References of "Albert, Adelin"
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See detailThe multivariate coefficient of variation for comparing serum protein electrophoresis techniques in External Quality Assessment schemes
Zhang, Lixin ULg; Albarède, Stéphanie; Dumont, Gilles et al

in Accreditation and Quality Assurance (2010)

External Quality Assessment (EQA) schemes are national or transnational programmes designed to control the analytical performance of clinical laboratories and to maintain inter-laboratory variability ... [more ▼]

External Quality Assessment (EQA) schemes are national or transnational programmes designed to control the analytical performance of clinical laboratories and to maintain inter-laboratory variability within acceptable limits. In such EQA programmes, participants are usually grouped by the type of assay technique/equipment they use. The coefficient of variation (CV) is a simple tool for comparing the inter-laboratory reproducibility of such techniques: the lower the CV, the better the analytical performance. Serum protein electrophoresis, a laboratory test profile consisting of five fractions (albumin, α1, α2, β and γ globulins) summing up to 100% of total proteins, can also be assayed in different ways depending on the media or the analytical principle. We propose a multivariate coefficient of variation for comparing the performance of electrophoretic techniques in EQA, thus extending the univariate CV concept. First, the compositional nature of electrophoretic data requires a one-to-one transformation from the 5-dimensional to the 4-dimensional space. Next, robust estimations of the mean and the covariance matrix are needed to avoid the effect of outliers. The new approach is illustrated on electrophoretic datasets from the French and Belgian national EQA programmes. [less ▲]

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See detailPenetration of enrofloxacin into the nasal secretions and relationship between nasal secretions and plasma enrofloxacin concentrations after intramuscular administration in healthy pigs
Bimazubute, M.; Cambier, Carole ULg; Baert, K. et al

in Journal of Veterinary Pharmacology & Therapeutics (2010), 33(2), 183-188

The pharmacokinetic behaviour of enrofloxacin (ENRO) in plasma and nasal secretions of healthy pigs was investigated, after a single-dose intramuscular administration of 2.5 mg/kg body weight of the drug ... [more ▼]

The pharmacokinetic behaviour of enrofloxacin (ENRO) in plasma and nasal secretions of healthy pigs was investigated, after a single-dose intramuscular administration of 2.5 mg/kg body weight of the drug. Blood samples and nasal secretions were collected at predetermined times after drug administration. Concentrations of ENRO and its active metabolite ciprofloxacin (CIPRO) were determined in plasma and nasal secretions by high-performance liquid chromatography (HPLC). CIPRO was not detected probably because we investigated young weaned pigs. The data collected in 12 pigs for ENRO were subjected to noncompartmental analysis. In plasma, the maximum concentration of drug (C-max), the time at which this maximum concentration of drug (T-max) was reached, the elimination half-life (t(beta)(1/2)) and the area under the concentration vs. time curve (AUC) were, respectively, 694.7 ng/mL, 1.0 h, 9.3 h and 8903.2 ng h/mL. In nasal secretions, Cmax, Tmax, t(beta)(1/2) and AUC were, respectively, 871.4 ng/mL, 2.0 h, 12.5 h and 11 198.5 ng.h/mL. In a second experiment conducted in 10 piglets, the relationship between concentrations of ENRO measured in the plasma and the nasal secretions has been determined following single-dose intramuscular administration of 2.5, 10 or 20 mg/kg body weight of the drug. It has been demonstrated that, among several variables, i.e., (1) the dose administered, (2) the time between intramuscular injection and blood sampling, (3) the age, (4) the sex, (5) the animal body weight and (6) the plasma concentration of the drug, only the latter influenced significantly the ENRO concentration in nasal secretions. Practically, using a generalized linear mixed model, ENRO concentrations in the nasal secretions (mu g/mL) can be predicted taking into account the ENRO concentrations in plasma (mu g/mL), according to the following equation: ENROnasal secretion 1.94 ENROplasma - 0.24. [less ▲]

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See detailRevisiting the multivariate coefficient of variation for comparing electrophoretic techniques in External Quality Assessment (EQA) schemes
Zhang, Lixin ULg; Albert, Adelin ULg

