Proanthocyanidins from Ribes nigrum leaves : TLC Analysis

in Bulletin de Liaison - Groupe Polyphenols (1992)

In summary, the main oligomeric prodelphinidins of Ribes nigrum leaves can be identified by HPTLC; this method could also be utilized as a proanthocyanidin screen for determining their occurrence in other ... [more ▼]

In summary, the main oligomeric prodelphinidins of Ribes nigrum leaves can be identified by HPTLC; this method could also be utilized as a proanthocyanidin screen for determining their occurrence in other plants and for quantification of the major dimers. [less ▲]

Proanthocyanidins from Ribes nigrum leaves - Isolation and structure determination

in Bulletin de Liaison Groupe Polyphenols (1992)

In summary, the antiinflammatory activity of Ribes nigrum leaves is mainly due to proanthocyanidins which are the major oligomers in the proanthocyanidins fraction

Reversed-Phase Hptlc Densitometric Evaluation of Fraxin in Fraxinus Excelsior Leaves

in Journal of Pharmaceutical & Biomedical Analysis (1992), 10(10-12, Oct-Dec), 1089-91

The present work describes a rapid and selective assay for fraxin based on its characteristic fluorescent properties by densitometry on reversed-phase HPTLC plates. Two chromatographgic procedures ... [more ▼]

The present work describes a rapid and selective assay for fraxin based on its characteristic fluorescent properties by densitometry on reversed-phase HPTLC plates. Two chromatographgic procedures (Silicagel and RP 18) have been assessed and the results compared to those obtained by reversed-phase HPLC on ODS-silica. The amount of fraxin found in six commercial batches of Fraxinus excelsior leaves ( based on dry weight) ranged from 0.1 to 0.2%. [less ▲]

Densitometric Evaluation of Spiraeoside after Derivatization in Flowers of Filipendula Ulmaria (L.) Maxim

in Journal of Pharmaceutical & Biomedical Analysis (1992), 10(10-12, Oct-Dec), 1085-8

Spiraeoside (quercetin-4'-glucoside) is the major and most caharacteristic flavonoid of Filipendula ulmaria flowers and is not found in the leaves and stems. This paper describes a quantification of ... [more ▼]

Spiraeoside (quercetin-4'-glucoside) is the major and most caharacteristic flavonoid of Filipendula ulmaria flowers and is not found in the leaves and stems. This paper describes a quantification of spiraeoside by HPTLC densitometry after derivatization with diphenylboric acid -2-aminoester. The fluorescence of this flavonoid was measured at 330 nm. the spiraeoside content in batches of Filipendula ulmaria flowers ranged from 3 to 4.3 %. It is of note that these results are similar to those published elsewhere after HPLC analysis of other batches. [less ▲]

Alkaloids of Strychnos Usambarensis Stem Bark

in Planta Medica (1991), 57(5), 501

Matadine, a cytotoxic alkaloid from Strychnos gossweileri

in Phytochemistry (1991), 30(5), 1697-1700

Matadine, a new alkaloid, has been isolated from the root bark of Strychnos gossweileri. Elucidation of its structure is mainly based on 1H and 1D NMR studies. Its cytotoxic activity has been tested on ... [more ▼]

Matadine, a new alkaloid, has been isolated from the root bark of Strychnos gossweileri. Elucidation of its structure is mainly based on 1H and 1D NMR studies. Its cytotoxic activity has been tested on cancer cells and normal cells. Matadine is an anhydronium base as serpentine, that exerts also a selective inhibiting activity on B16 melanoma cells while it is less toxic in human 2002 non-cancer cells. This selective activity might be well due , as it seems to be the case for serpentine and alstonine, to a higher affinity of matadine for destabilized single-stranded DNA as mainly present in cancer cells. [less ▲]

Les Territoires Méconnus de la Pharmacognosie

Article for general public (1991)

Revision of the Structure of Strychnochromine

in Tetrahedron Letters (1991), 32(34), 4295-4298

The structure of strychnochromine, an unusual alkaloid isolated from a new batch of the root bark of Strychnos gossweileri has been revised . To confirm the indoline structure ( proposed in 1988) and to ... [more ▼]

The structure of strychnochromine, an unusual alkaloid isolated from a new batch of the root bark of Strychnos gossweileri has been revised . To confirm the indoline structure ( proposed in 1988) and to establish the stereochemistry of the compound extensive 2D-NMR experiments were carried out. The COSY experiments mostly confirmed the previous observations but the COLOC maps did not fit the first proposed structure!The problem was solved by single-crystal X-ray analysis of the p-bromobenzoyl ester of strychnochromine. The new structure, with its tetrahydroquinoline ring, is very unusual in Strychnos species but the formation of quinolines from indole alkaloids has already mentioned in some other plants ( Cinchona, Alstonia, Hunteria..). [less ▲]

From ethnobotanical uses of Strychnos henningsii to antiinflammatories, analgesics and antispasmodics

in Journal of Ethnopharmacology (1991), 34

Strychnos henningsii GILG is used in African traditional medicine for the treatment of various ailments, including rheumatism, gastrointestinal complaints, malaria and snake bites. Different preliminary ... [more ▼]

Strychnos henningsii GILG is used in African traditional medicine for the treatment of various ailments, including rheumatism, gastrointestinal complaints, malaria and snake bites. Different preliminary pharmacological experiments are described. The results show that some of the reported folk medicinal applications of S. henningsii can be at least partially explained by the presence of retuline-like alkaloids, whose use could lead to new antinociceptive (antiinflammatory and analgesic) and antispasmodic drugs. [less ▲]

Effects of Strychnopentamine on Cells Cultured in Vitro

in Chemico-Biological Interactions (1991), 80(2), 203-16

This paper describes the powerful cytotoxic action exerted by strychnopentamine (SP), a dimeric indole alkaloid extracted from Strychnos usambarensis Gilg, on B16 melanoma cells and on non-cancer human ... [more ▼]

This paper describes the powerful cytotoxic action exerted by strychnopentamine (SP), a dimeric indole alkaloid extracted from Strychnos usambarensis Gilg, on B16 melanoma cells and on non-cancer human fibroblasts cultured in vitro. SP strongly inhibits cell proliferation and induces cell death at a relatively low concentration (less than 1 microgram/ml) after 72 h of treatment in the two lines. Incorporation of [3H]thymidine and [3H]leucine by B16 cells significantly decreases after only 1 h of treatment at 0.5 microgram/ml. SP induces the formation of dense lamellar bodies and vacuolization in the cytoplasm, intense blebbing at the cell surface and various cytological alterations leading to cell death. [less ▲]