration, the adsorbed amount is highly dependent on large pore volumes. Thus, samples with almost the same micropore volumes (adsorption volumes) have different methyl iodide adsorption capacities, which are related to, the above mentioned, large pores. The influence of both the gas carrier flow rate and the methyl iodide inlet concentration on the adsorption can be explained using the so-called linear driving force model. This approach takes into account the fact that internal transport limitations are directly related to the pore texture. Moreover, the simulation of the dynamic adsorption process has allowed relating the simulated axial concentration profiles to the experimental X-ray microtomography data. [less ▲]

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See detailA comparison of physical activation of carbon xerogels with carbon dioxide with chemical activation using hydroxides
Contreras, María S; Páez, Carlos A; Zubizarreta, Leire et al

in Carbon (2010), 48(11), 3157-3168

Carbon xerogels synthesized with a fixed resorcinol/sodium carbonate molar ratio (R/C) were physically activated using CO2. The effect of activation temperature and activation time on the final properties ... [more ▼]

Carbon xerogels synthesized with a fixed resorcinol/sodium carbonate molar ratio (R/C) were physically activated using CO2. The effect of activation temperature and activation time on the final properties of the activated carbon xerogels was evaluated. The specific surface area increases from ~600 m2 g-1 to 2000 m2 g-1 and more by increasing the temperature and duration of the activation step. A comparison between physical activation with CO2 and chemical activation with hydroxides was also performed: it was found that both processes produce an increase of the micropore volume and specific surface area without altering the meso-macroporosity developed during the synthesis. However, chemical activation can lead to the development of the narrow microporosity mainly whereas, in physical activation, the widening of the narrow micropores takes place whatever the process conditions. [less ▲]

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See detailActivated-carbon-xerogel/TiO2-P25 composite photocatalysts with high activity for the degradation of p-nitrophenol
Páez Martínez, Carlos ULg; Contreras, María; Olivera-Fuentes, Claudio et al

Poster (2010, July)

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See detail16K human prolactin is an anti-lymphangiogenic factor in vitro and in vivo
Kinet, Virginie; Castermans, Karolien; Blacher, Silvia ULg et al

Poster (2010, May 21)

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See detail16K human prolactin is an anti-lymphangiogenic factor in vitro and in vivo
Kinet, Virginie; Castermans, Karolien; Blacher, Silvia ULg et al

Poster (2010, March)

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See detailhCG: a pregnancy-related hormone stimulating angiogenesis and pericyte recruitment
Berndt, S; Blacher, Silvia ULg; Perrier d’Hauterive, S et al

Poster (2010)

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See detailNovel HDAC/DNMT twin inhibitors interfere with angiogenesis
Shiva Shankar, Thammadihalli Veerasangaiah ULg; Sulka, Béatrice; Blacher, Silvia ULg et al

Poster (2010)

DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA ... [more ▼]

DNA methylation and histone deacetylation are two key epigenetic modifications that play central role in regulation of gene expression. Several studies have shown that histone deacetylases (HDAC) and DNA methyltransferases (DNMT) inhibitors are potent antiangiogenic compounds. Though combination of HDAC and DNMT inhibitors are now being examined in clinical trials of hematological malignancies, very little work has been done to understand the effect of this combination on normal and tumoral angiogenesis. We have designed and tested a family of twin drugs with intrinsic HDAC and DNMT inhibitory activities in relevant models of angiogenesis in vitro (endothelial cells, pericytes and the 3D aortic ring assay) and in vivo (the chick chorioallantoic membrane assay). We have identified a lead compound having quantifiable antiangiogenic effect without cytotoxicity associated with increased global acetylation and decreased DNA methylation levels. This compound is presently used to develop effective approaches to treat cancer by modulating the process of angiogenesis. [less ▲]

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See detailDoes plasminogen activator inhibitor-1 drive lymphangiogenesis?
Bruyere, Francoise; Melen-Lamalle, Laurence; Blacher, Silvia ULg et al

in PLoS ONE (2010), 5(3), 9653

The purpose of this study is to explore the function of plasminogen activator inhibitor-1 (PAI-1) during pathological lymphangiogenesis. PAI-1, the main physiological inhibitor of plasminogen activators ... [more ▼]

The purpose of this study is to explore the function of plasminogen activator inhibitor-1 (PAI-1) during pathological lymphangiogenesis. PAI-1, the main physiological inhibitor of plasminogen activators is involved in pathological angiogenesis at least by controlling extracellular proteolysis and by regulating endothelial cell survival and migration. Protease system's role in lymphangiogenesis is unknown yet. Thus, based on its important pro-angiogenic effect, we hypothesized that PAI-1 may regulate lymphangiogenesis associated at least with metastatic dissemination of cancer cells. To address this issue, we studied the impact of PAI-1 deficiency in various murine models of tumoral lymphangiogenesis. Wild-type PAI-1 proficient mice were used as controls. We provide for the first time evidence that PAI-1 is dispensable for tumoral lymphangiogenesis associated with breast cancers either induced by mammary carcinoma cell injection or spontaneously appearing in transgenic mice expressing the polyomavirus middle T antigen (PymT) under the control of a mouse mammary tumor virus long-terminal repeat promoter (MMTV-LTR). We also investigated inflammation-related lymphatic vessel recruitment by using two inflammatory models. PAI-1 deficiency did neither affect the development of lymphangioma nor burn-induced corneal lymphangiogenesis. These novel data suggest that vascular remodelling associated with lymphangiogenesis and angiogenesis involve different molecular determinants. PAI-1 does not appear as a potential therapeutic target to counteract pathological lymphangiogenesis. [less ▲]

