References of "Angenot, Luc"
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See detailIn vitro and in vivo antimalarial and cytotoxic activity of five plants used in Congolese traditional medicine.
Lusakibanza, M.; Mesia, G.; Tona, G. et al

in Journal of Ethnopharmacology (2010), 129

AIM OF THE STUDY: The in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ... [more ▼]

AIM OF THE STUDY: The in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ethnobotanical survey conducted in D.R. Congo and focusing on plants used traditionally to treat malaria. The in vivo antimalarial activity of aqueous and methanolic extracts active in vitro was also determined in mice infected by Plasmodium berghei berghei. MATERIALS AND METHODS: The growth inhibition of Plasmodium falciparum strains was evaluated using the measurement of lactate dehydrogenase activity. The extracts (aqueous, CH(3)OH, EtOH and CH(2)Cl(2)) were prepared by maceration and tested in vitro against the 3D7 (chloroquine sensitive) and W2 (chloroquine resistant) strains of Plasmodium falciparum and against the human normal fetal lung fibroblasts WI-38 to determine the selectivity index. Some extracts were also used at the dose of 300mg/kg to evaluate their activity in mice infected since 4 days by Plasmodium berghei. RESULTS: Two plants presented a very high activity (IC(50)<3mug/ml). These plants were Strychnos icaja roots bark (MeOH and CH(2)Cl(2)) and Physalis angulata leaves (MeOH and CH(2)Cl(2)). One plant (Anisopappus chinensis whole plant, MeOH and CH(2)Cl(2)) presented a high activity (IC50<15mug/ml). The extracts of Anisopappus chinensis and Physalis angulata showed also a good inhibition of parasitemia in vivo. Flavonoids, phenolic acids and terpenes were identified in these plants by a general phytochemical screening method. CONCLUSION: Three plants showed a very interesting antiplasmodial activity (Anisopappus chinensis, Physalis angulata and Strychnos icaja) and one of them showed a good selectivity index (>10, Anisopappus chinensis). Anisopappus chinensis and Physalis angulata were also active in vivo. [less ▲]

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See detailEvaluation des potentialités antiplasmodiales de plantes utilisées en médecine traditionnelle au Burkina Faso
Jansen, Olivia ULg; Nikiéma, Jean-Baptiste; Angenot, Luc ULg et al

Conference (2009, October 07)

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See detailPlantes médicinales et paludisme
Angenot, Luc ULg

Conference (2009, October 07)

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See detailPharmacognosie Volume 1
Angenot, Luc ULg; Tits, Monique ULg

Learning material (2009)

Le volume 1 est principalement consacré à la phytochimie. Le cours est subdivisé suivant les différentes classes chimiques des métabolites primaires et secondaires impliqués soit dans l'activité des ... [more ▼]

Le volume 1 est principalement consacré à la phytochimie. Le cours est subdivisé suivant les différentes classes chimiques des métabolites primaires et secondaires impliqués soit dans l'activité des plantes soit dans leur toxicité ou encore présentant des applications en pharmacotechnie ou dans l'alimentation. La connaissance des structures ( basée sur leur origine biosynthétique) est très importante pour évaluer leur solubilité, leur stabilité et ainsi mieux comprendre les processus d'extraction et les méthodes spécifiques de contrôle ( plantes entières ou pulvérisées, , extraits, huiles essentielles, huiles grasses, exsudats...). dans de nombreux cas, une relation structure-activité pharmacologique ou physiologique est établie. [less ▲]

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See detailAntisickling properties of divanilloylquinic acids isolated from Fagara zanthoxyloides Lam. (Rutaceae).
Ouattara, Badiore; Jansen, Olivia ULg; Angenot, Luc ULg et al

in Phytomedicine : International Journal of Phytotherapy and Phytopharmacology (2009), 16(2-3), 125-129

Fagara zanthoxyloides Lam. (syn. Zanthoxylum zanthoxyloides) (Rutaceae) is the most cited Fagara species for the treatment and the prevention of sickle cell disease crisis. Sickle cell anemia (SCA) is a ... [more ▼]