Conference (2009, October 15)

External Quality Assessment (EQA) schemes are national programmes designed to control the analytical performance of clinical laboratories and to maintain inter-laboratory variability within acceptable ... [more ▼]

External Quality Assessment (EQA) schemes are national programmes designed to control the analytical performance of clinical laboratories and to maintain inter-laboratory variability within acceptable limits. In this context, the type of assay technique or equipment used by the participants is an important factor to take into account. The coefficient of variation (CV) is widely used in EQA to compare the reproducibility of techniques and equipments: the lower the CV, the better the analytical performance. The recent introduction of protein electrophoresis, a laboratory test profile consisting of five fractions (albumin, α1, α2, β and γ globulins) summing up to 100%, in the EQA programme (Zhang et. al. 2008) prompted us to revisit an old problem (Reyment 1960), namely the multivariate extension of the univariate CV concept, and to suggest some amendments to existing solutions. Further, the application of the multivariate CV to techniques and methods used in the assay of protein electrophoresis raises concomitant statistical problems. First, the compositional nature of electrophoretic data requires a log-ratio one-to-one transformation from the simplex to the real space (Aitchison 1982). Next, robust estimations of the mean and the covariance matrix (Rousseeuw and Van Driessen 1999) are needed to avoid the effect of outliers. The resulting robust multivariate CVs are illustrated on electrophoretic EQA material from the Belgian and French national EQA programmes to assess the analytical variability of the electrophoretic techniques used. [less ▲]

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See detailComparaison de 5 techniques de prération du PRP (Platelet-Rich Plasma ou plasma)
Kaux, Jean-François ULg; Le Goff, Caroline ULg; Seidel, Laurence ULg et al

in Annales de Réadaptation et de Médecine Physique (2009, October), 52(Sup. 1), 109

Introduction : Depuis une vingtaine d’années, le développement des activités sportives s’accompagne d’une incidence accrue de diverses tendinopathies, souvent rebelles aux traitements conservateurs ... [more ▼]

Introduction : Depuis une vingtaine d’années, le développement des activités sportives s’accompagne d’une incidence accrue de diverses tendinopathies, souvent rebelles aux traitements conservateurs classiques (anti-inflammatoires non stéroïdiens, orthèses, kinésithérapie, infiltrations…). De nouvelles thérapeutiques, dont l’injection de concentrés plaquettaires (plasma riche en plaquettes ou PRP), sont actuellement en cours d’évaluation clinique. Objectifs : L’injection de PRP fait actuellement l’objet de recherche comme thérapeutique des tendinopathies chroniques. L’injection intra-tendineuse nécessite idéalement un volume minimal afin de diminuer la pression lors de l’injection et minimiser les douleurs, mais il doit également présenter une concentration plaquettaire élevée ; par ailleurs, la quantité de facteurs de croissance libérés pourrait être liée au système de préparation. Méthodes : Après avoir prélevés divers échantillons de sang veineux chez 5 patients, nous avons comparé 5 techniques de préparation du PRP : celle du Service d’Hématologie Biologique du CHU de Liège, le PRP Kit de Curasan®, les techniques Plateltex®, GPS®II et RegenLab®. Résultats : Les différentes techniques permettent d’obtenir des concentrations plaquettaires plus importantes que dans le sang avec des volumes variables (de 0,3 mL à 6 mL) et un nombre de globules rouges et globules blancs limité (sauf pour GPS® II). Le nombre de plaquettes/µL apparaît plus élevé avec la technique Plateltex® et obtient le plus petit volume à injecter. Les autres techniques permettent également d’obtenir de petits volumes sauf avec le GPS®II. Le nombre de plaquettes collectées dans le PRP apparaît donc plus élevé avec cette technique mais avec une concentration faible. Discussion – Conclusion : La technique décrite par Plateltex® permet de recueillir le PRP le plus concentré dans le volume le plus faible. [less ▲]

