References of "Antoine, Nadine"
     in
Bookmark and Share    
Full Text
See detailThe three-dimensional reconstruction of the innervation pattern in the lymphoid compartment of the ovine pharyngeal tonsil highlighted a possible way of neuro-invasion by the scrapie agent.
Toppets, Vinciane ULg; Piret, Joëlle ULg; Grobet, Luc ULg et al

in Proceedings of the 2nd Scientific Meeting of the Faculty of Veterinary Medicine, ULg, Belgium, October 19, 2012 (2012, October 19)

Detailed reference viewed: 16 (2 ULg)
Full Text
Peer Reviewed
See detailMorphological and morphometric analyses of the suspensory ligament in Standardbreds
Shikh Al Sook, Mohamad Khir ULg; Antoine, Nadine ULg; Piret, Joëlle ULg et al

Poster (2012, October)

Ultrasound techniques allow examination of some parts of the suspensory ligament (SL) but "anomalies" are regularly observed. Their significance is not known. Few studies have described the relationship ... [more ▼]

Ultrasound techniques allow examination of some parts of the suspensory ligament (SL) but "anomalies" are regularly observed. Their significance is not known. Few studies have described the relationship between ultrasonographic appearance and the exact morphology in histological sections. The aim of this study is to develop good techniques for cutting, staining, and showing the variation in the tissue composition within the SL. The SLs from the right limbs of 11 horses were collected. Samples were taken from cross-sections at six levels of the SL and they were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. By using the cryosection technique, some freezing artifacts (holes) appeared. Therefore, a technique of freezing with cryoprotection was carried out, which produced the best results. Hematoxylin-phloxine-saffron gives a good contrast of colors between the tissues observed allowing the use of an image analysis program. The percentage of each tissue within the SL for each section and for six levels of the ligament was calculated. Results were analyzed by SAS software. The muscle tissue (PMT) and adipose tissue (PAT) decreased significantly (p < 0.0001), whereas the connective tissue (PCT) increased significantly (p < 0.0001) with age and when descending from the proximal to the distal level of the SL. The PMT was significantly higher (p < 0.0001) in females than males, while the PCT was significantly higher (p < 0.0001) in males than females. The PAT was significantly higher (p = 0.0278) in hindlimbs than in forelimbs. [less ▲]

Detailed reference viewed: 595 (434 ULg)
Full Text
Peer Reviewed
See detailNeuroimmune connections in ovine pharyngeal tonsil: potential site for prion neuroinvasion
Toppets, Vinciane ULg; Piret, Joëlle ULg; Kirschvink, Nathalie et al

in Cell & Tissue Research (2012)

Recent studies have proved the possible implication of nasal associated lymphoid tissues, mainly the pharyngeal tonsil, in prion pathogenesis. However, the mechanisms of this neuroinvasion are still being ... [more ▼]

Recent studies have proved the possible implication of nasal associated lymphoid tissues, mainly the pharyngeal tonsil, in prion pathogenesis. However, the mechanisms of this neuroinvasion are still being debated. To determine the potential sites for prion neuroinvasion inside the ovine pharyngeal tonsil, the topography of neurofilaments heavy (200 kDa) (NFH), neurofilaments light (70 kDa) (NFL) and glial fibrillar acidic protein (GFAP) was semi-quantitatively analysed inside the different compartments of the tonsil. The results showed that the most innervated areas were the interfollicular area and the connective tissue located beneath the respiratory epithelium. Even if the germinal centre of the lymphoid follicles was poorly innervated, the existence of rare follicular dendritic cell-nerve synapses inside the germinal centre indicates that this mechanism of neuroinvasion is possible but unlikely to be unique. The host PRNP genotype did not influence the pattern of innervation in these different tonsil compartments, unlike age: an increase of nerve endings in a zone of high trafficking cells beneath the respiratory epithelium occurred with ageing. A minimal age-related increase of innervation inside the lymphoid follicles was also observed. An increase in nerve fibre density around the lymphoid follicles, in an area rich in mobile cells able to transport PrPd, could ensure a more efficient infectivity, not in the early phase but in the advanced phase of lymphoinvasion after amplification of PrPd, or could act as direct site of entry during neuroinvasion. [less ▲]

Detailed reference viewed: 16 (4 ULg)
Full Text
Peer Reviewed
See detailSubtilisin Sub3 is involved in adherence of Microsporum canis to human and animal epidermis
Bagut, ET; Baldo, A; Mathy, Anne ULg et al

in Veterinary Microbiology (2012), 160(3-4), 413-419

Detailed reference viewed: 37 (12 ULg)
Full Text
Peer Reviewed
See detailPrevalence of naturally occuring cartilage defects in the ovine stifle
Pierson, Audrey ULg; Muylkens, Benoît ULg; Hontoir, Fanny et al

Poster (2012)

