References of "Angenot, Luc"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailDensitometric Evaluation of Gentiopicroside in the Roots of Gentiana lutea L.
Bodart, Patricia; Poukens-Renwart, Pascale; Wauters, Jean-Noël ULg et al

in Journal of Planar Chromatography- Modern TLC (1996), 9(March/April), 143-145

As no satisfactory method existed for the separation of gentiopicroside from other components of gentian roots, we have decided to undertake some experiments.In this communication, we report the use of a ... [more ▼]

As no satisfactory method existed for the separation of gentiopicroside from other components of gentian roots, we have decided to undertake some experiments.In this communication, we report the use of a novel mobile phase and a different detection system. Our HPTLC method is a fast and reliable procedure which may be used for quantification of gentiopicroside in roots and extracts of Gentiana lutea, whether for routine analysis or for studying stability. The TLC method is also compared with an HPLC method. [less ▲]

Detailed reference viewed: 62 (1 ULg)
Full Text
Peer Reviewed
See detailIn Vitro Cytotoxic Activity of Two Potential Anticancer Drugs Isolated from Strychnos: Strychnopentamine and Usambarensine
Bonjean, K. A.; Gillet, Marie-Claire ULg; Quetin-Leclercq, J. et al

in Anticancer Research (1996), 16(3A, May-Jun), 1129-37

The cytotoxicity and the selective antiprotozoal activity of some Strychnos alkaloids, namely strychnopentamine (SP) and usambarensine (US) (7) led us to analyze and compare their effects with emetine (EM ... [more ▼]

The cytotoxicity and the selective antiprotozoal activity of some Strychnos alkaloids, namely strychnopentamine (SP) and usambarensine (US) (7) led us to analyze and compare their effects with emetine (EM) by using mouse B16 melanoma cells cultivated in vitro. We observed by cytological analysis and proliferation rate studies that these substances induce analogous cytotoxic effects in B16 cells, but at different concentrations i.e. formation of lamellar bodies in the cytoplasm, the which contain pre-melanosomes in the case of SP and US, vacuoles and blebs. At concentrations near their respective IC50, SP and US, but not EM, decreased colony formation. We showed by incorporation of labelled precursors that SP and US first inhibit RNA synthesis while EM initially acts on protein synthesis. These alkaloids increased melanin synthesis. Furthermore, only EM and SP caused hemolysis of sheep red blood corpuscles. This could explain why the rate of antiplasmodial activity is higher for SP and EM. [less ▲]

Detailed reference viewed: 9 (3 ULg)
Full Text
Peer Reviewed
See detailA new HPLC method for the assay of alkaloids in Strychnos nux-vomica and Strychnos ignatii
Gadi Biala, Rose; Tits, Monique ULg; Wauters, Jean-Noël ULg et al

in Fitoterapia (1996), 67(2), 163-165

A reversed-phase high performance liquid chromatographic procedure was developed for the qualitative and quantitative analysis of the alkaloids in S. nux-vomica and S. ignatii.

Detailed reference viewed: 51 (8 ULg)
Full Text
Peer Reviewed
See detailAntimalarial Activity of Cryptolepine and some otrher Anhydronium Bases
Wright, C. W.; Phillipson, J. D.; Awe, S. O. et al

in Phytotherapy Research (1996), 10

Eight naturally occurring anhydronium bases and the synthetic quaternary compound Nb-methylharmalane were tested against Plasmodium falciparun (strain K1) in vitro. Cryptolepine was found to have similar ... [more ▼]

Eight naturally occurring anhydronium bases and the synthetic quaternary compound Nb-methylharmalane were tested against Plasmodium falciparun (strain K1) in vitro. Cryptolepine was found to have similar activity to that of chloroquine but alstonine, 5,6-dihydroflavopereirine, matadine, Nb-methylharmalane, melinonine F, normelinonine F, strychnoxanthine and serpentine were found to have little activity. Cryptolepine, given orally to mice infected with Plasmodium berghei berghei was found to have moderate antimalarial activity; parasitemia was suppressed by 80% at 50 mg/kg/day. [less ▲]

