References of "Beckers, Yves"
     in
Bookmark and Share    
Full Text
See detailUtilisation des enzymes en alimentation porcine et avicole
Beckers, Yves ULg; Piron, Fabien ULg

in CRA-W (Ed.) Impact de l'alimentation sur la santé animale : nouveaux développements (2009)

Detailed reference viewed: 57 (15 ULg)
Full Text
Peer Reviewed
See detailHen egg yolk antibodies (IgY), production and use for passive immunization against bacterial enteric infections in chicken : a review
Chalghoumi, Raja; Beckers, Yves ULg; Portetelle, Daniel ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2009), 13(3), 295-308

Enteric infections caused by Salmonella remain a major public health burden worldwide. Poultry, particularly chickens, are known to be the main reservoir for this zoonotic pathogen. Therefore, the ... [more ▼]

Enteric infections caused by Salmonella remain a major public health burden worldwide. Poultry, particularly chickens, are known to be the main reservoir for this zoonotic pathogen. Therefore, the prevention and monitoring of Salmonella infection during the live phase may greatly reduce the contamination of poultry meat during slaughter and processing. With the ban on sub-therapeutic antibiotic usage in Europe and the increasingly strictness of the European legislation on food hygiene, passive immunization by oral administration of pathogen-specific hen egg yolk antibody (IgY) may be a useful and attractive alternative. This review offers summarized information about IgY production and the use of these antibodies for passive immunization, particularly in poultry [less ▲]

Detailed reference viewed: 195 (5 ULg)
Full Text
Peer Reviewed
See detailLes coproduits européens issus du bioéthanol produit au départ de céréales ont une composition chimique variable
Piron, Fabien ULg; Bruyer, Denis; Thewis, André ULg et al

in journées de la recherche avicole (2009)

There is worldwide increasing interest in producing ethanol from grains. Currently, maize is the main grain used in fuel ethanol production (particularly in the USA). However, in Western Europe and Canada ... [more ▼]

There is worldwide increasing interest in producing ethanol from grains. Currently, maize is the main grain used in fuel ethanol production (particularly in the USA). However, in Western Europe and Canada, wheat is the main grain for ethanol plants. Barley, rye, sorghum or combinations of grains are also used. The process of ethanol production from grains generates by-products (distillers’ grains, solubles and/or gluten feed) with potential in livestock feed. Consequently, a better knowledge of chemical composition of these by-products is essential. The aim of the present study was to chemically characterise 14 batches of by-products (mainly from wheat, but also from barley and maize) purchased from some plants in Western Europe. Starch was almost completely removed by ethanol production process. Consequently, crude protein and fibre were concentrated in by-products with regard to grain value. Ratios of by-products to grain (wheat is reference) varied between 1.6 and 3.2 (protein), 1.6 and 2.7 (NDF) and 2.3 and 5.1(ADF). Furthermore, heat treatment used during the production process of dried distillers’ grains could cause destruction of important amounts of some amino acids, especially lysine (Maillard reactions). Ratios of lysine to crude protein varied among batches of byproducts and were particularly poor and unstable for distillers’ grains. In conclusion, chemical composition of European by-products of ethanol fuel produced from grains is variable. It is particularly important for wheat-based distillers’ grains. More information is needed about digestibility and availability of nutriments from these by-products. Particularly, lysine concentration and availability are probably one of the main concerns for nutritive value of by-products of ethanol fuel produced from grains. [less ▲]

Detailed reference viewed: 39 (4 ULg)
Full Text
Peer Reviewed
See detailWheat-based dried distillers’ grains are variable in chemical composition
Piron, Fabien ULg; Bruyer, Denis; Thewis, André ULg et al

in Garnsworthy, P., C.; Wiseman, J. (Eds.) Recent Advances in Animal Nutrition 2008 (2009)

Detailed reference viewed: 22 (5 ULg)
Full Text
See detailThe role of the specific immune response on cecal colonization of slow-growing broiler chickens reared on litter contaminated by Campylobacter jejuni
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Beckers, Yves ULg et al

Conference (2009)