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See detailAgreement between an isolated rater and a group of raters
Vanbelle, Sophie ULg; Albert, Adelin ULg

in Statistica Neerlandica (2009), 63(1), 82-100

The agreement between two raters judging items on a categorical scale is traditionally assessed by Cohen’s kappa coefficient. We introduce a new coefficient for quantifying the degree of agreement between ... [more ▼]

The agreement between two raters judging items on a categorical scale is traditionally assessed by Cohen’s kappa coefficient. We introduce a new coefficient for quantifying the degree of agreement between an isolated rater and a group of raters on a nominal or ordinal scale. The group of raters is regarded as a whole, a reference or gold-standard group with its own heterogeneity. The coefficient, defined on a population-based model, requires a specific definition of the concept of perfect agreement. It has the same properties as Cohen’s kappa coefficient and reduces to the latter when there is only one rater in the group. The new approach overcomes the problem of consensus within the group of raters and generalizes Schouten’s index. The method is illustrated on published syphilis data and on data collected from a study assessing the ability of medical students in diagnostic reasoning when compared with expert knowledge. [less ▲]

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See detailCurrent practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme.
Van Blerk, Marjan; Van Campenhout, Christel; Bossuyt, Xavier et al

in Clinical Chemistry & Laboratory Medicine (2009), 47(1), 102-8

BACKGROUND: This study aimed to assess the state-of-the-art of antinuclear antibody (ANA) testing as practiced in the Belgian and Luxembourg laboratories, using the results obtained in the Belgian ... [more ▼]

BACKGROUND: This study aimed to assess the state-of-the-art of antinuclear antibody (ANA) testing as practiced in the Belgian and Luxembourg laboratories, using the results obtained in the Belgian National External Quality Assessment Scheme from 2000 to 2005. METHODS: During this period, nine samples with different specificities were sent for analysis. Participants were surveyed for methodology used and were asked to report staining pattern and titer of ANAs. In 2002, an attempt was made to improve the comparability of quantitative ANA results by the provision of a commercial reference material and to relate observed differences to methodology. RESULTS: With one exception, all participants employed a microscope-based indirect immunofluorescence assay with human epithelial cell line 2 cells. Most laboratories were accurate in describing the pattern. The percentage of unacceptable answers was greater for samples with borderline levels of antibody and for samples showing a cytoplasmic pattern. An improvement in the detection of anticentromere antibodies was observed. For all samples, a wide range of titers was reported. The provision of the secondary reference preparation led to improved inter-laboratory concordance. Comparison of methodology variables revealed a correlation between unstandardized titers and the power of the lamp of the microscope and the use of a dark room. CONCLUSIONS: The EQAS results presented in this work provide valuable insights into the state of the art of ANA testing as practiced in the Belgian and Luxembourg Laboratories and illustrate the important value of a national EQAS for ANA testing as a tool to improve performance and interlaboratory comparability of laboratory results. [less ▲]

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See detailAlcohol consumption and the prevalence of metabolic syndrome : a meta-analysis of observational studies
Alkerwi, A; Boutsen, M; Vaillant, M et al

Poster (2009)

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See detailEtude épidémiologique des comportements alimentaires au service de la promotion de la santé
Lair, ML; Alkerwi, A; Couffignal, S et al

Conference (2009)

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See detailAlcohol consumption and the prevalence of metabolic syndrome: a meta-analysis of observational studies.
Alkerwi, Ala'a; Boutsen, Michel; Vaillant, Michel et al

in Atherosclerosis (2009), 204(2), 624-35

BACKGROUND: In the past two decades, the metabolic syndrome has given rise to much clinical and research interest. The broad overlap of alcohol consumption with different components of metabolic syndrome ... [more ▼]

