References of "Loret, S"
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See detailImmunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures
Rusu, D.; Loret, S.; Peulen, Olivier ULg et al

in BMC Cell Biology (2005), 6

Background: Cultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs ... [more ▼]

Background: Cultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs, interactions of enteropathogenes bacteria strains with intestinal epithelium and other physiologic or pathologic phenomenon involving the digestive tract. Results: Cultures of bovine colonocytes and jejunocytes were obtained from organoid-enriched preparations, using a combination of enzymatic and mechanical disruption of the intestine epithelium, followed by an isopicnic centrifugation discarding most single cells. Confluent cell monolayers arising from plated organoids exhibited epithelium typical features, such as the pavement-like structure, the presence of apical microvilli and tight junctions. Accordingly, cells expressed several markers of enterocyte brush border (i.e. maltase, alkaline phosphatase and fatty acid binding protein) as well as an epithelial cytoskeleton component (cytokeratin 18). However, enterocyte primocultures were also positive for the vimentin immunostaining (mesenchyme marker). Vimentin expression studies showed that this gene is constitutively expressed in bovine enterocytes. Comparison of the vimentin expression profile with the pattern of brush border enzymes activities, suggested that the decrease of cell differentiation level observed during the enterocyte isolation procedure and early passages of the primoculture could result from a post-transcriptional de-repression of vimentin synthesis. The low differentiation level of bovine enterocytes in vitro could partly be counteracted adding butyrate (1-2 mM) or using a glucose-deprived culture medium. Conclusion: The present study describes several complementary approaches to characterize bovine primary cultures of intestinal cells. Cultured cells kept their morphologic and functional characteristics during several generations. [less ▲]

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See detailAnalysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection
Loret, S.; Nollevaux, G.; Remacle, R. et al

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2004), 805(2), 195-202

We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly ... [more ▼]

We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C 18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20 +/- 9.30% and 0.12 +/- 0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52 +/- 0.35 expressing a low diffusion of this poorly permeability marker. (C) 2004 Published by Elsevier B.V. [less ▲]

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See detailUse of fluorescent polyethylene glycols (PEGs) to assess intestinal permeability in vitro
Loret, S; Pirson, L; Nollevaux, G et al

in Pflügers Archiv : European Journal of Physiology (2001, September)

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See detailUse of fluorescent polyethylene glycols (PEGs) to assess intestinal permeability in vitro
Loret, S; Pirson, L; Nollevaux, G et al

in Pflügers Archiv : European Journal of Physiology (2001), 442(5), 187

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See detailUrinary excretion of high Mr PEG (4000 Da) in rats
Nollevaux, G; Loret, S; Barakat, I et al

Conference (2000, November 18)

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See detailUrinary excretion of high Mr PEG (>4000 Da) in rats
Nollevaux, G; Loret, S; Barakat, I et al

Poster (2000, November 18)

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See detailBiocompatibility of circulating polyethylene glycols (PEG): an approach using bovine serum albumin (BSA) as a protein model
Loret, S; Gilson, Ph; Barakat, I et al

Poster (2000, September 19)

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See detailBiocompatibility of polyethylene glycols (PEG): An approach using bovine serum albumin (BSA) as a protein model.
Loret, S; Gilson, P; Barakat, I et al

in Pflügers Archiv : European Journal of Physiology (2000), 440

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See detailPolycations: relationship between chemical structure and biological behaviour
Grandfils, Christian ULg; Peulen, Olivier ULg; Deloyer, P et al

in Biologically active amines in food (2000)

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See detailIntestinal maturation induced by spermine in young animals
Peulen, Olivier ULg; Deloyer, P; Grandfils, Christian ULg et al

in Livestock Production Science (2000), 66

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See detailLe point sur les polyamines
Dandrifosse, Guy ULg; Deloyer, P.; Grandfils, Christian ULg et al

in Revue Médicale de Liège (1999), 54(3), 175-83

Polyamines are ubiquitous substances. Their intracellular concentration is controlled quickly and rigorously by extremely sophisticated systems. It depends on metabolism and cellular permeability ... [more ▼]

Polyamines are ubiquitous substances. Their intracellular concentration is controlled quickly and rigorously by extremely sophisticated systems. It depends on metabolism and cellular permeability. Polyamines act as structural and functional elements in the cell (nucleic acid conformation, cytoskeleton, radioprotection, apoptosis, proliferation and differentiation of cells...). They also play a role in various diseases (origin of food allergy, cancers...). They present a great therapeutic interest (oncology, molecular transfer to cell nucleus, transfer across the blood-brain barrier, parasitosis, effects on NMDA and GABA receptors in the central nervous system...). [less ▲]

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See detailBiogenic amines in different types of Belgian beers: a first survey
Loret, S.; Bianchi, D.; Peulen, Olivier ULg et al

in Bardocz, S.; Koninckx, J.; Grillo, M. (Eds.) et al COST 917 Biogenically active amines in food. Volume III : Biologically active amines in food processing and amines produced by bacteria, and polyamines and tumour growth (1999)

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