The aim of this experiment was to study the relation between the specific immune response and infection of slow-growing broiler chickens reared on straw litter contaminated by Campylobacter jejuni. The 11 ... [more ▼]

The aim of this experiment was to study the relation between the specific immune response and infection of slow-growing broiler chickens reared on straw litter contaminated by Campylobacter jejuni. The 11-weeks experiment was carried out in a 45 m2 broiler house with 500 chickens from 1 day of age, which had access to an open-air range. Litter contamination was performed before the setting up of the chicks by rearing campylobacter-positive cocks. These birds were also used to contaminate the open-air range during one week before the exit of the chickens, at 5 weeks of age. Blood samples were taken weekly on 20 chickens for anti-campylobacter IgG quantitative measurements on serum. Chickens were then euthanized and cecal contents and bile were collected for Campylobacter enumeration and anti-campylobacter IgA quantification, respectively. A high serum anti-campylobacter IgG titer of 110.13 was measured in one-day-old chicks. The IgG antibodies level significantly decreased to 5.60 at 28 days of age which coincided with the onset of Campylobacter infection in the flock. A significant reduction of the cecal colonization from 11 weeks of age was correlated with the increase in biliary IgA titers. These results suggest that the 2-3 week’s delay generally observed in poultry production before flock infection by Campylobacter may be, at least partly, explained by the protective effect of maternal antibodies. The increase of secretory immune response as bird age may help to limit Campylobacter population in the chicken gut. [less ▲]

Detailed reference viewed: 70 (23 ULg)
Full Text
Peer Reviewed
See detailAdhesion and growth inhibitory effect of chicken egg yolk antibody (IgY) on Salmonella enterica serovars Enteritidis and Typhimurium in vitro.
Chalghoumi, Raja; Thewis, André ULg; Beckers, Yves ULg et al

in Foodborne Pathogens and Disease (2009), 6(5), 593-604

The protective effects of powder preparation of egg yolk immunoglobulin Y (IgY), specific to Salmonella Enteritidis and Salmonella Typhimurium outer membrane proteins (OMP), against these two Salmonella ... [more ▼]

The protective effects of powder preparation of egg yolk immunoglobulin Y (IgY), specific to Salmonella Enteritidis and Salmonella Typhimurium outer membrane proteins (OMP), against these two Salmonella sp. serovars were investigated in vitro in two different assays: adhesion-prevention and growth-inhibition. The adhesion-prevention assay was conducted using polarized monolayers of the human intestinal epithelial Caco-2 cell line. First, the conditions of Salmonella adherence to Caco-2 cells were optimized, and interferences of bacteria with the transepithelial electrical resistance (TER) of fully differentiated Caco-2 cell monolayers and the lactate dehydrogenase release upon exposure of the cells to Salmonella were evaluated. Both Salmonella sp. serovars were able to adhere to Caco-2 cells and decreased TER. Results from the adhesion-prevention assay demonstrated that specific IgY reduced the decrease in TER of the infected Caco-2 cell monolayers and blocked the Salmonella sp. adhesion in a concentration-dependent manner (p < 0.05). Nonspecific IgY also exhibited an inhibitory effect on these two parameters, but to a lesser extent than that of the specific IgY (p < 0.05). The protective effect of nonspecific IgY could be attributed to the low-density lipoprotein component of the water-soluble fraction of egg yolks that may not have been eliminated during ultrafiltration. The growth-inhibition assay revealed that specific IgY had an inhibitory effect on the bacterial growth, markedly during the late exponential phase, whereas nonspecific IgY failed to do so. Taken together, these results suggest that the in vitro growth inhibitory effect of specific IgY on Salmonella spp. resulted from the specific binding activity of these IgY to Salmonella sp. OMP. Passive immunization with Salmonella sp. OMP-specific IgY could thus be useful to prevent Salmonella colonization in broiler chickens and the subsequent carcass contamination during processing. [less ▲]

