PMID- 3548700
OWN - NLM
STAT- MEDLINE
DA  - 19870401
DCOM- 19870401
LR  - 20091118
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 239
IP  - 3
DP  - 1986 Nov 1
TI  - Properties of a class C beta-lactamase from Serratia marcescens.
PG  - 581-6
AB  - A beta-lactamase produced by a penicillin-resistant strain of Serratia marcescens
      was isolated and purified. The kcat. value for benzylpenicillin was about 5% of
      that observed for the best cephalosporin substrates. However, the low Km of the
      penam resulted in a high catalytic efficiency (kcat./Km) and the classification
      of the enzyme as a cephalosporinase might not be completely justified. It also
      exhibited a low but measurable activity against cefotaxime, cefuroxime, cefoxitin
      and moxalactam. Substrate-induced inactivation was observed both with a very good
      (cephalothin) or a very bad (moxalactam) substrate. The active site was labelled 
      by beta-iodopenicillanate. Trypsin digestion produced a 19-residue active-site
      peptide whose sequence clearly allowed the classification of the enzyme as a
      class C beta-lactamase.
FAU - Joris, B
AU  - Joris B
FAU - De Meester, F
AU  - De Meester F
FAU - Galleni, M
AU  - Galleni M
FAU - Masson, S
AU  - Masson S
FAU - Dusart, J
AU  - Dusart J
FAU - Frere, J M
AU  - Frere JM
FAU - Van Beeumen, J
AU  - Van Beeumen J
FAU - Bush, K
AU  - Bush K
FAU - Sykes, R
AU  - Sykes R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - ENGLAND
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Amino Acids)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Peptide Fragments)
RN  - EC 3.5.2.6 (AmpC beta-lactamases)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Amino Acids/analysis
MH  - *Bacterial Proteins
MH  - Binding Sites
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Osmolar Concentration
MH  - Peptide Fragments/analysis
MH  - Serratia marcescens/*enzymology
MH  - beta-Lactamases/*isolation & purification/metabolism
PMC - PMC1147326
OID - NLM: PMC1147326
EDAT- 1986/11/01
MHDA- 1986/11/01 00:01
CRDT- 1986/11/01 00:00
PST - ppublish
SO  - Biochem J. 1986 Nov 1;239(3):581-6.