References of "Beckers, Jean-François"
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See detailDetermination of minimum effective doses of luteinizing hormone and human chorionic gonadotropin for intrafollicular treatment to induce ovulation in dairy heifers.
Mala, J; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Acta Veterinaria BRNO (2013), 82

The aim of this study was to determine the minimum effective intrafollicular doses of luteinizing hormone and human chorionic gonadotropin in order to induce ovulation in cycling dairy heifers that have ... [more ▼]

The aim of this study was to determine the minimum effective intrafollicular doses of luteinizing hormone and human chorionic gonadotropin in order to induce ovulation in cycling dairy heifers that have not yet been adequately established. Application of 10, 5, 1, 0.5, 0.1, 0.01 and 0.001 µg luteinizing hormone as well as 10, 1, 0.1, 0.01 and 0.001 international units (IU) of human chorionic gonadotropin in dominant follicles was performed on day 7 of the oestrous cycle. Control animals were given luteinizing hormone (12.5 mg and 25 mg) or human chorionic gonadotropin (2000 IU) intravenously. Accessory corpus luteum on day 14 of the oestrous cycle was considered as an evidence of ovulation. Ovulation was observed in 2 out of 3 heifers in each treatment group (n = 3) after administration of 10–0.1 µg luteinizing hormone (except for 0.5 µg – ovulation in 3 of 3 heifers), in all heifers after administration of 10–0.01 IU human chorionic gonadotropin as well as in all control heifers. Administration of 0.01 µg and 0.001 µg luteinizing hormone as well as of 0.001 IU human chorionic gonadotropin did not result in ovulation. Higher progesterone concentration on day 14 vs. day 7 of the oestrous cycle was found after all treatments. Nevertheless, the differences were signicant (P < 0.05) only after intrafollicular treatments with 5, 1 and 0.001 µg luteinizing hormone as well as 10, 1 and 0.01 IU human chorionic gonadotropin. In conclusion, minimum efcient doses for intrafollicular treatment of the dominant follicles in cycling heifers capable of inducing ovulation were 0.1 µg of luteinizing hormone and 0.01 IU of human chorionic gonadotropin. This is the rst study describing the intrafollicular luteinizing hormone administration in cycling dairy heifers. [less ▲]

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See detailIntrafollicular LH administration in dairy heifers treated with a GnRH agonist
Mala, J; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Veterinarni Medicina (2013), 58(2), 8186

The aim of this study was to evaluate the effect of intrafollicular treatment (IFT) with different doses of luteinising hormone. Experimental heifers were treated with a single deslorelin implant to ... [more ▼]

The aim of this study was to evaluate the effect of intrafollicular treatment (IFT) with different doses of luteinising hormone. Experimental heifers were treated with a single deslorelin implant to desensitise gonadotroph cells of the pituitary gland. Thereafter, follicular development was stimulated by exogenous FSH treatment. Intrafollicular treatment with 10, 5, 1 and 0.01 μg LH was performed on one single follicle while other follicles remained untreated. Human chorionic gonadotrophine (2000 UI) was administered intravenously as a control. Ovulation and development of the corpus luteum occurred after all intrafollicular treatments with 10 and 5 μg LH. After IFT using 1 μg of LH 75% animals (3/4) ovulated. The dose of 0.01 μg was not followed by any ovulation whereas control treatments with hCG were followed by an ovulation of the majority of follicles present in the ovaries. In conclusion, IFT with different doses of LH (greater than 0.01 μg) is capable of inducing ovulation. [less ▲]

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See detailProfiles of pregnancy-associated glycoprotein (PAG) concentrations during gestation in swamp buffalo
Van Hahn, N; Viet Linh, N; Melo de Sousa, Noelita ULg et al

in Proceedings of the 10th World Buffalo Congress and 7th Asian Buffalo Congress (2013)

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See detailPregnancy-Associated Glycoproteins (PAGs) in prolific (Boujaâd x D’man) and non- prolific (Boujaâd) Moroccan sheep by means of four radio-immunoassays
El Amiri, Bouchra; Melo de Sousa, Noelita ULg; El Abbadi, N et al

in Proceedings of the 23rd International Congress of Hungarian Association for Buiatrics (2013)

