References of "Beckers, Jean-François"
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See detailPregnancy-Associated Glycoprotein D-56 kDa [Fragment] from Dama dama placenta - Accession number C0HJD0
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein C-39 kDa [Fragment] from Dama dama placenta - Accession number C0HJC9
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein B-56 kDa [Fragment] from Dama dama placenta - Accession number C0HJC8
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein A-62 kDa [Fragment] from Dama dama placenta - Accession number C0HJC7
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-associated glycoprotein (PAG) pattern and pregnancy detection in Boer goats using an ELISA with different antisera
Shahin, M; Friedrich, M; Gauly, M et al

in Small Ruminant Research (2013), 113

Pregnancy-associated glycoproteins (PAGs) are macromolecules produced by placental tissue and released into the maternal circulation where they allow pregnancy diagnosis and follow-up. The present study ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) are macromolecules produced by placental tissue and released into the maternal circulation where they allow pregnancy diagnosis and follow-up. The present study addresses the question to what extent plasma PAG determination may serve as a means of early pregnancy detection in goats in a similar way it is practiced in cows, and whether an ovine or bovine PAG-ELISA may be utilized to this end. Blood samples were collected from eight pregnant pluriparous Boer goat does twice weekly during the first seven seeks and the last four weeks of pregnancy and weekly in-between and during four weeks following parturition. Plasma PAG concentrations (mean±SEM) were determined using a competitive enzyme-linked immunosorbent assay. Assays were conducted with polyclonal antisera raised in rabbits against purified preparations of caprine (AS#706), ovine (AS#780) and bovine PAG (AS#726). In the assay systems purified bovine PAG served as standard and tracer and goat anti-rabbit IgG served as coating antibody. With the antibody raised against caprine PAG (AS#706) a steep increase to a climax of 69±9 ng/ml on day 56 of pregnancy was followed by a gradual decline to 16±3 ng/ml at parturition and 0.3±0.07 ng/ml four weeks postpartum. The results achieved with the antiovine PAG (AS#780) showed close similarity, a maximum of 92±14 ng/ml being reached at 56 days of pregnancy. With anti-bovine PAG (AS#726), the PAG level increased to a maximum of 3.1±0.2 ng/ml on day 105 of pregnancy and fluctuated around 3 ng/ml until the end of pregnancy. The difference between pregnant and non-pregnant does reached a significant level 21 days after conception, one week earlier than with caprine and ovine antisera. [less ▲]

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See detailRider and horse salivary cortisol levels during competition and impact on performance.
Peeters, Marie ULg; Closson, Coline; Beckers, Jean-François ULg et al

in Journal of Equine Veterinary Science (2013), 33(3), 155-160

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See detailPlasma concentrations of pregnancy-associated glycoproteins measured using anti-bovine PAG-2 antibodies on Day 120 of gestation predict abortion in dairy cows naturally infected with Neospora caninum.
Garcia-ispierto, I; Almeria, S; Serrano, B et al

in Reproduction in Domestic Animals (2013), 48

The present study sought to determine: (i) the effects of Neospora caninum infection and twin pregnancy on plasma pregnancy-associated glycoprotein-2 (PAG-2) concentrations throughout pregnancy and (ii ... [more ▼]

The present study sought to determine: (i) the effects of Neospora caninum infection and twin pregnancy on plasma pregnancy-associated glycoprotein-2 (PAG-2) concentrations throughout pregnancy and (ii) whether plasma PAG-2 concentrations could predict abortion in N. caninum-infected cows. The study was performed on a commercial Holstein-Friesian dairy herd in northeastern Spain and the final data included those recorded in 53 non-aborting and 19 aborting animals. Blood samples were collected immediately before pregnancy diagnosis (on Days 40, 90, 120, 150, 180 and 210 post-insemination) in non-aborting cows or until the time of abortion detection in aborting cows. General lineal models (GLM) repeated measures ANOVA revealed the different behavior of PAG-1 and PAG-2, and significant effects of Neospora seropositivity, cool season and twin pregnancy on plasma PAG-2 concentrations throughout gestation (between-subject effects). In addition, based on the odds ratios, the likelihood of abortion increased in Neospora-seropositive cows (by a factor of 7.0) compared to seronegative animals and decreased in cows with a high plasma PAG-2 concentration (>4.5 ng/ml) on Day 120 of pregnancy (by a factor of 0.24), compared to the remaining cows. In conclusion, there is a relationship between plasma PAG-2 concentrations and the risk of abortion in Neosporainfected dairy cows. Thus, plasma PAG concentrations measured using anti-boPAG-2 antiserum on Day 120 of gestation could serve as an indicator of the abortion risk in N. caninum infected animals; values <4.5 ng/ml indicating a high risk of abortion in chronically infected animals. [less ▲]

