References of "Beckers, Jean-François"
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See detailPregnancy patterns during the early fetal period in high producing dairy cows treated with GnRH or progesterone.
Bech-Sabat, G.; Lopez-Gatius, F.; Garcia-Ispierto, I. et al

in Theriogenology (2009), 71

In order to explore pregnancy patterns in high producing dairy cows treated with GnRH or progesterone at pregnancy diagnosis (Days 28-34), two consecutive experiments were designed. In Experiment 1, cows ... [more ▼]

In order to explore pregnancy patterns in high producing dairy cows treated with GnRH or progesterone at pregnancy diagnosis (Days 28-34), two consecutive experiments were designed. In Experiment 1, cows bearing a single embryo were randomly assigned to a PRID (n=40; cows fitted with a progesterone releasing intra-vaginal device for 28 days), GnRH (n=40; cows receiving GnRH) or Control (n=26; untreated cows) group. PRID treatment led to a rise in plasma progesterone concentrations in the 7 days following the onset of treatment compared to the other two groups. In Experiment 2, in which we also examined twin pregnancies, animals were randomly assigned to PRID (n=312) or GnRH (n=294) treatment groups. Treatments were the same as described for Experiment 1. Logistic regression procedures revealed that in cows with a single corpus luteum, the probability of pregnancy loss between the first (Days 28-34) and second (Days 65-62) pregnancy diagnosis decreased by a factor of 0.51 in the PRID group compared to the GnRH group. However, in cows with two or more corpora lutea, PRID treatment increased the likelihood of pregnancy loss by a factor of three, compared to GnRH treatment. In cows carrying twins, the conceptus reduction rate was higher (P=0.02) for the GnRH (36%) than for the PRID (16.4%) group. Formation of a new corpus luteum was recorded in 17.7% of cows in the GnRH group. Our results indicate that compared to GnRH treatment, progesterone treatment given at pregnancy diagnosis in high producing dairy cows, reduced by a factor of 0.51 and increased by a factor of 3 the probability of pregnancy loss in cows with a single or with two or more corpora lutea, respectively, and reduced the conceptus reduction rate in cows carrying twins. The practical implications of our findings are that in herds with a high incidence of early fetal loss of a non-infectious nature, treatment at the time of pregnancy diagnosis with PRID in cows with one corpus luteum and with GnRH in cows with two or more corpora lutea should offer considerable benefits. [less ▲]

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See detailFirst birth of an animal from an extinct subspecies (Capra pyrenaica pyrenaica) by cloning
Folch, J.; Cocero, M. J.; Chesne, P. et al

in Theriogenology (2009), 71

Two experiments have been performed to clone the bucardo, an extinct wild goat. The karyoplasts were thawed fibroblasts derived from skin biopsies, obtained and cryopreserved in 1999 from the last living ... [more ▼]

Two experiments have been performed to clone the bucardo, an extinct wild goat. The karyoplasts were thawed fibroblasts derived from skin biopsies, obtained and cryopreserved in 1999 from the last living specimen, a female, which died in 2000. Cytoplasts were mature oocytes collected from the oviducts of superovulated domestic goats. Oocytes were enucleated and coupled to bucardo's fibroblasts by electrofusion. Reconstructed embryos were cultured for 36h or 7d and transferred to either Spanish ibex or hybrid (Spanish ibex malex domestic goat) synchronized recipients. Embryos were placed, according to their developmental stage, into the oviduct or into the uterine horn ipsilateral to an ovulated ovary. Pregnancy was monitored through their plasmatic PAG levels. In Experiment 1, 285 embryos were reconstructed and 30 of them were transferred at the 3- to 6-cells stage to 5 recipients. The remaining embryos were further cultured to day 7, and 24 of them transferred at compact morula/blastocyst stage to 8 recipients. In Experiment 2, 154 reconstructed embryos were transferred to 44 recipients at the 3- to 6-cells stage. Pregnancies were attained in 0/8 and 7/49 of the uterine and oviduct-transferred recipients, respectively. One recipient maintained pregnancy to term, displaying very high PAG levels. One morphologically normal bucardo female was obtained by caesarean section. The newborn died some minutes after birth due to physical defects in lungs. Nuclear DNA confirmed that the clone was genetically identical to the bucardo's donor cells. To our knowledge, this is the first animal born from an extinct subspecies. [less ▲]