Detailed reference viewed: 27 (9 ULg)
Full Text
Peer Reviewed
See detailMechanisms of skin adherence and invasion by dermatophytes
Baldo, Aline ULg; Monod, M.; Mathy, Anne ULg et al

in Mycoses (2012), 55(3), 218-223

Detailed reference viewed: 34 (12 ULg)
Full Text
Peer Reviewed
See detailMorphological and histological studies of sheep’s brain
Salouci, Moustafa ULg; Engelen, Virginie; Jacqmot, Olivier ULg et al

Poster (2011, December)

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the ... [more ▼]

Introduction: The study of normal structures of the sheep’s brain is very important to understand pathological changes caused by the bluetongue virus in the fetus’s brain at various stages of the gestation. Bluetongue is an arthropod-borne viral disease of domestic and wild ruminants. The serotype 8 is responsible for outbreaks in Northern Europe in 2006. This virus causes lesions in the brain of fetuses as hydrancephaly and porencephaly. The aim of this work is to improve knowledge of anatomy and histology of the central nervous system of the sheep. Methods: Seven heads of adult sheep and one from a fetus aged 4,5 months were used. All heads were first opened in the frontal area using bone’s saw and immerged in a formalin solution for 10 days. After a good fixation, the brains were extracted and sectioned. Transversal, frontal and sagittal sections were realized. The sections of two brains were stained with Berlin-blue and treated to be embedded in methylmetacrylate for gross morphology. The different parts of the 6 resting brains were then embedded in paraffin, cut and the histological sections were stained with haematoxylin/eosin, cresyl violet or by use of silver impregnation. Results: Gross morphological examination of the brains embedded in methylmetacrylate showed the detailed anatomy of the different parts. The staining with haematoxylin/eosin permitted to differentiate the grey matter, the different nucleus and the layers of cerebral and cerebellum cortex. The cresyl violet technique permitted to visualize the Nissl bodies and the silver impregnation revealed nerve fibers. In the fetus brain, blood vessels were very numerous in the brainstem, the cerebellum and the cerebrum. The grey matter was less organized and looser. Conclusion: This work establishes an anatomical and histological approach allowing future studies in ovine fetuses with and without brain lesions potentially caused by the bluetongue virus. [less ▲]

Detailed reference viewed: 41 (12 ULg)
Full Text
Peer Reviewed
See detailFeatures of follicular dendritic cells in ovine pharyngeal tonsil: An in vivo and in vitro study in the context of scrapie pathogenesis
Toppets, Vinciane ULg; Defaweux, Valérie ULg; Piret, Joëlle ULg et al

in Veterinary Immunology and Immunopathology (2011), 141

Although the alimentary tract has been suggested as the most likely portal of entry in natural scrapie, a growing amount of data indicates that the respiratory system and more specifically the pharyngeal ... [more ▼]

Although the alimentary tract has been suggested as the most likely portal of entry in natural scrapie, a growing amount of data indicates that the respiratory system and more specifically the pharyngeal tonsils serve as a natural portal of entry for scrapie. This study describes for the first time the broad cell populations in the lymphoid compartment of pharyngeal tonsils and more specifically inside the lymphoid follicles where the scrapie agent accumulates during the period of latency. Follicular dendritic cells (FDCs), stromal cells located in the light zone of the germinal centre of lymphoid follicles, seem to be the principal causal factor in the accumulation of the infectious agent in transmissible spongiform encephalopathy (TSE) diseases. Knowing that efficient lymphoreticular prion propagation requires PrPc expression, we analysed the expression of PrPc with the mouse monoclonal antibody Pri 909 both in situ and on FDC-cluster-enriched cell suspensions. In situ, a positive staining was observed in the germinal centre of pharyngeal lymph follicles. The germinal centre labelling was due to the presence of a follicular dendritic network as revealed after immunogold staining of isolated FDC clusters. Our results suggest that the pharyngeal lymphoreticular system and more specifically PrPc expressing follicular dendritic cells could serve as a prion “reservoir” during the latency phase, thus playing a key role during the scrapie lymphoinvasion. [less ▲]

Detailed reference viewed: 18 (4 ULg)
Full Text
Peer Reviewed
See detailMorphology of the suspensory ligament (interosseous muscle III) of the horse
Shikh Al Sook, Mohamad Khir ULg; Espinosa, Jennifer; Piret, Joëlle ULg et al

Poster (2011)

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL ... [more ▼]