The aim of the present study was: i) to investigate the use of PAGs for pregnancy follow up in Boujaâd non prolific (n=8) vs. Boujaâd x D’man a prolific sheep (n=20), ii) to study whether the boPAG tracer ... [more ▼]

The aim of the present study was: i) to investigate the use of PAGs for pregnancy follow up in Boujaâd non prolific (n=8) vs. Boujaâd x D’man a prolific sheep (n=20), ii) to study whether the boPAG tracer (daily available at the laboratory of physiology of reproduction, Liège, could be used in RIA without affecting the results, iii) to prospect the possibility of transferring the technique to a Moroccan laboratory. The blood samples were weekly collected from the first week of gestation till the 4 week after lambing. The RIA1, RIA2, RIA3 and RIA4 were all based on ovPAG standard, while the tracer and antiserum changed according to the system. The RIA1, RIA3 and RIA4 were performed in Belgium while the RIA2 was carried out in Morocco. The results showed that the highest correlation was obtained between the RIA3 and RIA4 (R2 = 0.83) while the lowest was recorded between the RIA2 and RIA4 (R2 = 0.69). The lowest concentrations derived from the RIA2. The highest concentrations were recorded before lambing (279.29; 88.21; 268.75; 152.67 ng/ml respectively for RIAs 1, 2, 3 and 4). To conclude, the profiles of ovPAG showed that it is possible to flow-up with success using the four systems. In addition, the RIA1 using boPAG as tracer could be a good alternative to the homologous one and finally, it is possible to set up routinely the RIA technique for PAG detection in Moroccan laboratories. [less ▲]

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See detailPregnancy-associated glycoproteins in sheep: breeds, techniques and achievements
El Amiri, Bouchra; Melo de Sousa, Noelita ULg; Szenci, Otto et al

in Proceedings of the 23rd International Congress of Hungarian Association for Buiatrics (2013)

The aim of the present review is to summarize the achievements on pregnancy-associated glycoproteins in sheep during the last 20 years on terms of results obtained after purifications, molecular biology ... [more ▼]

The aim of the present review is to summarize the achievements on pregnancy-associated glycoproteins in sheep during the last 20 years on terms of results obtained after purifications, molecular biology, radio-immunoassays (RIA), enzyme linked immune sorbent assay (ELISA), double radial immune-diffusion (Ouchterlony) and Western blot techniques. The analysis of documented papers that concerned these attempts showed that the animal genetic material used was composed of variable breeds from north Africa, Europe and USA (the Aragonesa, Ouled Djellal, Laucaun drairy sheep, Berrichone, Texel x Norvegian crossed ewes, Awassi x Merino, Blackheaded Moutton sheep x Blackheaded German Mutton sheep (BGM), Rhoen sheep crosses of BGM with Dorper sheep, Assaf, Chura, Merino, Texel, Suffolk, Boujaâd Moroccan breed). The RIA represents the wildspread technique used (54%) while Oucherlony and Western blot had been limited in use (4% each). From 1990 to 2000 only 27% of the achievements being available today were realized. The main data, profiles and studies were performed from 2000 to now (63%). To conclude, this review allows summarizing and updating the knowledge about the PAG in sheep. [less ▲]

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See detailSerum albumin [Fragment] from Dama dama placenta - Accession number C0HJD2
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailAlpha-2-HS-glycoprotein [Fragment] from Dama dama placenta - Accession number C0HJD1
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein D-56 kDa [Fragment] from Dama dama placenta - Accession number C0HJD0
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein C-39 kDa [Fragment] from Dama dama placenta - Accession number C0HJC9
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein B-56 kDa [Fragment] from Dama dama placenta - Accession number C0HJC8
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein A-62 kDa [Fragment] from Dama dama placenta - Accession number C0HJC7
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-associated glycoprotein (PAG) pattern and pregnancy detection in Boer goats using an ELISA with different antisera
Shahin, M; Friedrich, M; Gauly, M et al