Detailed reference viewed: 20 (2 ULg)
Full Text
Peer Reviewed
See detailQuantitative Densitometric Evaluation of Harpagoside in the Secondary Roots of Harpagophytum procumbens D.C.
Poukens-Renwart, Pascale; Tits, Monique ULg; Angenot, Luc ULg

in Journal of Planar Chromatography- Modern TLC (1996), 9(May/June), 199-202

Harpagophytum procumbens D.C. (Devil's Claw) secondary roots are used in Europe as a home remedy for lack of appetite, dyspeptic complaints, and in supportive therapy for degenerative disorders of the ... [more ▼]

Harpagophytum procumbens D.C. (Devil's Claw) secondary roots are used in Europe as a home remedy for lack of appetite, dyspeptic complaints, and in supportive therapy for degenerative disorders of the locomotor system. The main characteristic iridoid glucoside of the plant is harapagoside. An HPTLC method has been developed for the quantitative determination of harpagoside in the secondary roots and extracts of this plant. The mobile phase used is an optimized version of one described in the literature.It furnished the most homogenous spots for quantitative densitometric evaluation ( at lambda= 286 nm, the wavelenght of maximum absorption for harapgoside).The results have been compared with those obtained afetr visualization with sulfuric acid-vanillin reagent and those obtained using a previously published HPLC method; To check that separation was complete, the spectra of harpagoside obtained from Devil's Claw extracts before and after visualization were superimposed on thos of harpagoside standard. [less ▲]

Detailed reference viewed: 42 (0 ULg)
Full Text
Peer Reviewed
See detailCritical Evaluation of the DNA-methyl Green Assay: Application to Some Indole Alkaloids
Bonjean, K.; Gillet, Marie-Claire ULg; Bassleer, R. et al

in Phytotherapy Research (1996), 10

A DNA-methyl green colorimetric microassay for the detection of agents that interact with DNA was assessed with reference molecules (doxorubicin, ellipticine, melinonine F, dihydroflavopereirine, cis-Pt ... [more ▼]

A DNA-methyl green colorimetric microassay for the detection of agents that interact with DNA was assessed with reference molecules (doxorubicin, ellipticine, melinonine F, dihydroflavopereirine, cis-Pt, mitomycin C, emetine or colchicine). This test showed some promise as a screening technique for substances that intercalate partly or totally into the DNA helix but it is not suitable for substances that bind covalently to DNA. An application of this test to a number of indole alkaloids was also performed. Cryptolepine, serpentine, alstonine and matadine induced a reduction of the colour of the solution, so indicating an interaction with DNA. [less ▲]

Detailed reference viewed: 156 (2 ULg)
Full Text
See detailPharmacologie - Suivez la Flèche
Lambert, Philippe; Angenot, Luc ULg

Article for general public (1995)

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailGuianensine, a zwitterionic alkaloid from Strychnos guianensis
Quetin-Leclercq, Joëlle; Llabres, Gabriel ULg; Warin, Roger et al

in Phytochemistry (1995), 40(5), 1557-1560

The isolation and structure determination of an alkaloid isolated from the stem bark of Strychnos guianensis is described. Elucidation of its structure is based mainly on 1D and 2D NMR studies. The new ... [more ▼]

The isolation and structure determination of an alkaloid isolated from the stem bark of Strychnos guianensis is described. Elucidation of its structure is based mainly on 1D and 2D NMR studies. The new alkaloid has a zwitterionic asymmetrical bis-indole structure and is named guianensine. [less ▲]

Detailed reference viewed: 33 (3 ULg)
Full Text
Peer Reviewed
See detailScreening for in vitro Antifungal Activities of some Indole Alkaloids
Quetin-Leclercq, J.; Favel, A.; Balansard, G. et al

in Planta Medica (1995), 61

Thirty indole alkaloids were tested by the agar dilution technique on a panel of human pathogenic fungi, yeasts and dermatophytes. Our results indicate that the most active compounds possess a beta ... [more ▼]

Thirty indole alkaloids were tested by the agar dilution technique on a panel of human pathogenic fungi, yeasts and dermatophytes. Our results indicate that the most active compounds possess a beta-carboline skeleton and that the presence of a 3,4 double bond enhances the activity. Our results also show that antifungal activities are not linked to the cytotoxic, antimicrobial or antiparsitic properties. [less ▲]

Detailed reference viewed: 24 (2 ULg)
Full Text
See detailUtilisation de prodelphinidines pour le traitement de l'arthrose
Franchimont, Paul; Bassleer, Corine; Angenot, Luc ULg et al

