References of "Bettendorff, Lucien"
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See detailSolubilization of Sodium Channel from Human Brain
De Rycker, C.; Grandfils, Christian ULg; Bettendorff, Lucien ULg et al

in Journal of Neurochemistry (1989), 52(2), 349-53

[3H]Tetrodotoxin binds to a single class of receptor sites in homogenates of human brain with a KD of 9.1 nM at 0 degree C and a maximal binding capacity of 5.9 pmol/mg of protein. This tetrodotoxin ... [more ▼]

[3H]Tetrodotoxin binds to a single class of receptor sites in homogenates of human brain with a KD of 9.1 nM at 0 degree C and a maximal binding capacity of 5.9 pmol/mg of protein. This tetrodotoxin receptor has been solubilized, and several parameters influencing the efficiency of this critical step have been studied. Treatment of brain membranes with 2% (wt/vol) Nonidet P-40 solubilizes up to 38% of the tetrodotoxin receptor sites. The duration of this solubilization step must not exceed 15 min at an optimal pH of 6.8. The binding activity is most stable when exogenous phosphatidylcholine is added to the soluble receptor with a phosphatidylcholine/detergent ratio of 1:5. [less ▲]

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See detailPhosphorylated Thiamine Derivatives and Cortical Activity in the Baboon Papio Papio: Effect of Intermittent Light Stimulation
Bettendorff, Lucien ULg; Schoffeniels, Ernest; Naquet, R. et al

in Journal of Neurochemistry (1989), 53(1), 80-87

The effect of intermittent light stimulation (ILS) on the distribution of thiamine derivatives in three brain areas (occipital, motor, and premotor) was compared in photosensitive and nonphotosensitive ... [more ▼]

The effect of intermittent light stimulation (ILS) on the distribution of thiamine derivatives in three brain areas (occipital, motor, and premotor) was compared in photosensitive and nonphotosensitive baboons. ILS induces paroxysmal discharges in the motor and premotor areas of photosensitive animals only. In baboons submitted to ILS, thiamine triphosphate (TTP) decreases in both photosensitive and nonphotosensitive animals; thiamine monophosphate (TMP) increases in photosensitive animals, which present ILS-induced paroxysmal discharges, whereas it is unaffected in nonphotosensitive animals. The variations are the most significant in the occipital (visual) cortex. A consumption of TTP may result from electrical activity induced by light stimulation in the occipital area. No correlation between ILS-induced paroxysmal activity and a decrease in TTP contents was found. However, photosensitive animals are affected differently from nonphotosensitive animals, as their content of TMP in the cerebral cortex increases on stimulation. However, as long as the exact role of thiamine compounds in relation to membrane excitability in the nervous system remains unknown, it is impossible to conclude whether the differences observed in the metabolism of thiamine compounds are the cause or the consequence of the photosensitivity in the baboon Papio papio. [less ▲]

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See detailTriphosphate de thiamine et bioélectrogenèse
Bettendorff, Lucien ULg

Doctoral thesis (1988)

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See detailSynthesis of [Gamma-32p]Thiamine Triphosphate
Grandfils, Christian ULg; Bettendorff, Lucien ULg; de Rycker, C. et al

in Analytical Biochemistry (1988), 169(2), 274-278

We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and ... [more ▼]

We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange purification steps, the thiamine derivative has a specific radioactivity of 10 Ci/mmol. The average final yield synthesis is about 10%. [less ▲]

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See detailThiamine Triphosphatase from Electrophorus Electric Organ Is Anion-Dependent and Irreversibly Inhibited by 4,4'-Diisothiocyanostilbene-2,2'disulfonic Acid
Bettendorff, Lucien ULg; Wins, Pierre; Schoffeniels, Ernest

in Biochemical and Biophysical Research Communications (1988), 154(3), 942-947

Thiamine triphosphatase (TTPase) from membranes isolated from the main electric organ of E. electricus is activated about 8 fold by NO3-, I- and SCN- while SO42- is inhibitory. Activating anions shift the ... [more ▼]