Detailed reference viewed: 107 (32 ULg)
Full Text
Peer Reviewed
See detailEffects of feed supplementation with specific hen egg yolk antibody (immunoglobin Y) on Salmonella species cecal colonization and growth performances of challenged broiler chickens
Chalghoumi, Raja; Marcq, Christopher ULg; Thewis, André ULg et al

in Poultry Science (2009), 88(10), 2081-2092

Anti-Salmonella spp. egg yolk antibodies (IgY) simultaneously directed against Salmonella Enteritidis and Salmonella Typhimurium were tested to determine if their inclusion in feed decreased Salmonella ... [more ▼]

Anti-Salmonella spp. egg yolk antibodies (IgY) simultaneously directed against Salmonella Enteritidis and Salmonella Typhimurium were tested to determine if their inclusion in feed decreased Salmonella spp. cecal colonization in experimentally infected broiler chickens. Egg yolk powder (EYP) was obtained by freeze-drying egg yolks containing anti-Salmonella spp. Immunoglobin Y was included in feed at 5 levels of concentration (0 to 5%). Feeds were formulated to similar nutrient levels and provided for ad libitum intake from d 1 to 28. Three days after initiation of feed treatments (d 4), chickens were co-challenged with equal numbers of Salmonella Enteritidis and Salmonella Typhimurium (2x10(6) cfu/bird). Cecal samples were recovered weekly over the experimental period (d 7 to 28) to enumerate Salmonella spp. The effect of anti-Salmonella spp. IgY feed supplementation on growth performance of infected chickens was also evaluated during the same period. In comparison with the positive control treatment (PC), treatments involving EYP (T1, T2, T3, T4, and T5), whether containing anti-Salmonella spp. IgY or not, significantly improved (P<0.05) the growth performance of challenged chickens, but without reaching the performance levels of nonchallenged chickens (NC1 and NC2). However, no link can be established between the enhancement in growth performance of challenged birds and their contamination levels by Salmonella because in-feed incorporation of EYP had no significant effect on cecal colonization by Salmonella. Furthermore, the comparison of the 5 anti-Salmonella spp. IgY concentration levels in feed did not reveal any anti-Salmonella spp. IgY concentration effect on growth performance and Salmonella cecal colonization. These results suggest that anti-Salmonella spp. IgY would undergo denaturation and degradation after their passage through the animal gastrointestinal tract and reveal that components of EYP other than specific antibodies have a beneficial effect on growth performance. [less ▲]

Detailed reference viewed: 108 (38 ULg)
Full Text
Peer Reviewed
See detailDéveloppement d’une stratégie d’immunisation passive du poulet de chair vis-à-vis de Salmonella Enteritidis et Typhimurium à l’aide d’anticorps du jaune d’œuf
Marcq, Christopher ULg; Chalghoumi, RAJA ULg; Beckers, Yves ULg et al

in 8èmes JRA : Synthèses et textes (2009)

The present study investigates the potential of oral immunotherapy using hen egg yolk immunoglobulins (IgY) as a strategy to reduce the impact of Salmonella contamination on broilers. In a first step ... [more ▼]

The present study investigates the potential of oral immunotherapy using hen egg yolk immunoglobulins (IgY) as a strategy to reduce the impact of Salmonella contamination on broilers. In a first step, hyperimmune eggs were obtained from laying hens immunized using Salmonella Enteritidis (SE) and Typhimurium (ST) outer membrane proteins in emulsion with Freund adjuvants. This led to the production of high levels of IgY antibodies directed against the two Salmonella serovars in egg yolks. Furthermore, no adverse affects were detected on mortality or laying rate in hens injected with this vaccine. Powders were obtained from these eggs by spray- or freeze-drying the whole yolk. In order to study the effect of these powders on broiler’s growth performances, a challenge trial was conducted. 384 Salmonella spp.-free day-of-hatch chickens received supplemented feed (5 % freeze-dried egg yolk powder) since arrival, and were infected at day four with 106 cfu of SE and ST per animal. We found a significant improvement in growth performances for the groups receiving the yolk-enriched diets. Nevertheless, none of the supplement concentrations allowed to raise the same body weight after 28 days of complementation than in uninfected broilers (p < 0,05). Interestingly, nonimmune egg yolk powder also exhibited a positive effect on performances of broilers experimentally infected with SE and ST. Our results suggest that passive immunization through egg yolk powders could be useful to reduce negative effects of Salmonella infection on broilers growth performances. [less ▲]