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See detailGamma interferon production correlates negatively with plasma levels of pregnancy-associated glycoprotein-1 (PAG-1) during gestation in dairy cows naturally infected with Neospora caninum
Lopez-Gatius, F.; Almeria, S.; Yániz, J. L. et al

in O’Leary, M.; Arnett, J. (Eds.) Pregnancy Protein Research (2009)

Gamma interferon (IFN-γ) production has been ascribed a role in protecting cows infected with Neospora caninum against abortion. The present study analyzes the interaction between IFN-γ production and ... [more ▼]

Gamma interferon (IFN-γ) production has been ascribed a role in protecting cows infected with Neospora caninum against abortion. The present study analyzes the interaction between IFN-γ production and levels of plasma pregnancy-associated glycoprotein-1 (PAG-1), as a marker of placental/fetal well-being, throughout gestation in naturally Neospora-infected dairy cows. Data were obtained from 88 pregnant cows from two herds: 62 seropositive and 26 seronegative for the parasite. Blood sample collection was performed on days 40, 90, 120, 150, 180 and 210 of gestation. Plasma was tested for antibodies against N. caninum, PAG-1 and IFN-γ. Twenty five (28.4%) pregnancies were recorded after AI using Holstein-Friesian semen (19 in seronegative and 6 in seropositive animals), and 63 (71.6%) after AI using Limousin semen (7 in seronegative and 56 in seropositive animals). Gamma interferon was detected in the plasma of 14 (22.6%) of the 62 Neospora-seropositive cows and could not be detected in any of the 26 seronegative animals. All 14 cows producing IFN-γ became pregnant by using Limousin semen. Our GLM repeated measures showed no effect of herd, lactation number, milk production at the time of pregnancy diagnosis and Neospora-seropositivity on plasma PAG-1 concentrations. Significant positive effects of both the day of gestation (P<0.0001) and interaction between day of gestation and breed of sire (P = 0.001) on PAG-1 values were registered. Cows carrying twins had higher (P = 0.002) PAG-1 concentrations throughout gestation than cows carrying singletons. Interactions between breed of sire and Neospora-seropositivity (P<0.0001), and between IFN-γ production and Neospora-seropositivity (P = 0.04) were also detected. Thus, Neospora-seronegative cows inseminated with Limousin and Neospora-seropositive cows with no IFN-γ production, exhibited higher PAG-1 concentrations throughout gestation than seropositive cows inseminated with Limousin semen and producing IFN-γ, respectively. Our data indicate that production of IFN-γ correlates negatively and, the production of antibodies against N. caninum is uncorrelated with plasma PAG-1 concentrations during gestation in Neospora-infected dairy cows. Our results also suggest that augmented Th1 cell-mediated immunity is related to a lower risk of abortion and therefore higher resistance to the parasite in cows inseminated with Limousin versus Holstein-Friesian semen. [less ▲]

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See detailPregnancy-Associated Glycoprotein D-76 kDa [Fragment] from Bubalus bubalis placenta - Access number P86375
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein C-61 kDa [Fragment] from Bubalus bubalis placenta - Access number P86374
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein B-73 kDa [Fragment] from Bubalus bubalis placenta - Access number P86373
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein A-69 kDa [Fragment] from Bubalus bubalis placenta - Access number P86372
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein I-73 kDa [Fragment] from Bubalus bubalis placenta -Access number P86371
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein J-63 kDa [Fragment] from Bubalus bubalis placenta - Access number P86370
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein K-60 kDa [Fragment] from Bubalus bubalis placenta - Access number P83369
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailAnomalous Pregnancies during Late Embryonic/Early Foetal Period in High Producing Dairy Cows.
Serrano, B.; Lopez-Gatius, F.; Hunter, R. et al

in Reproduction in Domestic Animals (2009), 44(4), 672-676

This study analyses anomalous cases of gestation ending in pregnancy loss during the early foetal period and their effect on progesterone and plasma pregnancy-associated glycoprotein-1 (PAG-1 ... [more ▼]

