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See detailCurcumin inhibits pro-inflammatory mediators and metalloproteinase-3 production by chondrocytes
Mathy, Marianne ULg; Jacquemond-Collet, Ingrid; Priem, Fabian et al

in Inflammation research (2009), 58

Objective and Design. This study aims to investigate the effects of curcumin (Cur) on the production of inflammatory mediators and on the extracellular matrix proteins metabolism by articular chondrocytes ... [more ▼]

Objective and Design. This study aims to investigate the effects of curcumin (Cur) on the production of inflammatory mediators and on the extracellular matrix proteins metabolism by articular chondrocytes. Methods. Human chondrocytes in alginate beads and human cartilage explants were cultured in the absence or in the presence of interleukin (IL)-1beta(10-11 M) and with or without Cur (5 to 20 µM). Nitric oxide (NO) synthesis was measured by Griess spectrophotometric method, prostaglandin (PG) E2 by a specific radioimmunoassay and IL-6, IL-8, aggrecan (Agg), matrix metalloproteinase (MMP)-3 and tissue inhibitor of metalloproteinase (TIMP)-1 by specific enzyme-amplified immunoassays. Proteoglycans degradation was evaluated by the release of 35S-glycosaminoglycans (GAG) from human cartilage explants. Results. In alginate beads and cartilage explants models, Cur inhibited in a concentration dependent manner the basal and the IL-1beta stimulated NO, PGE2, IL-6, IL-8 and MMP-3 production by human chondrocytes. The TIMP-1 and the Agg productions were not modified. In basal condition, 35S-GAG release from cartilage explants was decreased by Cur. Conclusions. Cur was a potent inhibitor of the production of inflammatory and catabolic mediators by chondrocytes, suggesting that this natural compound could be efficient in the treatment of osteoarthritis. [less ▲]

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See detailSet-up of Z- and E-guggulsterones purification method from guggul-gum extracts by liquid chromatography and study of their activities on human preadipocytes differentiation
Hanon, Emilien ULg; Keophiphath, Mayoura; Jacquemond-Collet, Ingrid et al

Poster (2009)

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and ... [more ▼]

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and cardiovascular diseases which nowadays rank among the highest causes of premature death. Therefore, modulating fat mass expansion represents a worldwide challenge. In this context, adipocyte differentiation, which corresponds to the cellular transition of a fibroblastic cell (the preadipocyte) to a highly specialized cell accumulating triglycerides (the adipocyte), is a decisive process in the expansion of adipose tissue during life span and consequently, in the development of obesity. Adipogenesis markers such as PPARg2, C/EBPa, lipoprotein lipase (LPL), perilipin (PLIN) and the adipokines (leptin and adinopectin) are known to be expressed throughout the different stages of adipocyte differentiation. Guggulsterones are the principal bioactive steroidal components found in the oleoresin (guggul gum) collected from the indian guggul tree, Commiphora mukul (Hook, ex Stocks) Engl. The objective of this study was to investigate the effects of the two purified diastereoisomeric forms of guggulsterones (Z- and E-) on the human preadipocytes proliferation and differentiation. [less ▲]

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See detailThe effects of purified Z- and E-guggulsterones from guggul-gum extract on human preadipocytes proliferation and differentiation through the study of gene expression of adipose markers.
Hanon, Emilien ULg; Keophiphath, Mayoura; Jacquemond-Collet, Ingrid et al

Poster (2009)

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and ... [more ▼]

The rising prevalence of obesity within both industrialized and emerging societies is a major public health problem. Indeed obesity is a usual risk factor in the development of metabolic and cardiovascular diseases which nowadays rank among the highest causes of premature death. Therefore, modulating fat mass expansion represents a worldwide challenge. In this context, adipocyte differentiation, which corresponds to the cellular transition of a fibroblastic cell (the preadipocyte) to a highly specialized cell accumulating triglycerides (the adipocyte), is a decisive process in the expansion of adipose tissue during life span and consequently, in the development of obesity. Adipogenesis markers such as PPARg2, C/EBPa, lipoprotein lipase (LPL), perilipin (PLIN) and the adipokines (leptin and adinopectin) are known to be expressed throughout the different stages of adipocyte differentiation. Guggulsterones are the principal bioactive steroidal components found in the oleoresin (guggul gum) collected from the indian guggul tree, Commiphora mukul (Hook, ex Stocks) Engl. The objective of this study was to investigate the effects of the two purified diastereoisomeric forms of guggulsterones (Z- and E-) on the human preadipocytes proliferation and differentiation. [less ▲]

Detailed reference viewed: 63 (7 ULg)