BACKGROUND: In the past two decades, the metabolic syndrome has given rise to much clinical and research interest. The broad overlap of alcohol consumption with different components of metabolic syndrome makes alcohol-metabolic syndrome relationship a controversial topic. OBJECTIVES: To support the evidence available about the relationship between alcohol consumption and metabolic syndrome as a comprehensive clinical entity, as well as to identify the gender-specific dose-response, by performing a meta-analysis based on information from published data. METHODS: Manual and computer searches in different bibliographic databases were performed to identify the relevant scientific publications, on the relation between alcohol consumption and metabolic syndrome. Alcohol intake was converted into a same unit (g/day) and then categorized using standard classification in order to provide relevant comparisons. Fixed and random effects models were used to aggregate individual odds ratios and to derive pooled estimates and 95% confidence intervals. RESULTS: Fourteen relevant publications were identified on the relation between alcohol consumption and the prevalence of metabolic syndrome. 7 studies were included in the meta-analysis. The results showed that alcohol consumption of less than 40 g/day in men and 20 g/day in women significantly reduced the prevalence of metabolic syndrome. CONCLUSION: "Responsible alcohol intake" appears to be associated with a reduced prevalence of metabolic syndrome. Favorable metabolic effect seemed to be restricted to alcohol consumption of less than 20 g/day among women, and of less than 40 g/day among men. These findings support the actual recommendations regarding alcohol consumption among apparently healthy people. [less ▲]

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See detailAgreement between two independent groups of raters
Vanbelle, Sophie ULg; Albert, Adelin ULg

in Psychometrika (2009), 74

We propose a coefficient of agreement to assess the degree of concordance between two independent groups of raters classifying items on a nominal scale. This coefficient, defined on a population-based ... [more ▼]

We propose a coefficient of agreement to assess the degree of concordance between two independent groups of raters classifying items on a nominal scale. This coefficient, defined on a population-based model, extends the classical Cohen's kappa coefficient for quantifying agreement between two raters. Weighted and intraclass versions of the coefficient are also given and their sampling variance is determined by the Jackknife method. The method is illustrated on medical education data which motivated the research. [less ▲]

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See detailEarly termination of ISRCTN45828668, a phase 1/2 prospective, randomized study of sulfasalazine for the treatment of progressing malignant gliomas in adults.
Robe, Pierre ULg; Martin, Didier ULg; Nguyen-Khac, Minh-Tuan ULg et al

in BMC Cancer (2009), 9

BACKGROUND: Sulfasalazine, a NF-kappaB and x(c)-cystine/glutamate antiport inhibitor, has demonstrated a strong antitumoral potential in preclinical models of malignant gliomas. As it presents an ... [more ▼]

BACKGROUND: Sulfasalazine, a NF-kappaB and x(c)-cystine/glutamate antiport inhibitor, has demonstrated a strong antitumoral potential in preclinical models of malignant gliomas. As it presents an excellent safety profile, we initiated a phase 1/2 clinical study of this anti-inflammatory drug for the treatment of recurrent WHO grade 3 and 4 astrocytic gliomas in adults. METHODS: 10 patients with advanced recurrent anaplastic astrocytoma (n = 2) or glioblastoma (n = 8) aged 32-62 years were recruited prior to the planned interim analysis of the study. Subjects were randomly assigned to daily doses of 1.5, 3, 4.5, or 6 grams of oral sulfasalazine, and treated until clinical or radiological evidence of disease progression or the development of serious or unbearable side effects. Primary endpoints were the evaluation of toxicities according to the CTCAE v.3.0, and the observation of radiological tumor responses based on MacDonald criteria. RESULTS: No clinical response was observed. One tumor remained stable for 2 months with sulfasalazine treatment, at the lowest daily dose of the drug. The median progression-free survival was 32 days. Side effects were common, as all patients developed grade 1-3 adverse events (mean: 7.2/patient), four patients developed grade 4 toxicity. Two patients died while on treatment or shortly after its discontinuation. CONCLUSION: Although the proper influence of sulfasalazine treatment on patient outcome was difficult to ascertain in these debilitated patients with a large tumor burden (median KPS = 50), ISRCTN45828668 was terminated after its interim analysis. This study urges to exert cautiousness in future trials of Sulfasalazine for the treatment of malignant gliomas. TRIAL REGISTRATION: Current Controlled Trials ISRCTN45828668. [less ▲]

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See detailA note on the linearly weighted kappa coefficient for ordinal scales
Vanbelle, Sophie ULg; Albert, Adelin ULg

in statistical methodology (2009), 6

A frequent criticism formulated against the use of weighted kappa coeffcients is that the weights are arbitrarily defined. We show that using linear weights for a K-ordinal scale is equivalent to deriving ... [more ▼]