Thiamine triphosphatase (TTPase) from membranes isolated from the main electric organ of E. electricus is activated about 8 fold by NO3-, I- and SCN- while SO42- is inhibitory. Activating anions shift the pH optimum of the enzyme from 5.0 to 8.0. The enzyme is irreversibly inactivated by low concentrations of 4,4'-diisothiocyano-2,2' disulfonic acid (DIDS), an inhibitor of anion transport. Anions protect from DIDS inactivation. These and other results suggest that the membrane-bound TTPase activity is tightly controlled, possibly through mechanisms involving anion transport. [less ▲]

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See detailActivités thiamine triphophatasiques et bioélectrogenèse
Bettendorff, Lucien ULg; Schoffeniels, Ernest

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1987), 142(2-3), 228-236

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See detailThiamine triphosphate and membrane-associated thiamine phosphatases in the electric organ of Electrophorus electricus
Bettendorff, Lucien ULg; Michel-Cahay, Colette; Grandfils, Christian ULg et al

in Journal of Neurochemistry (1987), 49

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See detailDetermination of thiamine and its phosphate esters in human blood serum at femtomole levels
Bettendorff, Lucien ULg; Grandfils, Christian ULg; De Rycker, Cécile et al

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (1986), 382

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See detailMembrane associated thiamine phosphatases in the electric organ of Electrophorus electricus
Michel-Cahay, C; Bettendorff, Lucien ULg; De Rycker, C et al

Poster (1985, September 03)

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See detailDérivés phosphorylés de la thiamine et bioélectrogenèse
Grandfils, Christian ULg; Bettendorff, Lucien ULg; Schoffeniels, E et al

in Regards sur la biochimie (1985), 4

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See detailMembrane associated thiamin phosphatases in the electric organ of E. electricus
Michel-Cahay, C; Bettendorff, Lucien ULg; De Rycker, C et al

in Archives Internationales de Physiologie et de Biochimie (1985), 93

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See detailHigh performance gel filtration as a tool for the monitoring of the purification steps of the Na channel protein
Bontemps, J; Grandfils, Christian ULg; Bettendorff, Lucien ULg et al

in Archives Internationales de Physiologie et de Biochimie (1985), 93

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See detailA purification procedure of the tetrodotoxin binding component contained in the electroplaxes of E. electricus
Dandrifosse, Guy ULg; Grandfils, Christian ULg; Bettendorff, Lucien ULg et al

in Changeux, J.P.; Maelicke, A.; Neumann, E. (Eds.) Molecular Basis of Nerve Activity (1985)

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See detailRapid purification procedure of the Na-channel of Electrophorus electricus
Dandrifosse, Guy; Grandfils, Christian ULg; Bettendorff, Lucien ULg et al

in Changeux, Jean-Pierre; Maelicke, A.; Neumann, E. (Eds.) Molecular Basis of Nerve Activity (1985)

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See detailDetermination of thiamine and its phosphate esters by reversed-phase HPLC at femtomole levels. Application to bioelectrogenesis.
Bettendorff, Lucien ULg; Schoffeniels, Ernest; Schmartz, Denis et al

in Changeux, Jean-Pierre; Maelicke, A.; Neumann, E. (Eds.) Molecular Basis of Nerve Activity (1985)

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See detailHigh yield synthesis of a new Na-channel marker, [3H]-ethylenediamine ditetrodotoxin, EDD-TTX.
Grandfils, Christian ULg; Bettendorff, Lucien ULg; Schoffeniels, Ernest et al

in Changeux, Jean-Pierre; Maelicke, A.; Neumann, E. (Eds.) Molecular Basis of Nerve Activity (1985)

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See detailDérivés phosphorylés de la thiamine et bioélectrogenèse
Grandfils, Christian ULg; Bettendorff, Lucien ULg; Schoffeniels, E et al

Poster (1984, September)

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See detailHPLC in neurochemistry
Bontemps, J; Laschet, L; Bettendorff, Lucien ULg et al

Poster (1984, May 18)

Detailed reference viewed: 11 (0 ULg)