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL. The significance of “abnormal” findings is however not sufficiently known. Until now, few studies described the relationship between the ultrasonographic appearance and the exact morphology in histological sections. The aim of this study is to develop good techniques for cutting and staining the SL and to improve knowledge about the normal morphology of the SL. Methods: In this study, the SL of eight <sound> horses were collected. The body of the SL was divided in 3 thirds and sampling was realised within each third and between the thirds. The samples were embedded in paraffin or in Tissue-Tek for cryosections. The sections were stained with hematoxylin/eosin or Masson's trichrome. For 3 SL, ultrasounds were performed before sampling. The digital tip was maintained in physiological position owing to a press. Results: Most of the paraffin sections were shredded because of the hardness of the tissue. Cryosection revealed a better preservation of tissues. Only some freezing artifacts (holes) appeared on a few sections. Muscles fibers surrounded by adipose tissue containing blood vessels were present mainly in the proximal and medium third of the SL whereas they were not found in the distal third. The remaining structure look like a tendon and was composed of collagen fibers, stained in green with the Masson's trichrome coloration. Conclusions: This study permitted to develop cutting and staining techniques for the SL and helped to map the adipose, muscular and tendinous parts within the SL. It lays down the bases of subsequent studies that will concern ultrasonographically examined digital tips of sound and pathological horses of different breeds and ages. [less ▲]

Detailed reference viewed: 296 (121 ULg)
Full Text
Peer Reviewed
See detailRôle des cellules Th17 dans les maladies infectieuses et auto-immunes
Cambier, Ludivine ULg; Defaweux, Valérie ULg; Baldo, Aline ULg et al

in Annales de Médecine Vétérinaire (2011), 154

Detailed reference viewed: 133 (35 ULg)
Full Text
Peer Reviewed
See detailEffects of formoterol and ipratropium bromide on repeated cadmium inhalation -induced pulmonary inflammation and emphysema in rats
Zhang, Whenhui; Fievez, Laurence ULg; Zhang, F. et al

in European Journal of Pharmacology (2010), 25(647), 178-187

Detailed reference viewed: 53 (9 ULg)
Full Text
Peer Reviewed
See detailMSH2 gene dosage mediates azathioprine-induced carcinogenesis in mice
Chalastanis, A.; Penard-Lacronique, V.; Svrcek, M. et al

in Journal of the National Cancer Institute (2010)

Detailed reference viewed: 60 (33 ULg)
Full Text
Peer Reviewed
See detailMorphology of the digital sheath in horses
Strzalkowski, A; Espinosa, J; Jacqmot, O et al

in Anatomia, Histologia, Embryologia (2010, August), 39(4), 288

Introduction: The digital sheath is located in the palmar (plantar) face of the distal limb. It consists of a synovium, divided into intimate and vascularised supportive layers, and ligaments that ... [more ▼]

Introduction: The digital sheath is located in the palmar (plantar) face of the distal limb. It consists of a synovium, divided into intimate and vascularised supportive layers, and ligaments that strengthen the tendon sheath in its palmar (plantar) face. The synovium surrounds the digital flexor tendons during their passage within the sheath. The synovium fluid, produced by the filtering of the blood and by the intima cells, allows lubrification within the sheath. Lameness, originating from digital sheath pathology is not rare, and it is important, for any equine practitioner, to know its normal morphology. The aim of this study is to precise the morphology of the digital sheath, particularly its synovium because the literature is not unanimous. Methods: The digitals tips of 7 "sound" horses were collected. Samples were taken within the 3 annular ligaments (Proximal, Digital Proximal, Digital Distal). The digital flexor tendons were sampled with the visceral sheath of the synovial membrane at the level of the proximal sesamoïd bones and just proximal to the medium scutum. All these samples were embedded in tissue-tek and freezed for cryosectioning. The proximal and distal recessus of the synovial membrane were also sampled, fixed in formalin and embedded in paraffin. Sections were stained with haematoxylin/eosin. One sample of the synovial membrane was taken for electronic microscopy and embedded in? Results: Annular ligaments are composed of dense connective tissue but are transversal strengthening of the fascia rather than true ligaments. The digital flexor tendons showed the typical organisation of the collagen network. Type III fibrocytes were observed within the deep digital flexor tendon. The synovial membrane showed two layers: an intima (with visceral and parietal sheath) with fibroblast-like cells and macrophagic cells, and a sub-intima composed of fibrous tissue that was sometime so thickened that it was difficult to cut! The recessus contained large synovial fringe with adipose tissue. Conclusion: This study permitted to precise the morphology of the digital sheath in horse which may help to better understand the pathological changes. [less ▲]

Detailed reference viewed: 30 (3 ULg)
Peer Reviewed
See detailComparison of MRI images obtained at 7T in a dog to macroscopic and histopathological examination
Van Thielen, B.; Visser, F.; Denolin, V. et al

Poster (2010, July 20)

Detailed reference viewed: 18 (4 ULg)
Full Text
Peer Reviewed
See detailLes prions exploitent les communications neuro-immunitaires
Dorban, Gauthier; Antoine, Nadine ULg; Defaweux, Valérie ULg

in Medecine Sciences : M/S (2010), 26(6),

Detailed reference viewed: 18 (9 ULg)