This study analyses anomalous cases of gestation ending in pregnancy loss during the early foetal period and their effect on progesterone and plasma pregnancy-associated glycoprotein-1 (PAG-1) concentrations. Data derived from a large-scale ultrasound pregnancy diagnosis programme in high producing dairy cows. Over a 3-year period (2004-2007), a very low incidence (0.5%: 15 of 3094) of anomalous pregnancies was recorded. The results revealed that the following anomalies were detected on days 35-41 of gestation in cows carrying singletons with one single corpus luteum: embryo death in eight cows (0.3%); and embryo in the uterine horn contralateral to the corpus luteum in seven cows (0.2%). All these animals suffered pregnancy loss during the early foetal period. In cows carrying dead embryos, no signs of conceptus degeneration were observed on pregnancy diagnosis. Amnion size (approximately 25 mm diameter) and uterine horn fluid contents were estimated to be similar to those of the normal pregnant cows in this period. In the contralateral gestations, live embryos were observed in all ultrasound checks before pregnancy loss. Uterine fluid contents increased in the two cows in which gestation continued for more than a week. In the cases of embryo death but not in those of contralateral gestation, a drop in PAG-1 levels was noted prior to pregnancy loss. Two cows carrying dead embryos increased with time allantoic fluid contents. The PAG-1 values increased with time in one cow bearing a dead embryo (from 2.31 to 6.79 ng/ml) and in two of the contralateral gestations (from 1.66 to 2.33 ng/ml and from 0.39 to 6.79 ng/ml, respectively). Results of this study indicate that the foetal membranes continue to undergo some activity following embryo death, and that contralateral pregnancy may determine failure of the gestation process. [less ▲]

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See detailComparison of accuracy of ultrasonography, progesterone and pregnancy-associated 4 glycoprotein tests for pregnancy diagnosis in semi-domesticated reindeer
Savela, Hannele; Vahtiala, S.; Lindeberg, H. et al

in Theriogenology (2009), 72

The aim of the study was to compare transrectal ultrasound with progesterone (P4) and pregnancy-associated glycoproteins (PAG) as pregnancy detection methods for semi-domesticated reindeer in field ... [more ▼]

The aim of the study was to compare transrectal ultrasound with progesterone (P4) and pregnancy-associated glycoproteins (PAG) as pregnancy detection methods for semi-domesticated reindeer in field conditions. Female reindeer (n=195) were scanned transrectally by a 7.5 MHz linear array transducer and blood sampled either in Dec 2005 (n=33), Dec 2006 (n=92) or Jan 2007 (n=70) during early- or mid-gestation. Plasma levels of P4 and PAG were assessed by RIA. Based on calving records, the sensitivity, specificity, predictive values and the overall accuracy of the three tests were calculated. The overall calving rate calculated from the calving records was 86.2%. The overall accuracy of transrectal ultrasound was 99.5%. The sensitivity and specificity of transrectal ultrasound were 99.4% and 100%, respectively. In the plasma P4 test, the threshold level of 5.5 nmol/L gave the highest overall accuracy (93.3%). The sensitivity of the P4 test decreased from 96.4% to 81.5%, when the threshold level increased from 5.0 nmol/L to 8.0 nmol/L, while the specificity remained at 85.2% over the range of these cut-off values. The overall accuracy of the plasma PAG test decreased from 96.4% to 64.1% when the plasma PAG threshold level increased from 0.5 ng/mL to 3.5 ng/mL, whereas sensitivity decreased from 99.4% to 58.3%. Specificity increased from 77.8% to 100% when the plasma PAG threshold level reached 3.0 ng/mL. Transrectal ultrasound showed higher diagnostic values than plasma P4-RIA and PAG-RIA in diagnosing pregnancy of reindeer, with the advantage that diagnoses can be made in real-time in field conditions [less ▲]