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See detailPurification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females.
Barbato, Olimpia; Melo de Sousa, Noelita ULg; Barile, Vittoria Lucia et al

in BMC Veterinary Research (2013), 9

BACKGROUND: Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of ... [more ▼]

BACKGROUND: Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species. RESULTS: Amino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n = 33) and non-pregnant (n = 26) water buffalo females. CONCLUSIONS: Our results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards. [less ▲]

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See detailMonitoring the circadian rhythm of serum and salivary cortisol concentrations in the horse
Bohak, Zs; Szabo, F.; Beckers, Jean-François ULg et al

in Domestic Animal Endocrinology (2013), 45(1), 38-42

Daily fluctuations of cortisol concentration in the blood or saliva have been repeatedly reported. However, several contradictions in the existing literature appear on this subject. The present study was ... [more ▼]

Daily fluctuations of cortisol concentration in the blood or saliva have been repeatedly reported. However, several contradictions in the existing literature appear on this subject. The present study was performed to definitively establish options for testing adrenocortical function. To the best of our knowledge, this is the first study to evaluate parallel circadian rhythms in salivary and serum cortisol concentrations during a 24-h period. Twenty horses were examined under the same conditions. Blood and saliva samples were taken every 2 h for 24 h to determine the daily changes in cortisol concentrations of saliva and serum at rest and to determine the relationship between salivary and serum cortisol levels. Cosinor analysis of group mean data confirmed a significant circadian component for both serum and salivary cortisol concentrations (P < 0.001 in both cases). The serum cortisol circadian rhythm had an acrophase at 10:50 AM (95% CI, 10:00 AM-11:40 AM), a MESOR of 22.67 ng/mL, and an amplitude of 11.93 ng/mL. The salivary cortisol circadian rhythm had an acrophase at 10:00 AM (95% CI, 9:00 AM-11:00 AM), a MESOR of 0.52 ng/mL, and an amplitude of 0.12 ng/mL. We found a significant but weak association between salivary and serum cortisol concentrations; the Pearson correlation coefficient was 0.32 (P < 0.001). The use of salivary cortisol level as an indicator of hypothalamic-pituitary-adrenal axis activity may be warranted. However, the salivary cortisol levels are more likely to be correlated with free plasma cortisol than with the total plasma cortisol concentration. [less ▲]

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See detailRelationship between plasma progesterone and pregnancy-associated glycoprotein concentrations during early pregnancy in dairy cows
Barbato, O; Merlo, M; Celi, P et al

in Veterinary Journal (2013)

The relationship between the concentration of plasma progesterone (P4) during embryo attachment or at recognition of pregnancy, and that of pregnancy-associated glycoprotein (PAG) was assessed in dairy ... [more ▼]

The relationship between the concentration of plasma progesterone (P4) during embryo attachment or at recognition of pregnancy, and that of pregnancy-associated glycoprotein (PAG) was assessed in dairy cows. The outcome of artificial insemination (AI) was classified as positive (AI+), negative (AIÿ), or late embryonic mortality (EM) by measuring circulating PAG concentrations and by ultrasonography. Based on P4 concentrations at either day 21 or day 15, AI+ and EM cows were classified into ‘low’ (P4 concentrations < mean) and ‘high’ (P4 concentrations > mean) P4 groups. In both experiments, the threshold of P4 concentration between the ‘low’ and ‘high’ groups was approximately 6 ng/mL. PAG concentrations were lower in the ‘low’ group only when P4 concentrations were below the threshold. The study findings suggest that a possible P4 threshold exists below which PAG secretion may be impaired [less ▲]

