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See detailApplication of a new optimization strategy for the separation of tertiary alkaloids extracted from Strychnos usambarensis leaves
Nistor, Iolanda ULg; Cao, Martine ULg; Debrus, Benjamin ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (2011), 56

The HPLC separation of six alkaloids extracted from Strychnos usambarensis leaves has been developed and optimized by means of a powerful methodology for modelling chromatographic responses, based on ... [more ▼]

The HPLC separation of six alkaloids extracted from Strychnos usambarensis leaves has been developed and optimized by means of a powerful methodology for modelling chromatographic responses, based on three steps, i.e. design of experiments (DoE), independent component analysis (ICA) and design space (DS). This study was the first application of a new optimization strategy to a complex natural matrix. The compounds separated are the isomers isostrychnopentamine and strychnopentamine, 10-hydroxyusambarine and 11-hydroxyusambarine, also strychnophylline and strychnofoline. Three LC parameters have been optimized using a multifactorial design comprising 29 experiments that includes 2 center point replicates. The parameters were the percentage of organic modifiers used at the beginning of a gradient profile which consisted in different proportions of methanol (MeOH) and acetonitrile (MeCN), the gradient time to reach 70% of organic modifiers starting from the initial percentage and the percentage of MeCN found in the mobile phase. Subsequent to the experimental design application, predictive multilinear models were developed and used in order to provide optimal analytical conditions. The optimum assay conditions were: methanol/acetonitrile-sodium pentane sulfonate (pH 2.2; 7.5 mM) (33.4:66.6, v/v) at a mobile phase flow rate of 1mL/min during a 40.6 minutes gradient time. The initial organic phase contained 3.7% MeCN and 96.3% MeOH. The method showed good agreement between the experimental data and predictive value throughout the studied parameters space. Improvement of the analysis time and optimized separation for the compounds of interest was possible due to the original and powerful tools applied. Finally, this study permitted the acquisition of isomers profiles allowing the identification of the optimal collecting period of Strychnos usambarensis. [less ▲]

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See detailAntiplasmodial, anti-inflammatory and cytotoxic activities of various plant extracts from the Mascarene Archipelago.
Jonville, Marie ULg; Kodja, H.; Strasberg, D. et al

in Journal of Ethnopharmacology (2011), 136

AIM OF THE STUDY: Antiplasmodial activity, inhibition of nitric oxide (NO) overproduction, and anti-proliferative activity were investigated in vitro to evaluate the bioactive potential of the traditional ... [more ▼]

AIM OF THE STUDY: Antiplasmodial activity, inhibition of nitric oxide (NO) overproduction, and anti-proliferative activity were investigated in vitro to evaluate the bioactive potential of the traditional pharmacopoeia of the Mascarene Archipelago, which is known for its biodiversity and for the richness of its endemic flora. MATERIALS AND METHODS: A total of 45 methanol (MeOH) and dichloromethane (DCM) extracts were prepared from 19 plant species collected on Reunion and Mauritius Islands. Ninety-six-well microplate assays were performed on chloroquine sensitive Plasmodium falciparum 3D7 strain, on LPS-stimulated Raw 264.7 murine macrophages and on A-549, DLD-1 and WS1 human cells. Activity was evaluated through spectrophotometric methods. RESULTS: Activity was attributed to plant extracts expressing IC(50)<50mug/ml for antiplasmodial response, IC(50)<100mug/ml for cytotoxicity, and IC(50)<130mug/ml for anti-inflammatory reaction. The majority of the extracts tested (69%) exhibited potency in at least one of these three types of activity. This is the first report describing promising antiplasmodial activity (IC(50)<15mug/ml) for Psiadia dentata DCM extract and Terminalia bentzoe MeOH bark extract. NO inhibition assay revealed seven interesting plants, described for the first time as anti-inflammatory: Aphloia theiformis, Buddleja salviifolia, Eupatorium riparium, Hiptage benghalensis, Psiadia arguta, Psiadia dentata, and Scutia commersonii. Finally, anti-proliferative activity was observed for two endemic species, Geniostoma borbonicum and Nuxia verticillata. CONCLUSION: Using the criterion of endemism as part of the criteria for traditional medicinal use raises the chances of finding original active principles. In our case, 86% of the endemic plants tested displayed pharmacological interest. [less ▲]

