Contribution à l'étude de la pathogénie de l'infection par Trypanosoma evansi

Doctoral thesis (2009)

Flow cytometric enumeration of parasitaemia and haematologic changes in trypanosoma-infected mice.

in Acta Tropica (2008), 107(2), 139-44

African trypanosomiasis is a severe parasitic disease affecting both man and livestock. It is crucial to expand our fundamental knowledge of the intimate interactions between trypanosomes and their ... [more ▼]

African trypanosomiasis is a severe parasitic disease affecting both man and livestock. It is crucial to expand our fundamental knowledge of the intimate interactions between trypanosomes and their vertebrate hosts in order to develop new and efficient control strategies. The mouse model of trypanosomiasis is the most popular for research purposes because of all the logistic advantages of using this species. Studies of any aspect of trypanosomiases in the mouse systematically require the quantification of some phenotypic traits which translate its degree of resistance/susceptibility to the disease, as blood cell counts. The present study presents a methodological approach combining everyday microsampling of tail blood and its analysis by flow cytometry. The technical options and conditions permitting a fast, reliable and reproducible daily quantification of erythrocyte, reticulocyte, leucocyte and trypanosome counts in the inoculated mouse were established. The protocol proposed allows the multiplication of blood samplings without being exposed to the time-consuming constraint of visual countings, without causing iatrogenic blood cell alterations in the mouse and without requiring specific anti-trypanosome antibodies. [less ▲]

Pathologie de l'infection par Trypanosoma evansi

in Annales de Médecine Vétérinaire (2008), 152(2), 94-102

Trypanosoma evansi is an extracellular parasite found in the blood and tissues of mammals, mainly causing anaemia, immune depression and central nervous system disorders. Contrary to other African ... [more ▼]

Trypanosoma evansi is an extracellular parasite found in the blood and tissues of mammals, mainly causing anaemia, immune depression and central nervous system disorders. Contrary to other African trypanosomes, T. evansi is mechanically transmitted and thus presents a worldwide distribution. This review aims at summarizing clinical and pathological data about T. evansi infections in its various hosts. It presents the actual knowledge on pathogenesis and the recent development of which open new ways for infection control. [less ▲]

Daily haematologic follow-up of trypanosomiasis among inbred mouse strains reveals a phenotypic variation wider than commonly assumed

Poster (2007, October)

Tuareg ethnoveterinary treatments of camel diseases in Agadez area (Niger)

in Tropical Animal Health and Production (2007), 39(2), 83-89

For generations, nomadic herders have been learning to manage herd health, particularly in dromedaries because of their great value. Owing to the unavailability of veterinary services, camel herders in ... [more ▼]

For generations, nomadic herders have been learning to manage herd health, particularly in dromedaries because of their great value. Owing to the unavailability of veterinary services, camel herders in remote areas have been developing their own pharmacopoeia and veterinary techniques. The bleeding of sick animals is a common treatment, as Tuareg herders believe that 'tainted blood' (izni) is the cause of many conditions. Several surgical techniques are also used, such as excision of calcified sublingual cord. The remedies mentioned in this survey are derived from Maerua crassifolia, Boscia senegalensis, Acacia raddiana, Cucumis prophetarum, Calotropis procera, Ricinus communis, Citrullus colocynthis, green tea, millet, tobacco and onions. Artificial elements are also used for treatment of animals: Powders collected from batteries, various haircare or skincare creams, crushed glass, insecticides or motor oil belong to their pharmacopoeia. This broadmindedness allows the introduction of modern veterinary medicine. Factors such as the lack of real production objectives constitute limits to this progress, however. [less ▲]

Trypanosoma evansi infection in a murine model: new insights for study of camel trypanosomiasis

Conference (2006, April)

Flow Cytometry for the follow-up of Trypanosoma evansi infection in a mouse model

Poster (2005, November 18)

Enquête sur les pratiques ethno-vétérinaires et dominantes pathologiques dans les élevages camelins Touaregs à Agadez, Niger

Master's dissertation (2004)