in Small Ruminant Research (2013), 113

Pregnancy-associated glycoproteins (PAGs) are macromolecules produced by placental tissue and released into the maternal circulation where they allow pregnancy diagnosis and follow-up. The present study ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) are macromolecules produced by placental tissue and released into the maternal circulation where they allow pregnancy diagnosis and follow-up. The present study addresses the question to what extent plasma PAG determination may serve as a means of early pregnancy detection in goats in a similar way it is practiced in cows, and whether an ovine or bovine PAG-ELISA may be utilized to this end. Blood samples were collected from eight pregnant pluriparous Boer goat does twice weekly during the first seven seeks and the last four weeks of pregnancy and weekly in-between and during four weeks following parturition. Plasma PAG concentrations (mean±SEM) were determined using a competitive enzyme-linked immunosorbent assay. Assays were conducted with polyclonal antisera raised in rabbits against purified preparations of caprine (AS#706), ovine (AS#780) and bovine PAG (AS#726). In the assay systems purified bovine PAG served as standard and tracer and goat anti-rabbit IgG served as coating antibody. With the antibody raised against caprine PAG (AS#706) a steep increase to a climax of 69±9 ng/ml on day 56 of pregnancy was followed by a gradual decline to 16±3 ng/ml at parturition and 0.3±0.07 ng/ml four weeks postpartum. The results achieved with the antiovine PAG (AS#780) showed close similarity, a maximum of 92±14 ng/ml being reached at 56 days of pregnancy. With anti-bovine PAG (AS#726), the PAG level increased to a maximum of 3.1±0.2 ng/ml on day 105 of pregnancy and fluctuated around 3 ng/ml until the end of pregnancy. The difference between pregnant and non-pregnant does reached a significant level 21 days after conception, one week earlier than with caprine and ovine antisera. [less ▲]

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See detailRider and horse salivary cortisol levels during competition and impact on performance.
Peeters, Marie ULg; Closson, Coline; Beckers, Jean-François ULg et al

in Journal of Equine Veterinary Science (2013), 33(3), 155-160

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See detailPlasma concentrations of pregnancy-associated glycoproteins measured using anti-bovine PAG-2 antibodies on Day 120 of gestation predict abortion in dairy cows naturally infected with Neospora caninum.
Garcia-ispierto, I; Almeria, S; Serrano, B et al

in Reproduction in Domestic Animals (2013), 48

The present study sought to determine: (i) the effects of Neospora caninum infection and twin pregnancy on plasma pregnancy-associated glycoprotein-2 (PAG-2) concentrations throughout pregnancy and (ii ... [more ▼]

The present study sought to determine: (i) the effects of Neospora caninum infection and twin pregnancy on plasma pregnancy-associated glycoprotein-2 (PAG-2) concentrations throughout pregnancy and (ii) whether plasma PAG-2 concentrations could predict abortion in N. caninum-infected cows. The study was performed on a commercial Holstein-Friesian dairy herd in northeastern Spain and the final data included those recorded in 53 non-aborting and 19 aborting animals. Blood samples were collected immediately before pregnancy diagnosis (on Days 40, 90, 120, 150, 180 and 210 post-insemination) in non-aborting cows or until the time of abortion detection in aborting cows. General lineal models (GLM) repeated measures ANOVA revealed the different behavior of PAG-1 and PAG-2, and significant effects of Neospora seropositivity, cool season and twin pregnancy on plasma PAG-2 concentrations throughout gestation (between-subject effects). In addition, based on the odds ratios, the likelihood of abortion increased in Neospora-seropositive cows (by a factor of 7.0) compared to seronegative animals and decreased in cows with a high plasma PAG-2 concentration (>4.5 ng/ml) on Day 120 of pregnancy (by a factor of 0.24), compared to the remaining cows. In conclusion, there is a relationship between plasma PAG-2 concentrations and the risk of abortion in Neosporainfected dairy cows. Thus, plasma PAG concentrations measured using anti-boPAG-2 antiserum on Day 120 of gestation could serve as an indicator of the abortion risk in N. caninum infected animals; values <4.5 ng/ml indicating a high risk of abortion in chronically infected animals. [less ▲]