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See detailQuality Assessment of Polygonum cuspidatum and Polygonum multiflorum by 1H NMR Metabolite Fingerprinting and Profiling Analysis.
Frederich, Michel ULg; Wauters, Jean-Noël ULg; Tits, Monique ULg et al

in Planta Medica (2011), 77

The quality assessment and control of traditional Chinese medicines (TCM) nowadays receives a great deal of attention worldwide and particularly in Europe with its increasing local use. POLYGONUM ... [more ▼]

The quality assessment and control of traditional Chinese medicines (TCM) nowadays receives a great deal of attention worldwide and particularly in Europe with its increasing local use. POLYGONUM CUSPIDATUM Siebold & Zucc. and POLYGONUM MULTIFLORUM Thunb. are two members of the Polygonaceae family, which are widely used as Chinese medicinal plants. The aim of this study was to achieve an overview of the quality of P. CUSPIDATUM and P. MULTIFLORUM samples available on the Chinese market and to identify important metabolites for their discrimination, using (1)H NMR-based metabolomics. (1)H NMR and multivariate analysis techniques were applied to almost 60 plant samples collected in different places in China. Using (1)H NMR metabolomics, it was possible, without previous evaporation or separation steps, to obtain metabolic fingerprints to distinguish between the species. The important metabolites for discrimination were stilbene derivatives. Finally, a clear distinction between the two species was possible and the discriminant metabolites were identified. [less ▲]

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See detailLC-SPE-NMR-MS analysis of Strychnos usambarensis fruits from Rwanda
Cao, Martine ULg; Tits, Monique ULg; Muganga, Raymond et al

in Planta Medica (2010, September), 76(12), 1241-1242

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See detailPharmacognosie volume I
Frederich, Michel ULg; Tits, Monique ULg; Angenot, Luc ULg

Learning material (2010)

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See detailAntioxidant activity of Passiflora edulis and Passiflora alata fruits
Yariwake, J.; Zeraik, M.; Serteyn, Didier ULg et al

in Planta Medica (2010, September), 76(12), 1274-1275

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See detailBioassay-guided isolation of antiplasmodial Strychnos alkaloids from the stem-bark of Strychnos icaja BAILLON
Tchinda Tiabou, Alembert ULg; Tamze, Victorine; Frederich, Michel ULg et al

in Planta Medica (2010, September), 76(12), 1305

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See detailAnti-inflammatory potency of the traditionally used antimalarial plant Fagraea fragrans
Jonville, Marie ULg; Baghdikian, Béatrice; Ollivier, Evelyne et al

in Planta Medica (2010, September), 76(12), 1171

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See detailPharmacognosie - Volume 2
Angenot, Luc ULg; Tits, Monique ULg; Frederich, Michel ULg

Learning material (2010)

Le volume 2 est consacré aux grands classiques de la pharmacognosie dont ont été isolés de très nombreux principes actifs (souvent des alcaloïdes) qui ont marqué l'histoire de la pharmacie et de la ... [more ▼]

Le volume 2 est consacré aux grands classiques de la pharmacognosie dont ont été isolés de très nombreux principes actifs (souvent des alcaloïdes) qui ont marqué l'histoire de la pharmacie et de la pharmacologie.Les chapitres suivants seront abordés: analgésiques (pavot,opium, colchique...); Solanacées à activité parasympatholytiques ( belladone, stramoine, jusquiames...); dérivés de l'ergot de seigle; médicaments des troubles cérébraux de la sénescence ( ginkgo, amaryllidacées à galanthamine...); drogues alcaloïdiques psychoactives ( coca, éphédra, khat, psilocybes, peyotl, harmel, yagé, iboga...); hallucinogènes non alcaloïdiques ( cannabis, sauge des devins..); poisons agissant sur la neurotransmission :cholinergiques ( jaborandi, éséré...), paralysants neuro-musculaires (curares) et antagonistes de la glycine ( noix-vomique); antiparasitaires (malaria et amibiase): quinquinas, armoises, ipécas; anticancéreux : lignanes ( Podophyllum), diterpènes des ifs (Taxus sp), alcaloïdes indoliques ( Catharanthus roseus, Camptotheca ...), divers ( origine marine, épices...); plantes toxiques de l'environnement ( toxicité par contact ou par ingestion) [less ▲]