Patent (1994)

The subject of the invention is the use of prodelphinidins for obtaining medicaments intended for the treatment of articular pathologies associated with the destruction of the cartilage, and more ... [more ▼]

The subject of the invention is the use of prodelphinidins for obtaining medicaments intended for the treatment of articular pathologies associated with the destruction of the cartilage, and more particularly of arthrosis. [less ▲]

Detailed reference viewed: 33 (7 ULg)
Full Text
See detailActivité antiinflammatoire du mucilage de bleuet
Bodart, Patricia; Tits, Monique ULg; Angenot, Luc ULg

Poster (1994, May)

Detailed reference viewed: 4 (1 ULg)
Full Text
Peer Reviewed
See detailOn the occurrence of (iso-)dolichantoside in Strychnos mellodora
Quarré, Marie-Noëlle; Tits, Monique ULg; Wauters, Jean-Noël ULg et al

Poster (1994, March)

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailAntiinflammatory activity of centaurea cyanus flowers
Bodart, Patricia; Goldsztajn, V.; Tits, Monique ULg et al

Poster (1994, March)

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailAntiinflammatory activity of Centaurea cyanus flowers
Bodart, Patricia; Damas, Jacques ULg; Goldsztajn, V. et al

in Journal de Pharmacie de Belgique (1994, February), 59(1), 73

Detailed reference viewed: 19 (1 ULg)
Full Text
Peer Reviewed
See detailEffects of Alpha-Hederin, a Saponin Extracted from Hedera Helix, on Cells Cultured in Vitro
Danloy, S.; Quetin-Leclercq, J.; Coucke, P. et al

in Planta Medica (1994), 60(1), 45-9

In this work, we have analysed the effects of alpha-hederin, a monodesmosidic triterpenoid saponin isolated from Hedera helix, on mouse B16 melanoma cells and non-cancer mouse 3T3 fibroblasts cultured in ... [more ▼]

In this work, we have analysed the effects of alpha-hederin, a monodesmosidic triterpenoid saponin isolated from Hedera helix, on mouse B16 melanoma cells and non-cancer mouse 3T3 fibroblasts cultured in vitro. Our results indicate that, in a serum-free medium, alpha-hederin is cytotoxic and inhibits proliferation in both cell lines at rather low concentrations (< 5 micrograms/ml) after only 8 hours of treatment. Its cytotoxicity decreases in the presence of serum in which BSA seems to be able to bind the saponin. alpha-Hederin also induces vacuolization of the cytoplasm and membrane alterations leading to cell death. [less ▲]

Detailed reference viewed: 81 (2 ULg)
Full Text
Peer Reviewed
See detail2,7-dihydroapogeissoschizine from root bark of strychnos Gossweileri
Quetin-Leclercq, Joëlle; Dive, Georges ULg; Delaude, Clément et al

in Phytochemistry (1994), 35(2), 533-536

In this paper, we describe the isolation and structural determination of 2,7-dihydroxyapogeissoschizine, a new alkaloid from the root bark of Strychnos gossweileri. Elucidation of its structure is based ... [more ▼]

In this paper, we describe the isolation and structural determination of 2,7-dihydroxyapogeissoschizine, a new alkaloid from the root bark of Strychnos gossweileri. Elucidation of its structure is based mainly on 1D and 2D NMR studies; its conformation was optimized by energy minimization. This type of skeleton is related to geissoschizine but with the notable diffrence that the 1 and 17 positions are joined giving an additional fused ring. Such a seven-membered ring has ,to our knowledge, only been observed in apogeissoschizine, obtained after chemical tratment of two alkaloids ( geissospermine and geissoschizine). 2,7-dihydroxyapogeissoschizine is therefore, the first natural product possessing this skeleton. According to a preliminary test, this new alkaloid shows low toxicity to B16 melanoma cells, but not for non-cancer 3T3 fibroblasts cultured in vitro.THese results have to be confirmed by further tests. [less ▲]

Detailed reference viewed: 34 (5 ULg)
Full Text
Peer Reviewed
See detailSelective Antiprotozoal Activity of Some Strychnos Alkaloids
Wright, Colin; Allen, David; Cai, Ya et al

in Phytotherapy Research (1994), 8

The in vitro activities of 28 Strychnos alkaloids have been determined against three species of protozoa and the most active compounds were then assessed for in vitro cytotoxic activity against KB cells ... [more ▼]

