Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses

in Plant Breeding (2010), 130

Gossypium australe F. Muell (2n + 2x = 26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n = 4x = 52), the main cultivated ... [more ▼]

Gossypium australe F. Muell (2n + 2x = 26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n = 4x = 52), the main cultivated cotton species. To isolate monosomic alien addition lines (MAALs) of G. australe in G. hirsutum, the [2(G. hirsutum X G. australe) X G. hirsutum] pentaploid (2n = 5x = 65) was backcrossed as male parent to G. hirsutum. Analysis of 42 BC1 plants and seven alien addition lines, already available, with 150 SSR markers developed from G. hirsutum revealed a cross-species amplification rate of 100% and a polymorphism rate of 56%. Eighty polymorphic SSR markers generated 87 G. australe-specific loci that have been assigned by a hierarchical cluster analysis to 13 linkage groups corresponding to the 13 chromosomes of G. australe. Analysis by SSR markers and genomic in situ hybridization of the self-progeny of disomic alien addition lines, backcross progeny of the pentaploid, allowed the isolation of five new MAALs. [less ▲]

Aspectos Generales del Cultivo in vitro

in Baudoin, Jean-Pierre; Aguirre, Gino (Eds.) Aplicación del Cultivo de Tejidos en la Multiplicación y conservación de los Recursos Fitogenéticos (2010)

Análisis comparativo del contenido de clorofila en 35 morfotipos de lisas (Ullucus tuberosus Caldas) en 3 Comunidades Atloandinas de Pisaq (Calca, Cusco)

in Primer Congreso Peruano de Mejoramiento Genético y Biotecnología Agricola (2010)

Principios sobre el mejoramiento genético de las leguminosas alimenticias del género Phaseolus

in Primer Congreso Peruano de Mejoramiento Genético y Biotecnología Agricola (2010)

Formación de compuestos raciales de maíz precoz (Zea mays L.) para comunidades altas de Cusco

in Primer Congreso Peruano de Mejoramiento Genético y Biotecnología Agricola (2010)

Conservación de germoplasma de tarwi (Lupinus mutabilis Sweet) en Cusco

in Primer Congreso Peruano de Mejoramiento Genético y Biotecnologia Agricola (2010)

Development of Upland Cotton Plants Highly Resistant to the Reniform Nematode From the G. Hirsutum x G. Thurberi x G. Longicalyx Hybrid

in Proceedings of the beltwide cotton improvement conference (2010)

Morphological and molecular diversity within Algerian cowpea (Vigna unguiculata (L.) Walp.) landraces

in Genetic Resources and Crop Evolution (2010), (57), 371-386

Determination of a Suitable Protocol for Indigenous Oilseed Cucurbits Plant Regeneration

in Tropicultura (2010), 28(4), 217-225

The present work was carried out to establish rapid in vitro propagation of the indigenous oilseed Citrullus lanatus. Efficiency of 3 protocols for seed decontamination was investigated. High level of ... [more ▼]

The present work was carried out to establish rapid in vitro propagation of the indigenous oilseed Citrullus lanatus. Efficiency of 3 protocols for seed decontamination was investigated. High level of seed sterilization was obtained after removing seed coat and soaking the seeds in 1.6 % sodium hypochlorite with a drop of Tween 20. Shoot tips, single node and cotyledon explants of 3 morphotypes have been screened for adventitious shoot formation in tissue culture. Best response in termis of multiple shoot induction was obtained from cotyledon proximal part with hypocotyl segmenet on Murashige and Skoog medium supplemented with 1 mg/l 6-Benzylaminopurine, 30 g/l sucrose and 8 g/l agar. After 3 weeks of culture, 90% of cotyledon proximal parts induced shoot. An average of 12.6 shoots per explants and a mean shoot length of 8 mm were obtained after multiplication stage. Shoot induction appeared to be strongly influenced by genotype and explant type. The percentage of shoot induction from cotyledon proximal parts ranged from 23.3% to 64.0% according to the genotype. Efficient rooting was achieved on half-solid MS medium containing 0.1 mg/l 1-Naphthaleneactic acid or without growth regulator. Acclimatised plantlets were transferred to greenhouse where they grew and flowered like seeded plantlets. [less ▲]

Use of ISSR markers to assess genetic diversity of African edible seeded Citrullus lanatus landraces

in Scientia Horticulturae (2010), 124