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See detailPharmacognosie Volume 3
Angenot, Luc ULg; Tits, Monique ULg; Frederich, Michel ULg

Learning material (2010)

Le volume 3 est principalement consacré à la phytothérapie (intérêt et limites) avec des notions concernant aussi bien la médecine traditionnelle que les développements les plus récents ( y compris pour ... [more ▼]

Le volume 3 est principalement consacré à la phytothérapie (intérêt et limites) avec des notions concernant aussi bien la médecine traditionnelle que les développements les plus récents ( y compris pour certains s'appuyant sur des études cliniques) en matière de phytomédicaments. La classification se fait suivant les activités pharmacologiques de ces plantes. Au terme de ce cours, les étudiants doivent pouvoir faire la distinction entre les différentes formes pharmaceutiques à base de produits d'origine naturelle ( poudres, extraits sec et autres), les concentrations en principes actifs et traceurs y étant bien différentes. Les différences entre les médicaments enregistrés et les compléments alimentaires sont également bien mises en évidence car les exigences relatives au contrôle de qualité et à la stabilité des produits finis sont très différentes. A ce sujet l'annexe du volume 3 est consacrée au contrôle de qualité des plantes en insistant sur les méthodes et monographies de la pharmacopée européenne qui joue un rôle essentiel dans ce domaine. [less ▲]

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See detailMetabolomic analysis of Echinacea spp. by (1)H nuclear magnetic resonance spectrometry and multivariate data analysis technique.
Frederich, Michel ULg; Jansen, C.; De Tullio, Pascal ULg et al

in Phytochemical Analysis (2010), 21(1), 61-65

Introduction - The genus Echinacea (Asteraceae) comprises about 10 species originally distributed in North America. Three species are very well known as they are used worldwide as medicinal plants ... [more ▼]

Introduction - The genus Echinacea (Asteraceae) comprises about 10 species originally distributed in North America. Three species are very well known as they are used worldwide as medicinal plants: Echinacea purpurea, E. pallida, E. angustifolia.Objective - To discriminate between these three Echinacea species and E. simulata by (1)H NMR-based metabolomics.Methodology - (1)H NMR and multivariate analysis techniques were applied to diverse Echinacea plants including roots and aerial parts, authentic plants, commercial plants and commercial dry extracts.Results - Using the (1)H NMR metabolomics, it was possible, without previous evaporation or separation steps, to obtain a metabolic fingerprint to distinguish between species.Conclusion - A clear distinction between the three pharmaceutical species was possible and some useful metabolites were identified. Copyright (c) 2009 John Wiley & Sons, Ltd. [less ▲]

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See detailMoandaensine, a dimeric indole alkaloid from Strychnos moandaensis (Loganiaceae)
Verpoorte, Robert; Frederich, Michel ULg; Delaude, Clément et al

in Phytochemistry Letters (2010), 3

Moandaensine contains a rare anhydronium base subunit. It presents a moderate antiplasmodial activity with IC values of 11.2 microM and 9.2 microM against , respectively, the chloroquino sensitive FCA 20 ... [more ▼]

Moandaensine contains a rare anhydronium base subunit. It presents a moderate antiplasmodial activity with IC values of 11.2 microM and 9.2 microM against , respectively, the chloroquino sensitive FCA 20 GHA and chloroquino resistant W2 strains of Plasmodium falciparum [less ▲]

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See detailAntiplasmodial and cytotoxic activities of Rwandan medicinal plants used in the treatment of malaria.
Muganga, R.; Angenot, Luc ULg; Tits, Monique ULg et al

in Journal of Ethnopharmacology (2010), 128

AIM OF THE STUDY: In our study, methanol, dichloromethane and aqueous extracts of 13 Rwandan medicinal plants used in the treatment of malaria were tested for in vitro antiplasmodial activity. MATERIALS ... [more ▼]