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See detailEffect of Azawak colostrum administration on plasma protein profile in red kid
Abdoul, H; Marichatou, H; Beckers, Jean-François ULg et al

in Proceedings of the 2nd Scientific Meeting of the Faculty of Veterinary Medicine (2012, October 19)

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See detailPlacental and gastric aspartic proteinases: new insights from bovine species
Bella, Amina ULg; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg

in Proceedings of the 2nd Scientific Meeting of the Faculty of Veterinary Medicine (2012, October 19)

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See detailImmature equine oocyte vitrification
Gatez, Carine ULg; Ectors, F.J.; Farnir, Frédéric ULg et al

Scientific conference (2012, October 19)

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See detailEffect of bovine Azawak colostrum administration on plasma protein profile in red kid.
Abdou, Harouna ULg; Marichatou, Hamani; Beckers, Jean-François ULg et al

Poster (2012, October 11)

This study evaluated the impact of heterologous Azawak colostrum administration on plasma protein profile in red kids from Niger. Forty (40) newborn kids were affected to one of two treatments: control ... [more ▼]

This study evaluated the impact of heterologous Azawak colostrum administration on plasma protein profile in red kids from Niger. Forty (40) newborn kids were affected to one of two treatments: control group (access to water and the mother) and colostrum group (access to the mother, water and additional 50 ml of colostrum Azawak/animal the day of birth, then 25ml/animal/day from the 2nd to 15th day). Blood samples (10ml/animal) were obtained at the age of 10 and 30 days by jugular puncture into EDTA vacutainer tubes. The quantification of total protein was performed by the Biuret method. The agarose gel electrophoresis was used to determine serum levels of albumin, ɑ-globulin, β1-globulin, β2-globulin, γ-globulin and finally the albumin/globulin ratio. In general, the average concentrations of these proteins obtained at both ages reached higher values in colostrum than in control group. At day 10, the colostrum group tended to show (P <0.07) higher levels for ɑ-globulin and showed higher values (P <0.04) for β1-globulin. At day 30, total protein and β2-globulins were higher in colostrum group. The administration of heterologous colostrum in kid seems to have positive effects on some plasma parameters. It would be worth to discriminate the plasma proteins derived from bovine and maternal colostra. [less ▲]

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See detailCharacterisation of pregnancy losses after embryo transfer by measuring plasma progesterone and bovine pregnancy-associated glycoprotein-1 concentrations
Breukelman, S. P.; Perényi, Z.; Taverne, M. A. M. et al

in Veterinary Record : Journal of the British Veterinary Association (2012), 194(1), 71-76

The aim of this analysis was to determine whether pregnancy loss (PL) after embryo transfer (ET) in cattle was related to maternal progesterone (P4) concentrations during and shortly after ET, and ... [more ▼]

The aim of this analysis was to determine whether pregnancy loss (PL) after embryo transfer (ET) in cattle was related to maternal progesterone (P4) concentrations during and shortly after ET, and maternal bovine pregnancy-associated glycoprotein-1 (bPAG-1) concentrations in plasma at days 25–35 of gestation. Embryos (n = 260) were produced either in vivo after superovulation (n = 115), or in vitro from oocytes (obtained with ovum pick-up) in co-culture (n = 44) or cultured in a synthetic medium (n = 101). Overall, PL was 56.9% (148) and no significant differences occurred in calving rate among the three embryo production groups. There was no difference in P4 concentrations on days 7–14 of gestation in the three groups, nor between ongoing and interrupted pregnancies. Between days 25 and 35 of pregnancy, bPAG-1 concentrations were unaffected by embryo production, but in cattle that had PL between days 26 and 120, four bPAG-1 profiles could be detected. Between days 25 and 32, bPAG-1 concentrations were influenced by PL, and concentrations were significantly lower in animals in which PL occurred between days 26 and 120 than in those animals that aborted later or calved at term. Early P4 concentrations suggested that maternal luteal factors were not responsible for PL which appeared to be caused by impaired conceptus development (regardless of embryo type) as reflected by low maternal bPAG-1 concentrations prior to embryonic death [less ▲]