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See detailAnti-mullerian hormone is an endocrine marker of ovarian gonadotropin-responsive follicles and can help to predict superovulatory responses in the cow.
Rico, Charlene; Fabre, Stephane; Medigue, Claire et al

in Biology of Reproduction (2009), 80(1), 50-59

The major limitation to the development of embryo production in cattle is the strong between-animal variability in ovulatory response to FSH-induced superovulation, mainly due to differences in ovarian ... [more ▼]

The major limitation to the development of embryo production in cattle is the strong between-animal variability in ovulatory response to FSH-induced superovulation, mainly due to differences in ovarian activity at the time of treatment. This study aimed to establish whether anti-Mullerian hormone (AMH) was an endocrine marker of follicular populations in the cow, as in human, and a possible predictor of the ovarian response to superovulation. Anti-Mullerian hormone concentrations in plasma varied 10-fold between cows before treatment and were found to be highly correlated with the numbers of 3- to 7-mm antral follicles detected by ovarian ultrasonography before treatment (r=0.79, P<0.001) and the numbers of ovulations after treatment (r=0.64, P<0.01). Between-animal differences in AMH concentrations were found to be unchanged after a 3-mo delay (r=0.87, P<0.01), indicating that AMH endocrine levels were characteristic of each animal on a long-term period. The population of healthy 3- to 7-mm follicles was the main target of superovulatory treatments, contained the highest AMH concentrations and AMH mRNA levels compared with larger follicles, and contributed importantly to AMH endocrine levels. In conclusion, AMH was found to be a reliable endocrine marker of the population of small antral gonadotropin-responsive follicles in the cow. Moreover, AMH concentrations in the plasma of individuals were indicative of their ability to respond to superovulatory treatments. [less ▲]

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See detailSerum Profiles of Pregnancy-Associated Glycoprotein, Oestrone Sulphate and Progesterone During Gestation and Some Factors Influencing the Profiles in Ethiopian Borana and Crossbred Cattle.
Lobago, F.; Bekana, M.; Gustafsson, H. et al

in Reproduction in Domestic Animals (2009), 44(4), 685-692

Contents This study presents serum concentrations of pregnancy-associated glycoprotein (PAG), oestrone sulphate (E1-S) and progesterone (P4), and the effects of some dam and foetus-related factors on ... [more ▼]

Contents This study presents serum concentrations of pregnancy-associated glycoprotein (PAG), oestrone sulphate (E1-S) and progesterone (P4), and the effects of some dam and foetus-related factors on these profiles during gestation in Borana and crossbred cattle. The PAG concentrations at 4th week post-conception ranged from 1.5-5.5 and 2.1-4.7 ng/ml in Borana (n = 6) and crossbred (n = 8) cattle, respectively. The mean PAG concentrations increased progressively from 4th to 33rd week of gestation (from 3.3-173 ng/ml for Borana and 4.2-240 ng/ml for crossbred cattle) and reached peak around calving. Breed, parity status, dam body weight, foetal sex and foetal birth weight significantly influenced the PAG concentrations. After delivery, the PAG concentrations declined steadily to 5.7 ng/ml in Borana (n = 7) and 3.9 ng/ml in crossbred (n = 6) cattle 10 weeks post-partum. The serum E1-S concentrations at 17th week of pregnancy ranged from 0.3-2.6 and 0.9-5.7 ng/ml in Borana (n = 8) and crossbred (n = 9) cattle, respectively. The mean E1-S concentrations increased progressively from 17th to 33rd week of gestation (from 1.1-4.6 ng/ml for Borana and 2.7-10.8 ng/ml for crossbred). Breed, parity status, dam body weight and foetal sex significantly influenced E1-S concentrations. The P4 concentrations at 4th week of pregnancy ranged from 3.2-5.1 and 1.7-8.9 ng/ml in Borana (n = 6) and crossbred (n = 8) cattle, respectively. The P4 level remained elevated throughout pregnancy. This study indicated that the serum PAG and P4 concentrations at 4th and E1-S approximately 17th week of pregnancy were above the cut-off value for pregnancy test and the hormonal profiles observed were comparable to the previous reports. Furthermore, the PAG and E1-S profiles were considerably influenced by factors such as breed, weight and parity status of the dam, and foetal sex and foetal birth weight (only PAG). [less ▲]