Detailed reference viewed: 153 (31 ULg)
Peer Reviewed
See detailDifferent concepts of animal welfare and stakeholders arguments
Bartiaux-Thill, Nicole; Stassart, Pierre M ULg; Lamine, Claire et al

Poster (2008, September 11)

Detailed reference viewed: 14 (6 ULg)
See detailLa réduction des frais d'alimentation des vaches laitières. Quelles possibilités à court et long termes ?
Beckers, Yves ULg

Conference given outside the academic context (2008)

Detailed reference viewed: 40 (14 ULg)
Full Text
Peer Reviewed
See detailInfluence Of The Grinding Level And Extrusion On The Nutritional Value Of Lupin Seed (Lupinus Albus) For Cattle In The Context Of The Dutch Protein Evaluation System
Froidmont, E.; Bonnet, M.; Oger, Robert ULg et al

in Animal Feed Science and Technology (2008), 142(1-2),

Detailed reference viewed: 70 (50 ULg)
Full Text
Peer Reviewed
See detailContamination of poultry flocks by the human pathogen Campylobacter spp. and strategies to reduce its prevalence at the farm level.
Vandeplas, Sabrina ULg; Marcq, Christopher ULg; Dubois Dauphin, Robin et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2008), 12(3), 317-334

Enteric Campylobacter spp. bacteria are human pathogens that frequently contaminate poultry flocks. Consumption of products from poultry origin may then lead to acute bacterial enteritis called ... [more ▼]

Enteric Campylobacter spp. bacteria are human pathogens that frequently contaminate poultry flocks. Consumption of products from poultry origin may then lead to acute bacterial enteritis called campylobacteriosis of which prevalence is increasing for about ten years in Europe. This review summarizes Campylobacter epidemiological data, risk factors for contamination in poultry flocks and conceivable strategies to control this pathogen. [less ▲]

Detailed reference viewed: 266 (15 ULg)
Full Text
See detailSurvival of yolk’s immunoglobulins directed against Salmonella Enteritidis and Salmonella Typhimurium in the gastro-intestinal tract of the broiler chicken
Marcq, Christopher ULg; Graide, Virginie; Beckers, Yves ULg et al

in Proceedings of the Thirteenth Conference on Food Microbiology (2008)

Detailed reference viewed: 39 (9 ULg)
Full Text
Peer Reviewed
See detailEffect Of Rumen-Degradable Protein Balance Deficit On Voluntary Intake, Microbial Protein Synthesis, And Nitrogen Metabolism In Growing Double-Muscled Belgian Blue Bulls Fed Corn Silage-Based Diet
Valkeners, Damien; Thewis, André ULg; Van Laere, Marc et al

in Journal of Animal Science (2008), 86(3), 680-690

Two trials were conducted to evaluate the effect of rumen-degradable protein balance (OEB) deficit on voluntary intake (trial 1), microbial protein synthesis, andNmetabolism (trial 2) in growing ... [more ▼]