Fagara zanthoxyloides Lam. (syn. Zanthoxylum zanthoxyloides) (Rutaceae) is the most cited Fagara species for the treatment and the prevention of sickle cell disease crisis. Sickle cell anemia (SCA) is a public health problem in many countries particularly in Africa. The present study was designed to evaluate the antisickling properties of three isomeric divanilloylquinic acids (3,4-O-divanilloylquinic acid or burkinabin A; 3,5-O-divanilloylquinic acid or burkinabin B and 4,5-O-divanilloylquinic acid or burkinabin C) identified previously by LC/MS/NMR analysis in the root bark of F. zanthoxyloides [Ouattara et al., 2004. LC/MS/NMR analysis of isomeric divanilloylquinic acids from the root bark of Fagara zanthoxyloides Lam. Phytochemistry 65, 1145-1151]. The three isomers showed interesting antisickling properties which increased from burkinabins A to C. [less ▲]

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See detailThiaminylated adenine nucleotides — chemical synthesis, structural characterization and natural occurrence
Frederich, Michel ULg; Delvaux, David ULg; Gigliobianco, Tiziana ULg et al

in FEBS Journal (2009), 276(12), 32563268

Thiamine and its three phosphorylated derivatives (mono-, di- and triphosphate) occur naturally in most cells. Recently, we reported the presence of a fourth thiamine derivative, adenosine thiamine ... [more ▼]

Thiamine and its three phosphorylated derivatives (mono-, di- and triphosphate) occur naturally in most cells. Recently, we reported the presence of a fourth thiamine derivative, adenosine thiamine triphosphate (AThTP), produced in E. coli in response to carbon starvation. Here, we show that the chemical synthesis of AThTP leads to another new compound, adenosine thiamine diphosphate (thiaminylated ADP, AThDP), as a side product. The structure of both compounds was confirmed by mass spectrometry and 1H-, 13C- and 31P-NMR and some of their chemical properties were determined. Our results show an upfield shifting of the C-2 proton of the thiazolium ring in adenosine thiamine derivatives compared to the conventional thiamine phosphate derivatives. This modification of the electronic environment of the C-2 proton might be explained by a through-space interaction with the adenosine moiety, suggesting an U-shaped folding of adenosine thiamine derivatives. Such a structure where the C-2 proton is embedded in a closed conformation can be located using molecular modeling as an energy minimum. In E. coli, AThTP may account for 15% of total thiamine under energy stress. It is less abundant in eukaryotic organisms, but is consistently found in mammalian tissues and in some cell lines. Using a HPLC method, we show for the first time that AThDP may also occur in small amounts in E. coli and in vertebrate liver. The discovery of two natural thiamine adenine compounds further highlights the complexity and diversity of thiamine biochemistry, which is not restricted to the cofactor role of thiamine diphosphate. [less ▲]

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See detailIn Vitro Anticancer Potential of Tree Extracts from the Walloon Region Forest.
Frederich, Michel ULg; Marcowycz, Aline; Cieckiewicz, Ewa ULg et al

in Planta Medica (2009), 75(15), 1634-1637

Forty-eight extracts from 16 common Belgian trees from the Walloon Region forest were evaluated for IN VITRO growth inhibitory activity against the human LoVo colon cancer, PC3 prostate cancer, and U373 ... [more ▼]

Forty-eight extracts from 16 common Belgian trees from the Walloon Region forest were evaluated for IN VITRO growth inhibitory activity against the human LoVo colon cancer, PC3 prostate cancer, and U373 glioblastoma cell lines. Our study was performed with the aim of selecting plant candidates in order to later isolate new anticancer compounds from an easily affordable tree material. Extracts from ALNUS GLUTINOSA (stem bark), CARPINUS BETULUS (leaves and stem bark), CASTANEA SATIVA (stem bark), FAGUS SYLVATICA (leaves), ILEX AQUIFOLIUM (leaves), LARIX DECIDUA (leaves), QUERCUS PETRAEA (stem bark), and QUERCUS ROBUR (leaves) showed for the first time potent IN VITRO growth inhibitory activity and could become easily affordable sources of potential new anticancer agents. Root extracts from ROBINIA PSEUDOACACIA, already known for containing cytotoxic lectins, also showed interesting activity. [less ▲]