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See detailSoluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
LORQUET, Sophie ULg; Berndt, Sarah; Blacher, Silvia ULg et al

in FASEB Journal (2010), 24(10), 3782-95

Two soluble forms of vascular endothelial growth factor (VEGF) receptors, sVEGFR-1 and sVEGFR-2, are physiologically released and overproduced in some pathologies. They are known to act as anti-VEGF ... [more ▼]

Two soluble forms of vascular endothelial growth factor (VEGF) receptors, sVEGFR-1 and sVEGFR-2, are physiologically released and overproduced in some pathologies. They are known to act as anti-VEGF agents. Here, we report that these soluble receptors contribute to vessel maturation by mediating a dialogue between endothelial cells (EC) and mural cells that leads to blood vessel stabilization. Through a multidisciplinary approach, we provide evidences that these soluble VEGF receptors promote mural cell migration through a paracrine mechanism involving interplay in EC between VEGF/VEGFR-2 and sphingosine-1- phosphate type-1 (S1P)/S1P1 pathways that leads to endothelial nitric oxyde synthase (eNOS) activation. This new paradigm is supported by the finding that sVEGFR-1 and -2: 1) induce an eNOS-dependent outgrowth of a mural cell network in an ex vivo model of angiogenesis, 2) increase the mural cell coverage of neovessels in vitro and in vivo, 3) promote mural cell migration towards EC, 4) stimulate endothelial S1P1 overproduction and eNOS activation that promote the migration and the recruitment of neighboring mural cells. These findings provide new insights into mechanisms regulating physiological and pathological angiogenesis and vessel stabilization. [less ▲]

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See detailHigher sensitivity of Adamts12-deficient mice to tumor growth and angiogenesis.
El Hour, Mehdi ULg; Moncada-Pazos, A.; Blacher, Silvia ULg et al

in Oncogene (2010), 29(20), 3025-32

ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin motifs) constitute a family of endopeptidases related to matrix metalloproteinases. These proteases have been largely implicated in ... [more ▼]

ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin motifs) constitute a family of endopeptidases related to matrix metalloproteinases. These proteases have been largely implicated in tissue remodeling and angiogenesis associated with physiological and pathological processes. To elucidate the in vivo functions of ADAMTS-12, we have generated a knockout mouse strain (Adamts12−/−) in which Adamts12 gene was deleted. The mutant mice had normal gestations and no apparent defects in growth, life span and fertility. By applying three different in vivo models of angiogenesis (malignant keratinocyte transplantation, Matrigel plug and aortic ring assays) to Adamts12−/− mice, we provide evidence for a protective effect of this host enzyme toward angiogenesis and cancer progression. In the absence of Adamts-12, both the angiogenic response and tumor invasion into host tissue were increased. Complementing results were obtained by using medium conditioned by cells overexpressing human ADAMTS-12, which inhibited vessel outgrowth in the aortic ring assay. This angioinhibitory effect of ADAMTS-12 was independent of its enzymatic activity as a mutated inactive form of the enzyme was similarly efficient in inhibiting endothelial cell sprouting in the aortic ring assay than the wild-type form. Altogether, our results show that ADAMTS-12 displays antiangiogenic properties and protect the host toward tumor progression. [less ▲]

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See detailImage analysis of pearlite spheroidization based on the morphological characterization of cementite particles
Nutal, Nicolas; Gommes, Cédric ULg; Blacher, Silvia ULg et al

in Image Analysis and Stereology (2010), 29

Pearlite spheroidization is a metallurgical process in steels by which cementite lamellaer decompose into spheroids, a process accompanied by a decrease of the Vickers hardness of the samples. In thi ... [more ▼]

Pearlite spheroidization is a metallurgical process in steels by which cementite lamellaer decompose into spheroids, a process accompanied by a decrease of the Vickers hardness of the samples. In thi study, different methods are compared for measuring cementite particles length and width from scanning electron micrographs. Based on a test-image, a so-called ribbon-like method is proposed for measuring particles length and width, and for discriminating lamellae from spheroids. Differently heat-treated samples are prepared and characterized. The results of analysis are used to rationalize the evolution of the microstructure of the samples. Compared to teh calssical DeHoff shape factor, it is shown that new insight into the spheroidization process is gained by analysing the lamellar length and widh distributions. [less ▲]