A frequent criticism formulated against the use of weighted kappa coeffcients is that the weights are arbitrarily defined. We show that using linear weights for a K-ordinal scale is equivalent to deriving a kappa coeffcient from K-1 embedded 2 x 2 tables. [less ▲]

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See detailImpact of bone marker feedback on adherence to once monthly ibandronate for osteoporosis among Asian postmenopausal women
Kung, Annie Wai-Chee; Rachman, A Ichramsjah; Adam, MF John et al

in International Journal of Rheumatic Diseases (2009), 12

Aim: This study assesses the impact of serum carboxy-terminal collagen crosslinks (CTX) bone marker feedback (BMF) on adherence to ibandronate treatment in Asian postmenopausal women with osteoporosis ... [more ▼]

Aim: This study assesses the impact of serum carboxy-terminal collagen crosslinks (CTX) bone marker feedback (BMF) on adherence to ibandronate treatment in Asian postmenopausal women with osteoporosis. Methods: This was a 12-month (6-monthly phased), randomized, prospective, open-label, multi-center study conducted in 596 (of 628 enrolled) postmenopausal women with osteoporosis (£ 85 years old) who were naı¨ve, lapsed, or current bisphosphonate users. Patients were randomized into two arms: serum CTX BMF at 3 months versus no-BMF. Once-monthly 150 mg ibandronate tablet was administered for 12 months and adherence to therapy was assessed at 6 and 12 months. In addition, patient satisfaction and safety of ibandronate treatment were also assessed. Results: Serum CTX BMF at 3 months showed no impact on adherence. The proportions of adherent patients were comparable in the BMF versus no-BMF arms (92.6% vs. 96.0%, P = 0.16); overall, serum CTX levels were similar for adherent and non-adherent patients. However, BMF patients felt more informed about their osteoporosis (P < 0.001) and more satisfied (P < 0.01) than no-BMF patients. Conclusions: The Asian postmenopausal osteoporosis patients in this study had a high adherence rate to once-monthly ibandronate therapy. Use of serum CTX BMF had no further impact on increasing adherence, but increased treatment satisfaction. [less ▲]

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See detailA bootstrap method for comparing correlated kappa coefficients
Vanbelle, Sophie ULg; Albert, Adelin ULg

in Journal of Statistical Computation & Simulation (2008), 78(11), 1009-1015

Cohen’s kappa coefficient is traditionally used to quantify the degree of agreement between two raters on a nominal scale. Correlated kappas occur in many settings (e.g., repeated agreement by raters on ... [more ▼]

Cohen’s kappa coefficient is traditionally used to quantify the degree of agreement between two raters on a nominal scale. Correlated kappas occur in many settings (e.g., repeated agreement by raters on the same individuals, concordance between diagnostic tests and a gold standard) and often need to be compared. While different techniques are now available to model correlated κ coefficients, they are generally not easy to implement in practice. The present paper describes a simple alternative method based on the bootstrap for comparing correlated kappa coefficients. The method is illustrated by examples and its type I error studied using simulations. The method is also compared with the generalized estimating equations of the second order and the weighted least-squares methods. [less ▲]

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See detailStatistical analysis of serum protein electrophoresis results in External Quality Assessment schemes
Zhang, Lixin ULg; Van Campenhout, Christel; Devleeschouwer, Nicole et al

in Accreditation and Quality Assurance (2008), 13(3), 149-155

The goal of External Quality Assessment (EQA) schemes is to ensure that results obtained on a particular specimen in a given clinical laboratory are compatible with those obtained by other laboratories on ... [more ▼]