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See detailFagraldehyde, a Secoiridoid Isolated from Fagraea fragrans
Jonville, Marie ULg; Capel, Marie; Frederich, Michel ULg et al

in Journal of Natural Products (2008), 71(12), 2038-2040

A secoiridoid aglycone with atypical skeleton, named fagraldehyde (1), together with several known secoiridoids (gentiopicroside (2), sweroside (3) and swertiamarin (4)) were isolated from the bark and ... [more ▼]

A secoiridoid aglycone with atypical skeleton, named fagraldehyde (1), together with several known secoiridoids (gentiopicroside (2), sweroside (3) and swertiamarin (4)) were isolated from the bark and leaves of Fagraea fragrans collected in Cambodia. The conformations of 1 were evaluated on the basis of molecular modeling and NOESY correlations. A hypothetical biogenesis of fagraldehyde was proposed to explain the unusual skeleton. Compound 1 was weakly active in vitro against Plasmodium falciparum. [less ▲]

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See detailA la découverte de médicaments d'origine végétale- la Pharmacie Verte
Angenot, Luc ULg

Article for general public (2008)

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See detailEthnopharmacologie et paludisme au Burkina Faso : sélection de 13 espèces à potentialités antiplasmodiales méconnues
Jansen, Olivia ULg; Frederich, Michel ULg; Tits, Monique ULg et al

in Ethnopharmacologia (2008), 41

Dans la recherche de nouvelles substances actives contre les Plasmodium, les flores non explorées du Sud constituent une source potentielle privilégiée de nouveaux médicaments antipaludéens. Dans ce ... [more ▼]

Dans la recherche de nouvelles substances actives contre les Plasmodium, les flores non explorées du Sud constituent une source potentielle privilégiée de nouveaux médicaments antipaludéens. Dans ce travail, nous avons suivi une démarche ethnopharmacologique afin de répertorier et de sélectionner des végétaux intéressants à étudier en laboratoire pour leurs propriétés antiplasmodiales. Notre travail de recensement des espèces utilisées contre la malaria au Burkina Faso nous a permis de répertorier 72 espèces végétales utilisées seules ou en association dans le traitement traditionnel du paludisme dans ce pays d’Afrique de l’Ouest. Finalement, treize espèces ont été sélectionnées et dix-sept échantillons végétaux ont été récoltés au Burkina Faso pour évaluation de leurs propriétés antiplasmodiales en laboratoire. Les principaux critères de sélection ont été : leur utilisation traditionnelle contre la malaria et le fait que ces plantes n’aient pas (ou peu) été étudiées sur le plan antiplasmodial. Les liens de chimiotaxonomie éventuels avec des plantes déjà connues pour leurs propriétés antiplasmodiales ainsi que les possibilités de valorisation des espèces au niveau local (MTA) ont également été considérés. [less ▲]

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See detailLes origines du métier de pharmacien
Angenot, Luc ULg

Article for general public (2008)

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See detailPotential antimalarial activity of indole alkaloids
Frederich, Michel ULg; Tits, Monique ULg; Angenot, Luc ULg

in Transactions of the Royal Society of Tropical Medicine & Hygiene (2008), 102(1), 11-19

New antimalarial treatments are now urgently required, following the emergence of resistance to the most used drugs. Natural products contribute greatly to the therapeutic arsenal in this area, including ... [more ▼]

New antimalarial treatments are now urgently required, following the emergence of resistance to the most used drugs. Natural products contribute greatly to the therapeutic arsenal in this area, including artemisinin and quinine (and atovaquone, semi-synthetic). Among the natural products, indole alkaloids represent an interesting class of compounds. Screening carried out to date has revealed several substances active in vitro under the micromolar range and with a good selectivity index. This review covers the indole alkaloids with high antiplasmodial activity (in vitro and in vivo) isolated from natural sources, and is organized according to the different chemical structures of the alkaloids. [less ▲]