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See detailPregnancy-associated glycoproteins as diagnostic tool in cattle reproduction
Gajewski, Zdzislaw; Pertajitis; Melo de Sousa, Noelita ULg et al

in Schweizer Archiv für Tierheilkunde (2009), 151(12), 577-582

Pregnancy-associated glycoproteins are powerful pregnancy markers in domestic cattle. These proteins are expressed in mono- and binucleate trophoblast cells from the first days of gestation until calving ... [more ▼]

Pregnancy-associated glycoproteins are powerful pregnancy markers in domestic cattle. These proteins are expressed in mono- and binucleate trophoblast cells from the first days of gestation until calving. Different molecules were identified as being expressed at various stages of pregnancy. However, up to date, their functions and activities during pregnancy were not yet established. Specific RIA tests were developed (classic and alternative RIA) and used to measure the concentration of these glycoproteins in blood during gestation and the postpartum period in cattle. In maternal blood, PAGs rise to detectable levels from the 24th-28th days after fertilization. A recent study indicated that PAGs can also be detected in milk samples. However, concentrations in milk are much lower when compared to those of plasma. [less ▲]

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See detailCorrelation of five radioimmunoassay systems for measurement of bovine plasma pregnancy-associated glycoprotein concentrations at early pregnancy period.
Ayad, A.; Melo de Sousa, Noelita ULg; Sulon, Joseph ULg et al

in Research in Veterinary Science (2009), 86

The measurement of serum or plasma PAG concentrations is currently used as a specific method for pregnancy diagnosis in cattle. In this study, the correlation between five radioimmunoassay systems (RIA ... [more ▼]

The measurement of serum or plasma PAG concentrations is currently used as a specific method for pregnancy diagnosis in cattle. In this study, the correlation between five radioimmunoassay systems (RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool) developed for measurement of PAG concentrations in ruminant species was investigated in plasma from pregnant Friesian Holstein females. Plasma PAG concentrations (ng/mL) measured by different RIA systems were significantly correlated between them ( 0.81; P<0.001). PAG concentrations increased significantly from Day 21 (n=27) to 30 (n=37) after AI by use of all PAG-RIA systems. From Day 30 to 80 after AI, lower PAG concentrations were observed when using the homologous system RIA-497. The addition of several proteinase inhibitors changed neither the non specific binding nor the B(0) binding to the tracer. Our results suggest that all tested PAG-RIA (RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool) are highly correlated and can be useful to follow PAG concentrations in samples collected during the first trimester of gestation. [less ▲]

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See detailDetermination of sex and scrapie resistance genotype in preimplantation ovine embryos.
Guignot, F.; Baril, Gerard; Dupont, Francis et al

in Molecular Reproduction and Development (2009), 76(2), 183-190

The aim of this study was to test the accuracy of genotype diagnosis after pre-amplification of DNA extracted from biopsies obtained by microblade cutting of ovine embryos and to evaluate the viability of ... [more ▼]