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See detailAssessment of pregnancy-associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera.
Nguyen, V. H.; Barbato, O.; Bui, X. N. et al

in Animal Science Journal (2012), 83(10), 683-689

Pregnancy-associated glycoproteins (PAG) constitute a large family of glycoproteins found in the outer placental epithelial cell layer of the placenta in Eutherian species. In ruminants, they are noted to ... [more ▼]

Pregnancy-associated glycoproteins (PAG) constitute a large family of glycoproteins found in the outer placental epithelial cell layer of the placenta in Eutherian species. In ruminants, they are noted to be structurally closely related among the different species. This study was designed to determine PAG concentrations in maternal and fetal plasma, allantoic and amniotic fluids in buffalo species. Antisera (AS) generated in rabbits against distinct PAG molecules were used in three RIA-PAG systems: RIA-1 (raised against bovine PAG67kDa; AS#497), RIA-2 (raised against caprine PAG55+62kDa; AS#706) or RIA-3 (raised against buffalo PAG; AS#859). Samples were collected at slaughterhouse (n = 67). PAG concentrations determined by RIA-2 gave significantly higher results in both allantoic and amniotic fluids (12.7 ± 2.1 ng/ml and 24.0 ± 7.3 ng/ml, respectively). Regarding maternal and fetal plasma, PAG concentrations obtained by RIA-2 (21.8 ± 2.4 ng/ml and 20.2 ± 2.5 ng/ml, respectively) and RIA-3 (25.0 ± 2.2 ng/mL and 21.9 ± 3.2 ng/ml, respectively) were higher than those obtained by RIA-1 (15.5 ± 1.4 ng/ml and 16.1 ± 1.8 ng/ml, respectively). The correlation among the three systems was very high. The study clearly reveals the ability of PAG-RIA systems to determine PAG concentration in swamp buffalo samples. [less ▲]

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See detailIn vitro oocyte IGF-I priming increases inner cell mass proliferation of in vitro-formed bovine blastocysts
Velazquez, MA; Hadeler, KG; Herrmann, D et al

in Theriogenology (2012), 78(3), 517-527

Studies addressing the effects of supraphysiological levels of IGF-1 on oocyte developmental competence are relevant for unravelling conditions resulting in high bioavailability of IGF-1, such as the ... [more ▼]

Studies addressing the effects of supraphysiological levels of IGF-1 on oocyte developmental competence are relevant for unravelling conditions resulting in high bioavailability of IGF-1, such as the polycystic ovary syndrome (PCOS). This study investigated the effects of supraphysiological levels of IGF-1 during in vivo folliculogenesis on the morula-blastocyst transition in bovine embryos. Compacted morulae were non-surgically collected and frozen for subsequent mRNA expression analysis (IGF1R, IGBP3, TP53, AKT1, SLC2A1, SLC2A3, and SLC2A8), or underwent confocalmicroscopy analysis for protein localization (IGF1R and TP53), or were cultured in vitro for 24 h. In vitro-formed blastocysts were subjected to differential cell staining. The mRNA expression of SLC2A8 was higher in morulae collected from cows treated with IGF-1. Both IGF1R and TP53 protein were present in the plasma membrane and cytoplasm. IGF-1 treatment did not affect protein localization of both IGF1R and TP53. In vitro-formed blastocysts derived from morulae recovered from IGF-1-treated cows displayed a higher number of cells in the inner cell mass (ICM). Total cell number (TCN) of in vitro-formed blastocysts was not affected. A higher mean ICM/TCN proportion was observed in in vitro-formed blastocysts derived from morulae collected from cows treated with IGF-1. The percentage of in vitro-formed blastocysts displaying a low ICM/TCN proportion was decreased by IGF-1 treatment. In vitro-formed blastocysts with a high ICM/TCN proportion were only detected in IGF-1 treated cows. Results show that even a short in vivo exposure of oocytes to a supraphysiological IGF-1 microenvironment can increase ICM cell proliferation in vitro during the morula to blastocyst transition [less ▲]