The goal of External Quality Assessment (EQA) schemes is to ensure that results obtained on a particular specimen in a given clinical laboratory are compatible with those obtained by other laboratories on the same specimen. Serum protein electrophoresis is a laboratory test consisting of five fractions (albumin, a1, a2, b and c globulins), which sum up to 100% of total proteins. So far, in EQA schemes the five fractions have been analyzed separately as for ordinary tests like glucose or cholesterol. This approach does not consider the fractions as a whole and the linear relationship between them. A statistical approach has been developed to analyze EQA electrophoresis results from a global standpoint by using robust multivariate method to eliminate the effect of outlying profiles. As illustrated on electrophoretic data from the Belgian EQA scheme, the novel approach improves the detection of poor performing laboratories. The method will be implemented in the Belgian EQA scheme on a routine basis. [less ▲]

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See detailBrief introduction to the use of the STATISTICA software
Albert, Adelin ULg; Donneau, Anne-Françoise ULg; Zhang, Lixin

Learning material (2008)

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See detailDétermination d'un index prédictif de la preeclampsie en préconceptionnel et propositions thérapeutiques de prévention primaire
Emonts, Patrick ULg; Seaksan, Sontera; Seidel, Laurence ULg et al

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2008), 37(5), 469-476

Objective To derive a prediction index based on the most salient history, laboratory and clinical parameters for identifying women at high risk of developing preeclampsia (PE) and to suggest a primary ... [more ▼]

Objective To derive a prediction index based on the most salient history, laboratory and clinical parameters for identifying women at high risk of developing preeclampsia (PE) and to suggest a primary prevention. Material and method Non-pregnant women with a history of PE (n =101) were compared to non-pregnant parous women with a history of one or more successful normotensive pregnancies (n =50) but with comparable age, gestation and parity profiles. The parameters included history and clinical examination; laboratory studies (hemostasis, coagulation, vitamins); and morphological and functional tests (cardiovascular and renal functions). Stepwise logistic regression analysis was applied to develop a three step PE prediction index based on the most discriminant parameters. Strategies to prevent PE in the high-risk group are described. Results Identification of women at high risk of PE can be done efficiently (88% sensitivity and specificity) using a predictive index based on a simple history, laboratory, clinical and functional information. Stategies to prevent PE in our high-risk group have given encouraging results during next pregnancy. Conclusion Our study gives a predictive index of PE outside of pregnancy and possibilities to do a primary prevention. [less ▲]

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See detailComparison between player specialization, anthropometric characteristics and jumping ability in top-level volleybal players
Ciccarone, Guido; Croisier, Jean-Louis ULg; Fontani, G. et al

in Medicina dello Sport : Rivista di Fisiopatologia dello Sport (2008), 61

Aim. Volleyball is an open skill sport with predominant anaerobic alactic acid power. At higher skill levels, performance characteristics are mainly determined by speed and vertical jumping ability. The ... [more ▼]

Aim. Volleyball is an open skill sport with predominant anaerobic alactic acid power. At higher skill levels, performance characteristics are mainly determined by speed and vertical jumping ability. The aim of this study was to compare anthropometric parameters and jumping ability in a group of elite male volleyball players in relation to technical skills required by player position. Methods. The study population included 36 elite male volleyball players (coming from the Italian First and Second Division Championships). Subjects were grouped by playing position on the court: setters (7); blockers (10); hitters (16); liberos (3). Assessment included anthropometric parameters such as height, weight, body mass index (weight in kg/height in m2), indirect calculation of body fat mass by means of the Katch method (%BFM), measurement of reach with one hand (R1) and two hands (R2); and indirect measurement of explosive strength by means of the Bosco method: squat jump, countermovement jump (CMJ), CMJ with arm swing (CMJas), and repeated jumps for 15 s (R15”); measurement of motor coordination and jumping ability by means of the Vertec test combined with specific offense (Vertec attack [VA]) and defense (Vertec block [VB]) skills. Results. Significant differences were observed between player specialization and anthropometric parameters (height, weight, BMI, R1 and R2). Results of the Bosco (SJ, CMJ, CMJas) and Vertec tests (VA and VB) also differed significantly among the player groups. Conclusion. Functional assessment protocols that evaluate anthropometric characteristics and jumping ability by means of two different unrelated methods, confirming that the two measure different aspects of the same performance, provide more complete indications for selecting players, workload planning and monitoring of player development during training. [less ▲]

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