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See detailPurification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females.
Barbato, Olimpia; Melo de Sousa, Noelita ULg; Barile, Vittoria Lucia et al

in BMC Veterinary Research (2013), 9

BACKGROUND: Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of ... [more ▼]

BACKGROUND: Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species. RESULTS: Amino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n = 33) and non-pregnant (n = 26) water buffalo females. CONCLUSIONS: Our results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards. [less ▲]

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See detailMonitoring the circadian rhythm of serum and salivary cortisol concentrations in the horse
Bohak, Zs; Szabo, F.; Beckers, Jean-François ULg et al

in Domestic Animal Endocrinology (2013), 45(1), 38-42

Daily fluctuations of cortisol concentration in the blood or saliva have been repeatedly reported. However, several contradictions in the existing literature appear on this subject. The present study was ... [more ▼]

Daily fluctuations of cortisol concentration in the blood or saliva have been repeatedly reported. However, several contradictions in the existing literature appear on this subject. The present study was performed to definitively establish options for testing adrenocortical function. To the best of our knowledge, this is the first study to evaluate parallel circadian rhythms in salivary and serum cortisol concentrations during a 24-h period. Twenty horses were examined under the same conditions. Blood and saliva samples were taken every 2 h for 24 h to determine the daily changes in cortisol concentrations of saliva and serum at rest and to determine the relationship between salivary and serum cortisol levels. Cosinor analysis of group mean data confirmed a significant circadian component for both serum and salivary cortisol concentrations (P < 0.001 in both cases). The serum cortisol circadian rhythm had an acrophase at 10:50 AM (95% CI, 10:00 AM-11:40 AM), a MESOR of 22.67 ng/mL, and an amplitude of 11.93 ng/mL. The salivary cortisol circadian rhythm had an acrophase at 10:00 AM (95% CI, 9:00 AM-11:00 AM), a MESOR of 0.52 ng/mL, and an amplitude of 0.12 ng/mL. We found a significant but weak association between salivary and serum cortisol concentrations; the Pearson correlation coefficient was 0.32 (P < 0.001). The use of salivary cortisol level as an indicator of hypothalamic-pituitary-adrenal axis activity may be warranted. However, the salivary cortisol levels are more likely to be correlated with free plasma cortisol than with the total plasma cortisol concentration. [less ▲]

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See detailRelationship between plasma progesterone and pregnancy-associated glycoprotein concentrations during early pregnancy in dairy cows
Barbato, O; Merlo, M; Celi, P et al

in Veterinary Journal (2013)

The relationship between the concentration of plasma progesterone (P4) during embryo attachment or at recognition of pregnancy, and that of pregnancy-associated glycoprotein (PAG) was assessed in dairy ... [more ▼]

The relationship between the concentration of plasma progesterone (P4) during embryo attachment or at recognition of pregnancy, and that of pregnancy-associated glycoprotein (PAG) was assessed in dairy cows. The outcome of artificial insemination (AI) was classified as positive (AI+), negative (AIÿ), or late embryonic mortality (EM) by measuring circulating PAG concentrations and by ultrasonography. Based on P4 concentrations at either day 21 or day 15, AI+ and EM cows were classified into ‘low’ (P4 concentrations < mean) and ‘high’ (P4 concentrations > mean) P4 groups. In both experiments, the threshold of P4 concentration between the ‘low’ and ‘high’ groups was approximately 6 ng/mL. PAG concentrations were lower in the ‘low’ group only when P4 concentrations were below the threshold. The study findings suggest that a possible P4 threshold exists below which PAG secretion may be impaired [less ▲]

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See detailEffect of Azawak colostrum administration on plasma protein profile in red kid
Abdoul, H; Marichatou, H; Beckers, Jean-François ULg et al

in Proceedings of the 2nd Scientific Meeting of the Faculty of Veterinary Medicine (2012, October 19)

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See detailPlacental and gastric aspartic proteinases: new insights from bovine species
Bella, Amina ULg; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg

in Proceedings of the 2nd Scientific Meeting of the Faculty of Veterinary Medicine (2012, October 19)

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See detailImmature equine oocyte vitrification
Gatez, Carine ULg; Ectors, F.J.; Farnir, Frédéric ULg et al

Scientific conference (2012, October 19)

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