Use of an Automatic DNA Sequencer for S1 Mapping: Transcriptional Analysis of the Streptomyces Coelicolor A3(2) Dnak OperonBrans, Alain ; ; Thamm, Iris et alin FEMS Microbiology Letters (1997), 149(2), 189-94 The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond ... [more ▼] The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters. [less ▲] Detailed reference viewed: 32 (8 ULg) Cloning and Sequencing of the Dnak Locus in Streptomyces Coelicolor A3(2)Brans, Alain ; ; Joris, Bernard et alin DNA Sequence : The Journal of DNA Sequencing & Mapping (1996), 6(3), 179-84 The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK ... [more ▼] The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK proteins (HSP70). The isolated insert-a BamHI 5.6-kb fragment-was sequenced and shown to contain three open-reading frames organized in an operon and coding for proteins analogous to DnaK, GrpE and DnaJ, successively. [less ▲] Detailed reference viewed: 18 (7 ULg) |
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