References of "Art, Tatiana"
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See detailExercise and epinephrine affect equine leukocytes distribution and gene expression in a distinct but closely related pattern
Ramery, Eve ULg; Schoenecker, Julie; Art, Tatiana ULg et al

in Veterinary Clinical Pathology (2011, December), 40(4), 583-584

Background: In horses-athletes, exercise has been shown to be an important regulator of immune cells and their functions, and catecholamines to be essential components of the physiological response to ... [more ▼]

Background: In horses-athletes, exercise has been shown to be an important regulator of immune cells and their functions, and catecholamines to be essential components of the physiological response to exercise. Objectives: The purpose of the present study was to compare immunological reactions and molecular events during exercise and epinephrine infusion. Methods: Horses were submitted to exercise or epinephrine infusion for 10 min. Blood samples were collected at T0, T5, Tl0, T20 and T40 min for biochemistry and haematology at T0 and T20 min for RNA extraction and microarray analysis. Results: In both protocols, packed-cell-volume and neutrophils peaked at T5 and T10, and returned to baseline at T40. Lymphocytes increased at T5 with exercise and at T20 with epinephrine and then remained high until the end of the protocols. Between T0 and T20, 55 probes, coding for 25 genes, and 19 probes, coding for 11 genes, were differentially regulated with epinephrine and exercise respectively (P<0.001). Ten of the genes over-expressed with epinephrine were related to inflammatory response and leukocytes trafficking and activation. Among the genes with the highest significant fold changes following exercise were FOS, DUSP-1 and CXCL1. FOS was over-expressed in both conditions. Among genes differentially regulated, Ingenuity software identified 3 overlapping functional networks with epinephrine and 1 functional network with exercise that overlapped the 3 epinephrine networks. Conclusions: Circulating leukocytes distribution and gene expression are rapidly affected following exercise and epinephrine infusion. Distinct but closely-related sets of gene activities were shown to be altered with exercise and epinephrine, with lesser changes in the immunological pattern with physical stress than epinephrine alone, arguing for compensatory mechanisms in exercise. [less ▲]

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See detailLe microdamier d’expression : un outil performant dans la compréhension de la réponse
Schoeneker, Julie; Art, Tatiana ULg; Pirottin, Dimitri ULg et al

in Annales de Médecine Vétérinaire (2011), 154

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans ... [more ▼]

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans l’amélioration de la santé et des résultats sportifs des athlètes. En effet, l’exercice peut être reconnu comme un stress. Il induit des modifications de l’équilibre homéostatique qui peuvent à leur tour altérer la réponse immunitaire de l’hôte et donc sa susceptibilité aux maladies. L’adrénaline est la molécule essentielle de tout processus de stress. La technologie des microdamiers, outil majeur d’investigation transcriptomique, permet l’étude de l’expression génique de l’ensemble du génome. Son utilisation devrait donc permettre de mieux caractériser et définir les interrelations entre l’exercice, le stress et la réponse immune. Cette revue recense les interrelations connues entre la réponse immune à un stress adrénergique d’une part et la réponse immune à l’exercice d’autre part. Elle considère en outre la contribution potentielle des microdamiers à une meilleure compréhension des effets d’un stress, et plus particulièrement celui lié à l’exercice, sur l’immunité. [less ▲]

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See detailInvestigation of the innate immunity in the lower respiratory tract in exercising horses
Frellstedt, Linda ULg; Gosset, Philippe; Desmet, Christophe ULg et al

in Proceedings of the 1st Scientific Meeting of the Faculty of Veterinary Medicine (2011)

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See detailApplied training physiology
Art, Tatiana ULg

in 5th Edition of the European Equine Health & Nutrition Congress (5th EEHNC) (2011)

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See detailInhalation with NDS27 attenuates pulmonary neutrophilic inflammation in recurrent airway obstruction
Sandersen, Charlotte ULg; Olejnik, Dorine; Franck, Thierry ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2011)

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See detailSubclinical diseases underlying poor performance in endurance horses: diagnostic methods and predictive tests.
Fraipont, Audrey ULg; Van Erck, E.; Ramery, Eve ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2011), 169(6), 154

Thirty-eight endurance horses underwent clinical and ancillary examinations, including haematological and biochemical evaluation, standardised exercise tests both on a treadmill and in the field, Doppler ... [more ▼]

Thirty-eight endurance horses underwent clinical and ancillary examinations, including haematological and biochemical evaluation, standardised exercise tests both on a treadmill and in the field, Doppler echocardiography, impulse oscillometry, video endoscopy and collection of respiratory fluids. All of the examined poorly performing horses were affected by subclinical diseases, and most of them had multiple concomitant disorders. On the contrary, the well-performing horses were free of any subclinical disease. The most frequently diagnosed diseases were respiratory disorders, followed by musculoskeletal and cardiac problems. Poor performers exhibited lower speeds at blood lactate concentration of 4 mmol/l (VLA4) and at heart rates of 160 (V160) and 200 bpm (V200) on the treadmill and in the field, as well as slower recovery of heart rate. [less ▲]

