References of "Loret, Suzanne"
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See detailAlkaline hydrolysis for animal carcass disposal: a regulatory challenge
Van der Meulen, Karen; Custer, René; Houthoofd, Koen et al

Conference (2014, May)

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See detailPreliminary characterization of jejunocyte and colonocyte cell lines isolated by enzymatic digestion from adult and young cattle.
Loret, Suzanne; Rusu, Dorina; Moualij, Benaissa El et al

in Research in Veterinary Science (2009), 87(1), 123-32

In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be ... [more ▼]

In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be distinguished from simultaneously isolated colonocytes, as the jejunocytes specifically exhibited the small intestinal peptidase gene transcript, as well as an active alkaline phosphatase. The transformation of both types of cell suspension was performed by retroviral infection, using reproduction-defective viruses bearing the gene coding for the large T antigen of the leukaemia simian virus (SV40). The success of the transfection was demonstrated by (1) a significant increase in cell passage numbers (52-53 vs. 7 passages for non-transfected cells), (2) the detection of both the large T transcript and the large T antigen in transformed cells. Possible contamination and progressive substitution of bovine primocultures by non-bovine lineages available in the laboratory was excluded, as the transformed cells presented a bovine typical karyotype. Most transfected cells kept an epithelial morphology after transformation. They also maintained the expression of FABP and enterocyte specific enzymes (brush-border associated maltase and IAP). However, levels of specific activity of these enzymes were low, suggesting that cell differentiation is not completely achieved under the applied culture conditions. [less ▲]

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See detailPolyamines as essential substances in metabolism
Dandrifosse, Anne-catherine; Deloyer, Patricia; Loret, Suzanne et al

in Trends in Comparative Biochemistry and Physiology (2002), 9

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See detailPolycations : relationship between chemical structure and biological behavior
Grandfils, Christian ULg; Peulen, Olivier ULg; Deloyer, Patricia et al

in Morgan, D.; White, A.; Sanchez-Jimenez, F. (Eds.) et al COST 917 Biologically active amines in food. Volume IV (2000)

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See detailAPH-1, a POU homeobox gene expressed in the salt gland of the crustacean Artemia franciscana
Chavez, Marcela; Landry, Claire; Loret, Suzanne et al

in Mechanisms of Development (1999), 87(1-2), 207-12

We characterized the first POU-homeoprotein in a crustacean (designated APH-1 for Artemia POU-Homeoprotein, EMBL Y15070). The amino acid sequence of the APH-1 POU-domain is identical, except for two ... [more ▼]

We characterized the first POU-homeoprotein in a crustacean (designated APH-1 for Artemia POU-Homeoprotein, EMBL Y15070). The amino acid sequence of the APH-1 POU-domain is identical, except for two residues, to that of the two class III POU proteins Cf1-a (Drosophila) and POU-M1 (Bombyx mori). Southern blot analysis suggests that crustaceans have only one class III POU gene. RT-PCR and whole-mount in situ hybridization show that APH-1 mRNA is present in larvae specifically in the salt gland, an organ which is involved in osmoregulation, and disappears in the adult. [less ▲]

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