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See detailHistological and transcriptional study of angiogenesis and lymphangiogenesis in uninvolved skin, acute pinpoint lesions and established psoriasis plaques: an approach of vascular development chronology in psoriasis
Henno, Audrey ULg; Blacher, Silvia ULg; Lambert, Charles ULg et al

in Journal of Dermatological Science (2010), 57(3), 162-169

Background Dysregulation of angiogenesis and lymphangiogenesis could participate in psoriasis pathogenesis. Analysis of nascent psoriasis lesions should help at identifying early vascular anomalies ... [more ▼]

Background Dysregulation of angiogenesis and lymphangiogenesis could participate in psoriasis pathogenesis. Analysis of nascent psoriasis lesions should help at identifying early vascular anomalies. Objective To analyse vascular development, angiogenesis and lymphangiogenesis markers expression in uninvolved skin in psoriatic patients (N), early psoriasis lesions or pinpoints (PP) and psoriasis plaques (PSO). Methods Skin biopsies were taken in 17 patients in N and in PSO and/or PP. The mRNA steady-state level of angiogenesis and lymphangiogenesis markers was measured by RT-PCR. Immunohistochemistry was performed for von Willebrand factor, podoplanin, Ki-67 and VEGFR3. Blood (BV) and lymphatic (LV) vessels expansion was measured by computer-assisted morphometry. Results Clinical and epidermal aspects indicated that PP are intermediate between N and PSO. While total BV area was already increased in PP similarly to PSO as compared to N, LV area in PP was intermediate between N and PSO. Mean LV size was identical in N and PP and increased in PSO, mean BV size in PP being intermediate between N and PSO. VEGF-A 189 variant was increased in PP as compared to N and PSO. As compared to N, angiogenesis markers (VEGF-A isoforms, PlGF, VEGFR2, NRP-1), VEGF-C and NRP-2 were similarly increased in PP and PSO. Keratin 16 and the lymphangiogenesis markers (VEGFR3, prox-1) were intermediate in PP. Conclusion These data suggest that the expansion of lymphatic vessels occurs after blood vascular development in psoriasis. Expansion of BV in PP could be followed by vessel enlargement during progression to PSO, in parallel with a decreased VEGF-A 189/VEGF-A 121 balance in plaques [less ▲]

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See detailNew Biological Investigations on 3-Bromophenyl 6-Acetoxymethyl-2-oxo-2H-1-Benzopyran-3-Carboxylate as Anti-angiogenic Agent
Hemmer, M.; Kempen, Isabelle; De Tullio, Pascal ULg et al

in Drug Development Research (2010), 71

The development of blood vessels inside tumors is required to provide the nutrients and oxygen needed for tumor growth and to allow the spread of cancer cells at a distance to form metastasis ... [more ▼]

The development of blood vessels inside tumors is required to provide the nutrients and oxygen needed for tumor growth and to allow the spread of cancer cells at a distance to form metastasis. Angiogenesis is also implicated in ocular diseases like age-related macular degeneration. The present work describes the potential anti-angiogenic properties of a coumarinic derivative, 3-bromophenyl 6-acetoxymethyl-2-oxo-2H-1-benzopyran-3-carboxylate (IK9), previously described as a potent inhibitor of HT 1080 fibrosarcoma cell invasion in vitro and tumor growth in vivo. In vivo, ex vivo, and in vitro models were used to delineate the anti-angiogenic properties of IK9. The anti-angiogenic effect of IK9 was demonstrated in vivo in a choroidal neovascularization mice model and additionally ex vivo in a rat aortic ring assay where it was more active than the known matrix metalloproteinase inhibitor Ro 28-2653. IK9 did not affect apoptosis, proliferation, or endothelial cell invasiveness in vitro. These findings suggest a complex mechanism of action of the compound via direct or indirect effects on endothelial cell properties. This study identifies IK9 as a new potent inhibitor of angiogenesis and suggests its potential use as a therapeutic agent. [less ▲]

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See detailPractical Methods for Measuring the Tortuosity of Porous Materials from Binary or Gray-Tone Tomographic Reconstructions
Gommes, Cédric ULg; Bons, Anton-Jan; Blacher, Silvia ULg et al

in AIChE Journal (2009), 55(8), 2000-2012

Two practical methods are proposed to measure the tortuosity of a porous or permeable material from its tomographic reconstruction. The first method is based on the direct measurement of the shortest ... [more ▼]

Two practical methods are proposed to measure the tortuosity of a porous or permeable material from its tomographic reconstruction. The first method is based on the direct measurement of the shortest distance between two points in the pores, and the second is based on the geodesic reconstruction of the pore or permeation space. Unlike the first method, the second can be directly applied to gray-tone tomograms, without the need of a segmentation step. The methods are illustrated with an electron tomogram of clay/plastic nanocomposite, an X-ray microtomogram of sandstone, and a series of model morphologies consisting of penetrable random spheres. For the latter series, the measured tortuosities compare very well with those derived independently from the theoretical effective diffusion coefficients [less ▲]

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See detailEvaluation of the dynamic adsorption of gas on active carbon beds using in situ X-ray µ-tomography coupled with 3D image analysis
Almazan, Maria del Carmen; Léonard, Angélique ULg; Lodewyckx, Peter et al

Poster (2009, June)

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