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See detailSubclinical diseases affecting performance in Standardbred trotters: Diagnostic methods and predictive parameters
Richard, Eric ULg; Fortier, Guillaume; Pitel, Pierre-Hugues et al

in Veterinary Journal (2010), 184(3), 282-289

The objectives of this study were to determine the prevalence of sub-clinical diseases in poorly-performing Standardbred horses, compare their physiological response to exercise with control horses, and ... [more ▼]

The objectives of this study were to determine the prevalence of sub-clinical diseases in poorly-performing Standardbred horses, compare their physiological response to exercise with control horses, and identify predictive parameters of poor-performance. Fifty horses underwent thorough clinical and ancillary examinations, including haematological and biochemical evaluation, Doppler echocardiography, standardised exercise tests (SETs) on both treadmill and racetrack, treadmill video-endoscopy and collection of respiratory fluids. Most of the poorly-performing horses exhibited many concomitant diseases. The most frequently diagnosed problems involved the lower and upper respiratory tract and the musculoskeletal system. Poor-performers had lower speeds at a blood lactate (LA) concentration of 4 mmol/L (VLA4) and a heart rate (HR) of 200 bpm (V200) on treadmill and racetrack, as well as lower values for haematological parameters, plasma angiotensin-converting enzyme and antioxidants, compared to control horses. Problems of the respiratory system were the most frequently diagnosed sub-clinical diseases affecting performance. SETs, together with some blood markers, may be useful as a non-specific diagnostic tool for early detection of diseases that may affect performance. [less ▲]

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See detailComparaison de l'endoscopie d'effort sur le terrain et sur tapis roulant chez le cheval de selle
Frippiat, Thibault ULg; Art, Tatiana ULg; Van Erck-Westergen, Emmanuelle

in Pratique Vétérinaire Equine (2010), 42(166), 23-29

Endoscopy of the upper airway in horses is done either at rest or during exercise, i. e. dynamic endoscopy. The present study compared observations made on 11 saddle horses botte with dynamic treadmill ... [more ▼]

Endoscopy of the upper airway in horses is done either at rest or during exercise, i. e. dynamic endoscopy. The present study compared observations made on 11 saddle horses botte with dynamic treadmill and dynamic field endoscopy.The results acquired with botte methods during strenuous exercise led to similar conclusions in terms of diagnosis and prognosis in 8 out of 11 cases. For the 3 others, the overground endoscopy revealed an abnormality that was net observable with treadmill endoscopy [less ▲]

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See detailPulmonary function, airway cytology and bronchoalveolar lavage fluid drug concentration after aerosol administration of cefquinome to horses
Art, Tatiana ULg; Ramery, Eve ULg; Fraipont, Audrey ULg et al

in Equine Veterinary Education (2010), 22(9), 473-479

The administration of antibiotics by aerosol to horses suffering from respiratory infections may partially circumvent the limitations of antimicrobial therapy, e.g. large injection volumes, low ... [more ▼]

The administration of antibiotics by aerosol to horses suffering from respiratory infections may partially circumvent the limitations of antimicrobial therapy, e.g. large injection volumes, low bioavailability and risk of diarrhea. Only injectable formulations are available currently and usually contain other substances that could irritate the mucosa and induce coughing and bronchospasm. In addition, the quality of the aerosol, particularly in terms of the delivery of antibiotics to the deep parts of the lung, is unknown. Although used under field conditions, cefquinome delivered by aerosol has never been studied in horses. This study examined the safety of cefquinome injectable solution, administered by aerosol at a dose of 225 mg/inhalation to 7 healthy horses, by assessing (1) pulmonary function before and 15 min after a single inhalation, at the first day (Day 1) and the fifth day (Day 5) of a 5 day period treatment; and (2) the inflammatory status of the lung, i.e. percentage neutrophils and myeloperoxidase concentration, based on bronchoalveolar lavage (BAL) at D1 and D5. In addition, cefquinome concentrations were measured in bronchoalveolar lavage fluid after aerosol, intravenous (i.v.) and intramuscular (i.m.) administrations. A single aerosol of cefquinome injectable solution did not induce any immediate nor delayed pulmonary side effects in healthy horses and produced cefquinome concentrations in bronchoalveolar lavage (BAL) within 30 min that were higher than the minimal inhibitory concentration of the main equine respiratory pathogens. These results should stimulate further studies, especially in horses suffering from bronchial hyper-reactivity. Aerosol delivery of antibiotics may well have a role in equine therapeutics. [less ▲]

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See detailEndoscopie à l'effort : les examens sur piste et sur tapis roulant sont-ils équivalents ?
Van Erck, Emmanuelle ULg; Frippiat, Thibault ULg; Dupuis, Marie-Capucine ULg et al

in Proceedings des Journées annuelles de l’Association vétérinaire équine française (2009) (2009, October)

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See detailInfluence of subclinical inflammatory airway disease on equine respiratory function evalueated by impulse oscillometry
Richard, Eric ULg; Fortier, Guillaume; Denoix, Jean-Marie et al

in Equine Veterinary Journal (2009), 41(4), 384-389

Reasons for performing study: Inflammatory airway disease (IAD) is a nonseptic condition of the lower respiratory tract. Its negative impact on respiratory function has previously <br /><br />been ... [more ▼]