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See detailEvaluation of medicinal plants from Reunion Island for antimalarial and cytotoxic activities
Jonville, Marie ULg; Kodja, H.; Humeau, L. et al

in Planta Medica (2008), 74(9), 1002-1002

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See detailIn vitro antiplasmodial activity of ethnobotanically selected plants from Burkina Faso
Jansen, Olivia ULg; Angenot, Luc ULg; Tits, Monique ULg et al

in Planta Medica (2008), 74(9), 1142-1142

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See detailScreening of medicinal plants from Reunion Island for antimalarial and cytotoxic activity.
Jonville, Marie ULg; Kodja, H.; Humeau, L. et al

in Journal of Ethnopharmacology (2008), 120(3), 382-6

AIM OF THE STUDY: Nine plants from Reunion Island, selected using ethnopharmacology and chemotaxonomy, were investigated for their potential antimalarial value. MATERIALS AND METHODS: Thirty-eight ... [more ▼]

AIM OF THE STUDY: Nine plants from Reunion Island, selected using ethnopharmacology and chemotaxonomy, were investigated for their potential antimalarial value. MATERIALS AND METHODS: Thirty-eight extracts were prepared by maceration using CH(2)Cl(2) and MeOH, and were tested for in vitro activity against the 3D7 and W2 strain of Plasmodium falciparum. The most active extracts were then tested for in vitro cytotoxicity on human WI-38 fibroblasts to determine the selectivity index. Those extracts were also investigated in vivo against Plasmodium berghei infected mice. RESULTS: Most active of the extracts tested were the dichloromethane leaves extracts of Nuxia verticillata Lam. (Buddlejaceae), Psiadia arguta Voigt. (Asteraceae), Lantana camara L. (Verbenaceae), the methanol extracts from Aphloia theiformis (Vahl) Benn. (Aphloiaceae) bark, and Terminalia bentzoe L. (Combretaceae) leaves displaying in vitro IC(50) values ranging from 5.7 to 14.1mug/ml. Extracts from Psiadia, Aphloia at 200mg/(kgday) and Teminalia at 50mg/(kgday) also exhibited significant (p<0.0005) parasite inhibition in mice: 75.5%, 65.6% and 83.5%, respectively. CONCLUSION: Two plants showed interesting antimalarial activity with good selectivity: Aphloia theiformis and Terminalia bentzoe. Nuxia verticillata still needs to be tested in vivo, with a new batch of plant material. [less ▲]

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See detailComponents of Haplophyllum pedicellatum
Akhmedjanova, V. I.; Shashkov, A. S.; Angenot, Luc ULg et al

in Chemistry of Natural Compounds (2008), 44(5), 646-647

We investigated the aerial parts of Haplophyllum pedicellatum collected in the district of Samarkand (Uzbekistan). We have separated and identified 11 components, of which 7 compounds including the ... [more ▼]

We investigated the aerial parts of Haplophyllum pedicellatum collected in the district of Samarkand (Uzbekistan). We have separated and identified 11 components, of which 7 compounds including the alkaloids acetylevoxine, glycoperine and glucohaplopine, all three isolated lignans (suchilactone, diphyllin and justicidin B) and the flavonoid glycoside ( limocitrin 7-O-beta-glucoside) were observed for the first time in the aerial part of H. pedicellatum.The structures were established by comparison with standars and with spectral data. the cytotoxic activity was determined : the flavonoid exhibited a moderate activity. [less ▲]

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See detailIn vitro cytotoxicity of some medicinal plants from Georgian amaryllidaceae
Jokhadze, Malkhaz; Eristavi, Lina; Kutchukhidze, Jumber et al

in Phytotherapy Research (2007), 21(7), 622-624

Using an ethnomedical data approach, some Georgian plants, which are used in Georgian traditional medicine for cancer or non-cancer diseases, were collected and evaluated for cytotoxic activity. The ... [more ▼]

Using an ethnomedical data approach, some Georgian plants, which are used in Georgian traditional medicine for cancer or non-cancer diseases, were collected and evaluated for cytotoxic activity. The cytotoxic effect of the methanol extracts of species from the genera Galanthus and Leucojum was evaluated in vitro on three human cell lines (Hela, ephitheloid cervix carcinoma; HCT-116, colon carcinoma; HL-60, acute myeloid leukaemia). Cell type cytotoxic specificity was observed for some extracts. Overall, the HCT-116 cells were much more sensitive to most of the extracts than were the other cell lines. Plants that showed pronounced cytotoxic activity will be further evaluated for the possible isolation of active antitumour compounds. Copyright (C) 2007 John Wiley [less ▲]

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