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See detailPregnancy-associated glycoprotein, chymosin and pepsinogen immunoreactivity of proteins extracted from fetal gastric tissue in bovine species.
Bella, Amina ULg; Melo de Sousa, Noelita ULg; Dehimi, M. L. et al

in Research in Veterinary Science (2012), 92(3), 378-386

The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical ... [more ▼]

The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical biochemical protocols were analyzed by proteolytic method, by PAG-RIA and by Western blot with the use of antisera raised against both pepsinogens and PAG. A strong reaction of proteins extracted from the fundic mucosa collected at the beginning of pregnancy was revealed with both anti-bPAG-I and anti-bPAG-II antisera, suggesting the expression of pepsinogen F in bovine species. Concerning pyloric mucosa, the analysis by Western blot highlighted a very strong immunoreaction with the anti-bovine chymosin serum. Amino-terminal sequencing allowed to identify bovine fetuin and albumin in fundic extracts, chymosin in the pyloric mucosa extracts, as well as some unknown proteins in both mucosa. Despite no N-terminal microsequence corresponding to the hypothetical pepsinogen F could be identified, it cannot be excluded that an existing bovine pepsinogen F-like molecule could be degraded during the purification procedure or that co-purified proteins could be responsible for masking its N-terminal microsequence. [less ▲]

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See detailThe inseminating bull and plasma pregnancy-associated glycoprotein levels were related to peripheral leukocyte counts during the late pregnancy/early postpartum period in high-producing dairy cows.
Abdelfatah-Hassan, A.; Almeria, S.; Serrano, B. et al

in Theriogenology (2012), 77(7), 1390-1397

The interest in the immunology and endocrinology aspects of the peripartum in dairy cows has been increasing in the last years, since the animal status during the pre- and postpartum period determines the ... [more ▼]

The interest in the immunology and endocrinology aspects of the peripartum in dairy cows has been increasing in the last years, since the animal status during the pre- and postpartum period determines the subsequent productive and reproductive performance of the animal. At parturition, a drop in the immune functions of the peripheral blood polymorph-nuclear cells (PMN) was observed after a peak of Pregnancy-Associated Glycoproteins (PAGs) and recently, the inseminating bull was found to affect the plasma levels of bovine Pregnancy-Associated Glycoproteins (bovine PAGs) in the pregnant Holstein-Friesian dairy cows. The present work was aimed to determine whether changes in leukocyte counts during the peripartum, indicative of the animal immune status, could be related to the inseminating bull and to the levels of plasma pregnancy-associated glycoproteins (PAGs). Ninety six clinically healthy, single pregnant cows were selected in a commercial dairy herd. Six blood samples (four prepartum and two postpartum) were obtained every two weeks, and total and differential blood cell counts were analyzed. Based on procedures of General Linear Model analysis of variance for repeated measures, the inseminating bull affected counts of total leukocytes and lymphocytes (P<0.001; between-subject effects) throughout the peripartum period. In addition, cows with high plasma levels of PAGs (>900 ρg/µL) on Day 262-268 of gestation had higher numbers of total leukocytes and neutrophils throughout the peripartum period (P<0.001; between-subject effects). Young animals (≤ 1 lactation) had higher total leukocyte and lymphocytes counts than older cows (two or more lactations) throughout the study period. These results showed a clear relationship between the inseminating bull and plasma PAG levels and peripheral leukocyte counts during the peripartum period of dairy cows. [less ▲]

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See detailInvestigations sur les protéines placentaires extraites des cotylédons fœtaux du Cervus elaphus
Fondja, E.; Okuyama, M.; Petrova, A. et al

Poster (2012, February)

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