Two trials were conducted to evaluate the effect of rumen-degradable protein balance (OEB) deficit on voluntary intake (trial 1), microbial protein synthesis, andNmetabolism (trial 2) in growing doublemuscled Belgian Blue bulls. In trial 1, six bulls (339 ± 26 kg of initial BW) were used in a replicated 3 × 3 Latin square and received a diet of 60% corn silage and 40% concentrate with ad libitum intake (DM basis). Three concentrates were formulated by adding urea at the expense of barley to give similar dietary contents of intestinal digestible proteins, NE for fattening, and fermentable OM, but with different levels of OEB. Thus, 2 levels of OEB deficit (−23.7 and −9.2 g of OEB/kg of DM) were compared with a diet providing a slight OEB surplus (5.3 g of OEB/kg of DM). Voluntary DMI decreased linearly (P = 0.02) with decreasing rumen-degradable protein balance. This decrease in intake could explain the linear decrease in ADG observed when negative OEB diets were fed. In trial 2, six bulls (304 ± 12 kg of initial BW) with cannulas in the rumen and proximal duodenum were used in a replicated 3 × 3 Latin square and fed diets similar to those used in trial 1 at an intake level of 85 g of DM/kg of BW0.75. Diurnal variations of ruminal NH3-N and plasma urea-N concentrations were greatly influenced by the level of OEB in the diet. No differences in NDF and starch degradation in the rumen, microbial N flow at the duodenum, or efficiency of microbial protein synthesis in the rumen were noted among the levels of OEB in diets. The reductions of the OEB value from 5.3 g/kg of DM to −9.2 g/ kg of DM and −23.7 g/kg of DM were associated with reductions of 26.5 and 48.8% in urinary Noutput. Absolute amounts of N retained by the bulls increased significantly with the level of OEB in diets. Indeed, 51.4% of the incremental supply of N was excreted between −23.7 and −9.2 g of OEB/kg of DM diets, and 74.6% of the incremental supply of N was excreted between −9.2 and 5.3 g of OEB/kg of DM diets. Feeding diets characterized by an adequate intestinal digestible protein supply and a OEB close to −10 g of OEB/kg of DM could be a feeding strategy to reduce N losses from the farm with little effect on the animal performance and voluntary intake. Reduced OEB may reduce N excretion in the environment but may also result in decreased N retention. [less ▲]

Detailed reference viewed: 93 (15 ULg)
Full Text
Peer Reviewed
See detailProduction Of Hen Egg Yolk Immunoglobulins Simultaneously Directed Against Salmonella Enteritidis And Salmonella Typhimurium In The Same Egg Yolk
Chalghoumi, Raja ULg; Thewis, André ULg; Portetelle, Daniel ULg et al

in Poultry Science (2008), 87(1), 32-40

The present study was an attempt to raise hen egg yolk Ig (IgY) simultaneously directed against Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) in the same egg yolk. The immunopotentiating ... [more ▼]

The present study was an attempt to raise hen egg yolk Ig (IgY) simultaneously directed against Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) in the same egg yolk. The immunopotentiating effect of 2 different adjuvants—Freund’s adjuvants (FA) and immunostimulating complexes matrix (IM)—on antibody response was also evaluated. Bacterial outer membrane proteins (OMP) were selected as target antigens. The ISA Brown hens, specific-Salmonella spp.-free status, divided into 6 groups were intramuscularly injected with a monocompound antigen preparation: SE-OMP (treatment SEFA or SE-IM) or ST-OMP (treatment ST-FA or ST-IM), or a combined antigen preparation: ¹⁄₂ SE-OMP and ¹⁄₂ STOMP (treatment SEST-FA or SEST-IM). Titers of antibodies in yolk were evaluated biweekly with ELISA. There was no antigen × adjuvant interaction on antibody titers. Anti-SE IgY titers in hens that received treatment SESTFA or SEST-IM were statistically similar (P > 0.05) as compared with those obtained from hens immunized with treatment SE-FA or SE-IM. Anti-ST IgY titers in hens immunized with treatment SEST-FA or SEST-IM were slightly lower than those of hens that received treatment ST-FA or ST-IM. The cross-reactivity of anti-SE IgY, induced by treatment SE-FA or SE-IM, with ST-OMP antigen and that of anti-ST IgY, induced by ST-FA or ST-IM, with SE-OMP antigen were arbitrarily assessed on d 43 and 155 by ELISA. The average cross-reactivity of anti-SE IgY with ST-OMP antigen was 71.7%. The average cross-reactivity of anti-ST IgY with SE-OMP antigen was 78.8%. In FA groups, antibody titers were found higher (P < 0.05) than those in IM groups. Furthermore, no extensive lesions or clinical abnormalities were detected in hens injected with FA. These findings showed the opportunity to raise IgY antibody against 2 Salmonella serovars in the same yolk and that FA was more efficient than IM in mediating antibody response. [less ▲]

Detailed reference viewed: 89 (33 ULg)
See detailPeut-on se passer des céréales dans l'alimentation des bovins
Beckers, Yves ULg

Conference given outside the academic context (2007)

Detailed reference viewed: 32 (12 ULg)