AIM OF THE STUDY: In our study, methanol, dichloromethane and aqueous extracts of 13 Rwandan medicinal plants used in the treatment of malaria were tested for in vitro antiplasmodial activity. MATERIALS AND METHODS: The growth inhibition of chloroquine-sensitive Plasmodium falciparum strain (3D7) was evaluated using the measurement of lactate dehydrogenase activity. The active extracts were also tested against the chloroquine-resistant Plasmodium falciparum strain (W2) and for cytotoxicity assay using human normal foetal lung fibroblasts (WI-38). RESULTS: The majority of the plants tested showed an antiplasmodial activity and the best results were observed with dichloromethane leaf and flower extracts of Tithonia diversifolia, leaf extract of Microglossa pyrifolia and root extract of Rumex abyssinicus, methanol leaf extract of Fuerstia africana, root bark extracts of Zanthoxylum chalybeum and methanol bark extract of Terminalia mollis. Those extracts were active (IC(50)<15mug/ml) on both chloroquine-sensitive and resistant strains of Plasmodium falciparum. Zanthoxylum chalybeum, Solanecio mannii and Terminalia mollis presented the best selectivity index. CONCLUSIONS: The traditional use of most of the plant evaluated was confirmed by the antiplasmodial test. This study revealed for the first time the antiplasmodial activity of two plants: Terminalia mollis and Rumex abyssinicus. [less ▲]

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See detailIsostrychnopentamine, an Indolomonoterpenic Alkaloid from Strychnos usambarensis, with Potential Antitumor Activity against Apoptosis-Resistant Cancer Cells
Balde, El-Hadj Saidou; Mégalizzi, Véronique; Angenot, Luc ULg et al

in International Journal of Oncology (2010), 36

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See detailEvaluation of 13 selected medicinal plants from Burkina Faso for their antiplasmodial properties.
Jansen, Olivia ULg; Angenot, Luc ULg; Tits, Monique ULg et al

in Journal of Ethnopharmacology (2010), 130

AIM OF THE STUDY: The aim of this study was to evaluate the antiplasmodial properties of 13 plants used against malaria in traditional medicine in Burkina Faso. MATERIALS AND METHODS: In vitro ... [more ▼]

AIM OF THE STUDY: The aim of this study was to evaluate the antiplasmodial properties of 13 plants used against malaria in traditional medicine in Burkina Faso. MATERIALS AND METHODS: In vitro antiplasmodial activity of dichloromethane, methanol and aqueous crude extracts obtained from vegetal samples collected in Burkina Faso was first evaluated on the Plasmodium falciparum 3D7 chloroquine-sensitive strain using a colorimetric method. RESULTS: Thirteen extracts obtained from 8 different species were found to exhibit antiplasmodial activity (IC(50)<50mug/ml). Five species demonstrated a moderate activity (15mug/ml<IC(50)<50mug/ml): Boswellia dalzielii (leaves), Waltheria indica (roots and aerial parts), Bergia suffruticosa (whole plant), Vitellaria paradoxa (bark) and Jatropha gossypiifolia (leaves). The best results were obtained with extracts from the Dicoma tomentosa whole plant, from Psorospermum senegalense leaves and from Gardenia sokotensis leaves. These extracts found to display promising antiplasmodial activity, with IC(50) values ranging from 7.0 to 14.0mug/ml. The most active plant extracts were then tested for in vitro activity on the Plasmodium falciparum W2 chloroquine-resistant strain and also for in vitro cytotoxicity on normal human fibroblasts (WI-38) in order to determine the selectivity index. CONCLUSIONS: Dicoma tomentosa (Asteraceae) and Psorospermum senegalense (Clusiaceae) appeared to be the best candidates for further investigation of their antiplasmodial properties, reported for the first time by this study. [less ▲]

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See detailAlkaloids from Haplophyllum leptomerum
Akhedzhanova, V. I.; Angenot, Luc ULg; Shakirov, R.Sh.

in Chemistry of Natural Compounds (2010), 46(3), 502-503

The plant Haplophyllum leptomerum is indigenous to Uzbekistan and produces alkaloids. This paper describes the isolation and structure determination of 7 alkaloids. Herein we report also the anticancer ... [more ▼]

The plant Haplophyllum leptomerum is indigenous to Uzbekistan and produces alkaloids. This paper describes the isolation and structure determination of 7 alkaloids. Herein we report also the anticancer properties of dictamnine that exhibited moderate activity against two human cancer lines : HeLa and HCT-116. [less ▲]

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