The in vitro activities of 28 Strychnos alkaloids have been determined against three species of protozoa and the most active compounds were then assessed for in vitro cytotoxic activity against KB cells. 3',4'-dihydrousambarensine was the most active against Plasmodium falciparum and was relatively nontoxic to KB cells (cytotoxic/antiplasmodial ratio, 1474). Usambarensine displayed the most selectivity against Entamoeba histolytica while "',4'-dihydrousambarensine was the most selective compound against Giardia intestinalis although the cytotoxic/antiprotozoal ratios (20.4 and 6.6 respectively) were less favourable than the above.The four strychnobiline-type alkaloids were found devoid of activity against E. histolytica and G. intestinalis but to have weak antiplasmodial properties (7-13 times less active than chloroquine). None of the compounds containing only one indole ring system displayed antiprotozoal properties. [less ▲]

Detailed reference viewed: 30 (1 ULg)
Full Text
Peer Reviewed
See detailStrychnopentamine, a Potential Anticancer Agent
Quetin-Leclercq, J.; Bouzahzah, B.; Pons, A. et al

in Planta Medica (1993), 59(1), 59-62

We analysed the effects of strychnopentamine, an alkaloid isolated from Strychnos usambarensis, on an Ehrlich ascites tumor growing in the mouse after inoculation. Four subcutaneous injections of 1.5 mg ... [more ▼]

We analysed the effects of strychnopentamine, an alkaloid isolated from Strychnos usambarensis, on an Ehrlich ascites tumor growing in the mouse after inoculation. Four subcutaneous injections of 1.5 mg strychnopentamine (1 per day) induce a significant decrease of the number of tumor cells and a significant increase of the survival of the treated mice. Observed side effects are partial haemolysis and some liver damage. [less ▲]

Detailed reference viewed: 18 (4 ULg)
Full Text
Peer Reviewed
See detailSemi-Quantitative Salicin Analysis According the German Pharmacopoeia (DABX): Avoidance of Picein Interference
Poukens-Renwart, Pascale; Tits, Monique ULg; Angenot, Luc ULg

in Planta Medica (1993), 59(Supplement Issue), 629-630

The semi-quantitative determination of salicin by TLC described in the German Pharmacopoeia (DABX) does not separate salicin from picein, another phenolic glycoside of some willow species. In this paper ... [more ▼]

The semi-quantitative determination of salicin by TLC described in the German Pharmacopoeia (DABX) does not separate salicin from picein, another phenolic glycoside of some willow species. In this paper we propose to combine the use of both an adequate mobile phase and a suitable reagent (vanillin reagent instead of sulfuric acid-thymol) for an unambiguous determination of the amount of salicin.. [less ▲]

Detailed reference viewed: 81 (0 ULg)
Full Text
Peer Reviewed
See detailDensitometric Determination of Salicin in Willow Stem Bark
Poukens-Renwart, Pascale; Tits, Monique ULg; Angenot, Luc ULg

in Journal of Planar Chromatography (1993), 6(november-december), 434-437

Most of the salicin present in willow stem bark occurs as labile glycosides (e.g. salicin, salicortin, 2-O'-acetylsalicortin, 3-O'-acetylsalicortin, tremulacin and fragilin). On alkaline hydrolysis, these ... [more ▼]

Most of the salicin present in willow stem bark occurs as labile glycosides (e.g. salicin, salicortin, 2-O'-acetylsalicortin, 3-O'-acetylsalicortin, tremulacin and fragilin). On alkaline hydrolysis, these acylated derivatives decompose to salicin; in this way, the total amount of salicin can be determined. A HPTLC method has been developed for the quantitative determination of salicin in stem bark before and after alkaline hydrolysis. The mobile phase used for the separation is a modification of one described in the literature. a double or triple migration is necessary to separate salicin from other components of the extract. The results have been compared with those obtained after bidimensional chromatography using two phases described for the quantitative evaluation of salicin. to check that separation was complete, the spectrum of salicin obtained from willow extracts was superimposed on that of a salicin standard.Quantification was performed at lambda = 270nm, the wavelenght of maximum absorption in the IV spectrum of salicin. [less ▲]

Detailed reference viewed: 150 (14 ULg)