Reasons for performing study: Inflammatory airway disease (IAD) is a nonseptic condition of the lower respiratory tract. Its negative impact on respiratory function has previously <br /><br />been described using either forced expiration or forced oscillations techniques. However, sedation or drug-induced bronchoconstriction were usually required. The impulse <br /><br />oscillometry system (IOS) is a noninvasive and sensitive respiratory function test validated in horses, which could be useful to evaluate IAD-affected horses without further <br /><br />procedures. <br /><br />Objectives: To determine the sensitivity of IOS in detecting alterations of the respiratory function in subclinically IAD-affected horses without inducing bronchoprovocation and to characterise their respiratory impedance according to frequency for each respiratory phase. <br /><br />Methods: Pulmonary function was evaluated at rest by IOS in 34 Standardbred trotters. According to the cytology of bronchoalveolar lavage fluid (BALF), 19 horses were defined <br /><br />as IAD-affected and 15 horses were used as control (CTL). Total respiratory resistance (Rrs) and reactance (Xrs) from 1–20 Hz as well as their inspiratory and expiratory <br /><br />components were compared between groups. <br /><br />Results: A significant increase of Rrs at the lower frequencies (R1–10 Hz) as well as a significant decrease of Xrs beyond 5 Hz (X5–20 Hz) was observed in IAD compared to CTL horses. IOS-data was also significantly different between inspiration and expiration in IAD-affected horses. In the whole population, both BALF eosinophil and mast cell counts were <br /><br />significantly correlated with IOS measurements. <br /><br />Conclusions: Functional respiratory impairment may be measured, even in the absence of clinical signs of disease. In IAD-affected horses, the different parameters of respiratory <br /><br />function (Rrs or Xrs) may vary depending on the inflammatory cell profiles represented in BALF. <br /><br />Potential relevance: Impulse oscillometry could be used in a routine clinical setting as a noninvasive method for early detection of subclinical respiratory disease and of the results <br /><br />of treatment in horses. [less ▲]

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See detailL'analyse des gaz sanguins artériels dans l'examen du cheval contre-performant
Art, Tatiana ULg; Van Erck, Emmanuelle ULg

in Pratique Vétérinaire Equine (2009), 41(162), 57-64

This paper analyses the usefulness of arterial blood gas analysis (ABG) as ancillary test during the examination of the poor performance horse. The principle of the measurement of the gas tensions and the ... [more ▼]

This paper analyses the usefulness of arterial blood gas analysis (ABG) as ancillary test during the examination of the poor performance horse. The principle of the measurement of the gas tensions and the methodological rules to be respected are presented. The functional troubles inducing impairments of pulmonary gas exchanges are described. Several papers reporting the effect of upper and lower airway dysfunction on ABG have been published. On the basis on this scientific information, the usefulness and the limits of the ABG analyses as ancillary test in the examination of the poor performer are presented and discussed [less ▲]

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See detailIntérêt du suivi sportif à la piste des chevaux d’endurance dans la détection des problèmes subcliniques
Fraipont, Audrey ULg; Van Erck, Emmanuelle; Art, Tatiana ULg et al

in 35ème Journée de la Recherche équine (2009)

The aim of this study was to evaluate a field exercise test specifically designed for endurance horses and to determine the relevant parameters allowing discrimination between healthy horses (H) and ... [more ▼]

The aim of this study was to evaluate a field exercise test specifically designed for endurance horses and to determine the relevant parameters allowing discrimination between healthy horses (H) and horses presenting subclinical disorders (SP). Twenty four endurance horses underwent blood analyses, general examination and special exams to define a posteriori 2 groups: group H composed of healthy horses (n=12) and group SP included horses presenting subclinical disorders (n=12). The exercise test consisted of a warm-up followed by 3 gallop steps (27 km, 1.5km, 1.5km) at incremental speed. Lactates were measured after each step, an ECG was recorded during effort and recovery, speed was monitored by a GPS. Significant differences were observed between H and SP groups for VLA4, V160 and heart rate during recovery with better values for H horses. These results demonstrate the pertinence of a medical athletic follow-up in endurance horses: this field exercise test is easily achievable and allows the detection of subclinical disorders which would otherwise remain unsuspected by the riders. [less ▲]

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See detailLe microdamier: un outil du futur pour le diagnostic de l’inflammation ?
Mignot, Clémence ULg; Fraipont, Audrey ULg; Richard, Eric et al

Poster (2009)

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See detailUpper airway dynamic endoscopy: are track and treadmill observations comparable ?
Van Erck, Emmanuelle ULg; Frippiat, Thibault ULg; Dupuis, Marie-Capucine ULg et al

in Tessier, Caroline; Gerber, Vincent (Eds.) Abstract book: 4th World Equine Airways Symposium (WEAS) (2009)

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See detailRespiratory inflammation in endurance horses
Van Erck, Emmanuelle ULg; Art, Tatiana ULg

in Tessier, Caroline; Gerber, Vincent (Eds.) Abstract book: 4th World Equine Airways Symposium (WEAS) (2009)

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