The aim of this study was to test the accuracy of genotype diagnosis after pre-amplification of DNA extracted from biopsies obtained by microblade cutting of ovine embryos and to evaluate the viability of biopsied embryos after vitrification/warming and transfer to recipients. Sex and PrP genotypes were determined. Sex diagnosis was done by PCR amplification of ZFX/ZFY and SRY sequences after PEP-PCR while PrP genotype determination was performed after specific pre-amplification of specific target including codons 136, 154 and 171. Embryos were collected at Day 7 after oestrus. Blastocysts and expanded blastocysts were biopsied immediately after collection whereas compacted morulae were biopsied after 24 hr of in vitro culture. Eighty-nine biopsied embryos were frozen by vitrification. Fresh and vitrified whole embryos were kept as control. DNA of biopsies was extracted and pre-amplified. Sex diagnosis was efficient for 96.6% of biopsies and PrP genotyping was determined in 95.8% of codons. After embryo transfer, no significant difference was observed in lambing rate between biopsied, vitrified control and fresh embryos (54.5%, 60% and 66.6%, respectively). Embryo survival rate was not different between biopsied and whole vitrified embryos (P = 0.38). At birth, 96.7% of diagnosed sex and 95.4% of predetermined codons were correct. Lamb PrP profiles were in agreement with parental genotype. PEP-PCR coupled with sex diagnosis and nested PCR coupled with PrP genotype predetermination are very accurate techniques to genotype ovine embryo before transfer. These original results allow planning of selection of resistant genotype to scrapie and sex of offspring before transfer of cryopreserved embryo [less ▲]

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See detailConcentrations of pregnancy-associated glycoproteins in Water buffaloes females (Bubalus bubalis) during pregnancy and postpartum periods
Barbato, O.; Melo de Sousa, Noelita ULg; Malfatti, A. et al

in O’Leary, M.; Arnett, J. (Eds.) Pregnancy Protein Research (2009)

The concentrations of pregnancy-associated glycoproteins (PAG) were determined in buffalo cows (Bubalus bubalis) using three different radioimmunoassay (RIA) systems (RIA-497, RIA-706, and RIA-708 ... [more ▼]

The concentrations of pregnancy-associated glycoproteins (PAG) were determined in buffalo cows (Bubalus bubalis) using three different radioimmunoassay (RIA) systems (RIA-497, RIA-706, and RIA-708). Samples (10 mL) were collected by jugular venipuncture from Week 0 until Week 28 of pregnancy (9 females), and from parturition until Week 10 postpartum (13 females). During pregnancy, PAG molecules were detectable at Week 6 using the three aforementioned RIA systems (3.9 ± 1.3 ng/mL, 9.7 ± 1.3 ng/mL, and 9.9 ± 0.7 ng/mL for RIA-497, RIA-706, and RIA-708, respectively). These concentrations increased gradually until Week 28, reaching values of 39.6 ± 4.0 ng/mL (RIA-497), 50.5 ± 11.9 ng/mL (RIA-706), and 68.2 ± 20.8 ng/mL (RIA-708). PAG concentrations determined by RIA-497, RIA-706, and RIA-708 were strongly correlated throughout the entire gestation period, RIA-708 giving the higher concentrations. At parturition, the mean concentrations ranged from 34.9 ± 4.0 (RIA-497) to 84.7 ± 10.6 ng/mL (RIA-708). Thereafter, the concentrations decreased steadily, reaching very low levels (< 1.0 ng/mL) at Week 8 postpartum. In conclusion, PAG concentrations measured by the above three RIA systems showed a profile similar to those previously described in bovine species, with higher concentrations being detected by RIA-706 and RIA-708. However, the patterns of PAG concentrations, studied using the three aforementioned PAG-RIA systems, differed around parturition, with very low levels being observed in the female buffaloes [less ▲]

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See detailRecent possibilities for diagnosing early pregnancy (EP) in small ruminants and pigs.
Szenci, O; Beckers, Jean-François ULg; Taverne, M.A.M

in Reproduction in Domestic Animals (2008), 43(5), 104

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